KR920002781A - Cellulase gene of Bacillus licheniformis - Google Patents

Cellulase gene of Bacillus licheniformis Download PDF

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Publication number
KR920002781A
KR920002781A KR1019900010456A KR900010456A KR920002781A KR 920002781 A KR920002781 A KR 920002781A KR 1019900010456 A KR1019900010456 A KR 1019900010456A KR 900010456 A KR900010456 A KR 900010456A KR 920002781 A KR920002781 A KR 920002781A
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South Korea
Prior art keywords
bacillus licheniformis
cellulase gene
gene
cellulase
cellulose
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KR1019900010456A
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Korean (ko)
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KR920006108B1 (en
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최진영
소성
박종경
정교민
장이섭
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김만경
태평양화학 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음.No content.

Description

바실러스 라이케니포미스(Bacillus licheniformis)의 셀룰레이스(cellulase) 유전자Cellulase gene of Bacillus licheniformis

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 콩고레드(congored) 염색방법으로 셀룰레이스(cellulase)가 클로닝된 재조합 플라스미드를 갖고 있는 형질전환주를 선별한 사진이다.FIG. 1 is a photograph of a transformant having a recombinant plasmid cloned with cellulase by a Congo red staining method.

제2도는 에스케리치아 콜라이(E. coli)에 클로닝된 셀룰레이스 유전자 인서트 크기를 최종적으로 1.4kb로 줄인 pLC110의 제한효소지도를 나타내는 그림이다. 실선으로 표시된 Hind III/EcoRI 단편이 삽입된 셀룰레이스 유전자를 나타낸다.Figure 2 shows the restriction map of pLC110, which finally reduced the size of the cellulase gene insert cloned into E. coli to 1.4 kb. The Hind III / EcoRI fragment indicated by the solid line represents the cellulase gene inserted.

제3도는 클로닝된 셀룰레이스 유전자의 DNA 염기서열을 밝히기 위해 사용한 DNA의 방향을 나타낸다.3 shows the orientation of the DNA used to reveal the DNA sequence of the cloned cellulose gene.

제4도는 결정된 DNA 염기배열과 이로부터 추정된 아미노산 배열 순서를 나타낸다.4 shows the determined DNA nucleotide sequence and the amino acid sequence sequence estimated therefrom.

제5도는 에스케리치아 콜라이 표준균주와 셀룰레이스 유전자가 클로닝된 균주의 조 추출액을 폴리아크릴아마이드겔 전기영동한 사진도해이다.FIG. 5 is a photographic diagram of polyacrylamide gel electrophoresis of crude extracts of Escherichia coli standard strains and strains cloned with cellulose genes.

Claims (2)

바실러스 라이케니포미스 KCTC 1026의 셀룰레이스를 코딩(coding)하며 계면활성제 및 단백분해효소에 대해 내성을 갖는 셀룰레이스 유전자.A cellulose gene that encodes the cellulase of Bacillus Lycheniformis KCTC 1026 and is resistant to surfactants and proteases. 제1항에 있어서, 다음과 같은 아미노산 배열을 갖는 셀룰레이스를 유전자.The gene according to claim 1, wherein the cellulose has the following amino acid sequence. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900010456A 1990-07-11 1990-07-11 Cellulase gene in bacillus licheniformis KR920006108B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900010456A KR920006108B1 (en) 1990-07-11 1990-07-11 Cellulase gene in bacillus licheniformis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900010456A KR920006108B1 (en) 1990-07-11 1990-07-11 Cellulase gene in bacillus licheniformis

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KR920002781A true KR920002781A (en) 1992-02-28
KR920006108B1 KR920006108B1 (en) 1992-07-27

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KR1019900010456A KR920006108B1 (en) 1990-07-11 1990-07-11 Cellulase gene in bacillus licheniformis

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KR920006108B1 (en) 1992-07-27

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