KR900700622A - 미생물을 수집하고 검출하기 위한 방법 및 장치 - Google Patents

미생물을 수집하고 검출하기 위한 방법 및 장치

Info

Publication number
KR900700622A
KR900700622A KR1019890702151A KR890702151A KR900700622A KR 900700622 A KR900700622 A KR 900700622A KR 1019890702151 A KR1019890702151 A KR 1019890702151A KR 890702151 A KR890702151 A KR 890702151A KR 900700622 A KR900700622 A KR 900700622A
Authority
KR
South Korea
Prior art keywords
microorganisms
fluid
adsorbent particles
particles
openings
Prior art date
Application number
KR1019890702151A
Other languages
English (en)
Inventor
캘빈 에버솔 리차드
토마스 젤로미니 프랭크
임메트 태트낼 로버트
Original Assignee
제임스 제이. 플라인
이. 아이. 듀퐁 드 네모아 앤드 캄파니
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 제임스 제이. 플라인, 이. 아이. 듀퐁 드 네모아 앤드 캄파니 filed Critical 제임스 제이. 플라인
Publication of KR900700622A publication Critical patent/KR900700622A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

내용 없음

Description

미생물을 수집하고 검출하기 위한 방법 및 장치
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
샘플 회수 장치를 사용함을 포함한 본 발명의 방법은 제1도에 나타나 있다.

Claims (6)

  1. a) 미생물 세포를 흡착하거나 끌어들이는 흡착제 입자와 유체 샘플을 혼합하고, b) 혼합물을 진탕시켜 유체중의 흡착제 입자, 미생물 및 다른 고체 불순물의 실질적으로 균일한 현탁액을 만들고, c) 상기 현탁액을, 걸렸거나 흡착된 미생물을 함유하는 흡착제 입자만을 케익으로서 실질적으로 보유하는 필터에 통과시키고, d) 임의로는, 실질적으로 모든 가용성 불순물의 상기 케익을 세척하고, e) 미생물이 함유된 상기 흡착제 입자를 미생물에 대해 실질적으로 불활성인 순수 액체에 재현탁시켜 흡착제 입자를 미생물로부터 분리하고, f) 상기 흡착제 입자가 침전되도록 하고 이에 따라 상등액체에 미생물이 분비되도록 하는 단계를 포함함을 특징으로 하여, 분리가 어려운 여러가지 불순물이 함유된 유체 샘플로부터 미생물을 분리하고 검출하는 방법.
  2. a) 미생물 세포를 흡착하거나 끌어들이는 흡착제 입자와 유체 샘플을 혼합하고, b) 혼합물을 진탕시켜 유체중의 흡착제 입체, 미생물 및 다른 고체 불순물의 실질적으로 균일한 현탁액을 만들고, c) 상기 현탁액을, 걸렸거나 흡착된 미생물을 함유하는 흡착제 입자만을 케익으로서 실질적으로 보유하고 케익과 같은 필터에 통과시키고 d) 임의로는, 실질적으로 모든 가용성 불순물의 상이 케익을 세척하고, e) 미생물이 함유된 상기 흡착제 입자를 미생물에 실질적으로 불활성인 순수 액체에 재현탁시키고, f) 미생물 세포를 파괴하여 검출가능한 세포 성분을 분비시키고, g) 상기 미생물이 측정대상으로서 상기 성분을 검출 하는 단계를 특징으로 하여, 분리가 어려운 여러가지 불순물을 함유하는 유체 샘플로부터 미생물을 분리하고 검출하는 방법.
  3. 제2항에 있어서, 흡착제 입자가 대부분의 직경이 2내지 10 마이크론인 규조토, 입자, 펄라이트 입자 및 지올라이트 점토 입자로 이루어진 그룹으로부터 선택되는 방법.
  4. 제2항에 있어서, 단계f)의 파괴가 음파파쇄에 의해 수행되며 미생물을 면역분석 방법을 사용하여 검출하는 방법.
  5. 제2항에 있어서, 단계 c)의 현탁액이 기공크기가 약 20마이크론인 다공성 플러그를 통해 여과되는 방법.
  6. 제3항에 있어서, 유체가 유정 샘플이고 미생물이 설페이트-환원 박테리아인 방법.
    단일 개공이 있는 샘플 용기 및 한쪽 말단은 좁은 개공이 있고 다른쪽 말단은 비교적 넓은 개공(이는 샘플 용기의 개공과 공기가 새지 않게 결합되도록 개작되어 있다)이 있으며 두 개공사이의 영역이 플러그 또는 필터 물질을 함유하도록 개작된, 속이 빈 튜브로 이루어진 수집 캡을 포함함을 특징으로하는, 분리가 어려운 여러 불순물을 함유하는 유체로부터 미생물을 분리하기 위한 장치.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890702151A 1988-03-21 1989-03-20 미생물을 수집하고 검출하기 위한 방법 및 장치 KR900700622A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16971888A 1988-03-21 1988-03-21
US?07/169,718? 1988-03-21
PCT/US1989/001069 WO1989009279A1 (en) 1988-03-21 1989-03-20 Method and apparatus for collecting and detecting microorganisms

Publications (1)

Publication Number Publication Date
KR900700622A true KR900700622A (ko) 1990-08-16

Family

ID=22616903

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890702151A KR900700622A (ko) 1988-03-21 1989-03-20 미생물을 수집하고 검출하기 위한 방법 및 장치

Country Status (12)

Country Link
US (1) US5369011A (ko)
EP (1) EP0406306B1 (ko)
JP (1) JPH03503361A (ko)
KR (1) KR900700622A (ko)
CN (1) CN1037736A (ko)
AU (1) AU628328B2 (ko)
CA (1) CA1339250C (ko)
DE (1) DE68915439D1 (ko)
DK (1) DK226990A (ko)
MY (1) MY104991A (ko)
NZ (1) NZ228374A (ko)
WO (1) WO1989009279A1 (ko)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6004766A (en) * 1987-07-28 1999-12-21 Biotechnology Australia Pty Limited Method for detecting low levels of microorganisms
US5240606A (en) * 1990-07-09 1993-08-31 Cytyc Corporation Apparatus for preparing cells for examination
US5269918A (en) * 1990-07-09 1993-12-14 Cytyc Corporation Clinical cartridge apparatus
US5282978A (en) * 1990-07-09 1994-02-01 Cytyc Corporation Specimen processor method and apparatus
US5143627A (en) * 1990-07-09 1992-09-01 Cytyc Corporation Method and apparatus for preparing cells for examination
FR2791362B1 (fr) * 1999-03-24 2001-05-18 Elf Exploration Prod Procede de detection des bacteries sulfato-reductrices
US6361963B1 (en) 2000-08-02 2002-03-26 Hercules Incorporated Biofilm growth device
FR2848224B1 (fr) * 2002-12-05 2006-12-08 Indicia Biotechnology Procede de detection de microorganismes a l'aide d'un dispositif de filtration par flux tangentiel
JP4580383B2 (ja) * 2003-03-10 2010-11-10 ザ・ジョンズ・ホプキンス・ユニバーシティー 環境モニタリングおよび生物資源調査を行うための方法および装置
FR2902799B1 (fr) * 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8158411B2 (en) 2006-08-21 2012-04-17 Samsung Electronics Co., Ltd. Method of separating microorganism using nonplanar solid substrate and device for separating microorganism using the same
US20090053806A1 (en) * 2007-08-22 2009-02-26 Deirdre Daniels Coating solution and method for capturing and preserving biological materials
KR20110091719A (ko) * 2008-10-31 2011-08-12 바이오메리욱스, 인코포레이티드. 식별제를 사용한 미생물의 분리 및 특성규명 방법
JP5833450B2 (ja) 2008-12-31 2015-12-16 スリーエム イノベイティブ プロパティズ カンパニー 微小粒子を用いた生きた生物負荷の検出法
CN102325598A (zh) * 2008-12-31 2012-01-18 3M创新有限公司 用于处理样品的方法、套件和系统
BRPI0918693A2 (pt) * 2008-12-31 2016-07-26 3M Innovative Properties Co dispositivos para amostragem e métodos para concentração de microorganismos
WO2011082309A1 (en) 2009-12-30 2011-07-07 3M Innovative Properties Company Live bioload detection using microparticles
US8338182B2 (en) 2010-02-08 2012-12-25 Arizona Board of Regents, a body corporate acting for and on behalf of Arizona State University Methods and systems for fluid examination and remediation
WO2011140270A2 (en) 2010-05-05 2011-11-10 Arizona Board Of Regents For And On Behalf Of Arizona State University Methods and systems for ultra-trace analysis of liquids
EP3271067B1 (en) 2015-03-19 2020-10-21 3M Innovative Properties Company Guanidine-functionalized perlite particles, articles containing the particles, and methods of using the particles and articles
US10295537B2 (en) * 2017-02-06 2019-05-21 The United States of America as represened by the Secretary of the Air Force Biorecognition elements for rapid detection of biocontamination
GB201703383D0 (en) 2017-03-02 2017-04-19 Gargle Tech Ltd Testing for particulates
BE1025089B1 (fr) * 2017-03-30 2018-10-29 Univercells Sa Clarification de culture cellulaire
IL281102B2 (en) 2018-09-05 2024-04-01 Hero Scient Ltd Test for particle detection
US11754547B2 (en) 2020-05-19 2023-09-12 United States Of America As Represented By The Secretary Of The Air Force Biorecognition elements for detection of fungi and bacteria in fuel systems
EP4165385B1 (en) 2021-01-06 2024-04-17 Hero Scientific Ltd Filtration sampling devices
EP4416283A1 (en) * 2021-10-11 2024-08-21 Neogen Corporation Sample preparation and detection methods using enzymatic digestion

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3899394A (en) * 1972-12-26 1975-08-12 Bristol Myers Co Production of antibacterial agents
JPS50160490A (ko) * 1974-06-19 1975-12-25
JPS51142860A (en) * 1975-04-30 1976-12-08 Dowa Mining Co Ltd Method for oxidation treatment of fe2+ in waste liquor
US4116828A (en) * 1975-11-28 1978-09-26 International Telephone And Telegraph Corp. Water purification methods
SE452336B (sv) * 1978-06-16 1987-11-23 Boehringer Mannheim Gmbh Forfarande for pavisning av sjukdomsalstrande mikroorganismer vilka adsorberats extrakorporalt pa en selektivt bindande adsorbent
SE430900B (sv) * 1981-06-04 1983-12-19 Bo Gustav Mattiasson Forfarande for bestemning av biokemiska data for mikroorganismer eller encelliga organismer
EP0089983B1 (en) * 1981-10-07 1986-01-29 Commonwealth Scientific And Industrial Research Organisation Method for harvesting algae
FR2520631A1 (fr) * 1982-02-01 1983-08-05 Gozal David Procede pour floculer des particules microscopiques en suspension dans un liquide et application a la collecte des microalgues phytoplanctoniques et du zooplancton en suspension dans l'eau
US4503149A (en) * 1983-06-06 1985-03-05 Conoco Inc. Method of microbial assay
US4765892A (en) * 1984-08-29 1988-08-23 Applied Industrial Materials Corporation Sand filter media and an improved method of purifying water
US4775635A (en) * 1985-04-12 1988-10-04 E. I. Du Pont De Nemours And Company Rapid assay processor
US4753775A (en) * 1985-04-12 1988-06-28 E. I. Du Pont De Nemours And Company Rapid assay processor
EP0228460A1 (en) * 1985-07-10 1987-07-15 CLYDE, Robert A. Process and apparatus for enhancing biological and chemical reactions from high area inorganic base silica on fibers
GB2189317B (en) * 1986-04-16 1990-10-24 London Polytech Method of detecting and enumerating sulphate-reducing bacteria
GB8617646D0 (en) * 1986-07-18 1986-08-28 Manchester Inst Science Tech Recovery of biological material
US4999286A (en) * 1986-12-23 1991-03-12 E. I. Du Pont De Nemours And Company Sulfate reducing bacteria determination and control
CA1325980C (en) * 1987-04-22 1994-01-11 Sho Kikyotani Apparatus for the treatment of biological samples and treatment methods using the same

Also Published As

Publication number Publication date
AU3358089A (en) 1989-10-16
DK226990D0 (da) 1990-09-20
MY104991A (en) 1994-07-30
CN1037736A (zh) 1989-12-06
DE68915439D1 (de) 1994-06-23
WO1989009279A1 (en) 1989-10-05
NZ228374A (en) 1990-12-21
EP0406306B1 (en) 1994-05-18
EP0406306A1 (en) 1991-01-09
CA1339250C (en) 1997-08-12
DK226990A (da) 1990-09-20
AU628328B2 (en) 1992-09-17
JPH03503361A (ja) 1991-08-01
US5369011A (en) 1994-11-29

Similar Documents

Publication Publication Date Title
KR900700622A (ko) 미생물을 수집하고 검출하기 위한 방법 및 장치
Enell et al. A column method for determination of leaching of polycyclic aromatic hydrocarbons from aged contaminated soil
Al-Degs et al. Sorption behavior of cationic and anionic dyes from aqueous solution on different types of activated carbons
US3682315A (en) Cartridge type column for treatment of liquid streams and substrate enclosure therefor
Kot et al. Passive sampling for long-term monitoring of organic pollutants in water
Peltonen et al. Air sampling and analysis of polycyclic aromatic hydrocarbons
Hagen et al. Membrane approach to solid-phase extractions
US11555766B2 (en) Ultrasound-assisted solvent extraction of analytes from porous membrane packed solid samples
JP6516092B2 (ja) 残留農薬の分析用試料の調製方法
Potgieter Adsorption of methylene blue on activated carbon: an experiment illustrating both the Langmuir and Freundlich isotherms
Ling et al. Quantifying adsorption of an indicator bacteria in a soil–water system
JPH06506150A (ja) 液体試料を分離するための装置および方法
JP2018036258A (ja) 残留農薬の分析用試料の調製方法
KR870008601A (ko) 여과 장치
ATE213427T1 (de) Adsorption bzw. desorption gelöster bestandteile in flüssigkeiten und vorrichtung dafür
US4303529A (en) Multi-chromatographic materials
US4267056A (en) Sample preparation apparatus and process for the use thereof
Lane et al. Gas/particle partition measurements of PAH at Hazelrigg, UK
US4636227A (en) Monitoring by adsorption
JP2006349356A (ja) 分離処理用の充填材の製造方法
Frauenstein et al. SandTraps are efficient, scalable, and mild systems for harvesting, washing and concentrating cells
Ozcan et al. Chromatographic separation and analytic procedure for priority organic pollutants in urban air
JP2007167815A (ja) 固相抽出カートリッジおよび固相抽出方法
GB1293591A (en) Shut off filtering and sorbtion device
US20060086665A1 (en) Method for the accelerated dialytic recovery of foreign substances from passive collectors consisting of semipermeable membranes having different dimensions

Legal Events

Date Code Title Description
WITN Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid