KR920000933A - Novel shuttle vector expresses in Escherichia coli and coryneform bacteria with multiple cleavage sites - Google Patents

Novel shuttle vector expresses in Escherichia coli and coryneform bacteria with multiple cleavage sites Download PDF

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KR920000933A
KR920000933A KR1019900009199A KR900009199A KR920000933A KR 920000933 A KR920000933 A KR 920000933A KR 1019900009199 A KR1019900009199 A KR 1019900009199A KR 900009199 A KR900009199 A KR 900009199A KR 920000933 A KR920000933 A KR 920000933A
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escherichia coli
shuttle
shuttle vector
corynebacterium glutamicum
multiple cleavage
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KR1019900009199A
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KR920007401B1 (en
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김성준
오종원
노갑수
정 스테펜
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김정순
제일제당 주식회사
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium

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Abstract

내용없음No content

Description

다발성 절단부위를 지닌 대장균과 코리네형 세균에서 발현 가능한 신규 셔특벡터Novel Sherk vector capable of expression in Escherichia coli and coryneform bacteria with multiple cleavage sites

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 코리네형 세균과 대장균에서 발현되는 셔틀벡터(Shuttle vector)pECCG1으로 부터 축소된 셔틀벡터의 제작도이다.FIG. 1 is a drawing of a shuttle vector reduced from a shuttle vector pECCG1 expressed in coryneform bacteria and E. coli.

제2도는 축소된 셔틀벡터에 다발성 절단부위(Multiple cloning site)를 도입시킨 신규 셔틀벡터 pECCG122와 pECCG123의 제작도이다.2 is a schematic diagram of the novel shuttle vectors pECCG122 and pECCG123 in which multiple cloning sites are introduced into a reduced shuttle vector.

Claims (8)

코리네박테리움 글루타미쿰 및 대장균에서 복제되며 pBluescript II KS+다발성 절단부위를 지닌 셔틀벡터.Shuttle vector cloned from Corynebacterium glutamicum and Escherichia coli and having a pBluescript II KS + multiple cleavage site. 제1항에 있어서, pBluescript II KS+다발성 절단부위가, 코래네박테리움 글루타미쿰 및 대장균에서 복제되며, pACYC177을 BamHI으로 pCB1을 BCℓI으로 절단하여 T4 DNA리가제로 접합하여 만든 pECCG1에 도입됨을 특징으로 하는 셔틀벡터.The pBluescript II KS + multiple cleavage site is cloned from Corynebacterium glutamicum and Escherichia coli, and introduced into pECCG1 made by cutting pACYC177 with BamHI and pCB1 with BCLI and conjugating with T4 DNA ligase. Shuttle vector. 제1항에 있어서, 셔틀벡터가 pECCG122(KFCC10696)임을 특징으로 하는 셔틀텍터.The shuttle detector according to claim 1, wherein the shuttle vector is pECCG122 (KFCC10696). 제2항에 있어서, 셔틀벡터가 pECCG123(KFCC10697)임을 특징으로 하는 셔틀텍터.The shuttle detector according to claim 2, wherein the shuttle vector is pECCG123 (KFCC10697). 셔특벡터 pECCG 122(KFCC 10696) 및 pECCG 123(KFCC 10697)의 복제성 및 항생제 내성의 기능을 손실받지 않음을 특징으로 하는 외부유전자를 도입하여 재조합 플라스미드를 제조하는 방법.A method for preparing a recombinant plasmid by introducing an exogenous gene characterized by not losing the function of the replication and antibiotic resistance of the sherk vectors pECCG 122 (KFCC 10696) and pECCG 123 (KFCC 10697). 셔틀벡터 pECCG122 및 pECCG123로 형질전환됨을 특징으로 하는 대장균 및 코리네박테리움 글루타미쿰.Escherichia coli and Corynebacterium glutamicum characterized by transformation with shuttle vectors pECCG122 and pECCG123. 제6항에 있어서, 셔틀벡터 pECCG122 및 pECCG123의 복제성 및 항생제 내성의 기능을 손실받지 않고 외부 유전자를 도입하여 제조된 재조합 플라스미드로 형질전환됨을 특징으로 하는 대장균 및 코리네박테리움 글루타미쿰.The Escherichia coli and Corynebacterium glutamicum according to claim 6, characterized in that they are transformed with a recombinant plasmid prepared by introducing an external gene without losing the function of the replication and antibiotic resistance of the shuttle vectors pECCG122 and pECCG123. 페니실린 G 0.1-0.1 unit/ml가 첨가된 배지에서 배양한 코리네형 세균을 이용하여 원형질체 형성없이 전기장 충격법을 사용하여 DNA을 형질전환함을 특징으로 하는 방법.Penicillin G 0.1-0.1 unit / ml method using a coryneform bacteria cultured in the medium is added, characterized in that for transforming DNA by using electric field bombardment without protoplast formation. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900009199A 1990-06-21 1990-06-21 Novel shuttle vector KR920007401B1 (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011004962A2 (en) 2009-07-08 2011-01-13 씨제이제일제당 (주) Method for producing l-lysine using corynebacterium sp. that has obtained the activity of glyceraldehyde-3-phosphate dehydrogenase derived from an alien species
WO2013109121A1 (en) 2012-01-20 2013-07-25 씨제이제일제당 (주) Recombinant microorganism with improved putrescine productivity, and method for producing putrescine using same

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9005957B2 (en) 2008-10-20 2015-04-14 Cj Cheiljedang Corporation Corynebacterium genus microorganism having ability to produce N-acetyl glucosamine and method for producing N-acetyl glucosamine or glucosamine using the same
KR20140095850A (en) 2013-01-25 2014-08-04 삼성전자주식회사 Shuttle vector for Escherichia coli and Corynebacteria
CN110283823B (en) 2016-08-31 2023-05-12 Cj第一制糖株式会社 Novel promoter and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011004962A2 (en) 2009-07-08 2011-01-13 씨제이제일제당 (주) Method for producing l-lysine using corynebacterium sp. that has obtained the activity of glyceraldehyde-3-phosphate dehydrogenase derived from an alien species
WO2013109121A1 (en) 2012-01-20 2013-07-25 씨제이제일제당 (주) Recombinant microorganism with improved putrescine productivity, and method for producing putrescine using same

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