KR960034402A - Streptokinase-expressing microorganisms and preparation method of streptokinase using the same - Google Patents

Streptokinase-expressing microorganisms and preparation method of streptokinase using the same Download PDF

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Publication number
KR960034402A
KR960034402A KR1019950007251A KR19950007251A KR960034402A KR 960034402 A KR960034402 A KR 960034402A KR 1019950007251 A KR1019950007251 A KR 1019950007251A KR 19950007251 A KR19950007251 A KR 19950007251A KR 960034402 A KR960034402 A KR 960034402A
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KR
South Korea
Prior art keywords
streptokinase
psk100
expression vector
recombinant microorganism
escherichia coli
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Application number
KR1019950007251A
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Korean (ko)
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KR0134997B1 (en
Inventor
변시명
배광희
이시형
김일철
박도근
Original Assignee
심상철
한국과학기술원
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Priority to KR1019950007251A priority Critical patent/KR0134997B1/en
Publication of KR960034402A publication Critical patent/KR960034402A/en
Application granted granted Critical
Publication of KR0134997B1 publication Critical patent/KR0134997B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24029—Aureolysin (3.4.24.29)

Abstract

본 발명은 스트렙토키나제를 발현하는 미생물 및 그를 이용한 스트렙토키나제의 제조방법에 관한 것이다. 좀 더 구체적으로, 본 발명은 스트렙토키나제 발현벡터와 그것으로 형질전환된 재조합 미생물 및 재조합 미생물로부터 스트렙토키나제를 제조하는 방법에 관한 것이다. 본 발명의 스트렙토키나제 발현벡터 pSK100으로 형질전환된 재조합 미생물을 배양하는 경우, 임상적으로 안전한 고순도의 스트렙토키나제를 경제적으로 대량 생산하는 효과를 가진다.The present invention relates to a microorganism expressing streptokinase and a method for producing streptokinase using the same. More specifically, the present invention relates to a streptokinase expression vector and a recombinant microorganism transformed therewith and a method for producing streptokinase from recombinant microorganisms. When culturing the recombinant microorganism transformed with the streptokinase expression vector pSK100 of the present invention, it has the effect of economically mass production of clinically safe high purity streptokinase.

Description

스트렙토키나제 발현 미생물 및 그를 이용한 스트렙토키나제의 제조방법Streptokinase-expressing microorganisms and preparation method of streptokinase using the same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 스트렙토키나제 발현벡터 pSK100의 구조를 나타내는 그림이다, 제2도는 pSK100 벡터를 제조하는 공정을 도식적으로 나타낸 그림이다, 제3도는 pSK100 벡터로 형질전환된 재조합 대장균 JM109의 배양시간에 따른 스트렙토키나제 발현 정도를 나타내는 그래프이다.Figure 1 is a diagram showing the structure of the streptokinase expression vector pSK100, Figure 2 is a diagram showing the process of preparing the pSK100 vector, Figure 3 is a streptokinase according to the culture time of recombinant E. coli JM109 transformed with the pSK100 vector It is a graph showing the degree of expression.

Claims (4)

tac 전사촉진제, ompA 신호서열 및 스트렙토키나제 유전자를 포함하는 스트렙토키나제 발현벡터 pSK100.Streptokinase expression vector pSK100 comprising a tac promoter, ompA signal sequence and streptokinase gene. 제1항의 스트렙토키나제 발현벡터 pSK100에 의하여 형질전환 되어 스트렙토키나제를 생산하는 재조합 미생물 대장균 JM109/pSK100(Escherichia coli JM109/pSK100) (KCTC 8653P).Recombinant microorganism Escherichia coli JM109 / pSK100 (Escherichia coli JM109 / pSK100) transformed with the streptokinase expression vector pSK100 of claim 1 to produce streptokinase (KCTC 8653P). 제2항의 재조합 미생물 대장균 JM109/pSK100(Escherichia coli JM109/pSK100)을 배양하여 수득하는 공정을 포함하는 고순도의 스트렙토키나제 제조방법.A method for producing high purity streptokinase comprising the step of culturing the recombinant microorganism Escherichia coli JM109 / pSK100 (Escherichia coli JM109 / pSK100) of claim 2. 제1항의 스트렙토키나제 발현벡터 pSK100로 형질전환된 재조합 미생물을 배양하여 수득하는 공정에 의해 제조된 스트렙토키나제.A streptokinase prepared by a process obtained by culturing a recombinant microorganism transformed with the streptokinase expression vector pSK100 of claim 1. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950007251A 1995-03-31 1995-03-31 Streptokinase producing escherichia coli and preparation method of streptokinase KR0134997B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950007251A KR0134997B1 (en) 1995-03-31 1995-03-31 Streptokinase producing escherichia coli and preparation method of streptokinase

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Application Number Priority Date Filing Date Title
KR1019950007251A KR0134997B1 (en) 1995-03-31 1995-03-31 Streptokinase producing escherichia coli and preparation method of streptokinase

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KR960034402A true KR960034402A (en) 1996-10-22
KR0134997B1 KR0134997B1 (en) 1998-04-20

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