KR950003315A - 저분자량-우로가스트론의 정제방법 - Google Patents
저분자량-우로가스트론의 정제방법 Download PDFInfo
- Publication number
- KR950003315A KR950003315A KR1019930014842A KR930014842A KR950003315A KR 950003315 A KR950003315 A KR 950003315A KR 1019930014842 A KR1019930014842 A KR 1019930014842A KR 930014842 A KR930014842 A KR 930014842A KR 950003315 A KR950003315 A KR 950003315A
- Authority
- KR
- South Korea
- Prior art keywords
- urogastron
- molecular weight
- urine
- purification method
- hmw
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 13
- 238000000746 purification Methods 0.000 title claims abstract 4
- 210000002700 urine Anatomy 0.000 claims abstract 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract 4
- 230000000767 anti-ulcer Effects 0.000 claims abstract 4
- 230000027119 gastric acid secretion Effects 0.000 claims abstract 4
- 230000000694 effects Effects 0.000 claims abstract 3
- 238000000605 extraction Methods 0.000 claims abstract 3
- 238000001556 precipitation Methods 0.000 claims abstract 3
- 238000000108 ultra-filtration Methods 0.000 claims abstract 3
- 241001465754 Metazoa Species 0.000 claims abstract 2
- 239000003463 adsorbent Substances 0.000 claims abstract 2
- 230000008021 deposition Effects 0.000 claims abstract 2
- 238000001179 sorption measurement Methods 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 2
- 238000001914 filtration Methods 0.000 claims 1
- 230000002401 inhibitory effect Effects 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 238000004440 column chromatography Methods 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Water Supply & Treatment (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
본 발명은 뇨중에 존재하는 위산분비억제 효과 및 항궤양작용이 있는 분자량 30,000이상의 고분자량-우로가스트론을 정제하는 방법에 관한 것이다.
특히, 본 발명은 종래의 정제방법에서 다단계의 컬럼크로마토그래피공정을 거쳐 제조함으로 인하여 과정이 복잡고, 우로가스트론의 활성이 매우 낮다는 문제점을 개선하기 위하여, 사람이나 동물의 뇨를 흡착제를 사용한 흡착, 에탄올-분별침전, 추출, 한외여과 및 아세톤 침적단계를 순차적으로 수행함으로써 컬럼 크로마토그래피 공정을 간단한 공정으로 정제하는 방법에 관한 것이다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (4)
- 사람 또는 동물의 뇨를 흡착제를 사용한 흡착, 에탄올 분별침전, pH 8.0-10.0에서 추출, 한외여과, 아세톤 침적을 순차적으로 행하는 것을 특징으로 하는 위산 분비억제 및 항궤양 작용을 갖는 고분자량-우로가스트론(HMW-UG)의 정제방법.
- 제1항에 있어서, 한외여과시 여과막이 분자량분획(Molecular cut off)30,000의 여과막인 것을 특징으로 하는 위산분비억제 및 항궤양 작용을 갖는 고분자량-우로가스트론(HMW-UG)의 정제방법.
- 제1항 또는 제2항에 있어서, 사람의 뇨가 임신부뇨임을 특징으로 하는 위산 분비억제 및 항궤양 작용을 갖는 고분자량-우로가스트론(HMW-UG)의 정제방법.
- 제1항에 있어서, pH 8.0-10.0에서 추출후, pH 4.0-5.0에서의 침전제거공정을 더욱 포함하는 것을 특징으로 하는 위산분비억제 및 항궤양 작용을 갖는 고분자량-우로가스트론(HMW-UG)의 정제방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930014842A KR0137855B1 (ko) | 1993-07-31 | 1993-07-31 | 저분자량-우로가스트론의 정제방법 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930014842A KR0137855B1 (ko) | 1993-07-31 | 1993-07-31 | 저분자량-우로가스트론의 정제방법 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR950003315A true KR950003315A (ko) | 1995-02-16 |
| KR0137855B1 KR0137855B1 (ko) | 1998-04-30 |
Family
ID=19360560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930014842A KR0137855B1 (ko) | 1993-07-31 | 1993-07-31 | 저분자량-우로가스트론의 정제방법 |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR0137855B1 (ko) |
-
1993
- 1993-07-31 KR KR1019930014842A patent/KR0137855B1/ko not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR0137855B1 (ko) | 1998-04-30 |
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|---|---|---|---|
| A201 | Request for examination | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant | ||
| FPAY | Annual fee payment |
Payment date: 20030204 Year of fee payment: 6 |
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| LAPS | Lapse due to unpaid annual fee |