KR960034425A - Secretory Expression Vectors for Animal Cells - Google Patents
Secretory Expression Vectors for Animal Cells Download PDFInfo
- Publication number
- KR960034425A KR960034425A KR1019950005423A KR19950005423A KR960034425A KR 960034425 A KR960034425 A KR 960034425A KR 1019950005423 A KR1019950005423 A KR 1019950005423A KR 19950005423 A KR19950005423 A KR 19950005423A KR 960034425 A KR960034425 A KR 960034425A
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- KR
- South Korea
- Prior art keywords
- animal cells
- foreign protein
- secretory expression
- expression vectors
- restriction enzyme
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 인간 사이토메갈로바이러스(cytomegalovirus, CMV)프로모터, 분비를 유도하는 인간 적혈구 형성 자극인자(erythropotetin, EPO)의 시그널 서열 및 외래 단백질을 클로닝할 수 있는 제한효소 인식 서열을 포함하는, 높은 발현율과 분비율을 갖는 동물 세포용 분비형 발현 벡터에 관한 것이다.The present invention provides a high expression rate including a human cytomegalovirus (CMV) promoter, a signal sequence of human erythropotetin (EPO) that induces secretion, and a restriction enzyme recognition sequence capable of cloning foreign proteins. A secretory expression vector for animal cells having a secretion rate.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 발현벡터인 pMI-003의 제작과정과 그 구조를 나타낸 것이고, 제2도는 pMI-003에 의해 형질전환된 동물세포로부터 생산되어 세포외로 분비된 인간 면역 글로불린 G의 Fc 부분을 웨스턴 분석으로 확인한 결과이다.Figure 1 shows the fabrication process and its structure of the expression vector pMI-003, Figure 2 shows the Fc portion of human immunoglobulin G produced from animal cells transformed by pMI-003 and secreted by Western analysis. The result is confirmed.
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950005423A KR0143597B1 (en) | 1995-03-16 | 1995-03-16 | Secretory Expression Vectors for Animal Cells |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950005423A KR0143597B1 (en) | 1995-03-16 | 1995-03-16 | Secretory Expression Vectors for Animal Cells |
Publications (2)
Publication Number | Publication Date |
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KR960034425A true KR960034425A (en) | 1996-10-22 |
KR0143597B1 KR0143597B1 (en) | 1998-07-01 |
Family
ID=66548998
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019950005423A KR0143597B1 (en) | 1995-03-16 | 1995-03-16 | Secretory Expression Vectors for Animal Cells |
Country Status (1)
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KR (1) | KR0143597B1 (en) |
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1995
- 1995-03-16 KR KR1019950005423A patent/KR0143597B1/en not_active IP Right Cessation
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Publication number | Publication date |
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KR0143597B1 (en) | 1998-07-01 |
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