KR927003795A - Megakaryocytes and platelet growth, methods of preparation and compositions comprising them - Google Patents

Megakaryocytes and platelet growth, methods of preparation and compositions comprising them Download PDF

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Publication number
KR927003795A
KR927003795A KR1019920701338A KR920701338A KR927003795A KR 927003795 A KR927003795 A KR 927003795A KR 1019920701338 A KR1019920701338 A KR 1019920701338A KR 920701338 A KR920701338 A KR 920701338A KR 927003795 A KR927003795 A KR 927003795A
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South Korea
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cells
megakaryocyte
angiogenesis
cell
megakaryocytes
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KR1019920701338A
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Korean (ko)
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티드마쉬 죠오지
씨. 영 쥬디
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시스테믹스, 인코포레이티드
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Publication of KR927003795A publication Critical patent/KR927003795A/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C12N2501/91—Heparin

Abstract

내용 없음No content

Description

거핵세포 및 혈소판 성장, 제조방법 및 이것들을 포함하는 조성물Megakaryocytes and platelet growth, methods of preparation and compositions comprising them

[도면의 간단한 설명][Brief Description of Drawings]

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (14)

사람 혈장을 포함하고 있는 영양 배지에서 거핵세포 성장을 지지하는 조건 및/또는 섬유아세포 성장을 억제하는 조건하에서, 사람 조혈 선구세포 또는 그것의 거핵세포 선구세포가 풍부한 분획을 배양하는 것으로 이루어지는, 거핵 세포 신생 제조 방법.Megakaryocytes, comprising culturing a fraction rich in human hematopoietic progenitor cells or their megakaryocyte progenitor cells under conditions that support megakaryocyte growth and / or inhibit fibroblast growth in a nutrient medium containing human plasma New manufacturing method. 제1항에 있어서, 상기 배양이 섬유아세포 성장을 지지하는 코팅이 결핍되어 있는 용기에서 이루어지는 것을 특징으로 하는 방법.The method of claim 1, wherein the culture is in a container lacking a coating that supports fibroblast growth. 제1항에 있어서, 상기 배지가 헤파린과 우태아 혈청을 포함하는 것을 특징으로 하는 방법.The method of claim 1, wherein the medium comprises heparin and fetal bovine serum. 제1항에 있어서, 상기 배양 배지가 약 20%의 사람 혈장 또는 우태아 혈청을 포함하는 것을 특징으로 하는 방법.The method of claim 1, wherein the culture medium comprises about 20% human plasma or fetal bovine serum. 제1항에 있어서, 상기 배지된 보충용 거핵세포 성장 인자를 포함하는 것을 특징으로 하는 방법.The method of claim 1, comprising the supplemented megakaryocyte growth factor in the culture medium. 제7항에 있어서, 상기 사람 조혈 선구세포가 CD34+, -41+, -14- 분획 조혈세포 분획이 풍부한 것임을 특징으로 하는 방법.8. The method of claim 7, wherein said human hematopoietic progenitor cells are enriched in CD34 +, -41+, -14- fraction hematopoietic cell fractions. CD34 및 41을 가지고 있으며, CD14가 결핍되어 있는 세포가 풍부한 조혈세포 조성물.Cell-rich hematopoietic cell composition having CD34 and 41 and lacking CD14. 제8항에 있어서, 상기 세포가 CD33을 가지고 있는 세포가 풍부한 것을 특징으로 하는 세포조성물.9. A cell composition according to claim 8, wherein said cell is enriched in cells having CD33. 거핵세포 신생으로 진행되는 세포를 최소한 50% 포함하며, 세포의 최소한 10%는 거핵세포 선구세포인 것을 특징으로 하는 사람 세포의 세포조성물.A cell composition of human cells comprising at least 50% of the cells that progress to megakaryocyte angiogenesis, wherein at least 10% of the cells are megakaryocyte progenitor cells. 제9항에 있어서, 상기 세포의 최소한 90%가 거핵세포 선구세포인 것을 특징으로 하는 세포조성물.10. The cell composition of claim 9, wherein at least 90% of the cells are megakaryocyte progenitor cells. 혈소판 이외의 세포의 최소한 50중량%가 거핵세포로 진행되는 특정 선구세포이거나 거핵세포인 것을 특징으로 하는 액체 배지중의 세포조성물.A cell composition in a liquid medium, characterized in that at least 50% by weight of cells other than platelets are specific progenitor cells or megakaryocytes that progress to megakaryocytes. 거핵세포 신생을 조절하는 인자의 측정 방법으로서, 혈청, 관심의 인자 및 IL-1, -3, -6 또는 에리트로포이에틴인 사이토킨을 최소한 하나 포함하고 있는 액체 또는 반고체 영양 배지에 골수 또는 연막으로 부터의 백혈구를 시이딩하는 단계; 그리고 관심의 인자의 부재하에 동일한 조건에서 성장하는 세포와 비교하여 약 5일 이상 더 성장하는 거핵세포의 수를 측정하는 단계로 이루어지는 방법.A method of measuring factors regulating megakaryocyte angiogenesis, the method comprising measuring serum, factors of interest, and bone marrow or smokescreens in a liquid or semisolid nutritional medium containing at least one cytokine that is IL-1, -3, -6 or erythropoietin. Seeding white blood cells; And measuring the number of megakaryocytes growing at least about 5 days compared to cells growing under the same conditions in the absence of a factor of interest. 세포 군집에서 거핵세포 신생 수준을 측정하는 방법으로서, 상기 세포를 용해시켜 상청액을 제조하는 단계; 상기 상청액에서 다른 단백질들로 부터 src 키나아제를 분리하는 단계; 그리고 상기세포 군집의 거핵세포 군집의 척도로서 src 키나아제 활성의 양을 측정하는 단계로 이루어지는 방법.A method of measuring megakaryocyte angiogenesis levels in a cell population, comprising: lysing the cells to prepare a supernatant; Separating src kinase from other proteins in the supernatant; And measuring the amount of src kinase activity as a measure of the megakaryocyte population of said cell population. 혈장에 존재하고, 혈장내에서 56℃에서 최소한 2주동안 안정하며, 헤파린-세파로오스에 결합하여 헤파린 함유 IMDM 배지로 용출될 수 있고, 거핵 세포 신생을 지지하며, 이것의 부재시에 거핵세포 신생이 감소되는 것을 특징으로 하는, 거핵세포 신생을 지지할 수 있는 인자.Present in plasma, stable at 56 ° C. in plasma for at least 2 weeks, can bind to heparin-sepharose and elute with heparin-containing IMDM medium, support megakaryocyte angiogenesis, in the absence of megakaryocytic angiogenesis A factor capable of supporting megakaryocyte angiogenesis, characterized by being reduced. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920701338A 1990-10-05 1991-10-04 Megakaryocytes and platelet growth, methods of preparation and compositions comprising them KR927003795A (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US59368890A 1990-10-05 1990-10-05
US593,688 1990-10-05
US68266691A 1991-04-09 1991-04-09
US682,666 1991-04-09
PCT/US1991/007159 WO1992006178A1 (en) 1990-10-05 1991-10-04 Megakaryocyte and platelet growth, production and composition

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EP (1) EP0504361A4 (en)
JP (1) JPH05502385A (en)
KR (1) KR927003795A (en)
AU (1) AU639378B2 (en)
CA (1) CA2069879A1 (en)
WO (1) WO1992006178A1 (en)

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FR2691066B1 (en) * 1992-05-15 1995-06-09 Sanofi Elf USE OF EXOGENOUS GLYCOSAMINOGLYCANS, THEIR ANALOGS AS WELL AS THEIR FRAGMENTS, FRACTIONS AND DERIVATIVES, FOR THE PREPARATION OF MEDICAMENTS FOR THE TREATMENT OF THROMBOPENIA.
WO1994013306A1 (en) * 1992-12-11 1994-06-23 Systemix, Inc. Megakaryocyte and platelet growth, production and composition
US5846529A (en) * 1993-08-23 1998-12-08 Nexell Therapeutics, Inc. Infusion of neutrophil precursors for treatment of neutropenia
US6037174A (en) * 1993-08-23 2000-03-14 Nexell Therapeutics, Inc. Preparation of serum-free suspensions of human hematopoietic cells or precursor cells
JPH09505462A (en) * 1993-08-23 1997-06-03 バクスター、インターナショナル、インコーポレイテッド In vitro proliferation of neutrophil and megakaryocyte progenitor cells in serum-free medium.
US5795569A (en) * 1994-03-31 1998-08-18 Amgen Inc. Mono-pegylated proteins that stimulate megakaryocyte growth and differentiation
CZ288926B6 (en) * 1994-03-31 2001-09-12 Amgen Inc. MGDF derivative, process of its preparation and pharmaceutical preparation in it is comprised
JP4724836B2 (en) * 2005-04-05 2011-07-13 国立大学法人帯広畜産大学 Cell release method, cell release solution, cell culture method, cell culture solution, cell solution, cell solution preparation, cell fixing method and cell fixing solution
JP2008109866A (en) * 2006-10-30 2008-05-15 Jms Co Ltd Medium additive, medium containing the same, and method for culturing cell using medium
PT2539441T (en) * 2010-02-22 2017-01-05 Assist Publique Hôpitaux De Paris Cell culture medium for the growth and differentiation of cells of the hematopoietic lineage

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US4350687A (en) * 1980-02-10 1982-09-21 Research Corporation Platelet derived cell growth factor
US4894440A (en) * 1986-09-17 1990-01-16 Massachusetts Institute Of Technology Method of isolating megakaryocyte stimulatory factor
JP2823567B2 (en) * 1988-09-06 1998-11-11 生化学工業株式会社 Epithelial tumor cell line

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EP0504361A1 (en) 1992-09-23
JPH05502385A (en) 1993-04-28
EP0504361A4 (en) 1994-08-17
AU8714491A (en) 1992-04-28
CA2069879A1 (en) 1992-04-06
AU639378B2 (en) 1993-07-22

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