KR960016703A - 조직 배양 방법 및 장치 - Google Patents
조직 배양 방법 및 장치 Download PDFInfo
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- KR960016703A KR960016703A KR1019940029214A KR19940029214A KR960016703A KR 960016703 A KR960016703 A KR 960016703A KR 1019940029214 A KR1019940029214 A KR 1019940029214A KR 19940029214 A KR19940029214 A KR 19940029214A KR 960016703 A KR960016703 A KR 960016703A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Zoology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
본 발명은 생화학적 및 형태학적 효율성을 높인 조직 배양 방법 및 이를 실시하기에 적합한 조직 배양 장치에 관한 것으로, 배양 조직이 얹힌 메쉬와 배양 배지가 들어 있는 배양관을 일정 속도로 회진시키면서 조직의 배양을 수행하는 방법에 있어서, 배양 배지와 접촉하는 메쉬의 면적과 기체와 접촉하는 메쉬의 면적이 1 : 3의 비율로 유지되도록 하는 것을 특징으로 하는 조직 배양 방법 및 이를 실시하기에 적합하도록 된 장치는, 형태학적 및 생화학적 효율성이 높은 조직을 배양할 수 있으므로 조직의 구조 및 기능에 관한 연구에 유용한 것이 될 수 있다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (7)
- 배양 조직이 얹힌 메쉬와 배양 배지가 들어 있는 배양관을 일정 속도로 회전시키면서 조직의 배양을 수행하는 방법에 있어서, 배양 배지와 접촉하는 메쉬의 면적과 기체와 접촉하는 메쉬의 면적이 1 : 3의 비율로 유지되도록 하는 것을 특징으로 하는 조직 배양 방법.
- 제1항에 있어서, 상기 메쉬가 상기 배지와 접촉하는 시간과 공기와 접촉하는 시간이 1 : 3으로 유지되는 것을 특징으로 하는 방법.
- 제1항 또는 제2항에 있어서, 상기 메쉬가 정사각기둥의 형태로 되는 것을 특징으로 하는 방법.
- 제1항에 있어서, 상기 배양관이 5 내지 8 rpm 정도의 속도로 회전되는 것을 특징으로 하는 방법.
- 밀봉 가능한 원통형 배양관 ; 상기 배양관 내부에 꼭 맞도록 된 정사각기둥 형의 메쉬 ; 상기 배양관을 회전시키는 수단 ; 및 상기 배양관에 멸균된 기체를 고압으로 공급하는 수단을 포함하는 것을 특징으로 하는 조직 배양 장치.
- 제5항에 있어서, 상기 메쉬가 스테인레스 스틸 재질로 되고 망목의 크기가 200 내지 400㎛ 것을 특징으로 하는 장치.
- 제5항에 있어서 상기 배양관에 공급되는 가스가 1 내지 3atm으로 유지되는 95% O2/5% C02인 것을 특징으로 하는 장치.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019940029214A KR0129833B1 (ko) | 1994-11-09 | 1994-11-09 | 조직 배양 방법 및 장치 |
US08/555,600 US5773285A (en) | 1994-11-09 | 1995-11-09 | Static organ culture apparatus |
US08/788,253 US5795710A (en) | 1994-11-09 | 1997-01-27 | Method and apparatus for organ culture |
US09/006,947 US5976870A (en) | 1994-11-09 | 1998-01-14 | Artificial liver composed of a liver-slice culture apparatus |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019940029214A KR0129833B1 (ko) | 1994-11-09 | 1994-11-09 | 조직 배양 방법 및 장치 |
Publications (2)
Publication Number | Publication Date |
---|---|
KR960016703A true KR960016703A (ko) | 1996-06-17 |
KR0129833B1 KR0129833B1 (ko) | 1998-04-04 |
Family
ID=19397380
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019940029214A KR0129833B1 (ko) | 1994-11-09 | 1994-11-09 | 조직 배양 방법 및 장치 |
Country Status (2)
Country | Link |
---|---|
US (2) | US5773285A (ko) |
KR (1) | KR0129833B1 (ko) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
USRE40570E1 (en) | 1994-07-29 | 2008-11-11 | Edwards Lifesciences Corporation | Apparatuses and methods for treating biological tissue to mitigate calcification |
US5931969A (en) * | 1994-07-29 | 1999-08-03 | Baxter International Inc. | Methods and apparatuses for treating biological tissue to mitigate calcification |
US5976870A (en) * | 1994-11-09 | 1999-11-02 | Park; Sung-Su | Artificial liver composed of a liver-slice culture apparatus |
US6472200B1 (en) | 1999-07-23 | 2002-10-29 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Device and method for performing a biological modification of a fluid |
CA2279996A1 (en) * | 1997-01-16 | 1998-07-16 | Yissum Research Development Company Of The Hebrew University In Jerusale M | A device and method for performing a biological modification of a fluid |
US6214054B1 (en) | 1998-09-21 | 2001-04-10 | Edwards Lifesciences Corporation | Method for fixation of biological tissues having mitigated propensity for post-implantation calcification and thrombosis and bioprosthetic devices prepared thereby |
DE19903876B4 (de) | 1999-02-01 | 2006-09-28 | Orthogen Gentechnologie Gmbh | Verfahren zur in-vitro-Bildung und Anreicherung von Interleukin-1 Rezeptor-Antagonisten |
DE19911326A1 (de) * | 1999-03-15 | 2000-09-28 | Fege Wolfgang | Vorrichtung zum Züchten von menschlichem oder tierischem Gewebe |
US7326564B2 (en) * | 2001-02-20 | 2008-02-05 | St. Jude Medical, Inc. | Flow system for medical device evaluation and production |
US6878168B2 (en) | 2002-01-03 | 2005-04-12 | Edwards Lifesciences Corporation | Treatment of bioprosthetic tissues to mitigate post implantation calcification |
WO2003085080A1 (en) * | 2002-04-04 | 2003-10-16 | Corebiotech Co., Ltd. | Cell culture plate and system using the same |
US6740086B2 (en) | 2002-04-18 | 2004-05-25 | Spinal Innovations, Llc | Screw and rod fixation assembly and device |
US20070048732A1 (en) * | 2005-08-30 | 2007-03-01 | Hepahope, Inc. | Chemosensitivity tester |
US20050130254A1 (en) * | 2003-12-16 | 2005-06-16 | Park Sung-Soo | Drug testing system with bio-artificial liver |
US20040265997A1 (en) * | 2003-06-27 | 2004-12-30 | Park Sung-Soo | Bio-artificial liver system |
GB0410177D0 (en) * | 2004-05-07 | 2004-06-09 | Univ Wales Medicine | Engineered tubular tissue structures |
ITPI20040046A1 (it) * | 2004-06-18 | 2004-09-18 | Univ Pisa | Bioreattore per lo studio degli effetti sulle attivita' cellulari di stimoli imposti |
US7579381B2 (en) * | 2005-03-25 | 2009-08-25 | Edwards Lifesciences Corporation | Treatment of bioprosthetic tissues to mitigate post implantation calcification |
US9493735B2 (en) * | 2005-04-15 | 2016-11-15 | Wake Forest University Health Sciences | Bioreactor system and method of enhancing functionality of muscle cultured in vitro |
CN101626682B (zh) | 2006-10-27 | 2014-04-16 | 爱德华兹生命科学公司 | 用于外科植入的生物组织 |
KR100838104B1 (ko) * | 2007-07-26 | 2008-06-16 | 대한민국 | 세포 발달 촉진 기기 |
US20100261662A1 (en) * | 2009-04-09 | 2010-10-14 | Endologix, Inc. | Utilization of mural thrombus for local drug delivery into vascular tissue |
WO2011119754A2 (en) | 2010-03-23 | 2011-09-29 | Edwards Lifesciences Corporation | Methods of conditioning sheet bioprosthetic tissue |
US8906601B2 (en) | 2010-06-17 | 2014-12-09 | Edwardss Lifesciences Corporation | Methods for stabilizing a bioprosthetic tissue by chemical modification of antigenic carbohydrates |
CA2807762C (en) | 2010-08-13 | 2023-08-08 | Wake Forest University Health Sciences | Methods for making a tissue engineered muscle repair (temr) construct in vitro for implantation in vivo |
US9351829B2 (en) | 2010-11-17 | 2016-05-31 | Edwards Lifesciences Corporation | Double cross-linkage process to enhance post-implantation bioprosthetic tissue durability |
US9358107B2 (en) | 2011-06-30 | 2016-06-07 | Edwards Lifesciences Corporation | Systems, dies, and methods for processing pericardial tissue |
US10238771B2 (en) | 2012-11-08 | 2019-03-26 | Edwards Lifesciences Corporation | Methods for treating bioprosthetic tissue using a nucleophile/electrophile in a catalytic system |
US11466243B2 (en) | 2016-08-03 | 2022-10-11 | University Of Virginia Patent Foundation | Bioreactor controller device and related method thereof |
US10488307B2 (en) * | 2017-12-12 | 2019-11-26 | Dignity Health | Systems, devices, and methods for improved tissue treatment |
EP3778870A4 (en) * | 2018-03-28 | 2022-01-05 | Public University Corporation Yokohama City University | CELLULAR MASS FUSION PROCESS |
GB2587987B (en) * | 2018-05-09 | 2022-12-21 | Emulate Inc | Host-biome interactions |
WO2020076945A1 (en) | 2018-10-11 | 2020-04-16 | Awesome Medical Company, Llc | Plasma dialysis systems and methods of use thereof |
CN110358683B (zh) * | 2019-07-25 | 2022-04-26 | 中国科学院电工研究所 | 一种生物反应器自动控制装置 |
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US2996429A (en) * | 1959-02-12 | 1961-08-15 | Nat Toxicological Lab Inc | Method and apparatus for growing living tissue |
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US3843454A (en) * | 1972-07-31 | 1974-10-22 | Abbott Lab | Flow through tissue culture propagator |
US4442206A (en) * | 1980-08-21 | 1984-04-10 | Stanford University | Method of using isotropic, porous-wall polymeric membrane, hollow-fibers for culture of microbes |
US4537860A (en) * | 1982-12-08 | 1985-08-27 | Monsanto Company | Static cell culture maintenance system |
US4938931A (en) * | 1987-05-26 | 1990-07-03 | Hoechst Celanese Corporation | Microporous membrane trickle bed reactor |
US5585266A (en) * | 1995-10-05 | 1996-12-17 | Plitt; Cheryl A. | Immobilized cell bioreactor |
-
1994
- 1994-11-09 KR KR1019940029214A patent/KR0129833B1/ko not_active IP Right Cessation
-
1995
- 1995-11-09 US US08/555,600 patent/US5773285A/en not_active Expired - Lifetime
-
1997
- 1997-01-27 US US08/788,253 patent/US5795710A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR0129833B1 (ko) | 1998-04-04 |
US5795710A (en) | 1998-08-18 |
US5773285A (en) | 1998-06-30 |
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