KR920012443A - Method for preparing hysine by recombinant Corynebacterium glutamicum - Google Patents

Method for preparing hysine by recombinant Corynebacterium glutamicum Download PDF

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Publication number
KR920012443A
KR920012443A KR1019900022963A KR900022963A KR920012443A KR 920012443 A KR920012443 A KR 920012443A KR 1019900022963 A KR1019900022963 A KR 1019900022963A KR 900022963 A KR900022963 A KR 900022963A KR 920012443 A KR920012443 A KR 920012443A
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South Korea
Prior art keywords
corynebacterium glutamicum
transformed
hysine
preparing
dap
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KR1019900022963A
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Korean (ko)
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KR920008381B1 (en
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한종권
오종원
이현환
정 스테펜
현형환
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김정순
제일제당 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음No content

Description

재조함 코리네박테리움 글루타미쿰에 의한 하이신 제조방법Preparation of Hysine by Corynebacterium glutamicum

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1든 dapA 유전자 크로닝 개요도이다. 제2도는 라이신 생산균주인 코리네박테리움 글루타미쿰 CS-755에 dapA 유전자 도입을 위한 셔틀벡터 pECCG 117 및 pECCG 122와의 복합 프라스미드 제작 개요도이다.The first is a schematic diagram of dapA gene cloning. FIG. 2 is a schematic diagram of complex plasmid preparation with shuttle vectors pECCG 117 and pECCG 122 for introducing dapA gene into Corynebacterium glutamicum CS-755, a lysine producing strain.

Claims (4)

코리네박테리움 글루타미쿰 dap A 유전자를 함유한 코리네박테리움 글루타미쿰 및 대장균에서 복제되고 발현되는 발현히쿨 pDHDP 19.Expression hikul pDHDP cloned and expressed in Corynebacterium glutamicum and E. coli containing Corynebacterium glutamicum dap A gene. 코리네박테리움 글루타미쿰 dap A 유전자를 함유한 코리네박테리움 글루타미쿰 및 대장균에서 복제되고 발현되는 발현비히쿨로 형질전환된 코리네박테리움 글루타미쿰.Corynebacterium glutamicum containing the dap A gene Corynebacterium glutamicum and Corynebacterium glutamicum transformed with expression Bichkul which is cloned and expressed in E. coli. 제2항에 있어서, 형질전환된 코리네박테리움 글루타미쿰이 α-아미노에틸-β-시스테인에 내성을 가지며 L-라이신을 생산함을 특징으로 하는 형질전환된 코리네박테리움 글루타미쿰 KFCC 10720.3. The transformed Corynebacterium glutamicum KFCC according to claim 2, wherein the transformed Corynebacterium glutamicum is resistant to α-aminoethyl-β-cysteine and produces L-lysine. 10720. 코리네박테리움 글루타미쿰 dap A 유전자를 함유한 코리네박테리움 글루타미쿰 및 대장균에서 복제되고 발현되는 발현비히클 pDHDP 19로 형질전환된 코리네박테리움 글루타미쿰을 배양하여 라이신을 생산하는 방법.Method for producing lysine by culturing Corynebacterium glutamicum containing Corynebacterium glutamicum dap A gene and Corynebacterium glutamicum transformed with expression vehicle pDHDP 19 cloned and expressed in Escherichia coli . ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.※ Note: This is to be disclosed by the original application.
KR1019900022963A 1990-12-31 1990-12-31 Method for producins l-lysine KR920008381B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900022963A KR920008381B1 (en) 1990-12-31 1990-12-31 Method for producins l-lysine

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Application Number Priority Date Filing Date Title
KR1019900022963A KR920008381B1 (en) 1990-12-31 1990-12-31 Method for producins l-lysine

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KR920012443A true KR920012443A (en) 1992-07-27
KR920008381B1 KR920008381B1 (en) 1992-09-26

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10032173A1 (en) * 2000-07-01 2002-01-17 Degussa New nucleotide sequences encoding the plsC gene
EP1307477A2 (en) * 2000-08-02 2003-05-07 Degussa AG Nucleotide sequences which code for the metf gene
EP1307476A1 (en) * 2000-08-02 2003-05-07 Degussa AG Nucleotide sequences which code for the mete gene
WO2002010209A1 (en) * 2000-08-02 2002-02-07 Degussa Ag Nucleotide sequences which code for the meth gene
DE10109690A1 (en) * 2000-09-02 2002-03-14 Degussa New nucleotide sequences encoding the metY gene

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