KR950008040B1 - Microoganism material producing method for waste water clarification - Google Patents

Abstract

The microorganism preparation method for wastewater treatment consists of (1) mixing culture broth with oil (I) 10-50% and antioxidant (II) 0.1-5 ppm, and (2) drying mixture by heating, freezing, vacuuming or spraying to water content 10-15%. The dried microorganisms are alive till 80 days. Microorganisms are coated by (I), one of corn, soy or ricebran from drying. (II), one of dibutyl hydroxy toluene, butylhydroxy anisole, propylene gallate or erythorbate keeps (I) from oxidising.

Description

Wastewater Treatment Microbial Process

The present invention relates to a method for preparing a wastewater treatment microorganism for preventing long-term and stable preservation of microorganisms of wastewater purification microorganisms, and more specifically, oils and antioxidants in a microbial culture when preparing wastewater purification microorganisms in solid form. It is related to a method for producing a wastewater treatment microorganism that can be added by drying to increase the activation rate and the dry yield, as well as to maintain the viable count for a long time.

Representative methods for treating organic materials in wastewater include biological treatment using a reaction tank (hereinafter referred to as aeration tank), and an active sludge method in which various microorganisms, ie, activated sludge, are decomposed in the aeration tank to decompose organic materials. Is used. The biological treatment method using the aeration tank can decompose various organic substances and has good treatment efficiency, but it requires skilled management skills and the treatment efficiency easily decreases according to load fluctuations. Even if the temperature and organic matter concentration are maintained in a stable range and aeration is performed, not only the risk of killing microorganisms and stopping wastewater treatment but also a problem of bulking phenomenon occurs.

At present, many kinds of microbial agents are used in Korea for the purpose of solving the problems of the biological treatment method using the aeration tank, which is described in Korean Patent Publication No. 91-18406 (Invention) : Polypeptides), No. 90-14258 (Invention name: Method for producing wastewater treatment agent using microorganisms) and Patent Publication No. 91-4083 (Invention name: Method for producing microbial nutrition factor composition for wastewater treatment) It is described.

Wastewater purification microorganisms are mainly used in the solid form, solid form microorganisms are less active than the liquid microbial agent, but excellent in the preservation and stability of microorganisms.

The solid wastewater purification microorganism is prepared by recovering and washing the cultured cells, followed by freeze-drying, vacuum drying or hot air drying by adding an excipient and a drying protective agent to the washed cells.

Since the killing of live bacteria is greatly influenced by the external moisture content, it is important to operate to keep the moisture content in the product as low as possible in the manufacturing process as described above. However, if the product is dried for a long period of time to reduce moisture during the drying process, the drying yield is reduced due to the influence of drying conditions. For example, in terms of manufacturing the product, it is important to shorten the drying time and increase the drying yield even if a certain amount of water is present in the product, while in terms of preservation of the microorganism, it is important to keep the microorganisms a long time by reducing the moisture content as much as possible. Do.

In order to solve the above problems, the present invention provides a method for producing a wastewater treatment microorganism in solid form using oils and antioxidants by maintaining a low moisture content in the product and shortening the drying time to increase the drying yield. There is this.

In order to achieve the above object, the present invention, in the production of solid wastewater treatment microorganisms in order to prevent the death of microorganisms during drying or long-term storage, adding oils and antioxidants to the culture medium, and added to the oils and antioxidants A method for producing a wastewater treatment microbial agent by mixing excipients in a culture solution and drying.

The present invention relates to oils such as corn oil, soybean oil and rice bran oil and dibutyl hydroxytoluene, butyl hydroxyanisloe, gallic acid propylene, erythorbic acid and salts thereof. It is a method of producing a wastewater treatment microbial agent is mixed with an antioxidant such as microbial culture medium and mixed with a solid excipient in the mixed solution so that the moisture content is between 10-15%.

When the wastewater treatment microorganism is prepared in the manner described above, the drying time is shortened, and thus a high drying yield can be obtained. Even though the moisture is 10-15%, the microorganisms are encoded by oils, so the effect of moisture is not affected. It can be stored stably for a long time without receiving. Antioxidants react to oil and water and oxygen during the preservation period of microorganisms, causing rancidity application, which prevents the microorganisms from being killed by the rancidity.

In the wastewater treatment microbial agent of the present invention, the amount of oil is added at 10-50% of the microbial culture, and the amount of antioxidant is added at 0.1-5 ppm of the amount of oil used. In particular, the dry yield and the integrity of 20-50% of the culture medium and 0.2-0.8ppm antioxidant of oil are excellent.

The viable cell count of the microbial agent of the present invention is as follows.

Accurately weigh 1 g of sample, add 9 ml of 0.7% saline solution, and mix well. Take 1 ml of the mixed solution and dilute continuously with 9 ml physiological saline.

Each serial dilution mixture is aseptically taken from each dilution factor and plated on a nutrient agar plate.

The plate is incubated for 3 days in a thermostat.

The colonies (colony) formed on the plate are measured.

The conversion method of viable cell count is 100 × 10 ⁸ / g using the following formula I, if 100 colonies were generated at 1: 1 × 10 ⁸ dilution.

Viable cell count = microbial colony x dilution x 10 (pieces / g) (I)

If the solid wastewater treatment microbial agent is prepared using the oils and antioxidants of the present invention, it is possible to prepare a microbial agent having a high bioburden, and to prevent the killing of microorganisms due to circulation, It is activated immediately upon administration and rapidly decomposes organic materials, making an excellent contribution in the field of wastewater treatment, which is becoming a social problem today.

Example 1

Pseudomonas aeruginosa ATCC 17423 is used as the culture strain.

Inoculate the strain to Tryptic soy medium and incubate at 30 ° C for 1 day.

The viable cell count of the culture solution is measured (2.0 × 10 ¹⁰ cells / ml).

100 ml of the culture solution and 100 g of bran are mixed and sampled for each moisture content while hot-air dried.

While preserving at room temperature, measure the moisture content and viable cell number of each sample as described above, and observe the change of viable cell number according to the storage date.

Table 1 below shows the number of viable cells according to moisture content and the change of viable cell numbers during storage. Here, the microorganism grows with bran as a nutrient source when it is dried during hot air drying, and the moisture content stops and dies when the moisture content is around 25%, and when the hot air drying is finished, the moisture content is 6.9% and the number of live bacteria is 1.7 × 10 ⁹ pcs / g Becomes

In the case of keeping at room temperature for each moisture content and measuring the change of viable cell number, the decay occurred inside the moisture content up to 23.5%, and many other germs grew, and Pseudomonas eroginosa bacteria tended to decrease gradually. The higher the moisture content, the greater the triton fraction of Pseudomonas eroginosa bacteria.

TABLE 1

Example 2

For each 100 ml of the culture solution obtained according to the method of Example 1, the first treatment was performed without any treatment, the second treatment with 20 ml of corn oil, and the third treatment with 20 ml of corn oil and 10 μg of dibutyl hydroxy toluene. do.

Each culture solution treated with the above conditions was mixed with 100 g of bran to obtain a product having a moisture content of 12% by hot air drying.

The viable cell count of each of the above products is measured and stored at room temperature, and the change in viable cell number according to the storage date is observed.

Table 2 below shows the effect on the dry cell number and preservation according to the addition of oil and antioxidants. Here, when treated with corn oil, the number of viable cells after drying is increased compared to the first case without any treatment, and there is no change of viable cell number until 40 days when stored at room temperature. However, after 40 days, the number of viable cells decreases rapidly due to rancidity caused by the combination of oils with air or oxygen.

In the third case in which dibutyl hydroxy toluene, which is a oxidizing agent, is added to prevent rancidity, the number of dry cells does not increase, but there is almost no decrease in viable counts even when stored at room temperature for 80 days.

TABLE 2

Claims (5)

A method for producing a wastewater treatment microorganism, wherein the oil and antioxidant are added to the microbial culture medium during the drying and long-term storage of the microbial agent, and the excipient is mixed with the mixed solution and dried. The wastewater according to claim 1, wherein the oils include corn oil, soybean oil, and rice bran, and the antioxidants include dibutyl hydroxy toluene, butylhydroxy anisole, phosphate propyl, erythorbic acid, and salts thereof. Method for producing a purified microbial agent. The method of claim 1, wherein the oils are 10-15% of the culture, and the antioxidant is 0.1-5 ppm of the oil used, respectively. The method of claim 1, wherein the drying method is hot air drying, freeze drying, vacuum drying, and spray drying. The method of claim 4, wherein the water content of the wastewater treatment microorganism after drying is 10-15%.