KR910006492A - Method for preparing poly-beta-hydroxy butyric acid - Google Patents

Method for preparing poly-beta-hydroxy butyric acid Download PDF

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Publication number
KR910006492A
KR910006492A KR1019890012683A KR890012683A KR910006492A KR 910006492 A KR910006492 A KR 910006492A KR 1019890012683 A KR1019890012683 A KR 1019890012683A KR 890012683 A KR890012683 A KR 890012683A KR 910006492 A KR910006492 A KR 910006492A
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South Korea
Prior art keywords
culture
concentration
phb
nitrogen source
source
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KR1019890012683A
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Korean (ko)
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KR910004370B1 (en
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임건빈
김갑진
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이상운
고려합섬 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음.No content.

Description

폴리-베타-하이드록시 부틸산(poly-β-hydroxy butyric acid)의 제조방법Method for preparing poly-beta-hydroxy butyric acid

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

Claims (5)

PHB를 생산하기 위한 배양공정에 있어서 알칼리지너스 유트로퍼스 H-16(Alcaligenes eutrophus H-16)이나 이들의 변이주를 이용하여 배양온도 : 30℃-34℃, pH 6.7-7.2, 용존산소농도 : 4-6PPM 조건에서 배양하면서 탄소원과 질소원, 인(phosphorus)및 그밖의 미량원소들을 포함한 수용액 상태의 영양배지를 배양액중에 대수적(Exponential)으로 공급함으로써 균증식속도를 일정하게 유지하여 균체농도를 극대화한 후, 질소원은 낮은 농도로 계속 유지하면서 탄수원은 배양액중에 일정하게 공급하고 배양조 내부에 약한 압력을 가하여 용존산소 농도를 0.5-1.5PPM으로 유지시켜 줌으로써 균체내에 고농도의 PHB를 축적시키는 PHB 제조방법.In the culturing process for producing PHB, Alkali Genus Eutrophus H-16 or its mutants were cultured at 30 ℃ -34 ℃, pH 6.7-7.2, dissolved oxygen concentration: 4 While cultivating at -6PPM conditions, nutrient medium in aqueous solution containing carbon source, nitrogen source, phosphorus and other trace elements is supplied algebraically in the culture solution to maximize the cell concentration by maintaining constant growth rate. , PHB manufacturing method to accumulate high concentration of PHB in the cells by maintaining a nitrogen source at a constant concentration, while supplying carbohydrates in the culture medium and applying a weak pressure in the culture tank to maintain a dissolved oxygen concentration of 0.5-1.5PPM. 제 1 항에 있어서, 알칼리지너스 유트퍼스 H-16(ATTC-17699, KCTC-1006)이나 이들의 변이주를 사용하여 과당시럽(F-55, F-42)을 탄소원으로 이용하는 PHB 제조방법.The method for producing PHB according to claim 1, wherein fructose syrup (F-55, F-42) is used as a carbon source by using alkaline genus Euphorus H-16 (ATTC-17699, KCTC-1006) or a mutant thereof. 제 1 항에 있어서, 암모니아수와 옥수수전분 당화액(C.S.L)을 질소원과 에너지원으로 이용하는 PHB 제조방법.The method of claim 1, wherein ammonia water and corn starch saccharification solution (C.S.L) are used as a nitrogen source and an energy source. 제 1 항에 있어서, 탄소원/질소원의 질량비를 균체 증식속도에 맞춰 배양초기에 6-15 : 1W/W로 공급하고 배양말기에는 15-40 : 1W/W로 공급하는 PHB 제조방법.The method of claim 1, wherein the mass ratio of the carbon source / nitrogen source is supplied at 6-15: 1W / W at the beginning of the culture and 15-40: 1W / W at the end of the culture. 제 1 항에 있어서, 배양온도를 33℃로 하고 pH를 6.7-6.8로 유지하며 발효조내에 0.2-1.0kgf/cm의 압력을 가해주는 고농도 PHB 제조방법.The method of claim 1, wherein the culture temperature is 33 ° C., the pH is maintained at 6.7-6.8, and the concentration of 0.2-1.0 kgf / cm is applied to the fermenter. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019890012683A 1989-09-01 1989-09-01 Method for preparation of poly-beta-hydroxy butyric acid KR910004370B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019890012683A KR910004370B1 (en) 1989-09-01 1989-09-01 Method for preparation of poly-beta-hydroxy butyric acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019890012683A KR910004370B1 (en) 1989-09-01 1989-09-01 Method for preparation of poly-beta-hydroxy butyric acid

Publications (2)

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KR910006492A true KR910006492A (en) 1991-04-29
KR910004370B1 KR910004370B1 (en) 1991-06-26

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KR1019890012683A KR910004370B1 (en) 1989-09-01 1989-09-01 Method for preparation of poly-beta-hydroxy butyric acid

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KR910004370B1 (en) 1991-06-26

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