KR960034425A - Secretory Expression Vectors for Animal Cells - Google Patents

Secretory Expression Vectors for Animal Cells Download PDF

Info

Publication number
KR960034425A
KR960034425A KR1019950005423A KR19950005423A KR960034425A KR 960034425 A KR960034425 A KR 960034425A KR 1019950005423 A KR1019950005423 A KR 1019950005423A KR 19950005423 A KR19950005423 A KR 19950005423A KR 960034425 A KR960034425 A KR 960034425A
Authority
KR
South Korea
Prior art keywords
animal cells
foreign protein
secretory expression
expression vectors
restriction enzyme
Prior art date
Application number
KR1019950005423A
Other languages
Korean (ko)
Other versions
KR0143597B1 (en
Inventor
김창환
이태호
Original Assignee
성재갑
주식회사 Lg 화학
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 성재갑, 주식회사 Lg 화학 filed Critical 성재갑
Priority to KR1019950005423A priority Critical patent/KR0143597B1/en
Publication of KR960034425A publication Critical patent/KR960034425A/en
Application granted granted Critical
Publication of KR0143597B1 publication Critical patent/KR0143597B1/en

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 인간 사이토메갈로바이러스(cytomegalovirus, CMV)프로모터, 분비를 유도하는 인간 적혈구 형성 자극인자(erythropotetin, EPO)의 시그널 서열 및 외래 단백질을 클로닝할 수 있는 제한효소 인식 서열을 포함하는, 높은 발현율과 분비율을 갖는 동물 세포용 분비형 발현 벡터에 관한 것이다.The present invention provides a high expression rate including a human cytomegalovirus (CMV) promoter, a signal sequence of human erythropotetin (EPO) that induces secretion, and a restriction enzyme recognition sequence capable of cloning foreign proteins. A secretory expression vector for animal cells having a secretion rate.

Description

동물세포용 분비형 발현 벡터Secretory Expression Vectors for Animal Cells

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 발현벡터인 pMI-003의 제작과정과 그 구조를 나타낸 것이고, 제2도는 pMI-003에 의해 형질전환된 동물세포로부터 생산되어 세포외로 분비된 인간 면역 글로불린 G의 Fc 부분을 웨스턴 분석으로 확인한 결과이다.Figure 1 shows the fabrication process and its structure of the expression vector pMI-003, Figure 2 shows the Fc portion of human immunoglobulin G produced from animal cells transformed by pMI-003 and secreted by Western analysis. The result is confirmed.

Claims (3)

인간 사이토메갈로 바이러스(cytomegalovirus) 프로모터, 인간 적혈구 형성 자극인자(erythropoietin)의 시그널 서열 및 외래 단백질 유전자를 클로닝할 수 있는 제한효소 인식 서열을 포함하는 동물세포용 분비형 발현 벡터.A secretory expression vector for animal cells comprising a human cytomegalovirus promoter, a signal sequence of human erythropoietin, and a restriction enzyme recognition sequence capable of cloning foreign protein genes. 제1항에 있어서, 발현벡터 pMI-003(KCTC 0152BP).The expression vector pMI-003 (KCTC 0152BP) according to claim 1. 제1항 또는 제2항의 벡터에 포함된 제한효소 인식서열을 이용하여 상기 시그널 서열 아래에 상기 외래 단백질 유전자를 해독 프레임 맞게 연결한 후 동물세포에서 발현시켜 분비된 상기 외래 단백질을 회수함을 포함하는, 상기 외래 단백질의 제조 방법.Using the restriction enzyme recognition sequence included in the vector of claim 1 or 2 to connect the foreign protein gene under the signal sequence to fit the frame of translation, and then expressing in the animal cell to recover the secreted foreign protein. , Manufacturing method of the foreign protein. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950005423A 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells KR0143597B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950005423A KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950005423A KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

Publications (2)

Publication Number Publication Date
KR960034425A true KR960034425A (en) 1996-10-22
KR0143597B1 KR0143597B1 (en) 1998-07-01

Family

ID=66548998

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950005423A KR0143597B1 (en) 1995-03-16 1995-03-16 Secretory Expression Vectors for Animal Cells

Country Status (1)

Country Link
KR (1) KR0143597B1 (en)

Also Published As

Publication number Publication date
KR0143597B1 (en) 1998-07-01

Similar Documents

Publication Publication Date Title
DE69534185D1 (en) NON-TOXIC, NON-TOXIGEN, NON-PATHOGENIC FUSARIUM EXPRESSION SYSTEM, AND PROMOTERS AND TERMINATORS TO BE USED IN IT
DE3484664D1 (en) RECOMBINANT IMMUNOGLOBULIN PREPARATIONS, METHOD FOR THE PRODUCTION THEREOF, DNA SEQUENCES, EXPRESSION VECTORS AND RECOMBINANT ACTIVE CELLS FOR THIS.
CA2087261A1 (en) Purification directed cloning of peptides
CA2086936A1 (en) Method for producing members of specific binding pairs
DK97784D0 (en) DNA segment
EP1798239A3 (en) G-protein coupled receptors and uses therefor
ATE144281T1 (en) YEAST CELLS OF THE GENUS SCHWANNIOMYCES
DK1090129T4 (en) Overexpression of phytase genes in yeast systems
ATA192895A (en) PRODUCTION OF PROTEINS FROM PRO-PROTEINS BY FUSION PROTEINS DERIVED FROM FURIN OR FURINANALOGS
DE69426289T2 (en) Signal sequences for the secretion of heterologous proteins from yeast
DK0443874T3 (en) Vectors and Compounds for Expression of Glycosylation Mutants of Human Protein C
KR960034425A (en) Secretory Expression Vectors for Animal Cells
EP0255320A3 (en) Production of proteins in myeloma cells
DE3880473T2 (en) EXPRESSION VECTORS FOR PROTEIN SYNTHESIS AND PLASMID REPLICONS AND SEQUENCE CASSETTES SUITABLE FOR THE PRODUCTION OF SUCH VECTORS.
ZA949981B (en) Methods for producing protein C
SE9504019D0 (en) Method for production of proteins
FI951633A0 (en) Increased production of secretory proteins by eukaryotic recombinant cells
DE3877136D1 (en) RECOMBINANT HISTIDINE PROTEIN OF PLASMODIUM FALCIPARUM, ITS PRODUCTION AND USE.
DK0725822T3 (en) Method for Recombinant Production of Proteins in Yeast
DK0870050T3 (en) Virus expressing a toxic protein and producer cell lines
DE69533840D1 (en) IMPROVED EXPRESSION VECTOR FOR THE PRODUCTION OF RECOMBINANT PROTEINS
KR950023720A (en) Expression Vectors of the Human Midkine Gene and Its Expression in Yeast

Legal Events

Date Code Title Description
A201 Request for examination
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20130327

Year of fee payment: 16

FPAY Annual fee payment

Payment date: 20140311

Year of fee payment: 17

EXPY Expiration of term