KR920002780A - Cellulase gene of Bacillus sp. - Google Patents

Cellulase gene of Bacillus sp. Download PDF

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Publication number
KR920002780A
KR920002780A KR1019900010421A KR900010421A KR920002780A KR 920002780 A KR920002780 A KR 920002780A KR 1019900010421 A KR1019900010421 A KR 1019900010421A KR 900010421 A KR900010421 A KR 900010421A KR 920002780 A KR920002780 A KR 920002780A
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South Korea
Prior art keywords
bacillus
cellulase gene
gene
cellulase
cellulose
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KR1019900010421A
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Korean (ko)
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KR920009506B1 (en
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최진영
소성
박종경
정교민
장이섭
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김만경
태평양화학 주식회사
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Publication of KR920002780A publication Critical patent/KR920002780A/en
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Publication of KR920009506B1 publication Critical patent/KR920009506B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Biochemistry (AREA)
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  • Enzymes And Modification Thereof (AREA)

Abstract

내용 없음No content

Description

바실러스 스페시스(Bacillus sp.)의 샐룰레이스(celluase)유전자Cellulase gene of Bacillus sp.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 콩고레드(cong red)염색방법으로 셀룰레이스(cellulase)가 클로닝된 제조합플라스미드를 갖고 있는 형질전화주를 선별한 사진이다.FIG. 1 is a photograph of a transgenic strain having a synthetic plasmid cloned with cellulase by cong red staining.

제2도는 에스케리치아 콜라이(E. coli)에 클리닝된 셀룰레이스 유전자 인서트크기를 최종적으로 1.8kb로 줄인 pBC110의 제한효소지도를 나타내는 그림이다. 실선으로 표시된 Hind Ⅲ/EcoRI 단편이 삽입된 셀룰레이스 유전자를 나타낸다.Figure 2 shows the restriction map of pBC110, which finally reduced the size of the cellulase gene insert cleaned to E. coli to 1.8 kb. The Hind III / EcoRI fragment indicated by the solid line represents the cellulase gene inserted.

제3도는 클로닝된 셀룰레이스 유전자의 염기서열을 결정하기 위해 사용한 전략을 나타내는 그리이다.3 is a diagram showing the strategy used to determine the base sequence of the cloned cellulose gene.

Claims (2)

바실러스 스페시스의 셀룰레이스 중에서 다음과 같은 DNA 염기서열을 갖는 셀룰레이스를 코딩(Coding)하는 유전자Gene coding for cellulose having the following DNA sequencing among the cellulase of Bacillus sp. 제1항에 있어서, 염기서열 산물이 계면활성제 및 단백분해 효소에 내성을 갖는 셀룰레이스 유전자.The cellulose gene of claim 1, wherein the sequencing product is resistant to surfactants and proteases. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900010421A 1990-07-10 1990-07-10 Gene for cellulase of bacillus sp KR920009506B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900010421A KR920009506B1 (en) 1990-07-10 1990-07-10 Gene for cellulase of bacillus sp

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900010421A KR920009506B1 (en) 1990-07-10 1990-07-10 Gene for cellulase of bacillus sp

Publications (2)

Publication Number Publication Date
KR920002780A true KR920002780A (en) 1992-02-28
KR920009506B1 KR920009506B1 (en) 1992-10-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900010421A KR920009506B1 (en) 1990-07-10 1990-07-10 Gene for cellulase of bacillus sp

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Publication number Publication date
KR920009506B1 (en) 1992-10-17

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