KR920019810A - 섬유아세포 성장 인자에 대해 친화성을 갖는 올리고사카라이드 및 이의 제조방법 - Google Patents

섬유아세포 성장 인자에 대해 친화성을 갖는 올리고사카라이드 및 이의 제조방법 Download PDF

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KR920019810A
KR920019810A KR1019920006345A KR920006345A KR920019810A KR 920019810 A KR920019810 A KR 920019810A KR 1019920006345 A KR1019920006345 A KR 1019920006345A KR 920006345 A KR920006345 A KR 920006345A KR 920019810 A KR920019810 A KR 920019810A
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growth factor
fibroblast growth
sulfate
affinity
carrier
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히토코 하부치
사카루 스즈키
고지 기마타
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원본 미기재
세이카가쿠 고교 가부시키가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H5/00—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium
    • C07H5/08—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to sulfur, selenium or tellurium
    • C07H5/10—Compounds containing saccharide radicals in which the hetero bonds to oxygen have been replaced by the same number of hetero bonds to halogen, nitrogen, sulfur, selenium, or tellurium to sulfur, selenium or tellurium to sulfur
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0075—Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
    • C08B37/0078—Degradation products
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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Abstract

내용 없음

Description

섬유아세포 성장 인자에 대해 친화성을 갖는 올리고사카라이드 및 이의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 bFGF에 대해 고친화력을 갖는 헤파란 설페이트 샘플의 bFGF-세파로즈 칼럼(Sepharose column)으로부터의 용출 패턴을 도시하며, 여기서는 소의 대동맥에서 유래된 헤파란 설페이트이며 ○는 마우스의 EHS 종양에서 유래된 헤파란 설페이트이고 ;
제2도는 bFGF에 대해 친화력을 갖는 올리고사카라이드 샘플의 bFGF-.세파로즈 칼럼으로부터의 용출 패턴을 도시하며 ;
제3도는 FGF에 대해 친화력을 갖는 올리고사카라이드 샘플의 세파덱스(Sephadex) G-50 칼럼으로부터의 용출 패턴을 도시하고 ;
제4도는 〔3H〕티미딘을 DNA내로 혼입시킨데 대한, FGF에 대해 친화력을 갖는 올리고사카라이드 샘플의 영향을 도시한 그래프이며, 여기서, ○는 aFGF이고, ●는 aFGF+트립신 처리를, △는 aFGF+FGF=에 대해 친화력을 갖는 올리고사카라이드+트립신 처리를 ▲는 aFGF+FGF에 대해 친화력을 갖지 않는 올리고사카라이드+트립신 처리를 나타낸다.

Claims (9)

  1. 주요 디사카라이드 단위가 L-이두론산 2-설페이트 및 N-설포-D-글루코사민을 포함하는 8 내지 18개의 모노사카라이드 잔기로 이루어진, 섬유아세포 성장 인자에 대한 친화성을 갖는 올리고사카라이드.
  2. 헤파란 설페이트를 헤파리티나제 I로 분해시킴을 특징으로 하여, 주요 디사카라이드 단위가 L-이두론산 2-설페이트 및 N-설포-D-글루코사민을 포함하는 8 내지 18개의 모노사카라이드 잔기로 이루어진, 섬유아세포 성장 인자에 대한 친화성을 갖는 올리고사카라이드를 제조하는 방법.
  3. 제2항에 있어서, 생성된 분해물을 콘드로이틴설페이트의 존재하에 산성 또는 염기성 섬유아세포 성장 인자-결합 담체와 함께 배양시킨 다음, 상기 담체로부터 결합 분해물을 탈착시키는 방법.
  4. 제2항에 있어서, 헤파란 설페이트가 소, 돼지 또는 양의 소장 또는 대동맥 및 집에서 기르는 수탉의 볏으로부터 수득되는 방법.
  5. 제3항에 있어서, 상기 담체가 아가로즈 겔인 방법.
  6. 헤파란 설페이트를 산성 또는 염기성 섬유아세포 성장 인자-결합 담체와 함께 배양시키고 이 담체에 결합된 헤파란설페이트의 분획을 분리시켜서 분리된 헤파란 설페이트 분획을 헤파리티나제 I로 분해시킴을 특징으로 하여, 주요 디사카라이드 단위가 L-이두론산 2-설페이트 및 N-설포-D-글루코사민을 포함하는 8 내지 18개의 모노사카라이드 잔기로 이루어진, 섬유아세포 성장 인자에 대한 친화성을 갖는 올리고사카라이드를 제조하는 방법.
  7. 제6항에 있어서, 생성된 분해물을 콘드로이틴설페이트의 존재하에 산성 또는 염기성 섬유아세포 성장 인자-결합 담체와 함께 배양시킨 다음, 상기 담체로 부터 결합분해물을 탈착시키는 방법.
  8. 주요 디사카라이드 단위가 L-이두론산 2-설페이트 및 N-설포-D-글루코사민을 포함하는 8 내지 18개의 모노사카라이드 잔기로 이루어진, 섬유아세포 성장 인자에 대한 친화성을 갖는 올리고사카라이드를 2개 이상 포함하는 조성물.
  9. 섬유아세포 성장 인자 및 1개 이상의 주요 디사카라이드 단위가 L-이두론산 2-설페이트 및 N-설페이트 및 N-설포-D-글루코사민을 포함하는 8 내지 18개의 모노사카라이드 잔기로 이루어진 섬유아세포 성장 인자에 대한 친화성을 갖는 올리고사카라이드를 약제학적으로 허용되는 담체와 함께 포함하는 약제학적 조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019920006345A 1991-04-16 1992-04-16 섬유아세포 성장 인자에 대해 친화성을 갖는 올리고사카라이드 및 이의 제조방법 KR920019810A (ko)

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JP11090591 1991-04-16
JP91-110905 1991-04-16

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US (1) US5849722A (ko)
EP (1) EP0509517B1 (ko)
KR (1) KR920019810A (ko)
AT (1) ATE148470T1 (ko)
AU (1) AU656953B2 (ko)
CA (1) CA2066305A1 (ko)
DE (1) DE69217104T2 (ko)
FI (1) FI921689A (ko)
MX (1) MX9201801A (ko)
NO (1) NO921495L (ko)
ZA (1) ZA922830B (ko)

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US6140481A (en) * 1994-07-01 2000-10-31 Seikagaku Corporation Process for producing desulfated polysaccharide, and desulfated heparin
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JP4262979B2 (ja) * 2000-11-21 2009-05-13 ザ・テキサス・エイ・アンド・エム・ユニバーシテイ・システム Ｆｇｆアフィニティークロマトグラフィー
WO2002076475A2 (en) * 2001-03-23 2002-10-03 Bioparken As Glycosaminoglycan anticoagulants derived from fish
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US20060183712A1 (en) * 2005-02-17 2006-08-17 The Texas A&M University System Affinity purified heparin/heparan sulfate for controlling the biological activity of the FGF receptor
JP6070693B2 (ja) * 2012-03-30 2017-02-01 味の素株式会社 硫酸化化合物を含む幹細胞増殖用培地
JP6321786B2 (ja) 2013-05-16 2018-05-09 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ ヘパラン硫酸
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CN104530262B (zh) * 2015-01-26 2016-08-24 安徽科宝生物工程有限公司 一种从猪肺中提取肝素的生产方法

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EP0509517A3 (en) 1993-09-01
MX9201801A (es) 1992-10-30
ZA922830B (en) 1993-01-27
NO921495L (no) 1992-10-19
US5849722A (en) 1998-12-15
NO921495D0 (no) 1992-04-15
ATE148470T1 (de) 1997-02-15
DE69217104D1 (de) 1997-03-13
EP0509517B1 (en) 1997-01-29
AU1500392A (en) 1993-03-11
FI921689A0 (fi) 1992-04-15
EP0509517A2 (en) 1992-10-21
DE69217104T2 (de) 1997-06-05
FI921689A (fi) 1992-10-17
CA2066305A1 (en) 1992-10-17
AU656953B2 (en) 1995-02-23

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