KR960010011A - Substances and methods of inhibiting cancer growth from shark cartilage - Google Patents

Substances and methods of inhibiting cancer growth from shark cartilage Download PDF

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Publication number
KR960010011A
KR960010011A KR1019940023477A KR19940023477A KR960010011A KR 960010011 A KR960010011 A KR 960010011A KR 1019940023477 A KR1019940023477 A KR 1019940023477A KR 19940023477 A KR19940023477 A KR 19940023477A KR 960010011 A KR960010011 A KR 960010011A
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South Korea
Prior art keywords
shark cartilage
cancer growth
substance
crude extract
fractional
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KR1019940023477A
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Korean (ko)
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KR0143860B1 (en
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김민영
김성규
강성종
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김정재
주식회사 한일합섬
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Priority to KR1019940023477A priority Critical patent/KR0143860B1/en
Publication of KR960010011A publication Critical patent/KR960010011A/en
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Publication of KR0143860B1 publication Critical patent/KR0143860B1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/60—Fish, e.g. seahorses; Fish eggs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

본 발명은 건조 상어연골 분말로부터 가용성 물질을 추출하고, 추출용매를 제거하여 조 추출액을 얻어, 혈관형성억제작용을 계란의 융모요막에세이로 확인하고, 이 조 추출물을 음이온 교환칼럼을 이용하여 세가지 분획으로 분리한 후 혈관 내피세포의 증식을 억제함을 확인한 후 조 추출액과 한가지의 분획이 고형암의 성장을 억제하는 것을 증명하여, 상어연골로부터 혈관형성억제작용 및 면역증강 작용이 있는 물질의 제조방법이다.The present invention extracts the soluble substance from the dry shark cartilage powder, and removes the extraction solvent to obtain a crude extract, to confirm the inhibition of angiogenesis by chorionic urease assay of eggs, this crude extract using three anion exchange column After the separation, the crude extract and one fraction proved to inhibit the growth of solid cancer, and confirmed that it inhibits the proliferation of vascular endothelial cells. .

Description

상어연골로부터 암의 성장을 억제하는 물질 및 분리 방법Substances and methods of inhibiting cancer growth from shark cartilage

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

건조 상어연골 분말을 구아니딘 클로라이드, 요소 또는 이들의 혼합물이 용해된 MES나 Tris 완충용액을 추출용매로 하여, 진탕 추출하고 추출용매를 제거하여 조 추출물을 얻는 상아연골에서 암성장 억제 물질의 제조방법Method for producing a cancer growth inhibitory substance in ivory cartilage obtained by extracting a crude extract by shaking extraction and removing the extraction solvent using MES or Tris buffer solution containing guanidine chloride, urea or mixture thereof as dry shark cartilage powder 제1항에 있어서, 추출용매를 제거하기 위하여 젤 여과 칼럼 또는 투석막으로 투석함을 특징으로 하는 상어연골에서 암성장 억제 물질의 제조방법[Claim 2] The method for preparing a cancer growth inhibiting substance in shark cartilage according to claim 1, characterized in that it is dialyzed with a gel filtration column or a dialysis membrane to remove the extraction solvent. 제1항에 있어서, 조 추출물을 음이온 교환수지를 이용하여 NaCl염을 함유한 완충용액으로 세종류의 분획(Q1, Q2 및 Q3)으로 용출시키고, 용출후 NaCl염을 제거함을 특징으로 하는 상어연골에서 암성장 억제 물질의 제조방법The shark cartilage according to claim 1, wherein the crude extract is eluted with three kinds of fractions (Q1, Q2 and Q3) using a buffer solution containing NaCl salt using an anion exchange resin, and the NaCl salt is removed after elution. Of cancer growth inhibitory substance in 제3항에 있어서, 음이온 교환수지로는 FPLC의 모노-Q 칼럼이나 디이에이-세파로즈 칼럼을 이용함을 특징으로 하는 상어연골에서 암성장 억제 물질의 제조방법4. The method of claim 3, wherein the anion exchange resin is a mono-Q column or DY-sepharose column of FPLC. 제1항의 방법에 따라 얻어진 암성장 억제 조 추출물Cancer growth inhibitory crude extract obtained according to the method of claim 1 제3항의 방법에 따라 얻어진 암성장을 억제하는 분획물질(Q1, Q2 및 Q3)Fractional substances (Q1, Q2 and Q3) which inhibit cancer growth obtained according to the method of claim 3 제6항에 있어서, 분획물질은 혈관형성을 억제하는 단백질, 콜라겐과 뮤코폴리사카라이드류의 글라이코아미노글리칸으로 구성되어 있음을 특징으로 하는 암성장을 억제하는 분획물질The fractional substance according to claim 6, wherein the fractional substance is composed of a protein that inhibits angiogenesis, and glycoaminoglycans of collagen and mucopolysaccharides. 제7항에 있어서, 뮤코폴리사카라이드는 황산 콘드로이틴 또는 히알우론산임을 특징을로 하는 전이를 억제하는 분획물질8. The fractional substance according to claim 7, wherein the mucopolysaccharide is chondroitin sulfate or hyaluronic acid. 상어연골 추출물의 항암제, 전이억제제 및 과다한 혈관 형성으로 인한 질병에 치료에 사용하는 용도Use of shark cartilage extract in the treatment of cancer, metastasis inhibitors and diseases caused by excessive blood vessel formation 제9항에 있어서, 과다한 혈관 형성으로 인한 질병은 류마치스성 관절염, 골성 관절염, 당뇨병성 망막증, 건선, 혈관증, 세정맥의 변형, 동맥경화성 반점, 각막이식에 의한 혈관형성, 과립성 화상, 혈유병성 관절, 카포시 육종, 녹내장, 황반의 퇴행변성, 장염 또는 망막염증 임을 특징으로 하는 상어연골 추출물의 용도10. The disease according to claim 9, wherein the disease caused by excessive angiogenesis is rheumatoid arthritis, osteoarthritis, diabetic retinopathy, psoriasis, angiopathy, deformity of the varicose veins, atherosclerotic spots, angiogenesis by corneal transplantation, granular burn, hemopathogenic Use of shark cartilage extract characterized by joint, Kaposi's sarcoma, glaucoma, macular degeneration, enteritis or retinitis ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940023477A 1994-09-16 1994-09-16 Anti-cancer agents extracted from cartilage of shark KR0143860B1 (en)

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Application Number Priority Date Filing Date Title
KR1019940023477A KR0143860B1 (en) 1994-09-16 1994-09-16 Anti-cancer agents extracted from cartilage of shark

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Application Number Priority Date Filing Date Title
KR1019940023477A KR0143860B1 (en) 1994-09-16 1994-09-16 Anti-cancer agents extracted from cartilage of shark

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KR960010011A true KR960010011A (en) 1996-04-20
KR0143860B1 KR0143860B1 (en) 1998-07-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010070901A (en) * 2001-06-18 2001-07-27 강헌수 manufacturing method for chondroitin sulfate and its content

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101628982B1 (en) 2015-11-27 2016-06-09 김준혁 Health-supplementary food to improve anticancer activity or immunity function

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010070901A (en) * 2001-06-18 2001-07-27 강헌수 manufacturing method for chondroitin sulfate and its content

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