KR960007382B1 - Organic fertilizer's preparation - Google Patents

Organic fertilizer's preparation Download PDF

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KR960007382B1
KR960007382B1 KR1019930015327A KR930015327A KR960007382B1 KR 960007382 B1 KR960007382 B1 KR 960007382B1 KR 1019930015327 A KR1019930015327 A KR 1019930015327A KR 930015327 A KR930015327 A KR 930015327A KR 960007382 B1 KR960007382 B1 KR 960007382B1
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amino acid
effective
hours
protein
organic fertilizer
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KR950005779A (en
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남용술
남문현
남석현
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남용술
남문현
남석현
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/08Organic fertilisers containing added bacterial cultures, mycelia or the like
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B09DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09BDISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
    • B09B3/00Destroying solid waste or transforming solid waste into something useful or harmless
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

(1) mixing 100kg carbon source contg. 20-40% moisture and steamed 200kg colloid type protein-contg. 60-80% moisture; (2) adding microbiology for digesting into the obtd. mixt. and digesting at room temp. for 36hrs.; and (3) collecting amino acid percolate from the digested mixt. to obtain the amino acid and the fertilizer from the residue.

Description

동물성 단백질의 아미노산 분해추출 및 유기질 비료의 제조방법Amino Acid Degradation Extraction of Animal Protein and Manufacturing Method of Organic Fertilizer

본 발명은 다량의 탄소원을 함유한 물질 즉, 톱밥, 수피, 왕겨, 볏짚 등에 다량의 질소원을 함유한 동물성 단백질을 다량 혼합하여 유효미생물을 첨가 발효시키므로서 탄소원의 분해속도와 단백태 질소원의 분해속도 차이를 이용 과잉의 단백태 질소분해물인 아미노산 용액을 분해추출하고 나머지 잔유물은 완숙된 발효 유기질 비료를 제조하는 방법에 관한 것이다.The present invention is a decomposition rate of carbon source and protein nitrogen source by fermenting the effective microorganisms by adding a large amount of animal protein containing a large amount of nitrogen source, such as sawdust, bark, rice husk, rice straw, etc. The difference is used to decompose and extract the excess protein nitrogen decompose amino acid solution and the remaining residue is to produce a mature fermented organic fertilizer.

종래의 단백질을 분해하는 방법으로 화학적 방법으로는 단백질 물질(육류, 콩)에 염산 또는 황산 등 강산을 단백질 물질에 투입한 후 강열하에 48시간 이상 분해하므로서 산염류의 집적과 공정상의 위험 및 유해성이 뒤따르고 유효아미노산의 파괴(멸실) 등의 문제점이 있었다.In the conventional method of decomposing proteins, chemical methods include adding hydrochloric acid or sulfuric acid to protein materials (meat, soybean) to protein materials, and then decomposing them for more than 48 hours under intense heat. This was followed by problems such as destruction of the effective amino acid.

또 종래의 미생물로 분해하는 방법은 단백질 물질만을 수용성 및 고형의 배지(영양원)로 짧게는 15일, 길게는 5개월의 장기간에 걸친 분해로 분해 조건이 다른 각각의 유효아미노산의 멸실율이 높고 일부는 아미노기까지의 분해진행이 이루어지지 않는 단점과 막대한 경비가 소요되어 제조원가가 매우 높은 결점이 있었다.In addition, the conventional method of decomposing into a microorganism is a water-soluble and solid medium (nutrient source) only for 15 days and a long period of 5 months, so that the effective rate of destruction of each effective amino acid with different degradation conditions is high. The disadvantage of not being able to proceed to the amino group decomposition and enormous cost was a defect in the manufacturing cost is very high.

본 발명은 목재폐기물이나 농산폐기물인 탄소원 즉, 톱밥, 수피, 왕겨, 볏집 등을 이용하므로서 제품의 원가를 현저히 줄일 수 있으며 또한 과잉의 아미노산 용액만을 미생물에 의하여 분리(침출)추출하므로서 각종의 유효아미노산을 골고루 함유한 제품을 생산할 수 있으며 또한 탄소함유 유기물의 최종 잔유물을 유효 미생물에 의하여 최적의 부숙도와 C/N율을 적합하게 유지된 고급의 발효유기질 비료를 얻을 수 있는 방법으로 이를 구체적으로 설명하면 수분 20%-40%를 함유한 100㎏의 탄소원에 수분 60%-80%의 수증기로 증자한 교질상 동물성 단백질 200㎏을 균일하게 혼합하고 여기에 미량의 유효분해미생물을 첨가한 다음 침출용액의 포집이 가능한 경사진 개폐용기에 넣고 완전 소화하여 아미노산 침출용액을 얻고 나머지 잔유물에 상기와 동일량의 동물성 단백질과 유효분해미생물을 첨가하는 공정을 2-3회 되풀이 하여 아미노산 분해추출용액을 얻고 나머지 잔유물인 유기질 비료를 얻는 방법이라 할 수 있다.The present invention can significantly reduce the cost of the product by using wood waste or agricultural waste carbon source, that is, sawdust, bark, rice hulls, crests, etc. Also, by separating (extracting) the excess amino acid solution by microorganisms, various effective amino acids In order to produce a product containing evenly, and the final residue of the carbon-containing organic matter by the effective microorganisms in order to obtain a high-quality fermented organic fertilizer with the optimum maturity and C / N ratio properly maintained 100 kg of carbon source containing 20% -40% water and 200kg of gelatinous animal protein with 60% -80% water vapor were evenly mixed, and a small amount of effective degrading microorganism was added thereto. Put it in the inclined openable container which can be collected and digest it completely to obtain amino acid leaching solution. Repeatedly two or three times a step of adding a protein and an effective decomposing microbial decomposition to obtain the amino acid extracting solution can be called a method of obtaining the remaining residues of organic fertilizer.

여기에서 유효분해미생물이라함은 바실러스크로스트리비움, 플라보박테리움, 스모악티노마이세스, 스트렙토마이세스, 아스펠리길루스올리제 등의 통상적인 미생물이라 할 수 있다.Herein, effective microorganisms may be referred to as conventional microorganisms, such as Bacillus crostridium, Flavobacterium, Smoactinomyces, Streptomyces, Aspergillus olise.

상기 방법에서는 C/N율 즉, 과잉의 동물성 단백태를 사용하는 것이 특징이라 할 수 있고 식물성 섬유인 탄소원과 동물성 단백태와는 소화되는 속도의 차이가 있으므로 탄소원을 함유한 톱밥, 왕겨, 수피, 볏짚 등이 본 방법에서 사용된 유효분해미생물에 의해 소화되는 시간은 동물성 단백태가 소화되는 시간의 약 3배가 되므로 1차 아미노산 침출용액을 얻고 남은 잔유물에 2회 내지 3회까지 동물성 단백태와 미량의 유효분해미생물을 첨가하여 아미노산 침출용액을 얻게 된다. 이때 추가되는 단백태는 1차 사용과 같거나 그 이하가 되며 유효분해미생물은 소화 상태에 따라 적절히 첨가한다. 이때 3회의 아미노산 용액을 얻고 남은 잔유물은 탄소원이 완전히 소화된 상태이고 최적의 부숙도와 C/N율이 적합하게 유지된 고급의 발화 유기질 비료라 할 수 있다.In the above method, C / N ratio, that is, it may be characterized by using excess animal protein, and there is a difference in digestion rate between the carbon source and the animal protein, which is vegetable fiber, so sawdust, rice hull, bark, The time for rice straw to be digested by the effective microorganisms used in this method is about three times the time for digesting the animal protein, so the first amino acid leaching solution is obtained and the remaining protein residues are contained in animal protein and traces up to two to three times. An effective leaching microorganism of is added to obtain an amino acid leaching solution. At this time, the added protein is the same as or less than the first use, and the effective microorganism is added according to the digestion state. At this time, the residue obtained after three times the amino acid solution is a high-ignition organic fertilizer in which the carbon source is completely digested and the optimum maturity and C / N ratio is properly maintained.

그리고 소화공정은 약 25℃의 상온에서 시작하여 점차시간이 갈수록 온도가 상승하며 약 하루반이 지나면 온도가 80℃로 상승하고 이 상태로 약 24시간이 유지된 후 온도가 하강하기 시작한다. 아미노산의 침출은 약 70℃에서부터 조금씩 과잉의 동물성 아미노산 용액이 하단부로 침출하기 시작하여 약 80℃ 정도에서 가장 많은 양이 결집된다. 또 1차 아미노산 용액을 얻고난 잔유물은 소화가 진행중인 톱밥, 왕겨, 수피 등으로서 이들은 아미노산 용액을 통과시키는 기능을 갖고 있지만 완전 소화되면 아미노산 용액을 통과시킬 수 있는 기능이 없어지고 아미노산 용액과 혼합되어 침출되므로 아미노산 용액의 순도가 떨어지고 불순물이 다량 함유되므로 약 3회까지 사용함이 바람직하며 이때 탄소원은 부속도가 가장 좋은 상태이다.And the digestion process starts at room temperature of about 25 ℃ gradually increases the temperature over time, and after about a day and a half, the temperature rises to 80 ℃ and the temperature starts to fall after about 24 hours is maintained in this state. The leaching of amino acids starts from about 70 ° C., and the excess of the animal amino acid solution begins to leap to the lower end, whereby the largest amount is collected at about 80 ° C. Residues obtained from the first amino acid solution are sawdust, rice hulls, bark, etc., which are in the process of digestion. They have the ability to pass the amino acid solution, but when fully digested, they lose the ability to pass the amino acid solution. Therefore, since the purity of the amino acid solution is reduced and contains a large amount of impurities, it is preferable to use about three times.

그밖에 본 방법에서 동물성 단백태로서는 폐어류, 폐육류, 폐가죽, 기타 동물성 유기물을 사용하지만 이를 그대로 소화시키는 것에 비해 고온의 수증기로 증자하여 교질화된 상태로 소화공정에 도입하면 소화율을 약 70%에서 95%까지 상승시킬 수 있으며 유효분해미생물로는 세균, 방선균, 곰팡이균을 사용한다.In addition, animal protein in this method uses waste fish, waste meat, waste leather, and other animal organic matter. However, when digested as it is, it is steamed by hot steam and heated in gelatinized state. It can increase up to 95% and bacteria, actinomycetes and fungi are used as effective degrading microorganisms.

본 방법의 이해를 돕고 효과를 나타내기 위하여 실시예를 들어 설명하면 아래와 같다.In order to help the understanding of the method and to demonstrate the effect of the following examples.

[실시예]EXAMPLE

수분 30%, 톱밥 및 왕겨 100㎏에 수분 70%의 고온증기로 처리한 교질화된 동물성 담백태(폐어육과 폐육류 트일링 혼합물) 미량의 유효미생물(세균, 방선균, 곰팡이균의 혼합물)을 균일하게 혼합하여 아미노산과 침출 용액의 포집이 가능한 경사진 개폐용기에 넣고 상온에서 발효를 시작하여 5시간마다 온도를 측정하였더니 최초 5시간 후 40℃가 되었으며, 다시 5시간 후 43℃가 되었으며, 다시 5시간 후 49℃가 되었으며, 다시 5시간 후 59℃가 되었으며, 다시 5시간 후 69℃가 되었으며, 다시 5시간 후 72℃가 되었으며, 다시 5시간 후 80℃가 되었으며, 그후 24시간 정도 최고의 온도를 유지하다가 서서히 발효온도가 하강하기 시작하였다. 아미노산 용액의 침출은 70℃ 정도부터 조금씩 과잉의 아미노산 용액이 하단부로 침출하기 시작하여 80℃정도에서 가장 많은 양이 포집되었다. 70℃-80℃를 유지하는 18시간 동안 수분 64% 아미노산용액 80ℓ (총포집량 89ℓ중 약 90%에 해당하는 양임)가 포집되었다. 나머지 잔류톱밥 및 왕겨에 2차로 동물성 단백태 100㎏과 미량의 유효분해미생물을 추가하여 재차 소화한 결과 60ℓ의 아미노산 용액을 얻었고 3차에서 50ℓ의 아미노산 용액을 얻었다.Uniform microorganisms (microbial mixture of bacteria, actinomycetes, and fungi) of microorganisms of mixed light and white meat (mixture of waste fish meat and waste meat) treated with 30% moisture, 100 kg of sawdust and rice hulls with 70% moisture The mixture was placed in an inclined opening and closing container capable of capturing amino acids and leaching solutions. The fermentation was started at room temperature, and the temperature was measured every 5 hours. The temperature became 40 ° C after the first 5 hours, and 43 ° C after 5 hours. After 5 hours, it became 49 ℃, again after 5 hours became 59 ℃, again after 5 hours became 69 ℃, again after 5 hours became 72 ℃, again after 5 hours became 80 ℃, and then after 24 hours The fermentation temperature began to drop slowly. The leaching of the amino acid solution began to leach excess amino acid solution to the lower part from about 70 ℃ little by little, and the largest amount was collected at about 80 ℃. Eighty liters of water 64% amino acid solution (corresponding to about 90% of the total collection amount of 89 liters) was collected for 18 hours at 70-80 캜. The remaining digested sawdust and rice hulls were digested again by adding 100 kg of animal protein and a small amount of effective biodegradable microorganisms to obtain a 60 L amino acid solution and a 50 L amino acid solution in the third.

3회 사용 후의 잔유물을 비료공정시험법으로 분석하였더니 C/N율 15% N : 1.8%, P2O5: 0.52, K2O : 0.36%의 완숙된 비료의 분석치를 얻었다.The residue after three times was analyzed by the fertilizer process test. As a result, analysis of the finished fertilizer with a C / N ratio of 15% N: 1.8%, P 2 O 5 : 0.52, and K 2 O: 0.36% was carried out.

한편, 상기 방법으로 얻어진 아미노산 용액중 유효아미노산을 분석하였더니 (표 1)과 같은 결과를 얻었다.On the other hand, an effective amino acid in the amino acid solution obtained by the above method was analyzed, and the results as shown in Table 1 were obtained.

[표 1]TABLE 1

유효아미노산 분석표(㎎/100㎖)Effective amino acid analysis table (mg / 100mL)

이와 같이 많은 종류의 유효아미노산이 다량으로 함유된 것이 분석되었으며 또한 간단한 수경시비재배시험을 5%의 아미노산 용액으로 상추에 시비한 결과 대조구보다 약 15%의 수율증가 얻었고 대조구의 상추보다 잎상이 더욱 부드러우면서도 저장성이 뛰어났다.As a result, a large amount of effective amino acid was analyzed, and a simple hydroponic fertilization test was applied to lettuce with 5% amino acid solution, yielding a yield of about 15% compared to the control and softer leaf than that of the control. Wetting was excellent.

이상과 같이 본 발명은 동물성 유기물, 식물성 탄소원의 산업폐기물로 인한 공해요인을 저감시키고 아미노산 용액과 우수한 유기질 비료를 얻게 되는 산업폐기물이 재활용 차원에서 적극 장료해야 할 사업으로 유기농업의 우수한 비료자체가 되는 고급의 유효아미노산 및 발효유기질 비료의 제조방법이라 할 수 있다.As described above, the present invention is to reduce pollution caused by industrial wastes of animal organic matters and vegetable carbon sources, and to obtain industrial amino acids and organic fertilizers. It can be said to be a method for producing high effective amino acids and fermented organic fertilizers.

Claims (1)

수분 20%-40%를 함유한 100㎏의 탄소원에 수증기를 증자한 수분 60%-80%의 교질상 단백질 200㎏을 균일하게 혼합하고, 여기에 미량의 유효분해미생물을 첨가한 다음 침출용액의 포집이 가능한 경사진 개폐용기에 넣고 상온에서 소화를 시작하여 36시간 소화하여 온도가 80℃로 유지된 상태에서 24시간에 걸려 아미노산 침출용액을 얻고 나머지 잔유물에 상기와 동일량의 동물성 단백질과 유효분해미생물을 첨가하는 공정을 2-3회 되풀이 하여 아미노산 분해추출물을 얻고 나머지 잔유물을 비료로 얻게 됨을 특징으로 하는 동물성 단백질의 아미노산 분해추출 및 유기질 비료의 제조방법.100 kg of carbon source containing 20% -40% water and 200kg of water vapor 60% -80% gelatinous protein were mixed uniformly, and a small amount of effective microorganism was added thereto, followed by Put it in an inclined openable container and start digestion at room temperature, digest it for 36 hours, get an amino acid leaching solution for 24 hours under the temperature maintained at 80 ℃, and effectively dissolve the same amount of animal protein as the above in the remaining residue. A method for producing amino acid decomposition and organic fertilizer of animal protein, characterized in that the process of adding the microorganism is repeated 2-3 times to obtain the amino acid decomposition extract and the remaining residue as a fertilizer.
KR1019930015327A 1993-08-06 1993-08-06 Organic fertilizer's preparation KR960007382B1 (en)

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KR960007382B1 true KR960007382B1 (en) 1996-05-31

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