KR960013182A - Rapid Mass Production Method of Bottleless Artificial Seed Garlic Using Tissue Culture Technology - Google Patents

Rapid Mass Production Method of Bottleless Artificial Seed Garlic Using Tissue Culture Technology Download PDF

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KR960013182A
KR960013182A KR1019940025775A KR19940025775A KR960013182A KR 960013182 A KR960013182 A KR 960013182A KR 1019940025775 A KR1019940025775 A KR 1019940025775A KR 19940025775 A KR19940025775 A KR 19940025775A KR 960013182 A KR960013182 A KR 960013182A
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shoots
garlic
mass
tissue culture
artificial
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KR1019940025775A
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KR0134140B1 (en
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김주학
정경호
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김희용
동양물산기업 주식회사
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Priority to JP7273656A priority patent/JPH08205703A/en
Priority to CNB951160605A priority patent/CN1166283C/en
Priority to ES09502329A priority patent/ES2109886B1/en
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/35—Bulbs; Alliums, e.g. onions or leeks
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0025—Culture media for plant cell or plant tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/04—Plant cells or tissues

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract

본 발명은 마늘의 생산 수량 감소와 품질의 저하를 초래하는 바이러스 감염문제를 근본적으로 해결하기 위한 방법에 관한 것으로서, 직물 조직 배양 기술을 이용하여 바이러스에 감염되지 않은 무병주 인공 씨마늘(microbulb)을 대량으로 생산하는 방법에 관한 것이다. 본 발명은 무병주 인공 씨마늘의 속규모 생산만 가능한 기존의 무병주 인공 씨마늘 생산 방법과는 다른 획기적으로 개선된 것으로, 본 방법에 따라 저가의 무병주 씨마늘의 대량생산이 가능하다. 본 발명이 방법은 우선 마늘 인편의 정단에서 칼루스를 유도하여 증식시키고, 이로부터 유기한 신초를 재분화시키고, 재분화된 신초로부터 다신초 증식 배지상애서 계대배양에 의해 신초를 대량증식시키고, 대량증식된 신초로부터 인공 씨마늘을 형성시키는 단계로 구성되어 있다. 종래의 방법들에 비하여, 신초의 생산율을 혁신적으로 증대시켰기 때문에 상대적으로 월등히 적은 노동력, 시간, 및 경지로 씨마늘을 대량생산할 수 있게 된다. 또한 현재까지는 높은 생산 원가 때문에 우량 품종의 선발 및 산업적 대량증식이 불가능 했었는데, 본 발명의 다신초를 이용한 반영구적인 대량증식 방법을 통해 지금까지는 실용화 측면에서 불가능했던 우량 형질을 갖는 품종의 대량 증식도 가능하게 되어, 새로운 품종의 선발과 육성이 가능하게 되었다.The present invention relates to a method for fundamentally solving a virus infection problem that causes a decrease in the production yield and quality of garlic, using a tissue tissue culture technology to prevent disease-free artificial microbulb (virus-free) It relates to a method of mass production. The present invention is a significant improvement from the existing method of producing bottleless artificial seed garlic, which can only produce the genus of bottleless artificial seed garlic, and according to the present method, it is possible to mass-produce low-cost bottleless seed garlic. The method of the present invention first induces and propagates callus at the apical of garlic scales, and regenerates the shoots from them, and then mass-produces shoots by subculture on polycytheria proliferation medium from the replanted shoots. It consists of forming artificial seed garlic from shoots. Compared to the conventional methods, the production rate of shoots has been innovatively increased, which enables mass production of seed garlic with relatively less labor, time, and land. In addition, up to now, the selection of high quality varieties and industrial mass growth were not possible due to the high production cost. Through the semi-permanent mass growth method using polycytheria of the present invention, it is also possible to mass breed varieties having good traits that have not been available until now. As a result, the selection and upbringing of new breeds is possible.

Description

조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산 방법Rapid Mass Production Method of Bottleless Artificial Seed Garlic Using Tissue Culture Technology

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 본 발명의 무병주 인공 씨마늘을 생산하는 방법의 공정도,1 is a process chart of a method for producing a bottleless artificial seed garlic of the present invention,

제6도는 패트리디쉬(petridish)내의 칼루스(callus)증식 배지 위에서 증식되고 있는 칼루스(callus)의 모습을 나타낸 사진,FIG. 6 is a photograph showing the appearance of callus multiplying on callus growth medium in petridish,

제7도는 패트리디쉬(petridish)내의 재분화 배지 위에서 재분화된 신초(shoots)의 모습을 나타낸 사진,FIG. 7 is a photograph showing the appearance of re-differentiated shoots on re-differentiation media in petridish,

제8도는 패트리디쉬(petridish)내의 다신초(multishoots) 증식배지 위에서 대량으로 증식되고 있는 다신초(multishoots)의 모습을 나타낸 사진.FIG. 8 is a photograph showing the appearance of multishoots growing in large quantities on a multishoots growth medium in petridish.

Claims (7)

마늘 인편의 정단에서 칼루스를 유도하여 증식시키고, 이로부터 유기한 신초를 재분화시키고, 재분화된 신초로부터 다신초 증식 배지상에서 계대배양에 의해 신초를 대량증식시키고, 대량증식된 신초로부터 인공 씨마늘을 형성시키는 단계로 구성되는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산 방법.Callus is induced on the tops of garlic scales to proliferate, re-differentiate the organic shoots from them, mass propagation of the shoots by subculture on polycythenic growth medium from the re-differentiated shoots, and artificial seed garlic from mass-proliferated shoots Rapid mass production method of bottle-free artificial seed garlic using tissue culture technology consisting of a step of making. 제1항에 있어서, 재분화된 신초를 다신초 증식 배지에서 계대배양함으로써 신초를 대량증식시킴을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 금속 대량 생산 방법.The method of mass-producing metal of bottle-free artificial seed garlic using a tissue culture technique according to claim 1, characterized in that the shoots are mass-proliferated by subcultured re-differentiated shoots in polycythemia growth medium. 제2항에 있어서, 표준크기의 패트리디쉬 속에서 자란 약 300개의 신초들을 작은 신초 덩어리(약 5개의 신초)로 분할하여 다신초 증식 배지가 담겨져 있는 패트리디쉬에 각각 3-4개의 작은 신초 덩어리들을 옮겨 반영구적으로 계대배양함을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산방법.The method according to claim 2, wherein about 300 shoots grown in a standard sized petri dish are divided into small shoots (about 5 shoots) and each 3-4 small shoots are placed in a petri dish containing polycythenic growth medium. Rapid mass production method of artificial seedless garlic using tissue culture technology characterized by subcultured transfer. 제1항, 2항, 또는 3항 중 어느 한 항에 있어서, 다신초 증식 배지는 엠에스 기본 배지중 암모니아태 질소의 양을 절반으로 줄이고, 슈크로스의 양을 2-3%, 식물 생장조절 물질인 비에이(BA : Benzylaminopurine)를 0.5-2.0㎎/L, 엔에이에에(NAA : 1-Naphthylacetic acid)를 0.05-0.2㎎/L, 한천 0.7%를 첨가하고, 멸균하기 전에 pH 5.8로 맞춘 것임을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산방법.The method of any one of claims 1, 2, or 3, wherein the polycythemia growth medium reduces the amount of ammonia nitrogen in the MS basal medium by half, reduces the amount of sucrose by 2-3%, and plant growth regulator. 0.5-2.0 mg / L of BA (Benzylaminopurine), 0.05-0.2 mg / L of NAA (1-Aphthylacetic acid) and 0.7% of agar were added to pH 5.8 before sterilization. Rapid mass production method of disease-free artificial seed garlic using tissue culture technology, characterized in that. 제1항에 있어서, 대량증식된 신초를 온도 4℃, 광도 100-500lux에서 2개월간 저온처리를 한 후, 구형성 배지에 옮겨 배양함으로써 씨마늘을 형성시킴을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산 방법.The disease-free using tissue culture technology according to claim 1, wherein the mass-proliferated shoots are cold treated for 2 months at a temperature of 4 ° C. and a brightness of 100-500 lux, and then transferred to a spherical medium to form seed garlic. Primary rapid seed production method of artificial garlic. 제5항에 있어서, 구형성 배지는 엠에스 기본 배지에 슈크로서 9%, 엔이에에(NAA : 1-Naphthylacetic acid) 0.1-2.0㎎/L로 조성된 것임을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산 방법.The disease-free strain using tissue culture technology according to claim 5, wherein the spheroidal medium is 9% as MS, and 0.1-2.0 mg / L of NEA (NAA: 1-Naphthylacetic acid) as a basal medium. Method of rapid mass production of artificial seed garlic. 제7항에 있어서, 대량증식된 신초를 구형성 배지가 든 패트리디쉬속에서 4-6℃의 저온과, 낮의 길이 16시간, 밤의 길이 8시간, 광도 500-1000lux의 조간하에서 장기저장함을 특징으로 하는 조직 배양 기술을 이용한 무병주 인공 씨마늘의 급속 대량 생산 방법.8. The method of claim 7, wherein the mass-produced shoots are stored for a long time at low temperatures of 4-6 ° C., 16 hours of day length, 8 hours of night length, and 500-1000 lux intensity in a Petri dish containing spherical medium. A rapid mass production method of disease-free artificial seed garlic using a tissue culture technique. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940025775A 1994-10-08 1994-10-08 Garlic tissue cultivation KR0134140B1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1019940025775A KR0134140B1 (en) 1994-10-08 1994-10-08 Garlic tissue cultivation
JP7273656A JPH08205703A (en) 1994-10-08 1995-09-28 Method of rapid and mass production of non-desease artificial kind garlic by tissue culture technology
CNB951160605A CN1166283C (en) 1994-10-08 1995-10-06 Process for mass-producing virus-free seed bulbs of garlic through plant tissue culture technique
ES09502329A ES2109886B1 (en) 1994-10-08 1995-11-27 PROCESS FOR THE MASS PRODUCTION OF GARLIC SEED BULBS, FREE OF VIRUSES, THROUGH THE VEGETABLE TISSUE CULTIVATION TECHNIQUE.

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KR1019940025775A KR0134140B1 (en) 1994-10-08 1994-10-08 Garlic tissue cultivation

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KR960013182A true KR960013182A (en) 1996-05-22
KR0134140B1 KR0134140B1 (en) 1998-04-18

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ES (1) ES2109886B1 (en)

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KR100259230B1 (en) * 1997-09-01 2000-06-15 장기원 Tissue culture of sundew
KR100375262B1 (en) * 1999-10-13 2003-03-10 (주)유케이케미팜 A Method for Manufacturing Plaunotol by Liquid-Suspension Cell Culture

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KR0134140B1 (en) * 1994-10-08 1998-04-18 김희용 Garlic tissue cultivation
CN1090901C (en) * 1999-06-15 2002-09-18 南京农业大学 Method for quickly multiplying detoxicated garlic seedlings as seed
KR100745591B1 (en) * 2006-05-12 2007-08-02 전라남도 New clonal variety choyoung with incomplete bolting and tropical growth habit being apt for big-bulblets cultivation in allium species
KR101149171B1 (en) * 2009-12-01 2012-05-25 고려대학교 산학협력단 Method for production of virus-free plants and seeds from virus infected plants
CN102499095A (en) * 2012-01-05 2012-06-20 黑龙江省科学院大庆分院 Method for direct induction of bulb culture medium by isolated culture of garlic (Allium sativum L.) rachis
KR101447116B1 (en) * 2012-05-16 2014-10-06 한국생명공학연구원 Method for mass production of artificial seed potato by low temperature incubation and the artificial seed potato produced by the same
CN103598090B (en) * 2013-10-31 2016-08-17 青岛文创科技有限公司 A kind of method utilizing garlic clove induction garlic callus
CN104521754B (en) * 2014-12-10 2016-06-15 湖北省农业科学院经济作物研究所 High mountain ovum leaf fragrant-flowered garlic method for quickly breeding
CN106386500B (en) * 2016-11-03 2019-03-19 广西玉林容山堂生物科技有限公司 A kind of culture medium of garlic tissue cultural seedlings of free
CN109452330B (en) * 2017-11-21 2020-10-20 浙江省嘉兴市农业科学研究院（所） Bacteriostatic agent for plant tissue culture and application of bacteriostatic agent in anoectochilus formosanus tissue culture

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JPS62289193A (en) * 1986-06-09 1987-12-16 Ajinomoto Co Inc Production of sulfur-containing compound such as alliin by tissue culture of garlic
RO96914A2 (en) * 1986-11-10 1989-05-30 Institutul De Cercetari Pentru Legumicultura Si Floricultura,Ro RAPID MOVEMENT PROCESS PROCESS
JPH02200131A (en) * 1989-01-31 1990-08-08 Nippon Steel Corp Multiplication of large amount of plant of genus allium
JPH03160935A (en) * 1989-11-21 1991-07-10 Ajinomoto Co Inc Preparation of plant body
KR0134140B1 (en) * 1994-10-08 1998-04-18 김희용 Garlic tissue cultivation

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100259230B1 (en) * 1997-09-01 2000-06-15 장기원 Tissue culture of sundew
KR100375262B1 (en) * 1999-10-13 2003-03-10 (주)유케이케미팜 A Method for Manufacturing Plaunotol by Liquid-Suspension Cell Culture

Also Published As

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CN1122647A (en) 1996-05-22
ES2109886A1 (en) 1998-01-16
JPH08205703A (en) 1996-08-13
CN1166283C (en) 2004-09-15
ES2109886B1 (en) 1998-10-01
KR0134140B1 (en) 1998-04-18

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