KR950003448A - Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation - Google Patents
Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation Download PDFInfo
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- KR950003448A KR950003448A KR1019930012673A KR930012673A KR950003448A KR 950003448 A KR950003448 A KR 950003448A KR 1019930012673 A KR1019930012673 A KR 1019930012673A KR 930012673 A KR930012673 A KR 930012673A KR 950003448 A KR950003448 A KR 950003448A
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- South Korea
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- phb
- cell
- nitrogen source
- concentration
- butyric acid
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 미생물 발효법에 의한 폴리베타하이드록시 부티르산(PHB)의 제조방법에 관한 것으로, 생분해성 플라스틱의 일종인 PHB를 알칼리제니스속의 균주를 이용하여 농축영양배지에서 배양온도 30~35℃, pH 6.5~7.0의 조건하에서 전체 성장속도에 따라서 탄소원을 대수적으로 공급하여 호기적으로 배양하고 배양에 필요한 질소원과 pH조절용 알칼리 수용액으로 암모니수를 공급함으로써 균체 농도를 100~200g/l까지 증식시킨 후, 질소원, 인, 마그네숨, 황 등과 용존산소의 고갈을 유도하여 균체세포내의 PHB의 함유율을 79~90%까지 증가시킬 수 있다.The present invention relates to a method for producing polybetahydroxy butyric acid (PHB) by the microbial fermentation method, PHB, a kind of biodegradable plastic, using a strain of alkali genus in a culture medium 30 ~ 35 ℃, pH 6.5 Under a condition of ˜7.0, the carbon source was supplied algebraically in accordance with the overall growth rate, followed by culturing aerobically, and by supplying the ammonia water with the nitrogen source needed for the cultivation and the alkaline aqueous solution for pH adjustment, the cell concentration was increased to 100-200 g / l, and then the nitrogen source. By inducing depletion of dissolved oxygen, phosphorus, magnesium, sulfur and the like, the content of PHB in cell cells can be increased by 79-90%.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019930012673A KR950003448A (en) | 1993-07-07 | 1993-07-07 | Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019930012673A KR950003448A (en) | 1993-07-07 | 1993-07-07 | Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation |
Publications (1)
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KR950003448A true KR950003448A (en) | 1995-02-16 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019930012673A KR950003448A (en) | 1993-07-07 | 1993-07-07 | Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100677952B1 (en) * | 2004-08-13 | 2007-02-05 | 한국생명공학연구원 | A fermentation strategy for producing recombinant protein in host cells |
-
1993
- 1993-07-07 KR KR1019930012673A patent/KR950003448A/en not_active Application Discontinuation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100677952B1 (en) * | 2004-08-13 | 2007-02-05 | 한국생명공학연구원 | A fermentation strategy for producing recombinant protein in host cells |
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