KR950004694B1 - Stabilization method of glycyrrhiza uralensis extraction for using cosmetics - Google Patents

Abstract

The flavonoid extract of glycyrrhiza uralensis for using as cosmetic is stabilized by dissolving cosmetic extract contg. flavonoid in at least one or more triglyceride of saturated fatty acid e.g. tri (capryl, capric acid) glycerin, long-chain aliphatic alcohol e.g. myristyl octyl dodecanol, 2-hexyl decanol or 2-octyl dodecanol, and saturated fatty acid ester of low alcohol. The flavonoid of glycyrrhiza uralensis contains liquiritigenin, isoliquiritigenin, liquiritin, neoliquiritin, neoisoliquiritin and liquirazid.

Description

Stabilization Method of Cosmetic Licorice Flavonoid Extracts

The present invention relates to a method for stabilizing flavonoid components of licorice used as a raw material of cosmetics. Specifically, the present invention provides a method for stabilizing flavonoid components of licorice, among flavonoids contained in skin cosmetics and hair cosmetics, which can protect the living body from harmful free radicals.

Herbal medicines containing flavonoids, pollen, etc. have been used to treat various diseases since ancient times, and are known to have a function of restoring capillaries, which are particularly vulnerable with vitamin C.

Licorice (Glycyrrhiza uralensis) is a perennial herb belonging to the family Fabaceae, whose roots are used for medicine. Licorice not peeled on the outer surface is yellowish brown or dark reddish brown with fine lines in length. Peeled licorice is light yellowish and fibrous. It is a good variety that is hard, heavy, low fiber and sweet. Herbal herbal medicine is said to have no poison because it is sweet and flat, and the medicinal odor is written to detoxify all medicine and prevent side effects of the medicine. In addition, it is written that it helps to relieve the bad feelings, to remove the heat frauds of the five intestines, to strengthen the muscles and bones, and to live in the spirit.

Licorice has been so widely used that it is indispensable in oriental medicine. It is also found in the scriptures that the saints of India widely recommended licorice to the public as a tonic, elixir of life, or beauty. In addition, licorice is a universal medicine in China, many people love it.

Egypt's history, written in scribes, says that licorice was used in a drink called Mysace in the Pharaoh period. It is also recorded in the record that Alexander the Great treated licorice as an indispensable bottle and carried it on various expeditions. Like this, licorice has been widely used from the king up to the common people below.

Licorice (Glycyrrhiza uralensis Fischer et. De candolle; Licorice) has been used in the West since the days of Theophrastus in Rome, and Rome describes licorice as Radix Dulcis.

In Asia, it appears that it is already found in the new agricultural herb, and it is estimated that it has been living for about 4,000 years.

Today, it is widely grown in Turkey, Greece, Asia Minor, and the Soviet Union, and Manchurian licorice is grown in the northeastern region of Asia. Licorice has a slightly unusual smell, tastes sweet and residual. Licorice detoxifies toxic substances, so it enhances the function of the liver, and is effective in drug addiction, urticaria, eczema, etc. It is also used in medicines such as Jinhae, expectoration, gastric ulcers, and stomach pain. It is an indispensable potion in oriental medicine because it acts to relieve the poison of all medicines and neutralize them.

Cosmetic makers, on the other hand, use flavonoids in cosmetics as substances that inhibit aging to protect living cells from harmful free radicals. Flavonoids are widely distributed in the plant family and are present in natural plant pigments, particularly in the leaves, flowering tissues, pollen, etc., and often present as glucosides, and also exist as aglycones and methylated derivatives. Currently considered flavonoids in plants have been reported to filter ultraviolet rays and to sulphate by scavenging free radicals and radicals that are harmful to living organisms. Recently, it has been found that the flavonoid component inhibits the production of lipid peroxide, which causes aging, in animal cells.

Flavonoids contained in herbal medicines used in herbal medicine slows the aging process and promotes cell regeneration.

Licorice is divided into three classes. That is, triterpenoid derivatives, flavone analogs, isoflavones. The triterpenoid derivatives include glycyrrhizin and glycyrrhetinic acid, and the prabonoids include liquiritigenin, isoliquiritigenin and liquiritin. Itin, neooliquiritin, neoisoliquiritin and liqu irazid are contained and these flanoids have antispasmic, antiinflammatory and anti-rash It has an antieruption effect.

The method for obtaining the licorice flavonoid of the present invention is as follows.

1 kilogram of herbal medicine licorice is added to an extractor equipped with a reflux condenser, and 10-15 liters of distilled water or hydrous alcohol is added and left overnight. The extract is filtered by hot or cold for 1 to 10 days and then filtered. The filtrate was concentrated in vacuo to give a syrupy residue. 10 to 15 liters of methanol is added, warmed and dissolved, and left to stand for precipitation. After removing the precipitate, the filtrate is concentrated in vacuo to obtain a syrupy substance. This was dissolved in methanol again and Sephadex LH-20 chromatography gave 180 grams of licorice flavonoids of the present invention.

Hereinafter, the term 'licorice flavonoid' used in the present invention is obtained by concentrating an extract filtrate obtained by extracting licorice with distilled water or hydrous alcohol and dissolving it in methanol, for example, by purification by column chromatography. Cosmetics Containing Liquiriti Genin, Isoliquiritigenin, Liquiritin, Neoliquiritin, Neoisoliquiritin, Liquirazid Means licorice flavonoid extract.

Licorice flavonoids thus obtained may be used as raw materials for cosmetics, but in order to be effectively used in skin cosmetics or makeup cosmetics, it is more convenient to dissolve them in a suitable solvent used in cosmetics. However, when the licorice flavonoids of the present invention are dissolved in ethanol or a mixture of ethanol and water as a raw material, there is a problem that the content of the active ingredient gradually disappears and discolors occur over time due to daylight or inferiority. Eventually, the value of the raw material is lost.

The inventors of the present invention dissolved the licorice flavonoids of the present invention using various solvents used in cosmetics and experimentally measuring the stability against sunlight and temperature, and the licorice flavonoids of the present invention were glycerides, long-chain aliphatic alcohols of saturated fatty acids, or When dissolved in esters of lower alcohols and saturated fatty acids, they were found to be stored stably without oxidative degradation and discoloration against aging changes such as sunlight and temperature.

The involvement of hydroxy radicals in the oxidative damage of flavonoids also plays a role in the formation of hydrogen peroxide and superoxide in the presence of metals, where the superoxide also plays a role in oxidative damage. Therefore, in order to suppress oxidative decomposition of flavonoids, it is necessary to prevent radical formation or to block radical formation by blocking oxygen.

Conventionally, in order to prevent oxidative decomposition of flavonoids, a method of preventing contact with oxygen by adding another antioxidant to the raw material itself or by filling inert gas such as nitrogen is used. However, this method eventually increases the price of raw materials and is not economical. In addition, there are various troubles in the management of raw materials.

The present inventors studied a method of stably storing flavonoids of the present invention, that is, licorice flavonoids, against oxidative degradation using various raw materials used in cosmetics as solvents, and as a result, triglycerides of saturated fatty acids, long-chain aliphatic alcohols, or When dissolved in esters of lower alcohols and powa fatty acids, it has been found that it can be stored stably, especially with respect to changes over time. A small amount of phytic acid, which synergistically stabilizes the licorice flavonoids of the present invention in a liquid state The invention has been completed.

Specifically, triglycerides composed of saturated fatty acid esters such as tri (capryl, capric acid) glycerin and glyceryl trioctanoate may be used for storing licorice flavonoids of the present invention in a liquid state. Long-chain fatty acid alcohols such as myristyl octyldodecanol, 2-octyldodecanol and 2-hexyldecanol, cetyl-2-ethylhexanoate and myristinic acid dipropyl, dimethyloctanoate hexyldecyl, lauric acid Saturated fatty acid esters of lower alcohols such as hexyl, octylate acid octyldodecyl, isopropyl palmitate, sechioctanoate and diisostearyl maleate. The ratio of flavonoids to said material is suitably 0.01-10% by weight. A certain amount of licorice prabonoids was dissolved in these raw materials to be in a liquid state, and then put into daylight and constant temperature incubation, and the contents of the active ingredients and the degree of discoloration were compared.

Hereinafter will be described in detail through an embodiment of the present invention.

Example 1

Flavonoids obtained from licorice of the present invention were dissolved in tri (capryl, capric acid) glycerin (trade name: Neobee M-5) in a ratio of 0.1% by weight and 1% by weight, and 0.001% of phytic acid was added thereto, followed by stirring. The contents of the active ingredients and the degree of discoloration were measured at 5 days intervals by keeping in a thermostat and daylight (summer season). For control, the licorice flavonoids in 95% ethanol were compared after storage in a thermostat and sunlight.

Example 2

Flavonoids obtained from the licorice of the present invention were dissolved in isopropyl myristin at a weight ratio of 0.1% and 1%, and measured in the same manner as in Example 1 below.

Example 3

Flavonoids obtained from licorice of the present invention were dissolved in cetyl 2-ethylhexanoate at a weight ratio of 0.1% and 1%, and measured in the same manner as in Example 1 below.

Example 4

The flavonoids obtained from the licorice of the present invention were dissolved in myristyloctyldodecanol in a weight ratio of 0.1% and 1%, and measured in the same manner as in Example 1 below.

Example 5

Flavonoids obtained from the licorice of the present invention were dissolved in 2-octyldodecanol at a weight ratio of 0.1% and 1%, and specified in the same manner as in Example 1 below.

In each example, the content of the active ingredient was measured by absorbance at 361 nm, which is the λmax of the licorice flavonoid of the present invention, in a UV-VIS absorbance meter to determine the relative percentage of contents.

Samples were used for the experiment after a certain period of time respectively in daylight (summer season) and constant temperature (45 ℃). Tables 1 and 2 show the relative percentages of liquid licorice flavonoids obtained from high-performance liquid chromatography at daylight and constant temperature. Each measurement was performed three times and averaged.

On the other hand, the degree of discoloration was compared with the ΔE ^* value using a color difference spectrometer (device name: Ultrascan Spectrophotometer, manufacturer: Hunter Associates Laboratory, Inc.).

Where ΔE ^* = [(ΔL ^* ) ² + (Δa ^* ) ² + (Δb ^* ) ² ] ^1/2 . The measurement was performed 3 times and the average value was calculated | required. Table 3 shows the results.

As shown in Table 1, Example 1-5 of the present invention showed more than twice the amount of the active ingredient after 30 days in daylight and constant temperature compared to the control example.

In Table 3, the color change of the licorice flavonoid solution with time was shown to increase continuously until 30 days in the case of the control example, but in the case of Examples 1-5 of the present invention 10 days regardless of the concentration It can be seen that until the increase slightly until there is no change. The degree of color change can also be seen that the case of Example 1-5 of the present invention is more than two times more stable than the control example.

TABLE 1

TABLE 2

TABLE 3

Claims (6)

A flavonoid-containing cosmetic extract obtained from licorice is dissolved in triglycerides of saturated fatty acids, long-chain aliphatic alcohols, or saturated fatty acid esters of lower alcohols, or a method of stabilizing licorice flavonoids for cosmetics. The triglyceride of saturated fatty acid is tri (capryl, capric acid) glycerin, glyceryltrioctanoate, and the long chain aliphatic alcohol is myristyloctyldodecanol, 2-hexyldecanol or 2- Octyldodecanol, and saturated fatty acid esters of lower alcohols are cetyl 2-ethylhexanoate, dimethyloctanoate hexyldecyl, lauric acid hexyl, octystinate octyldodecyl, isopropyl palmitate, cecyloctanoate, di Isostearyl maleate or isopropyl acid isopropyl. The phytic acid is contained in a solution obtained by dissolving a flavonoid-containing cosmetic extract obtained from the licorice in one or two or more saturated fatty acid triglycerides, long-chain aliphatic alcohols, or saturated alcohols of lower alcohols. The method characterized in that the addition of more. The method according to claim 1, wherein the dissolution ratio of licorice flavonoids to triglycerides of saturated fatty acids, long-chain aliphatic alcohols, or one or two or more kinds of saturated fatty acid esters of lower alcohols is 0.01 to 10% by weight. 4. The method of claim 3, wherein the phytic acid is added at 0.001 to 0.01 wt% based on the total weight of the solution. The method of claim 1, wherein the cosmetic licorice prabonoid is liquritigenin (liq uiritigenin), isoliquiritigenin (liquiritin), liquiritin (neoliquiritin), neoisoriquiritin (neoisoliquiritin) and liquirazid.