KR930019822A - 가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도 - Google Patents

가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도 Download PDF

Info

Publication number
KR930019822A
KR930019822A KR1019930003565A KR930003565A KR930019822A KR 930019822 A KR930019822 A KR 930019822A KR 1019930003565 A KR1019930003565 A KR 1019930003565A KR 930003565 A KR930003565 A KR 930003565A KR 930019822 A KR930019822 A KR 930019822A
Authority
KR
South Korea
Prior art keywords
prv
antisense
polynucleotide
gii
site
Prior art date
Application number
KR1019930003565A
Other languages
English (en)
Inventor
스프링어 볼프강
바움가르텐 외르그
크레트쉬머 악셀
퀄블 하인쯔
뢰베르딩 안토니우스
스트루베 발터
타인 페테르
Original Assignee
에론스트 로이터, 클라우스 대너
바이엘 악티엔게젤샤프트
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에론스트 로이터, 클라우스 대너, 바이엘 악티엔게젤샤프트 filed Critical 에론스트 로이터, 클라우스 대너
Publication of KR930019822A publication Critical patent/KR930019822A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1133—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against herpetoviridae, e.g. HSV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
    • C12N2710/16722—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

본 발명은 안티센스 방향으로 가광견병 바이러스 서열, 특히 PRV의 gII 부위로 부터의 서열을 함유하는 폴리뉴클레오티드, 그의 제조방법 및 그의 용도에 관한 것이다.

Description

가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 PRV의 gII 부위를 포함하는 PRV의 제한지도의 한 부분 및 예로써 인용된 안티센스 작제물의 위치를 나타낸다.
제2도는 벡터의 SV40초기 프로모터에 대해 안티센스 방향으로 180bp길이의 PRV-gII단편(서열범위 879-1058)을 갖는 pBEH의 벡터지도를 나타낸다.
제3도는 진핵성 발현벡터의 프로모터에 대해 안티센스 방향으로, 진핵세포내에서 PRV에 대한 고도의 내성을 야기시키는 PRV gII 부위에서 유래한 180bp 길이의 DNA단편(서열범위 879-1058)의 뉴클레오티드 염기서열을 나타낸다.

Claims (18)

  1. 프로모터 서열에 대해 안티센스 방향으로 위치하는 PRV 유전자 서열을 함유함을 특징으로 하는 안티센스 DNA 폴리뉴클레오티드.
  2. 프로모터 서열에 대해 안티센스 방향으로 위치하는 것으로 PRV 의 gII 부위로부터 유래한 유전자 서열을 함유함을 특징으로 하는 안티센스 DNA 폴리뉴클레오티드.
  3. 프로모터 서열에 대해 안티센스 방향으로 위치하는 것으로, PRV의 gII 부위의 뉴클레오티드 서열 879-1058에 상응하는 180bp 크기의 부위를 함유함을 특징으로 하는 안티센스 DNA 폴리뉴클레오티드.
  4. PRV의 유전자 서열을 함유함을 특징으로 하는 안티센스 RNA 폴리뉴클레오티드.
  5. PRV의 gII 부위로 부터 유리한 유전자 서열을 함유함을 특징으로 하는 안티센스 RNA 폴리뉴클레오티드.
  6. PRV의 gII 부위d의 뉴클레오티드 서열 879-1058에 상응하는 180bp 크기의 부위를 함유함을 특징으로 하는 안티센스 RNA 폴리 뉴클레오티드.
  7. PRV의 바이러스성 RNA에 대해 상보적인 PRV의 유전자 서열을 함유함을 특징으로 하는 안티센스 올리고뉴클레오티드.
  8. PRV의 바이러스성 gII RNA에 대해 상보적인 PRV gII부위의 유전자 서열을 함유함을 특징으로 하는 안티센스 올리고뉴클레오티드.
  9. 바이러스성 gII RNA에 대해 상보적인, PRV의 gII부위로 부터 유래한 180bp 크기의 부위(879-1058)로부터의 유전자 서열을 함유함을 특징으로 하는 안티센스 올리고뉴클레오티드.
  10. 프로모터 서열에 대해 안티센스 방향으로 PRV로 부터의 폴리뉴클레오티드를 함유하는 발현 벡터 작제물.
  11. 프로모터 서열에 대해 안티센스 방향으로 PRV의 gII 부위로 부터의 폴리뉴클레오티드를 함유하는 발현 벡터 작제물.
  12. 프로모터 서열에 대해 안티센스 방향으로 PRV의 gII 부위로 부터의 180bp 폴리뉴클레오티드(879-1058)을 함유하고 있는 발현 벡터 작제물.
  13. 제1항 내지 6항중의 어느 하나에 따르는 안티센스 폴리 뉴클레오티드를 함유하는 세포 및 그의 자손.
  14. 제1항 내지 6항 및 제10항 내지 12항중의 어느 하나에 따르는 안티센스 폴리뉴클레오티드를 세포내에 함유하는 돼지.
  15. 제7항 내지 9항중의 어느 하나에 따르는 안티센스 올리고 뉴클레오티드를 세포내에 함유하는 돼지.
  16. 제1항 내지 6항 및 제10항 내지 12항중의 어느 하나에 따르는 폴리뉴클레오티드를 바이러스-감수성 세포내에 이입시키고, 바이러스성 RNA에 대해 상보적인 RNA를 형성시킴으로써 PRV의 바이러스성 복제를 방지함을 특징으로 하여 PRV-내성세포를 제조하는 방법.
  17. 제7항 내지 9항중의 어느 하나에 따르는 안티센스 올리고 뉴클레오티드를 동물에게 투여함을 특징으로 하여 PRV 감염을 억제 시키는 방법.
  18. 형질 변형된 PRV-내성 동물의 생산에 적합한 세포내에 이입시키기 위한, 제1항 내지 6항 및 제10항 내지 12항중의 어느 하나에 따르는 폴리뉴클레오티드의 용도.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019930003565A 1992-03-13 1993-03-10 가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도 KR930019822A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP4208107.6 1992-03-13
DE4208107A DE4208107A1 (de) 1992-03-13 1992-03-13 Pseudorabies-virus (prv)-polynukleotide und ihre verwendung zur herstellung von virusresistenten eukaryotischen zellen

Publications (1)

Publication Number Publication Date
KR930019822A true KR930019822A (ko) 1993-10-19

Family

ID=6454031

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019930003565A KR930019822A (ko) 1992-03-13 1993-03-10 가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도

Country Status (6)

Country Link
US (1) US5798265A (ko)
EP (1) EP0560156A2 (ko)
JP (1) JPH06303979A (ko)
KR (1) KR930019822A (ko)
AU (1) AU662040B2 (ko)
DE (1) DE4208107A1 (ko)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2283293A1 (en) * 1997-03-11 1998-09-17 Yale University Method to diagnose and treat pathological conditions resulting from deficient ion transport such as pseudohypoaldosteronism type-1
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
DK1624060T3 (da) 1998-03-20 2012-04-10 Commw Scient Ind Res Org Kontrol af genekspression
US6068974A (en) * 1998-04-29 2000-05-30 Klann; Richard Chris Specific, highly sensitive, nested PCR detection scheme for the pseudorabies virus
WO2000044914A1 (en) 1999-01-28 2000-08-03 Medical College Of Georgia Research Institute, Inc. Composition and method for in vivo and in vitro attenuation of gene expression using double stranded rna
CN1375004A (zh) * 1999-04-21 2002-10-16 惠氏公司 抑制多核苷酸序列的功能的方法和组合物
US20040138168A1 (en) * 1999-04-21 2004-07-15 Wyeth Methods and compositions for inhibiting the function of polynucleotide sequences
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
WO2001070949A1 (en) * 2000-03-17 2001-09-27 Benitec Australia Ltd Genetic silencing
US20020132257A1 (en) * 2001-01-31 2002-09-19 Tony Giordano Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
WO2004106517A1 (en) * 2003-06-03 2004-12-09 Benitec Australia Limited Double-stranded nucleic acid
DE202005004135U1 (de) * 2005-03-11 2005-05-19 Klocke Verpackungs-Service Gmbh Mehrkomponentenverpackung mit Applikator
SE540582C2 (en) 2016-12-22 2018-10-02 Scandibio Therapeutics Ab Substances for treatment of fatty liver-related conditions

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) * 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA

Also Published As

Publication number Publication date
AU3402593A (en) 1993-09-16
EP0560156A2 (de) 1993-09-15
AU662040B2 (en) 1995-08-17
US5798265A (en) 1998-08-25
JPH06303979A (ja) 1994-11-01
DE4208107A1 (de) 1993-09-16
EP0560156A3 (ko) 1995-03-01

Similar Documents

Publication Publication Date Title
Robertson et al. Polyadenylation sites for influenza virus mRNA
KR930019822A (ko) 가광견병 바이러스 폴리뉴클레오티드 및 바이러스-내성 진핵세포의 제조를 위한 그의 용도
Barker et al. Nucieotide sequence of the maize transposable element Mul
McGhee et al. A 200 base pair region at the 5′ end of the chicken adult β-globin gene is accessible to nuclease digestion
Gagliardi et al. Polyadenylation accelerates the degradation of the mitochondrial mRNA associated with cytoplasmic male sterility in sunflower
KR960705036A (ko) Dna 바이러스 리보자임
CN103343120A (zh) 一种小麦基因组定点改造方法
ES2094744T3 (es) Manipulaciones geneticas con el adn recombinante que contiene secuencias derivadas de los virus arn.
PT100472A (pt) Sequencias genomicas do virus da hepatite c e composicoes farmaceuticas que as contem, para diagnostico e terapeutica da hepatite c
Pottmeyer et al. T4 endonuclease VII resolves cruciform DNA with nick and counter-nick and its activity is directed by local nucleotide sequence
Meier et al. Sites of copy choice replication involved in generation of vesicular stomatitis virus defective-interfering particle RNAs
ES2076143T3 (es) Plantas resistentes a virus que tienen arn antisentido.
Steege et al. Euglena gracilis chloroplast small subunit rRNA. Sequence and base pairing potential of the 3'terminus, cleavage by colicin E3.
Hess et al. End-to-end transcription of an Alu family repeat: a new type of polymerase-III-dependent terminator and its evolutionary implication
Demers et al. The L1 family of long interspersed repetitive DNA in rabbits: sequence, copy number, conserved open reading frames, and similarity to keratin
Rezaian et al. Anti-sense regions in satellite RNA of cucumber mosaic virus form stable complexes with the viral coat protein gene
Ohba et al. Induction of DNA replication by transcription in the region upstream of the human c-myc gene in a model replication system
EA199900144A1 (ru) Способ индуцирования вирусной устойчивости в растении
Grunstein et al. The histone H4 gene of Strongylocentrotus purpuratus: DNA and mRNA sequences at the 5′ end
Ghidelli et al. High mobility group proteins cHMG 1a, cHMG 1b, and cHMGI are distinctly distributed in chromosomes and differentially expressed during ecdysone dependent cell differentiation
ATE337404T1 (de) Zur verleihung viraler resistenz an pflanzen geeignetes polyribozym und dieses polyribozym produzierende resistente pflanzen
Resnekov et al. Transcription termination in animal viruses and cells
Schmitt et al. Characterization of cloned sequences complementary to F9 cell double-stranded RNA and their expression during differentiation
Miyano et al. A common feature shared by bent DNA structures locating in the eukaryotic promoter region
KR970001543A (ko) 신규 프로모터 및 이를 이용한 유전자 발현 방법

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application