KR960031619A - Production method of polysaccharides by liquid culture of Ganoderma lucidum - Google Patents

Production method of polysaccharides by liquid culture of Ganoderma lucidum Download PDF

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Publication number
KR960031619A
KR960031619A KR1019950003283A KR19950003283A KR960031619A KR 960031619 A KR960031619 A KR 960031619A KR 1019950003283 A KR1019950003283 A KR 1019950003283A KR 19950003283 A KR19950003283 A KR 19950003283A KR 960031619 A KR960031619 A KR 960031619A
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South Korea
Prior art keywords
ganoderma lucidum
glucan
liquid culture
medium
polysaccharides
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KR1019950003283A
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Korean (ko)
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KR0156986B1 (en
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강태수
이신영
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이신영
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Publication of KR0156986B1 publication Critical patent/KR0156986B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/042—Gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Epidemiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Birds (AREA)
  • Inorganic Chemistry (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 영지버섯을 최적의 조건하에 액체배양하여 β-1,3-글루칸을 생산하는 방법에 관한 것이고, 이 방법은 영지버섯의 균사체를 C/N비가 7~9.5 : 1의 범위에 있고, pH 4.0~6.0, 온도 20~40℃, 교반속도 80~140rpm인 조건하에 액체배양하여 β-1,3-글루칸을 배지중에 축적시키고, 배양액으로부터 상기 β-1,3-글루칸을 수확하는 단계를 포함함을 특징으로 한다.The present invention relates to a method for producing β-1,3-glucan by liquid culture of Ganoderma lucidum under optimal conditions, and this method has a C / N ratio of Ganoderma lucidum mycelium in the range of 7-9.5: 1, Incubating the liquid under the conditions of pH 4.0 ~ 6.0, temperature 20 ~ 40 ℃, stirring speed 80 ~ 140rpm to accumulate β-1,3-glucan in the medium, and harvesting the β-1,3-glucan from the culture medium It is characterized by including.

영지버섯으로부터 생산된 β-1,3-글루칸은 우수한 항암 활성을 가질 뿐말 아니라 우수한 겔화능을 갖고있어, 식품 또는 의약품 등에 첨가하여 항암활성을 갖는 겔화 식품 또는 의약품을 제조하는데 유용하게 사용될 수 있다.Β-1,3-glucan produced from Ganoderma lucidum not only has excellent anticancer activity, but also has excellent gelation ability, and thus can be usefully used for preparing gelated foods or medicines having anticancer activity in addition to foods or pharmaceuticals.

Description

영지 버섯의 액체 배양에 의한 다당류의 생산 방법Production method of polysaccharides by liquid culture of Ganoderma lucidum

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (4)

영지 버섯을 액체 배양하여 항암활성을 갖는 다당류를 생산하는 방법에 있어서, 상기 방법은 영지버섯의 균사체를 C/N비가 7~9.5 : 1의 범위에 있고, pH 4.0~6.0, 온도 20~40℃, 교반속도 80~140rpm인 조건하에 액체배양하여 β-1,3-글루칸을 배지중에 축적시키고, 배양액으로부터 상기 β-1,3-글루칸을 수확하는 단계를 포함함을 특징으로 하는 영지버섯 균사체의 액체배양에 의한 β-1,3-글루칸의 생산방법.In the method of producing a polysaccharide having anticancer activity by liquid culture of Ganoderma lucidum mushroom, the method has a C / N ratio of Ganoderma lucidum mycelium in the range of 7 ~ 9.5: 1, pH 4.0 ~ 6.0, temperature 20 ~ 40 ℃ The liquid culture under the condition of agitation speed of 80 ~ 140rpm to accumulate β-1,3-glucan in the medium, and harvesting the β-1,3-glucan from the culture medium of the Ganoderma lucidum mycelium, characterized in that Method for producing β-1,3-glucan by liquid culture. 제1항에 있어서, C/N비는 약 8.3 : 1임을 특징으로 하는 방법.The method of claim 1 wherein the C / N ratio is about 8.3: 1. 제1항에 있어서, 액체 배양을 위한 배지는 글루코스 3~10%(w/v), 효모추출액 0.1~1.0%(w/v), (NH4)2HPO40.05~0.3%(w/v) 및 KH2PO40.01~0.2%(w/v)를 함유함을 특징으로 하는 방법.The medium for culturing liquid according to claim 1, wherein the medium for liquid culture is glucose 3-10% (w / v), yeast extract 0.1-1.0% (w / v), (NH 4 ) 2 HPO 4 0.05-0.3% (w / v). ) And KH 2 PO 4 0.01 to 0.2% (w / v). 제1항의 방법에 의해 얻은 β-1,3-글루칸을 유효성분으로 함유하는 겔화제 조성물.A gelling agent composition comprising β-1,3-glucan obtained by the method of claim 1 as an active ingredient. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950003283A 1995-02-20 1995-02-20 Preparation process of polysaccharide by cultivating ganoderma lucidum KR0156986B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950003283A KR0156986B1 (en) 1995-02-20 1995-02-20 Preparation process of polysaccharide by cultivating ganoderma lucidum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950003283A KR0156986B1 (en) 1995-02-20 1995-02-20 Preparation process of polysaccharide by cultivating ganoderma lucidum

Publications (2)

Publication Number Publication Date
KR960031619A true KR960031619A (en) 1996-09-17
KR0156986B1 KR0156986B1 (en) 1998-10-15

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004082691A1 (en) * 2003-03-18 2004-09-30 Bioprogen Co. Ltd. Composition comprising soluble glucan oligomer from saccharomyces cerevisiae is2 for immune activation or prevention and treatment of cancer and the preparation method thereof
KR100470734B1 (en) * 2002-06-19 2005-02-21 주식회사 티지 바이오텍 Method for preparation of exo-polymers from submerged mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) and the use thereof
KR100773126B1 (en) * 2006-08-25 2007-11-02 조선대학교산학협력단 Medium for mycelial growth using fomitopsis pinicola
KR20100083020A (en) * 2009-01-12 2010-07-21 순천향대학교 산학협력단 Feed additives containing betaglucan and preparation method of the same
CN112458129A (en) * 2020-12-29 2021-03-09 青岛润达生物科技有限公司 Preparation process of short-chain ganoderan sulfuric acid derivative

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100383078B1 (en) * 2000-06-16 2003-05-12 환인제약 주식회사 Process for mass production of Phellinus linteus by fed-batch culture
KR20030030636A (en) * 2001-10-12 2003-04-18 주식회사 싸이제닉 Manufacturing method of highly active antitumor protein-polysaccharides using fermentation of the fruit bodies of mushroom
KR100468648B1 (en) * 2003-01-29 2005-01-27 학교법인 영광학원 Ganoderma lucidum exo- and endo-biopolymer having a hypolipidemic effect and the use thereof

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100470734B1 (en) * 2002-06-19 2005-02-21 주식회사 티지 바이오텍 Method for preparation of exo-polymers from submerged mycelial culture of Ganoderma lucidum TG(KCTC 10241BP) and the use thereof
WO2004082691A1 (en) * 2003-03-18 2004-09-30 Bioprogen Co. Ltd. Composition comprising soluble glucan oligomer from saccharomyces cerevisiae is2 for immune activation or prevention and treatment of cancer and the preparation method thereof
KR100773126B1 (en) * 2006-08-25 2007-11-02 조선대학교산학협력단 Medium for mycelial growth using fomitopsis pinicola
KR20100083020A (en) * 2009-01-12 2010-07-21 순천향대학교 산학협력단 Feed additives containing betaglucan and preparation method of the same
CN112458129A (en) * 2020-12-29 2021-03-09 青岛润达生物科技有限公司 Preparation process of short-chain ganoderan sulfuric acid derivative

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Publication number Publication date
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