CA2277371A1 - Siliceous support media for immobilization of enzymes - Google Patents

Siliceous support media for immobilization of enzymes Download PDF

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Publication number
CA2277371A1
CA2277371A1 CA002277371A CA2277371A CA2277371A1 CA 2277371 A1 CA2277371 A1 CA 2277371A1 CA 002277371 A CA002277371 A CA 002277371A CA 2277371 A CA2277371 A CA 2277371A CA 2277371 A1 CA2277371 A1 CA 2277371A1
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CA
Canada
Prior art keywords
enzymes
enzyme
immobilization
immobilized
supports
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002277371A
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French (fr)
Other versions
CA2277371C (en
Inventor
Gerard Le Fevre
Bradley A. Saville
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
1484667 ONTARIO Ltd
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Individual
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Publication date
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Priority to CA002277371A priority Critical patent/CA2277371C/en
Publication of CA2277371A1 publication Critical patent/CA2277371A1/en
Application granted granted Critical
Publication of CA2277371C publication Critical patent/CA2277371C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C12P13/225—Tyrosine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/18—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with another compound as one donor, and incorporation of one atom of oxygen (1.14.18)
    • C12Y114/18001—Tyrosinase (1.14.18.1)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A simple, efficient method for immobilization of enzymes on siliceous supports is reported. The support and the enzyme are linked using a polyfunctional cross-linking agent such as glutaraldehyde. Owing to properties peculiar to siliceous supports, the resulting immobilized enzyme is much more stable than enzymes immobilized on other supports. Consequently, much less enzyme is required. The immobilized enzymes generated via this process are ideally suited for continuous enzymatic production of commodity chemicals and pharmaceuticals such as L-DOPA, and for other enzyme-mediated industrial processes.
CA002277371A 1999-07-08 1999-07-08 Siliceous support media for immobilization of enzymes Expired - Fee Related CA2277371C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA002277371A CA2277371C (en) 1999-07-08 1999-07-08 Siliceous support media for immobilization of enzymes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA002277371A CA2277371C (en) 1999-07-08 1999-07-08 Siliceous support media for immobilization of enzymes

Publications (2)

Publication Number Publication Date
CA2277371A1 true CA2277371A1 (en) 2001-01-08
CA2277371C CA2277371C (en) 2005-05-10

Family

ID=4163729

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002277371A Expired - Fee Related CA2277371C (en) 1999-07-08 1999-07-08 Siliceous support media for immobilization of enzymes

Country Status (1)

Country Link
CA (1) CA2277371C (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
WO2014174479A1 (en) * 2013-04-26 2014-10-30 Andrew Desbarats A proppant immobilized enzyme and a viscofied fracture fluid

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019502373A (en) * 2015-12-03 2019-01-31 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ Enzyme delivery system

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007036235A1 (en) 2005-09-30 2007-04-05 Novozymes A/S Immobilization of enzymes
US9303256B2 (en) 2005-09-30 2016-04-05 Novozymes A/S Immobilization of enzymes
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
WO2014174479A1 (en) * 2013-04-26 2014-10-30 Andrew Desbarats A proppant immobilized enzyme and a viscofied fracture fluid
GB2527479A (en) * 2013-04-26 2015-12-23 Andrew Desbarats A proppant immobilized enzyme and a viscofied fracture fluid
US9982524B2 (en) 2013-04-26 2018-05-29 Immortazyme Company Ltd. Method of using viscosified fracture fluid to improve flow of liquids and gases from a subterranean fracture
GB2527479B (en) * 2013-04-26 2020-10-14 Desbarats Andrew A proppant immobilized enzyme and a viscofied fracture fluid
US11105191B2 (en) 2013-04-26 2021-08-31 Immortazyme Company Ltd. Proppant and a viscosified fracture fluid for use in fracturing to extract oil and gas

Also Published As

Publication number Publication date
CA2277371C (en) 2005-05-10

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Effective date: 20160708