KR950032638A - 탄소 졸 표지물을 이용한 면역에세이 방법 - Google Patents

탄소 졸 표지물을 이용한 면역에세이 방법 Download PDF

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KR950032638A
KR950032638A KR1019950011679A KR19950011679A KR950032638A KR 950032638 A KR950032638 A KR 950032638A KR 1019950011679 A KR1019950011679 A KR 1019950011679A KR 19950011679 A KR19950011679 A KR 19950011679A KR 950032638 A KR950032638 A KR 950032638A
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반 아메론겐 아트
헤르만 비허스 얀
베르나르디나 요한나 마리아 베렌센 루시엔
빌렘 야콥 반 도른 알버트
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에이. 에이치. 에닝크
딘스트 랜드보쿤디히 온더조크
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Abstract

본 발명은 콜로이드 탄소 입자의 표면에 직접 컨쥬게이트된, 분석물을 특이적으로 인지할 수 있는 결합성분을 가지는 콜로이드 탄소 입자인 다음 식으로 표시되는 선형 예측값이 V＞0인 카본 블랙의 본질적으로 안정화되지 않은 수성 탄소 졸로 이루어진 탄소 표지된 성분과 샘플을 접촉시키고, 상기 샘플에서 분석물의 존재 또는 그 양의 표시로써 생성된 분석물/탄소 입자 복합체의 존재 여부를 측정하는 것으로 이루어진 상기 샘플에서 분석물의 존재 또는 양을 측정하는 방법, 이 방법에 사용하기에 적당하는 조성물 및 그의 제조방법에 관한 것이다.
V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
상기 식에서, DBP는 DIN 53601에 따라 측정된 다부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측징된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.

Description

탄소 졸 표지물을 이용한 면역에세이 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 수성매질내에서 선형 예측값(V)과 Degussa/Cabot 미십자 카본 블랙 분산성 간의 연관성을 나타낸 것으로, 실험데이타(Disp.1 또는 Disp.0)는 계산된 V에 대하여 플로트되었다.

Claims (14)

  1. 다음 식으로 표시되는 선형 예측값(V)이 V＞0인 카본 블랙을 탄소-표지된 성분을 제조하는데 사용되는 본질적으로 안정화되지 않는 수성 탄소졸을 제조하기 위해 사용하는 것을 특징으로 하는, 콜로이드 탄소 입자의 표면에 직접 컨쥬게이트된, 분석물을 특이적으로 인지할 수 있는 결합성분을 가지는 수성 탄소 졸로 이루어진 탄소 표지화된 성분과 샘플을 접촉시키고, 상기 샘플에서 분석물의 존재 또는 그 양의 표시로써 생성된 분석물/탄소 입자 복합체의 존재 여부를 측정하는 것으로 이루어진 샘플에서 분석물의 존재 또는 양을 측정하는 방법.
    V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
    상기 식에서, DBP는 DIN 53601에 따라 측정된 디부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측정된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.
  2. 제1항에 있어서, 분석물이 세포 표면에 존재하는 리셉터 단백질과 항원결정기로부터 선택되는 것인 방법.
  3. 제1항에 있어서, 분석물이 불완전 항원, 항원, 효소, 항체와 항체절편, DNA 및 RNE로 이루어진 군에서 선택되는 것인 방법.
  4. 제1항에 있어서, 콜로이드 탄소 입자에 컨쥬게이트된 결합성분이 불완전 항원, 항원, 효소, 항체와 항체절편, 리셉터 단백질, 항원결정기, DNA 및 RNA로 이루어진 군에서 선택되는 것인 방법.
  5. 제1항에 있어서, 상기 방법이 고체상 면역에세이인 것인 방법.
  6. 제1항에 있어서, 상기 방법이 면역크로마토그래피 에세이인 것인 방법.
  7. 제1항에 있어서, 상기 방법이 딥스틱 면역에세이인 것인 방법.
  8. 제1항에 있어서, 1차 콜로이드 탄소 입자의 평균 입도가 1 내지 100nm 범위인 것인 방법.
  9. 제1항에 있어서, 본질적으로 안정화되지 않은 수성 탄소 졸은 졸-안정화제를 함유하지 않는 것인 방법.
  10. 수성 탄소 졸이 다음 식으로 표시되는 선형 예측값이 V＞0인 카본 블랙으로부터 유도된 본질적으로 안정화되지 않은 수성 탄소 졸인 것을 특징으로 하는, 콜로이드 탄소 입자의 표면에 직접 컨쥬게이트된, 분석물을 특이적으로 인지할 수 있는 결합성분을 가지는 수성 탄소 졸을 포함하는, 샘플 내의 분석물 측정에 유용한 조성물.
    V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
    상기 식에서, DBP는 DIN 53601에 따라 측정된 디부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측정된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.
  11. 제10항에 있어서, 콜로이드 탄소 입자에 컨쥬게이트된 결합성분이 불완전 항원, 항원, 효소, 항체와 항체절편, 리셉터 단백질, 항원결정기, DNA 및 RNA로 이루어진 군에서 선택되는 것인 조성물.
  12. 수성 탄소 졸이 다음 식으로 표시되는 선형 예측값이 V＞0인 카본 블랙으로부터 유도된 본질적으로 안정화되지 않은 수성 탄소 졸인 것을 특징으로 하는, 수성 탄소 졸을 제조하고 분석물울 특이적으로 인지할수 있는 결합성분을 콜로이드 탄소 입자의 표면에 직접 컨쥬게이트하는 것으로 이루어진 샘플 내의 분석물을 측정하는데 유용한 조성물의 제조방법.
    V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
    상기 식에서, DBP는 DIN 53601에 따라 측정된 디부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측정된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.
  13. 콜로이드 탄소 입자의 표면에 직접 컨쥬게이트된, 불완전 항원, 항원, 효소, 항체와 항체절편, 리셉터 단백질, 항원결정기, DNA 및 RNA로 이루어진 군에서 선택된 물질을 가지는 본질적으로 안정화되지 않은 수성 탄소 졸을 제조하기 위한 다음 식으로 표시되는 선형 예측값이 V＞0인 카본 블랙의 용도.
    V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
    상기 식에서, DBP는 DIN 53601에 따라 측정된 디부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측정된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.
  14. 불완전 항원, 항원, 효소, 항체와 항체 절편, 리셉터 단백질, 항원 결정기, DNA 및 RNA로 이루어진 군에서 선택된 물질을 표지하여 탄소-표지된 면역에세이 시약을 제조하기 위한 다음 식으로 표시되는 선형 예측값이 V＞0인 카본 블랙의 본질적으로 안정화되지 않은 수성 탄소 졸의 용도.
    V=-138.954-0.987XDBP+15.609XVC+3.994XPPD
    상기 식에서, DBP는 DIN 53601에 따라 측정된 디부틸프탈레이트 흡착 (㎖/100g)이고; VC는 DIN 53552에 따라 측정된 휘발률(%)이며; PPD는 평균 1차 입자 직경(nm)이다.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950011679A 1994-05-12 1995-05-12 탄소졸표지물을이용한면역에세이방법 KR100356596B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP08/241,734 1994-05-12
US08/241,734 US5529901A (en) 1987-11-19 1994-05-12 Method for determining the presence or amount of analyte using a stable colloidal carbon sol
US08/241734 1994-05-12
EP94201817.7 1994-06-23
EP94201817A EP0682255A1 (en) 1994-05-12 1994-06-23 Immunoassays using a carbon sol label

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US5120643A (en) * 1987-07-13 1992-06-09 Abbott Laboratories Process for immunochromatography with colloidal particles
US5529901A (en) * 1987-11-19 1996-06-25 Staat Der Nederlanden Method for determining the presence or amount of analyte using a stable colloidal carbon sol
US5922551A (en) * 1997-03-20 1999-07-13 Accumetrics, Inc. Agglutrimetric platelet binding assays in blood
US6623787B2 (en) * 2001-07-26 2003-09-23 Electrochemicals Inc. Method to improve the stability of dispersions of carbon
US20080299047A1 (en) * 2003-07-31 2008-12-04 National Cheng Kung University Method for preparation of water-soluble and dispersed iron oxide nanoparticles and application thereof
US7465587B2 (en) 2004-12-03 2008-12-16 Genzyme Corporation Diagnostic assay device
CA2719036A1 (en) 2008-04-02 2009-10-08 Vivacta Ltd A method for sensing a chemical
GB201105828D0 (en) 2011-04-06 2011-05-18 Vivacta Ltd A device for detecting an analyte
EP3935581A4 (en) 2019-03-04 2022-11-30 Iocurrents, Inc. DATA COMPRESSION AND COMMUNICATION USING MACHINE LEARNING

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US4760030A (en) * 1984-09-10 1988-07-26 Syntex (U.S.A.) Inc. Quantitative opaque particle agglutination assay
US5529901A (en) * 1987-11-19 1996-06-25 Staat Der Nederlanden Method for determining the presence or amount of analyte using a stable colloidal carbon sol
NL8702769A (nl) * 1987-11-19 1989-06-16 Holland Biotechnology Werkwijze voor het bepalen in een testmonster van componenten van de reactie tussen een specifiek bindend eiwit en de bijbehorende bindbare stof onder toepassing van ten minste een gemerkte component, werkwijze voor het bereiden van de gemerkte component, en testkit voor de bepaling van immunocomponenten.
US5252496A (en) * 1989-12-18 1993-10-12 Princeton Biomeditech Corporation Carbon black immunochemical label

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US5641689A (en) 1997-06-24
US5529901A (en) 1996-06-25
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EP0682255A1 (en) 1995-11-15
TW438974B (en) 2001-06-07

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