KR950003448A - Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation - Google Patents

Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation Download PDF

Info

Publication number
KR950003448A
KR950003448A KR1019930012673A KR930012673A KR950003448A KR 950003448 A KR950003448 A KR 950003448A KR 1019930012673 A KR1019930012673 A KR 1019930012673A KR 930012673 A KR930012673 A KR 930012673A KR 950003448 A KR950003448 A KR 950003448A
Authority
KR
South Korea
Prior art keywords
phb
cell
nitrogen source
concentration
butyric acid
Prior art date
Application number
KR1019930012673A
Other languages
Korean (ko)
Inventor
임건빈
김갑진
이승근
Original Assignee
이상운
고려합섬 주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 이상운, 고려합섬 주식회사 filed Critical 이상운
Priority to KR1019930012673A priority Critical patent/KR950003448A/en
Publication of KR950003448A publication Critical patent/KR950003448A/en

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 미생물 발효법에 의한 폴리베타하이드록시 부티르산(PHB)의 제조방법에 관한 것으로, 생분해성 플라스틱의 일종인 PHB를 알칼리제니스속의 균주를 이용하여 농축영양배지에서 배양온도 30~35℃, pH 6.5~7.0의 조건하에서 전체 성장속도에 따라서 탄소원을 대수적으로 공급하여 호기적으로 배양하고 배양에 필요한 질소원과 pH조절용 알칼리 수용액으로 암모니수를 공급함으로써 균체 농도를 100~200g/l까지 증식시킨 후, 질소원, 인, 마그네숨, 황 등과 용존산소의 고갈을 유도하여 균체세포내의 PHB의 함유율을 79~90%까지 증가시킬 수 있다.The present invention relates to a method for producing polybetahydroxy butyric acid (PHB) by the microbial fermentation method, PHB, a kind of biodegradable plastic, using a strain of alkali genus in a culture medium 30 ~ 35 ℃, pH 6.5 Under a condition of ˜7.0, the carbon source was supplied algebraically in accordance with the overall growth rate, followed by culturing aerobically, and by supplying the ammonia water with the nitrogen source needed for the cultivation and the alkaline aqueous solution for pH adjustment, the cell concentration was increased to 100-200 g / l, and then the nitrogen source. By inducing depletion of dissolved oxygen, phosphorus, magnesium, sulfur and the like, the content of PHB in cell cells can be increased by 79-90%.

Description

미생물 발효법에 의한 폴리-베타-히드록시 부티르산(Poly-β-Hydroxy Butyric Acid : PHB)의 제조방법Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (4)

생분해성 폴리베타하이드록시 부티르산(Poly-β-Hydroxy Butyric Acid 일명 PHB)를 미생물 발효법으로 생산하는 공정에 있어서, 알칼리제니스(Alcaligenes)속의 균주를 이용하여 발효전체 공정에 요구되는 영양원소들을 초기에 충분히 함유하는 농축 영양 배지에서 배양온도 30~35℃, pH 6.5~7.0의 조건하에서 균체성장 정도에 따라서 탄소원을 대수적으로 공급하여 호기적으로 배양을 수행하고, 배양에 필요한 질소원과 pH조절용 알칼리 수용액으로서 10~30%의 암모니아수를 공급함으로써 균체농도를 100~200g/l까지 증식시킨 후, 질소원, 인, 마그네슘, 황 등과 용존산소와 고갈을 유도하여 균체 세포내에 PHB의 함유율을 70~90%까지 증가시킴을 특징으로 하는 PHB의 제조방법.In the process of producing biodegradable polybeta-hydroxybutyric acid (Poly-β-Hydroxy Butyric Acid aka PHB) by microbial fermentation method, the strains of Alcaligenes genus are used to sufficiently satisfy the nutrients required for the whole fermentation process. In a concentrated nutrient medium containing a carbon source in a logarithmic growth according to the degree of cell growth under a culture temperature of 30 ~ 35 ℃, pH 6.5 ~ 7.0 to perform aerobic cultivation, 10 as a nitrogen source and an alkaline aqueous solution for pH control Increase the cell concentration to 100 ~ 200g / l by supplying ~ 30% ammonia water, and induce the depletion of dissolved oxygen and nitrogen source, phosphorus, magnesium, sulfur and so on, and increase the content of PHB in the cell cells by 70 ~ 90% PHB manufacturing method characterized in that. 제1항에 있어서 균체성장 속도는 가스 분석기를 이용하여 발효배기가스 중의 CO2발생농도(CER)를 모니터링하여 탄소원의 공급속도를 결정하는 PHB의 제조방법.The method of claim 1, wherein the cell growth rate is determined by monitoring the CO 2 generation concentration (CER) in the fermentation exhaust gas using a gas analyzer to determine the supply rate of the carbon source. 제1항에 있어서 공급되는 영양배지 중의 탄소원과 질소원의 비율(C/N ratio)조절없이 pH조절을 위해 공급되는 암모니아수의 농도만으로써 배양액중의 질소원 농도를 변화시켜 균체성장과 PHB축적조건을 조절함을 특징으로 하는 PHB의 제조방법.According to claim 1, by controlling the cell growth and PHB accumulation conditions by changing the concentration of nitrogen source in the culture medium only by the concentration of ammonia water supplied for pH control without adjusting the ratio of carbon source and nitrogen source (C / N ratio) in the nutrient medium supplied. PHB manufacturing method characterized in that. 제1항에 있어서 PHB축적전 균체농도가 100~300g/l이며, 배양 종료시에 PHB축적율이 건조균체량의 80~90%임을 특징으로 하는 PHB의 제조방법.The method for producing PHB according to claim 1, wherein the cell concentration before PHB accumulation is 100-300 g / l, and the PHB accumulation rate is 80-90% of the dry cell weight at the end of the culture. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930012673A 1993-07-07 1993-07-07 Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation KR950003448A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019930012673A KR950003448A (en) 1993-07-07 1993-07-07 Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930012673A KR950003448A (en) 1993-07-07 1993-07-07 Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation

Publications (1)

Publication Number Publication Date
KR950003448A true KR950003448A (en) 1995-02-16

Family

ID=67142780

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019930012673A KR950003448A (en) 1993-07-07 1993-07-07 Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation

Country Status (1)

Country Link
KR (1) KR950003448A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100677952B1 (en) * 2004-08-13 2007-02-05 한국생명공학연구원 A fermentation strategy for producing recombinant protein in host cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100677952B1 (en) * 2004-08-13 2007-02-05 한국생명공학연구원 A fermentation strategy for producing recombinant protein in host cells

Similar Documents

Publication Publication Date Title
SU1303035A3 (en) Method for fermentation of alcaligenes entrophus microorganisms for producing cells containing poly-beta-oxybutyric acid
Yahiro et al. Efficient itaconic acid production from raw corn starch
US4532210A (en) Process for producing hydrogen by alga in alternating light/dark cycle and environmental aerobic/microaerobic conditions
US5164309A (en) Process for the microbiological preparation of 1,3-propane-diol from glycerol by citrobacter
IE852782L (en) Microbiological or enzymic reactor
KR950703653A (en) Microorganisms for Tryptophan Production
CY1106177T1 (en) METHODS OF INCREASING ETHANOL PRODUCTION FROM MICROBIAL FERMENTATION
DE59812723D1 (en) PROCESS FOR THE PRODUCTION OF BIOMASS BY PHOTOSYNTHESIS
KR850004268A (en) Method for preparing poly-D-(-)-3-hydroxybutyric acid
KR850004267A (en) Method for preparing poly-D-(-)-3-hydroxybutyric acid
US4062727A (en) Process for manufacturing high density cell cultures
ZA888962B (en) Process for producing a microbial polysaccharide
KR950003448A (en) Method for preparing poly-beta-hydroxy butyric acid (PHB) by microbial fermentation
IE41489L (en) Production of glucose isomerase
GB1444072A (en) Microbiological production of proteinaceous material
GB1270006A (en) Improvements in or relating to the fermentation of methane utilizing microorganisms
JPS5743685A (en) L-glutamic oxidase
SE7712016L (en) PROCEDURE FOR THE PRODUCTION OF COENZYM Q
GB1366711A (en) Microbiological process
KR850001802A (en) How to prepare L-amino acids in bacteria
US4048013A (en) Process for producing single-cell protein from methanol using methylomonas sp. DSM 580
GB1514485A (en) Regulation of assimilable nitrogen in microbiological processes
GB1423642A (en) Process for culturing methanol-utilizing yeast
GB1451020A (en) Process for producing bacterial cells
JPS6423894A (en) Production of adipic acid

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E601 Decision to refuse application