KR950008680A - New fermentation broth and method for producing lactic acid using same - Google Patents

New fermentation broth and method for producing lactic acid using same Download PDF

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Publication number
KR950008680A
KR950008680A KR1019930018514A KR930018514A KR950008680A KR 950008680 A KR950008680 A KR 950008680A KR 1019930018514 A KR1019930018514 A KR 1019930018514A KR 930018514 A KR930018514 A KR 930018514A KR 950008680 A KR950008680 A KR 950008680A
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South Korea
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yeast
lactic acid
hydrolyzate
medium
producing
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KR1019930018514A
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Korean (ko)
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KR0122436B1 (en
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조규홍
조윤경
홍승서
박연성
이현수
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김경환
선일포도당 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/245—Lactobacillus casei

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 효모가수분해물을 이용한 신규한 배지에 관한 것이다. 좀더 구체적으로, 본 발명은 효모가수분해물을 이용한 유산발효에 사용되는 배지 및 그를 이용한 경제적인 유산의 제조방법에 관한 것이다. 본 발명에서는 유산균 발효배지 성분으로 널리 이용되는 액상 또는 고체상의 효모균체를 최소한의 가수분해처리를 통해 가공하고 이를 유산균 발효에 배지성분으로 이용함으로써, 맥주산업 등에서 그 부산물로 다량 배출되어 대부분이 버려지는 효모균체를 미생물 배지로 이용하여 경제적으로 유산을 제조하는 방법을 제공한다.The present invention relates to a novel medium using yeast hydrolyzate. More specifically, the present invention relates to a medium used for lactic fermentation using yeast hydrolyzate and a method for producing economical lactic acid using the same. In the present invention, the liquid or solid yeast cells widely used as lactic acid bacterium fermentation broth components are processed through minimal hydrolysis treatment and used as a medium component for lactic acid bacteria fermentation, whereby most of them are discharged as by-products in the beer industry and discarded. It provides a method for producing lactic acid economically using yeast cells as a microbial medium.

Description

신규한 발효용 배지 및 이를 이용한 유산의 제조방법New fermentation broth and method for producing lactic acid using same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (7)

효모균체를 키티나제, 자이몰라제 및 프로테아제로 구성된 그룹으로부터 선택된 1종 이상의 효소를 이용하여 가수분해시켜 제조한 효모가스 분해물.A yeast gas degradation product prepared by hydrolyzing a yeast cell using at least one enzyme selected from the group consisting of chitinase, zymolase and protease. 제1항에 있어서, 가수분해는 효모를 pH 1.5내지 8.4, 온도 33내지 62℃의 범위에서 수행하여 제조한 것을 특징으로 하는 효모가수분해물.The yeast hydrolyzate of claim 1, wherein the hydrolysis is prepared by performing the yeast at a pH of 1.5 to 8.4 and a temperature of 33 to 62 ° C. 효모균체를 산 또는 알칼리를 이용하여 가수분해시켜 제조한 효모가수분해물.Yeast hydrolyzate prepared by hydrolyzing yeast cells with acid or alkali. 제3항에 있어서, 효모균체를 4N 염산에 현탁하여 40내지 80℃에서 1내지 6시간 처리하여 가수분해시켜 제조한 것을 특징으로 하는 효모가수분해물.4. The yeast hydrolyzate of claim 3, wherein the yeast cells are suspended in 4N hydrochloric acid and treated by hydrolysis for 1 to 6 hours at 40 to 80 deg. 다음과 같은 조성을 가지는 발효용 배지:Fermentation medium having the following composition: 상기에서, 효모 가수분해물은 효모균체를 가수분해한 것이며; 염류는 (NH4)2PO4, MgSO4, CH3COONa 및 FeSO4등으로 부터 선택된다.In the above, the yeast hydrolyzate is a hydrolyzed yeast cell; Salts are selected from (NH 4 ) 2 PO 4 , MgSO 4 , CH 3 COONa, FeSO 4 , and the like. 제5항의 발효용 배지를 영양배지로 이용하여 유산생성 미생물을 발효시켜 유산을 제조하는 방법.A method for producing lactic acid by fermenting lactic acid-producing microorganisms using the fermentation medium of claim 5 as a nutrient medium. 제6항에 있어서, 유산생성 미생물은 락토바실러스 카제이 서브스페시스 람노서스(Lactobacillus casei subsp, rhamnosus, KCTC 0084BP)인 것을 특징으로 하는 방법.The method of claim 6, wherein the lactic acid microorganism is Lactobacillus casei subsp, rhamnosus, KCTC 0084BP. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930018514A 1993-09-15 1993-09-15 Method of lactic acid with yeast hydrolyzed material as nitrogen source KR0122436B1 (en)

Priority Applications (1)

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KR1019930018514A KR0122436B1 (en) 1993-09-15 1993-09-15 Method of lactic acid with yeast hydrolyzed material as nitrogen source

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Application Number Priority Date Filing Date Title
KR1019930018514A KR0122436B1 (en) 1993-09-15 1993-09-15 Method of lactic acid with yeast hydrolyzed material as nitrogen source

Publications (2)

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KR950008680A true KR950008680A (en) 1995-04-19
KR0122436B1 KR0122436B1 (en) 1997-11-24

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