KR960041356A - Method for preparing lactic acid bacteria raw material using Lactobacillus asidophilus strain (KCTC 3145) - Google Patents

Method for preparing lactic acid bacteria raw material using Lactobacillus asidophilus strain (KCTC 3145) Download PDF

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Publication number
KR960041356A
KR960041356A KR1019950014279A KR19950014279A KR960041356A KR 960041356 A KR960041356 A KR 960041356A KR 1019950014279 A KR1019950014279 A KR 1019950014279A KR 19950014279 A KR19950014279 A KR 19950014279A KR 960041356 A KR960041356 A KR 960041356A
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South Korea
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kctc
strain
lactic acid
lactobacillus asidophilus
acid bacteria
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KR1019950014279A
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Korean (ko)
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KR0148340B1 (en
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차성관
홍석산
김왕준
구영조
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서중일
한국식품개발연구원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/04—Preserving or maintaining viable microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 공시균주로부터 선별된, 우유배지에서의 증식이나 혐기조건에서의 성장 및 내산성, 내담즙성이 우수하며, 베타-갈락토시데이즈(β-galactosidase) 활성이 높은 락토바실루스 아시도필루스(KCTC 3145) 균주와, 전기 균주의 배양을 위한 배지 및 전기 균주를 이용하여 경제적으로 유산균 원말을 제조한는 방법을 제공한다. 본 발명의 락토바실루스 아시도필루스 균주는 프로바이오텍스에 적합하며, 본 발명의 제조방법을 이용하면 경제적으로 균체를 생산할 수 있고, 균체를 함유하는 제품중에서의 균체 생존률을 높이는 효과를 얻을 수 있다. 따라서, 본 발명은 프로바이오틱스의 제조에 이용될 수 있을 것이다.The present invention is Lactobacillus asidophilus, which is selected from the test strains, has excellent growth, acid resistance, and bile resistance in milk medium, anaerobic conditions, and high beta-galactosidase activity. (KCTC 3145) Provides a method for producing lactic acid bacteria raw material economically using the strain, the medium for culturing the electric strain and the electric strain. The Lactobacillus asidophilus strain of the present invention is suitable for probiotex, and by using the production method of the present invention, it is possible to economically produce cells, and increase the viability of cells in products containing the cells. . Thus, the present invention may be used to prepare probiotics.

Description

락토바실루스 아시도필루스 균주(KCTC 3145)를 이용한 유산균 원말의 제조방법Method for preparing lactic acid bacteria raw material using Lactobacillus asidophilus strain (KCTC 3145)

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (3)

락토바실루스 아시도필루스(KCTC 3145) 균주를 포함하는 프로바이오텍스에 적합한 유산균 원말.A lactic acid bacterium suitable for probiotics, including Lactobacillus asidophilus (KCTC 3145) strains. 유청분말(whey powder) 1 내지 10%(w/v), 싸이오톤(Thiotone) 0.1 내지 1.0%(w/v), 트윈(Tween) 80 0.01 내지 0.1%(w/v) 및 탄산칼슘 0.1 내지 0.5%(w/v)를 함유하는 락토바실루스 아시도필루스(KCTC 3145) 균주를 위한 배양배지.Whey powder 1 to 10% (w / v), Thytone 0.1 to 1.0% (w / v), Tween 80 0.01 to 0.1% (w / v) and calcium carbonate 0.1 to Culture medium for Lactobacillus asidophilus (KCTC 3145) strain containing 0.5% (w / v). 제2항이 배지에 락토바실루스 아시도필루스(KCTC 3145)를 배양하여 균체를 수확한 다음, 동결건조 중의 생존률을 높이기 위해 탄산나트륨을 0.01 내지 0.1%(w/v) 첨가하는 것을 특징으로 하는 유산균 원말의 제조방법.Claim 2 is cultured Lactobacillus asidophilus (KCTC 3145) in the medium to harvest the cells, and then lactic acid bacteria characterized in that the addition of sodium carbonate 0.01 to 0.1% (w / v) to increase the survival rate during lyophilization Manufacturing method. ※ 참고사항 : 최초출원 내용에 의하여 공개히는 것임.※ Note: It is to be disclosed based on the initial application.
KR1019950014279A 1995-05-31 1995-05-31 Method of antibiotic bacteriocin using lactobacillus acidophilus KR0148340B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950014279A KR0148340B1 (en) 1995-05-31 1995-05-31 Method of antibiotic bacteriocin using lactobacillus acidophilus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950014279A KR0148340B1 (en) 1995-05-31 1995-05-31 Method of antibiotic bacteriocin using lactobacillus acidophilus

Publications (2)

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KR960041356A true KR960041356A (en) 1996-12-19
KR0148340B1 KR0148340B1 (en) 1998-10-15

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030097235A (en) * 2002-06-20 2003-12-31 (주) 피엘바이오 Medium for lactic acid producing bacteria not affecting taste, color and flavour of product

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