KR950011613A - Method for preparing immobilized enzyme and method for preparing N-carbamoyl-D-amino acid from hydantoin derivative - Google Patents

Method for preparing immobilized enzyme and method for preparing N-carbamoyl-D-amino acid from hydantoin derivative Download PDF

Info

Publication number
KR950011613A
KR950011613A KR1019930022850A KR930022850A KR950011613A KR 950011613 A KR950011613 A KR 950011613A KR 1019930022850 A KR1019930022850 A KR 1019930022850A KR 930022850 A KR930022850 A KR 930022850A KR 950011613 A KR950011613 A KR 950011613A
Authority
KR
South Korea
Prior art keywords
enzyme
carbamoyl
preparing
immobilized
immobilized enzyme
Prior art date
Application number
KR1019930022850A
Other languages
Korean (ko)
Other versions
KR970002668B1 (en
Inventor
김학성
김동만
Original Assignee
천성순
한국과학기술원
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 천성순, 한국과학기술원 filed Critical 천성순
Priority to KR1019930022850A priority Critical patent/KR970002668B1/en
Publication of KR950011613A publication Critical patent/KR950011613A/en
Application granted granted Critical
Publication of KR970002668B1 publication Critical patent/KR970002668B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02002—Dihydropyrimidinase (3.5.2.2), i.e. hydantoinase

Abstract

본 발명은 다공성 마그네슘 알루미늄 실리케이트에 효소를 흡착시키는 방식의 고정화 효소를 제조하는 방법 및 이에 따라 제조된 고정화 효소를 사용하여 N-카르바모일-D-아미노산을 제조하는 방법에 관한 것이다. 본 발명에 따라 제조된 고정화 효소는 매우 높은 조업 안정성을 나타내며, pH와 온도에 대한 특성에 있어서도 유리 효소의 경우보다 더 넓은 범위에서 높은 효소 활성을 나타낸다.The present invention relates to a method for preparing an immobilized enzyme in a manner of adsorbing an enzyme to a porous magnesium aluminum silicate and a method for producing N-carbamoyl-D-amino acid using the immobilized enzyme prepared accordingly. The immobilized enzyme prepared according to the present invention exhibits very high operational stability and also exhibits high enzymatic activity in a broader range than in the case of free enzymes in terms of pH and temperature.

Description

고정화 효소의 제조 방법 및 이를 사용하여 히단토인 유도체로부터 N-카르바모일-D-아미노산을 제조하는 방법Method for preparing immobilized enzyme and method for preparing N-carbamoyl-D-amino acid from hydantoin derivative

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 흡착 방법에 의해 제조된 고정화 효소와 유리 조효소(crude free enzyme)의 시간 경과에 따른 효소 활성치의 변화를 나타내는 그래프.1 is a graph showing changes in enzyme activity over time of immobilized enzyme and crude free enzyme prepared by the adsorption method of the present invention.

Claims (7)

고정화 담체로서 다공성 마그네슘 알루미늄 실리케이트를 사용하여 효소를 고정화시키는 것을 특징으로 하는 고정화 효소의 제조 방법.A method for producing an immobilized enzyme, characterized in that the enzyme is immobilized using porous magnesium aluminum silicate as an immobilized carrier. 제1항에 있어서, 상기 효소가 슈도모나스, 바실러스, 아그로박터 또는 아쓰로박터 균주로부터 균주로부터 추출한 용액을 pH 조절하여 PEG 6000에 용해 처리하여 얻은 것인 방법.The method according to claim 1, wherein the enzyme is obtained by dissolving the solution extracted from strains from Pseudomonas, Bacillus, Agrobacter or Asrobacter strains in PEG 6000 by adjusting the pH. 제1항에 있어서, 상기 효소가 히단토이나제인 방법.The method of claim 1, wherein the enzyme is hydantoinase. 제1항 내지 3항 중 어느 하나의 항에 있어서, 고정화 효소가 글루타르알데히드를 포함한 가교제로 더 처리되는 것인 방법.The method of claim 1, wherein the immobilized enzyme is further treated with a crosslinking agent comprising glutaraldehyde. 제1항 내지 제4항 기재의 방법에 따라 제조된 고정화 효소.Immobilized enzyme prepared according to the method according to claim 1. 제5항 기재의 고정화 효소를 연속 반응기 중에서 히단토인 유도체에 작용시키는 것을 특징으로 하는 N-카르바모일-D-아미노산의 제조 방법.A method for producing N-carbamoyl-D-amino acid, wherein the immobilized enzyme according to claim 5 is reacted with a hydantoin derivative in a continuous reactor. 제6항에 있어서, 고정화 효소는 고정화 히단토이나제이고, N-카르바모일-D-아미노산은 N-카르바모일-D-p-히드록시페닐글리신인 것인 방법.The method of claim 6, wherein the immobilizing enzyme is an immobilized hydantoinase and the N-carbamoyl-D-amino acid is N-carbamoyl-D-p-hydroxyphenylglycine. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930022850A 1993-10-30 1993-10-30 Preparation process of n-carbamoyl-d-amino acid with fixed enzyme KR970002668B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930022850A KR970002668B1 (en) 1993-10-30 1993-10-30 Preparation process of n-carbamoyl-d-amino acid with fixed enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930022850A KR970002668B1 (en) 1993-10-30 1993-10-30 Preparation process of n-carbamoyl-d-amino acid with fixed enzyme

Publications (2)

Publication Number Publication Date
KR950011613A true KR950011613A (en) 1995-05-15
KR970002668B1 KR970002668B1 (en) 1997-03-07

Family

ID=19366990

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019930022850A KR970002668B1 (en) 1993-10-30 1993-10-30 Preparation process of n-carbamoyl-d-amino acid with fixed enzyme

Country Status (1)

Country Link
KR (1) KR970002668B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100324281B1 (en) * 1999-08-31 2002-02-25 서평원 Centralized High Speed Data Processing Module

Also Published As

Publication number Publication date
KR970002668B1 (en) 1997-03-07

Similar Documents

Publication Publication Date Title
Langrand et al. Lipase catalyzed reactions and strategy for alcohol resolution
AR245953A1 (en) Enzymatic peracid bleaching system
KR950008685A (en) Method for preparing immobilized enzyme conjugate and prepared enzyme conjugate
KR920008192A (en) Method for preparing trehalulose and isomaltulose
FI914420A0 (en) A new, particularly thermostable-amylase
EP0126443A3 (en) A process for the selective preparation of microorganisms capable of reproducible reduction of sulfur and use of the microorganism for microbial desulfurization of coal
Qi et al. Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH
GB1457993A (en) Method of bonding enzymes to porous inorganic carriers
KR900018370A (en) Cross-linked glucose isomerase
EP1008647A3 (en) A process for preparing an immobilized enzyme
PT88208A (en) PROCESS FOR THE PREPARATION OF STABILIZED ENZYMES
KR950011613A (en) Method for preparing immobilized enzyme and method for preparing N-carbamoyl-D-amino acid from hydantoin derivative
KR920021707A (en) Method of regulating enzyme activity by rare gas
DK293182A (en) PROCEDURE FOR CARRYING OUT AN ENZYMATIC REACTION
ES543861A0 (en) A PROCEDURE FOR ENZYMATICALLY CONVERTING A POLYSACCHARIDE TO A MONOSACARIDE OR TO ONE OR MORE POLYSACCHARIDES OF LOW MOLECULAR WEIGHT
IE810670L (en) Immobilized lactase
DE3880170D1 (en) SPHERICAL BIO CATALYST AND METHOD FOR THE PRODUCTION THEREOF.
US5846762A (en) Structurally stable gel bead containing entrapped enzyme and method for manufacture thereof
KR900003375A (en) Method for preparing L (-)-tetrahydrofolic acid
CA2277371A1 (en) Siliceous support media for immobilization of enzymes
IT1250017B (en) IMMOBILIZED ENZYME, RELATED PROCEDURE FOR PREPARATION AND USE
KR880003009A (en) How to prepare carnitine
WO1992015674A3 (en) Dna -or rna- modifying enzymes immobilized to supports
RU93008907A (en) METHOD FOR PRODUCING IMMOBILIZED ENZYMES
SU883173A1 (en) Method of preparing immobilized lipase

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
G160 Decision to publish patent application
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20030228

Year of fee payment: 7

LAPS Lapse due to unpaid annual fee