KR960028910A - Method for preparing hydrolyzate of Zara enzyme mainly containing low molecular weight peptide and health nutrition composition using same - Google Patents

Method for preparing hydrolyzate of Zara enzyme mainly containing low molecular weight peptide and health nutrition composition using same Download PDF

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Publication number
KR960028910A
KR960028910A KR1019950001311A KR19950001311A KR960028910A KR 960028910 A KR960028910 A KR 960028910A KR 1019950001311 A KR1019950001311 A KR 1019950001311A KR 19950001311 A KR19950001311 A KR 19950001311A KR 960028910 A KR960028910 A KR 960028910A
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South Korea
Prior art keywords
molecular weight
powder
enzyme
low molecular
hydrolyzate
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KR1019950001311A
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Korean (ko)
Inventor
방성모
이병순
권영석
임달택
노경원
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한동근
주식회사 태평양
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Priority to KR1019950001311A priority Critical patent/KR960028910A/en
Publication of KR960028910A publication Critical patent/KR960028910A/en

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  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

본발명은 자라 분말의 단백질 효소분해물을 제조하는 방법 및 이 방법에 의해 얻어진 효소분해물을 유효성분으로 함유하는 건강영양조성물을 제공한다.The present invention provides a method for producing a protein enzymatic digest of a powder and a health nutrition composition containing the enzymatic digest obtained by the method as an active ingredient.

본 발명의 방법은 자라분말을 이온교환 수중에 10~30W/V%가 되도록 분산시키는 공정; 분산액을 트립신 또는 미생물 유래의 알카리성 프로테아제로 처리하여 효소 가수분해물을 얻어 분자량 300~1500의 저분자량 펩타이드가 주로 생성되도록 하는 공정; 및 효소 가수분해물을 가열하여 효소를 실활시키는 공정을 포함함을 특징으로 하며, 이 방법에 의해 면역중강 효과가 있는 분자량 300~1500의 저분자량 펩타이드를 주고 함유하는 자라 효소 가수분해물을 얻을 수 있다.The method of the present invention comprises the steps of dispersing the growth powder to 10 ~ 30W / V% in ion-exchanged water; Treating the dispersion with an trypsin or an alkaline protease derived from a microorganism to obtain an enzymatic hydrolyzate so that a low molecular weight peptide having a molecular weight of 300-1500 is mainly produced; And it is characterized in that it comprises a step of heating the enzyme hydrolyzate to deactivate the enzyme, by this method can be obtained to give the enzyme hydrolyzate to give a low molecular weight peptide having a molecular weight of 300 ~ 1500 having an immune gravitation effect.

본 발명에 따른 건강영양조성물은 상기 방법에 의해 얻은 면역중강 효과가 있는 분자량 300~1500의 저분자량 펩타이드를 주로 함유하는 자라 효소 가수분해물을 유효성분으로 함유하며 기타 건강보조성분을 함유한다.The health nutrient composition according to the present invention contains an enzyme hydrolyzate that grows mainly containing a low molecular weight peptide having a molecular weight of 300-1500 obtained by the above method as an active ingredient, and contains other health supplement components.

Description

저분자량 펩타이드를 주로 함유한 자라 효소 가수분해물의 제조방법 및 이를 이용한 건강영양 조성물Method for preparing hydrolyzate of Zara enzyme mainly containing low molecular weight peptide and health nutrition composition using same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (7)

자라분말을 이온교환 수중에 10~30W/V%가 되도록 분산시키는 공정; 분산액을 트립신 또는 미생물 유래의 알카리성 프로테아제로 처리하여 효소 가수분해물을 얻어 분자량 300~1500의 저분자량 펩타이드가 주로 생성되도록 하는 공정; 효소 가수분해물을 가열하여 효소를 실활시키는 공정을 포함함을 특징으로 하는 면역중강 효과가 있는 분자량 300~1500의 저분자량 펩타이드를 주로 함유하는 자라 효소 가수분해물의 제조방법.Dispersing the Zara powder so as to be 10-30 W / V% in ion-exchanged water; Treating the dispersion with an trypsin or an alkaline protease derived from a microorganism to obtain an enzymatic hydrolyzate so that a low molecular weight peptide having a molecular weight of 300-1500 is mainly produced; A method for producing an enzyme hydrolyzate, comprising a low molecular weight peptide having a molecular weight of 300-1500 having an immune gravitation effect, comprising the step of heating the enzyme hydrolyzate to deactivate the enzyme. 제1항에 있어서, 트립신을 이용한 효소분해는 기질액의 pH를 7~9로 하고, 기질인 자라분말 1g당 효소 1,000~50,000unit가 되도록 첨가하여 35~55℃의 온도에서 1~6시간 실시하는 것을 특징으로 하는 제조방법.According to claim 1, enzyme digestion using trypsin is carried out for 1 to 6 hours at a temperature of 35 ~ 55 ℃ by adding so that the pH of the substrate solution to 7 ~ 9, 1,000 ~ 50,000 units of enzyme per 1 g of Jara powder as a substrate Manufacturing method characterized in that. 제1항에 있어서, 미생물 유래의 알카리성 프로테아제의 경우 효소분해는 기질액의 pH를 8~10으로 하고, 기질인 자라분말 1g당 효소 1,000~50,000unit가 되도록 첨가하여 35~55℃의 온도에서 1~6시간 실시하는 것을 특징으로 하는 제조방법.According to claim 1, In the case of alkaline protease derived from microorganisms, the enzyme decomposition is the pH of the substrate solution to 8 ~ 10, and added so that the enzyme 1,000 ~ 50,000 units per 1g of Jara powder as a substrate at a temperature of 35 ~ 55 ℃ A manufacturing method characterized by carrying out ~ 6 hours. 제1항 또는 제3항에 있어서, 미생물 유래의 알카리성 프로테아제는 세균 유래의 프로테아제 또는 사상균 유래의 프로테아제임을 특징으로 하는 제조방법.The method according to claim 1 or 3, wherein the alkaline protease derived from the microorganism is a protease derived from bacteria or a protease derived from filamentous fungi. 자라 분말을 트립신 또는 미생물 유래의 알카리성 프로테아제로 처리하여 얻은 면역중강효과가 있는 분자량 300~1500의 저분자량 펩타이드를 주로 함유한 자라 효소 가수분해물을 유효 성분으로 하고 여기에 기타 건강보조성분 등을 배합하여 이루어진 것임을 특징으로 하는 건강영양 조성물.Zara powder is prepared by treatment with trypsin or alkaline protease derived from microorganisms. It is an active ingredient of Zara enzyme hydrolyzate containing mainly low molecular weight peptide with molecular weight of 300 ~ 1500. Health nutrition composition, characterized in that consisting of. 제5항에 있어서, 건강보조성분은 비타민A, 베타카로틴, 비타민B1, 비타민B2, 비타민B3, 비타민C, 천연토코페롤, 정제어유, 마늘유, 월견초유, 대두유, 포도씨유, 옥배유, 스쿠알렌. 소맥배야유, 콘도로이친함유분말(소연골추출분말, 상어연골추출물분말) 마늘분말, 로얄제리, 클로렐라, 우골분, 알로에, 천연타우린, 옥사코사놀, 화분, 녹혈, 녹용, 봉밀, 레시친, 인삼, 당귀, 구기자, 영지, 운지, 표고버섯, 작약, 지황, 알콕시글리세롤, 엘-트립토판, 엘-페닐알라닌, 엘-트레오닌, 엘-이소루이신, 엘-아스파라긴산 또는 엘-알라닌의 1종 또는 2종 이상의 혼합물임을 특징으로 하는 건강영양 조성물.According to claim 5, the health supplement ingredients vitamin A, beta-carotene, vitamin B 1 , vitamin B 2 , vitamin B 3 , vitamin C, natural tocopherol, refined fish oil, garlic oil, walnut colostrum oil, soybean oil, grape seed oil, jade oil, Squalene. Wheat pear oil, Condo chin powder (bovine cartilage extract powder, shark cartilage extract powder) Garlic powder, royal jelly, chlorella, beef bone powder, aloe, natural taurine, oxacosanol, pollen, green blood, deer antler, beeswax, lecithin, One or two of ginseng, Angelica, wolfberry, ganoderma lucidum, fingering, shiitake, peony, turmeric, alkoxyglycerol, el-tryptophan, el-phenylalanine, el-threonine, el-isorucin, el-aspartic acid or el-alanine Health nutrition composition characterized in that the mixture of more than one species. 제5항에 있어서, 건강영양 조성물은 연질캡슐, 경질캡슐, 과립, 정제, 분말, 환제제 또는 음료의 제형을 가짐을 특징으로 하는 건강영양 조성물.The health nutrition composition of claim 5, wherein the health nutrition composition has a formulation of soft capsule, hard capsule, granule, tablet, powder, pill or beverage. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950001311A 1995-01-25 1995-01-25 Method for preparing hydrolyzate of Zara enzyme mainly containing low molecular weight peptide and health nutrition composition using same KR960028910A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010025612A (en) * 2001-01-11 2001-04-06 조석형 Method for preparing a peptide and low molecular weight peptides
KR100475833B1 (en) * 1998-01-24 2005-12-26 학교법인 호서학원 High yield peptide preparation method
KR100566938B1 (en) * 2005-05-14 2006-04-03 한국식품연구원 Process for preparing crude peptide from amyda japonica
KR101720935B1 (en) * 2017-01-10 2017-03-29 호서대학교 산학협력단 Composition for increasing expression level of genes related with growth hormone containing Gupan extracts as effective component
KR20220097747A (en) * 2020-12-31 2022-07-08 강원대학교산학협력단 Composition containing small molecular weight enzyme hydrolysates of black goat extracts and effective peptide sequence

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100475833B1 (en) * 1998-01-24 2005-12-26 학교법인 호서학원 High yield peptide preparation method
KR20010025612A (en) * 2001-01-11 2001-04-06 조석형 Method for preparing a peptide and low molecular weight peptides
KR100566938B1 (en) * 2005-05-14 2006-04-03 한국식품연구원 Process for preparing crude peptide from amyda japonica
KR101720935B1 (en) * 2017-01-10 2017-03-29 호서대학교 산학협력단 Composition for increasing expression level of genes related with growth hormone containing Gupan extracts as effective component
KR20220097747A (en) * 2020-12-31 2022-07-08 강원대학교산학협력단 Composition containing small molecular weight enzyme hydrolysates of black goat extracts and effective peptide sequence

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