KR910004799A - 고정상 생촉매를 사용해 효소반응에 의한 전환반응을 연속적으로 실시하는 방법 - Google Patents

고정상 생촉매를 사용해 효소반응에 의한 전환반응을 연속적으로 실시하는 방법 Download PDF

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KR910004799A
KR910004799A KR1019900010885A KR900010885A KR910004799A KR 910004799 A KR910004799 A KR 910004799A KR 1019900010885 A KR1019900010885 A KR 1019900010885A KR 900010885 A KR900010885 A KR 900010885A KR 910004799 A KR910004799 A KR 910004799A
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reaction
fixed
biocatalyst
activity
phase
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KR1019900010885A
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울리히 게이에르 한스
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원본미기재
밀레스 칼리-케미 게엠베하 앤드 캄파니 코만디트 게젤샤프트
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Publication of KR910004799A publication Critical patent/KR910004799A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/18—Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/06—Tubular

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음.

Description

고정상 생촉매를 사용해 효소반응에 의한 전환반응을 연속적으로 실시하는 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 반응기의 운전동안 촉매활성의 변화,
제2도는 결정한 촉매량과 반응기 생산성.

Claims (8)

  1. 튜브반응기 안에서 반응물질-생성물질의 전환반응에 대한 효소활성을 갖는 고정상 생촉매로 반응물질용액을 전환시켜 효소반응으로 제조할 수 있는 생성물용액을 연속적으로 제조할 수 있는 방법으로, 튜브반응기를 따라서 흐르는 반응용액에다 고정상 생촉매를 같이 흘리면서 흔들고 일정한 시간 간격으로 일정한 촉매활성분포를 반응기내에 잘 유지킴을 특징으로 하는 방법.
  2. 제1항에 있어서, 일정하게 불활성화된 고정상 생촉매를 빼주고 새활성촉매를 넣어주어 그 활성분포를 잘 유지시켜 주는 방법.
  3. 제1항에 있어서, 고정상 생촉매를 간헐적으로 넣어주고 빼주어 그 활성을 유지시키는 방법.
  4. 전항의 어느 한항에 있어서, 고정상 생촉매를 반응용액과 함께 튜브반응기 속을 통과시키며 흔드는 방법.
  5. 전항의 어느 한항에 있어서, 고정상 생촉매를 수직으로 배열한,튜브반응기, 즉 비중을 이용한 반응칼럼을 통해 흘리며 흔드는 방법.
  6. 제5항에 있어서, 첫단계에서 촉매활성의 전환율이 결정되고 잘알려진 방법으로 반응칼럼안에서 고정상 생촉매의 전환반응을 시작하고 곧이어 미리 결정한 효소활성은 초기활성의 한계치안에 두고, 연속으로 또는 일정한 시간 간격으로 새 고정상 생촉매를 원하는 높이 또는 최대한도의 높이에 도달할 때까지 주입시키는 방법.
  7. 전항의 어느 한항에 있어서, 글루코즈를 이성화하는 활성을 갖는 고정상 생촉매에다 글루코즈를 함유한 반응용액을 번환반응시켜서 글루코즈와 후락토스를 함유하는 용액을 효소반응으로 제조할 수 있는 방법.
  8. 제7항에 있어서 표시된 대로 해서 다공성 시리카나 다공성 아루미나에다 담지시킨 글루코즈 이성화효소를 고정상 생촉매로 사용하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900010885A 1989-08-11 1990-07-18 고정상 생촉매를 사용해 효소반응에 의한 전환반응을 연속적으로 실시하는 방법 KR910004799A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3926609A DE3926609A1 (de) 1989-08-11 1989-08-11 Neues verfahren zur kontinuierlichen durchfuehrung enzymatischer umsetzungen mit immobilisierten biokatalysatoren
DEP3926609.5 1989-08-11

Publications (1)

Publication Number Publication Date
KR910004799A true KR910004799A (ko) 1991-03-29

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ID=6386971

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900010885A KR910004799A (ko) 1989-08-11 1990-07-18 고정상 생촉매를 사용해 효소반응에 의한 전환반응을 연속적으로 실시하는 방법

Country Status (6)

Country Link
EP (1) EP0412467A1 (ko)
JP (1) JPH0387189A (ko)
KR (1) KR910004799A (ko)
DD (1) DD297453A5 (ko)
DE (1) DE3926609A1 (ko)
FI (1) FI903957A0 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1840203A1 (en) 2006-03-31 2007-10-03 Cargill, Inc. Enzymatic modification in a continuously regenerated packed bed column

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4158609A (en) * 1975-09-23 1979-06-19 Mueller Hans Process for the continuous conversion of products by enzyme action
IL51672A0 (en) * 1976-04-05 1977-05-31 Miles Lab Process and apparatus for carrying out enzymatic reactions in serially connected reactors
NL7709179A (nl) * 1977-08-19 1979-02-21 Stamicarbon Werkwijze voor het uitvoeren van enzymatische omzettingen.
EP0042306A3 (en) * 1980-06-17 1983-02-09 Exxon Research And Engineering Company A low energy continuous process for increasing the oxidative state of an oxidisable organic substrate
US4442216A (en) * 1982-09-29 1984-04-10 Harvey Douglas G Continuous enzymatic reactor for use of immobilized enzyme beads
DE3346861A1 (de) * 1983-12-23 1985-06-27 Krauss-Maffei AG, 8000 München Vorrichtung zum beschleunigen der stoffumsetzung zwischen zwei in einem fliessbett reagierenden medien

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EP0412467A1 (de) 1991-02-13
JPH0387189A (ja) 1991-04-11
DE3926609A1 (de) 1991-02-14
FI903957A0 (fi) 1990-08-10
DD297453A5 (de) 1992-01-09

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