KR100259230B1 - Tissue culture of sundew - Google Patents

Abstract

PURPOSE: A method for culturing tissues of sundew is provided, to massively multiply the tissues for keeping the balance of an ecosystem by subculture. CONSTITUTION: The leaves of sundew are picked and disinfected in a hypochlorous solution 100ml diluted with Tween 2-3 drops. The disinfected leaves are rinsed with distilled water. The rinsed leaves are placed in a 1/2 MS culture medium of pH 5.7, and shoots are abandoned. The subculture is repeated every 3-4 months. The grown young leaves proliferate to several thousands of leaves in a year.

Description

Tissue Culture of Sticky Spatula

The present invention relates to a tissue culture method of a sticky spatula which is a rare plant.

The destruction of biodiversity in the process of industrialization is an unavoidable reality. In Korea, wild plant habitats are rapidly being destroyed during industrialization and urbanization. Preservation of genetic resources and the maintenance of biodiversity have emerged as a global challenge, and the preservation of state systems is a practically important task.

Sticky spatula grows in high atmospheric and soil humidity such as around swamps and wetlands, and Bogildo is a rare plant and is known as the habitat of sticky spatula, a carnivorous plant. This plant is the most representative of all known carnivorous plants and is a perennial herbaceous plant. The name of this plant species comes from the shape of a leaf that resembles the shape of a rice paddle.

The plant secretes a sticky solution at each end of its spine to capture insects and dissolve them with various enzymes to get the nutrients needed for growth. Insects that are captured are fly, fly, etc., and thus are known as self-nourishing foods and taga foods because they are supplied with the necessary nutrients from animals while performing photosynthesis.

These precious resource plants are becoming increasingly extinct due to the recent increase in tourists and the winds of urbanization, which necessitates the conservation of these plant resources. Sticky spatulas have not been studied for tissue culture or mass proliferation.

Therefore, in order to preserve the endangered rare carnivorous plant resources and to balance the ecosystem, the present invention aims to provide mass growth technology by introducing a tissue culture method. It is another object of the present invention to provide optimum culture conditions for tissue culture of a sticky spatula.

Hereinafter, the specific configuration and operation of the present invention.

1 is a photograph of a sticky spatula that is being multiplied by tissue culture.

2 is a sticky spatula redistributed by tissue culture

The seeds of the sticky spatula were collected, sterilized, rinsed with sterile water, seeded in the medium, germinated, and then cut by leaves after a certain period of time, and then etched on a tissue culture medium.

Hereinafter, the specific structure and operation of the present invention will be described with reference to Examples.

Example 1

Growth of seedlings

Sticky spatula seeds were disinfected in 100 ml of finite lacquer and diluted finite lacquer with 2-3 drops of Tween. The sterilized seeds were rinsed with sterile water and then seeded in a Erlenmeyer flask containing MS medium adjusted to pH5.7.

MS medium used is shown in Table 1 below.

TABLE 1

MS medium composition table (mg / l)

Seeds germinated 3-4 weeks later in MS medium.

Germinated seedlings (Seedling) 3-4 months later, some seedlings were dispensed and transferred back to ½MS medium, and some seedlings were cut and transplanted into ½MS medium. This allowed them to transfer to new ½ MS medium every three to four months to complete mass growth and produce thousands of seedlings per year.

1 is a photograph showing the growth of seedlings grown on ½ MS medium from seeds.

Example 2

Tissue culture

Results of Example 1 The leaves harvested from the dispensed aeration were disinfected in an aqueous finite lacx solution used in Example 1, rinsed with sterile water, and then healed in ½ MS medium adjusted to pH5.7. The injured leaves were well sprouted and subcultured again in ½ MS medium (pH5.7) every 3-4 months. The seedlings thus grown were capable of thousands of mass growths a year.

FIG. 1 is a photograph showing a re-divided sticky spatula by transplanting seedlings seeded on ½ MS medium from a cut leaf into a pot.

Experimental Example 1

On the other hand, the effect of MS medium concentration and initial pH on the shoot spatula and tissue culture of sticky spatula was ½ MS medium of pH5.7.

TABLE 2

Suitable time for subcultures

As described above, the present invention is a very useful invention in the bioindustry because it is possible to maintain the diversity of the species by providing the culture medium and tissue culture necessary for the tissue culture of the sticky spatula, which is a rare carnivorous plant useful for protecting the ecosystem.

Claims (1)

The leaves of the sticky spatula were cut out and disinfected in diluted Yuhan-Lax aqueous solution with 2 ~ 3 drops of Tween 20 in 100 ml of Yuhan-Lax, rinsed with sterile water and adjusted to pH 5.7.

A tissue culture method of a sticky spatula, characterized by subcultured every 3 to 4 months, healed and sheathed in MS medium.

Images