KR960702865A - PUTRESCINE N-METHYLTRANSFERA-SE, RECOMBINANT DNA MOLECULES ENCODING PUTRESCINE N-METHYLTRANFERASE, AND TRANSGENIC TOBACCO PLANTS WITH DECREASED ALKALOID CONTENT) - Google Patents

PUTRESCINE N-METHYLTRANSFERA-SE, RECOMBINANT DNA MOLECULES ENCODING PUTRESCINE N-METHYLTRANFERASE, AND TRANSGENIC TOBACCO PLANTS WITH DECREASED ALKALOID CONTENT) Download PDF

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KR960702865A
KR960702865A KR1019950705408A KR19950705408A KR960702865A KR 960702865 A KR960702865 A KR 960702865A KR 1019950705408 A KR1019950705408 A KR 1019950705408A KR 19950705408 A KR19950705408 A KR 19950705408A KR 960702865 A KR960702865 A KR 960702865A
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South Korea
Prior art keywords
putrescine
seq
recombinant dna
dna molecule
nucleotide sequence
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KR1019950705408A
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Korean (ko)
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사미르 제트. 워헵
베드팔 에스. 말리크
Original Assignee
에이. 스테판 로버츠
필립모리스 프러덕츠 인코퍼레이티드
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Publication of KR960702865A publication Critical patent/KR960702865A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

Abstract

본 발명은 고순도로 정제된 담배 퓨트레신 N-메틸트랜스퍼라제, 그의 정제방법, 및 PMT DNA 시퀀스 제조에 관한 것이다. 본 발명의 정제방법은 담배 뿌리 추출물을 음이온 교환 배지에 도포하고, 퓨트레신을 함유하는 용리 완충액으로 퓨트레신 N-메틸트랜스퍼라제를 특정하게 용리하는 것으로 이루어진다.The present invention relates to high purity purified tobacco putrescine N-methyltransferase, a purification method thereof, and preparation of PMT DNA sequence. The purification method of the present invention consists in applying a tobacco root extract to an anion exchange medium and specifically eluting putrescine N-methyltransferase with an elution buffer containing putrescine.

Description

퓨트레신 N-메틸트랜스퍼라제와, 이를 코딩하는 재조합 DNA 분자 및 알칼로이드 함량이 감소된 유전자 변이된 담배 식물(PUTRESCINE N-METHYLTRANSFERASE, RECOMBINANT DNA MOLECULES ENCODING PUTRESCINE N-METHYLTRANFERASE, AND TRANSGENIC TOBACCO PLANTS WITH DECREASED ALKALOID CONTENT)PUTRESCINE N-METHYLTRANSFERASE, RECOMBINANT DNA MOLECULES ENCODING PUTRESCINE N-METHYLTRANFERASE, AND TRANSGENIC TOBACCO PLANENT WITH DECREASED ALKALO )

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

퓨트레신의 δ-아미노기로 S-아데노실메티오닌의 메틸기를 전이하는 것을 촉매하는 작용을 특징으로 하는 담배 단백질을 코드하는 재조합 DNA 분자.A recombinant DNA molecule encoding a tobacco protein characterized by catalyzing the transfer of the methyl group of S-adenosylmethionine to the δ-amino group of putrescine. 제1항에 있어서, SEQ ID NO : 1, SEQ ID NO : 2, 또는 SEQ ID NO : 3 중 하나의 아미노산 시퀀스를 가지는 퓨트레신 N-메딜트랜스퍼라제를 코드하는 재조합 DNA 분자.The recombinant DNA molecule of claim 1, encoding putrescine N-medyltransferase having the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3. 8. 제1항에 있어서, 플라스미드 PMT14-3의 cDNA 삽입물의 뉴클레오티드 시퀀스, 또는 상기 DNA 삽입물이나 상기 뉴클레오티드 시퀀스 중 하나로 축퇴하는 뉴클레오티드 시퀀스와 혼성화하는 뉴클레오티드 시퀀스로 이루어지는 것인 재조합 DNA 분자.The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule comprises a nucleotide sequence of a cDNA insert of plasmid PMT14-3 or a nucleotide sequence that hybridizes with the DNA insert or a nucleotide sequence degenerate to one of the nucleotide sequences. 제1항에 있어서, SEQ ID NO : 17의 뉴클레오티드 시퀀스, 또는 상기 뉴클레오티드 시퀀스에 혼성화할 수 있는 DNA 시퀀스로 이루어진 재조합 DNA 분자.The recombinant DNA molecule of claim 1, wherein the recombinant DNA molecule consists of a nucleotide sequence of SEQ ID NO: 17 or a DNA sequence capable of hybridizing to the nucleotide sequence. 단리된 DNA 분자에 의해 코드되는 mRNA의 전사를 실시할 수 있는 시퀀스에 실시가능하게 결합된 제2항 내지 제4항 중 어느 하나의 항에 따른 DNA 분자를 함유하는 벡터.A vector containing a DNA molecule according to any one of claims 2 to 4 operatively linked to a sequence capable of transcription of an mRNA encoded by an isolated DNA molecule. 제5항에 있어서, 상보 안티샌스 mRNA의 전사를 실시할 수 있는 시퀀스에 결합된 상기 안티센스 mRNA를 코드하는 DNA 시퀀스를 특징으로 하는 벡터.6. The vector according to claim 5, wherein said vector encodes said antisense mRNA bound to a sequence capable of transcription of complementary antisense mRNA. 제5항 또는 제6항에 따른 벡터로 안정하게 형질전환된 배양된 유전자 변이 담배 세포.A cultured genetically modified tobacco cell stably transformed with the vector according to claim 5. 제5항 또는 제6항에 따른 벡터로 안정하게 형질전환된 유전자 변이 담배 식물.Genetically modified tobacco plant stably transformed with the vector according to claim 5. SEQ ID NO : 17에 의해 코드되는 퓨트레신 N-메틸트랜스퍼라제.Putrescine N-methyltransferase encoded by SEQ ID NO: 17. SEQ ID NO : 1, SEQ ID NO : 2 또는 SEQ ID NO : 3에 의해 코드되는 퓨트레신 N-메틸트랜스퍼라제.Putrescine N-methyltransferase encoded by SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950705408A 1993-06-01 1994-06-01 PUTRESCINE N-METHYLTRANSFERA-SE, RECOMBINANT DNA MOLECULES ENCODING PUTRESCINE N-METHYLTRANFERASE, AND TRANSGENIC TOBACCO PLANTS WITH DECREASED ALKALOID CONTENT) KR960702865A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7668193A 1993-06-01 1993-06-01
US08/076,681 1993-06-01
PCT/US1994/006106 WO1994028142A1 (en) 1993-06-01 1994-06-01 Putrescine n-methyltransferase, recombinant dna molecules encoding putrescine n-methyltransferase, and transgenic tobacco plants with decreased alkaloid content

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Publication Number Publication Date
KR960702865A true KR960702865A (en) 1996-05-23

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EP (1) EP0701614A1 (en)
JP (1) JPH09502601A (en)
KR (1) KR960702865A (en)
CN (1) CN1127530A (en)
BR (1) BR9406734A (en)
WO (1) WO1994028142A1 (en)

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US6586661B1 (en) * 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
IL159213A0 (en) * 2001-06-08 2004-06-01 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
WO2004081534A2 (en) * 2003-03-12 2004-09-23 Assay Designs, Inc. Phosphokinase assay
CA2608429A1 (en) 2005-05-11 2006-11-23 Anthony Albino Reduced risk tobacco products and methods of making same
CN1884545B (en) * 2005-06-24 2011-11-09 西南师范大学 Method for cultivating Atropa belladonna L of high tropane analog alkaloid yield using gene engineering technology and Atropa belladonna L
KR100792250B1 (en) 2006-06-09 2008-01-07 고려대학교 산학협력단 Novel gene of methyltransferase interacting with Cucumber mosaic virus 1a protein
CN102851288A (en) * 2012-06-30 2013-01-02 河南农业大学 PMT1 gene promoter for nicotine biosynthesis and application thereof
EP3113604A1 (en) * 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
US10405571B2 (en) 2015-06-26 2019-09-10 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
KR20210039421A (en) * 2018-07-26 2021-04-09 알트리아 클라이언트 서비시즈 엘엘씨 Compositions and methods based on PMT engineering for producing tobacco plants and products with altered alkaloid levels
US10897925B2 (en) 2018-07-27 2021-01-26 Joseph Pandolfino Articles and formulations for smoking products and vaporizers
US20200035118A1 (en) 2018-07-27 2020-01-30 Joseph Pandolfino Methods and products to facilitate smokers switching to a tobacco heating product or e-cigarettes
CN109321544A (en) * 2018-11-13 2019-02-12 中国烟草总公司郑州烟草研究院 The extracting method of putrescine N-methyltransferase in a kind of raising leaf tobacco extract

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US5260205A (en) * 1990-11-14 1993-11-09 Philip Morris Incorporated Method of purifying putrescine N-methyltransferase from tobacco plant extract with a polyamine
WO1993005646A1 (en) * 1991-09-13 1993-04-01 Technology Management Services, S.A. Reduction of nicotine levels in tobacco

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CN1127530A (en) 1996-07-24
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EP0701614A1 (en) 1996-03-20
JPH09502601A (en) 1997-03-18

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