CA2009917A1 - Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications - Google Patents

Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications

Info

Publication number
CA2009917A1
CA2009917A1 CA 2009917 CA2009917A CA2009917A1 CA 2009917 A1 CA2009917 A1 CA 2009917A1 CA 2009917 CA2009917 CA 2009917 CA 2009917 A CA2009917 A CA 2009917A CA 2009917 A1 CA2009917 A1 CA 2009917A1
Authority
CA
Canada
Prior art keywords
protein
cold shock
promoter
gene
agriculture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA 2009917
Other languages
French (fr)
Other versions
CA2009917C (en
Inventor
Joel Goldstein
N. Stephen Pollitt
Masayori Inouye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Medicine and Dentistry of New Jersey
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2009917A1 publication Critical patent/CA2009917A1/en
Application granted granted Critical
Publication of CA2009917C publication Critical patent/CA2009917C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The major cold shock protein of E. coli, which comprises a nucleotide sequence encoding cs7.4 with functional equivalents thereof, along with a nucleic acid molecule having a promoter sequence which is capable of demonstrating transcription of a gene at a temperature below normal growth temperature of E. coli. Consequently, construct and DNA
sequence in which include the gene encoding cs7.4 protein, a promoter designed to drive cs7.4 protein, a promoter designed to drive cs7.4 expression, and the cspA
gene are disclosed.
Also, contemplated are transformed competent hosts and transgenic plants. The invention also provides for various applications and method in protein synthesis.
CA002009917A 1989-02-13 1990-02-13 Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications Expired - Lifetime CA2009917C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31033289A 1989-02-13 1989-02-13
US07/310,332 1989-02-13

Publications (2)

Publication Number Publication Date
CA2009917A1 true CA2009917A1 (en) 1990-08-13
CA2009917C CA2009917C (en) 2007-05-22

Family

ID=23202035

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002009917A Expired - Lifetime CA2009917C (en) 1989-02-13 1990-02-13 Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications

Country Status (4)

Country Link
EP (1) EP0423264A1 (en)
JP (1) JPH04500459A (en)
CA (1) CA2009917C (en)
WO (1) WO1990009447A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019718A1 (en) * 1991-05-03 1992-11-12 Smithkline Beecham Corporation LOW TEMPERATURE-REGULATED PROMOTERS IN $i(E. COLI)
US5981280A (en) * 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
JP4057237B2 (en) 1997-11-20 2008-03-05 タカラバイオ株式会社 Low temperature inducible expression vector
CN100359012C (en) * 2001-11-19 2008-01-02 独立行政法人理化学研究所 Environmental stress-responsive promoters and genes encoding transcriptional factor
PL2281895T3 (en) 2003-09-29 2018-07-31 Monsanto Technology, Llc Methods for enhancing stress tolerance in plants and compositions thereof
SI1668141T1 (en) * 2003-09-29 2013-04-30 Monsanto Technology, Llc Methods for enhancing drought tolerance in plants and methods thereof
JP5279339B2 (en) 2008-05-16 2013-09-04 タカラバイオ株式会社 Composition for reverse transcription reaction
CN110467655B (en) * 2019-08-14 2021-10-08 上海交通大学 Protein and application thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4464473A (en) * 1982-04-23 1984-08-07 The Regents Of The University Of California Ice nucleating microorganisms

Also Published As

Publication number Publication date
CA2009917C (en) 2007-05-22
WO1990009447A1 (en) 1990-08-23
EP0423264A1 (en) 1991-04-24
JPH04500459A (en) 1992-01-30

Similar Documents

Publication Publication Date Title
Shimatake et al. Purified λ regulatory protein c II positively activates promoters for lysogenic development
US4582800A (en) Novel vectors and method for controlling interferon expression
US5710027A (en) Process and vector for expressing alpha-interferon in E. coli
Melcher A modular set of prokaryotic and eukaryotic expression vectors
US5580788A (en) Use of immunoglogulin-binding artificial proteins as molecular weight markers
WO1999029875A3 (en) Plant uridine diphosphate-glucose dehydrogenase genes, proteins, and uses thereof
FI90436C (en) Preparation of polypeptides having human gamma interferon-like activity
CA2066370A1 (en) Yield when disulfide-bonded proteins are secreted
CA2009917A1 (en) Cold shock protein, gene coding therefor, promoter for gene encoding cold shock and other proteins, methods and uses as antifreeze protein in agriculture and other applications
Li et al. Optimized gene synthesis and high expression of human interleukin-18
ATE208815T1 (en) GENETIC IGFBP-5 RODING MATERIAL
Nanbu et al. Purification of an AU-rich RNA binding protein from Sarcophaga peregrina (flesh fly) and its identification as a Thiolase
US5629205A (en) Promoters for gene expression
KR870010193A (en) Human Pancreatic Elastase I
Windass et al. The construction of a synthetic Escherichia coli trp promoter and its use in the expression of a synthetic interferon gene
DE69131979D1 (en) GENETIC IGFBP-4 RODING MATERIAL
EP0622460B1 (en) Plasmid and escherichia coli transformed with it
KR930701603A (en) Recombinant 47 and 31 Kilodalton Cocoa Protein and Its Precursors
WO1995008628A3 (en) Nucleic acids encoding merosin, merosin fragments and uses thereof
WO2003048325A3 (en) Nucleotide sequences of shrimp beta-actin and actin promoters and their use in genetic transformation technology
Ganesa et al. Purification and molecular properties of the toxin coded by Ustilagomaydis virus P4
KR900700615A (en) Method for preparing human neutrophil chemotactic factor polypeptide
Sakiyama et al. Relationship between molecular weight of T4 phage-induced deoxynucleotide kinase and the size of its messenger ribonucleic acid
Hara et al. Purification and kinetic properties of phenoloxidase from pupae of the housefly
JP3709422B2 (en) Regulators involved in nitrilase gene expression and genes thereof

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry
MKEX Expiry

Effective date: 20100213