CA2009917A1 - Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications - Google Patents

Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications

Info

Publication number
CA2009917A1
CA2009917A1 CA 2009917 CA2009917A CA2009917A1 CA 2009917 A1 CA2009917 A1 CA 2009917A1 CA 2009917 CA2009917 CA 2009917 CA 2009917 A CA2009917 A CA 2009917A CA 2009917 A1 CA2009917 A1 CA 2009917A1
Authority
CA
Canada
Prior art keywords
protein
cold shock
promoter
gene
agriculture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2009917
Other languages
French (fr)
Other versions
CA2009917C (en
Inventor
Joel Goldstein
N. Stephen Pollitt
Masayori Inouye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Medicine and Dentistry of New Jersey
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2009917A1 publication Critical patent/CA2009917A1/en
Application granted granted Critical
Publication of CA2009917C publication Critical patent/CA2009917C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Abstract

The major cold shock protein of E. coli, which comprises a nucleotide sequence encoding cs7.4 with functional equivalents thereof, along with a nucleic acid molecule having a promoter sequence which is capable of demonstrating transcription of a gene at a temperature below normal growth temperature of E. coli. Consequently, construct and DNA
sequence in which include the gene encoding cs7.4 protein, a promoter designed to drive cs7.4 protein, a promoter designed to drive cs7.4 expression, and the cspA
gene are disclosed.
Also, contemplated are transformed competent hosts and transgenic plants. The invention also provides for various applications and method in protein synthesis.
CA 2009917 1989-02-13 1990-02-13 Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications Expired - Lifetime CA2009917C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31033289A 1989-02-13 1989-02-13
US07/310,332 1989-02-13

Publications (2)

Publication Number Publication Date
CA2009917A1 true CA2009917A1 (en) 1990-08-13
CA2009917C CA2009917C (en) 2007-05-22

Family

ID=23202035

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2009917 Expired - Lifetime CA2009917C (en) 1989-02-13 1990-02-13 Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications

Country Status (4)

Country Link
EP (1) EP0423264A1 (en)
JP (1) JPH04500459A (en)
CA (1) CA2009917C (en)
WO (1) WO1990009447A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1914892A (en) * 1991-05-03 1992-12-21 Smithkline Beecham Corporation Low temperature-regulated promoters in e. coli
US5981280A (en) * 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
JP4057237B2 (en) * 1997-11-20 2008-03-05 タカラバイオ株式会社 Low temperature inducible expression vector
EP1803813A3 (en) * 2001-11-19 2007-11-14 Riken An environmental stress-responsive promoter and an gene encoding environmental stress-responsive transcriptional factor
EP2281895B1 (en) * 2003-09-29 2018-01-03 Monsanto Technology, LLC Methods for enhancing stress tolerance in plants and compositions thereof
CN1886514B (en) * 2003-09-29 2015-11-25 孟山都技术有限公司 For strengthening the method for stress tolerance in plants
JP5279339B2 (en) 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
CN110467655B (en) * 2019-08-14 2021-10-08 上海交通大学 Protein and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4464473A (en) * 1982-04-23 1984-08-07 The Regents Of The University Of California Ice nucleating microorganisms

Also Published As

Publication number Publication date
EP0423264A1 (en) 1991-04-24
CA2009917C (en) 2007-05-22
JPH04500459A (en) 1992-01-30
WO1990009447A1 (en) 1990-08-23

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Effective date: 20100213