KR900008034A - Variants of Streptomyces KCCB_2 and Preparation of Antibiotics Using the Same - Google Patents

Variants of Streptomyces KCCB_2 and Preparation of Antibiotics Using the Same Download PDF

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Publication number
KR900008034A
KR900008034A KR1019880015387A KR880015387A KR900008034A KR 900008034 A KR900008034 A KR 900008034A KR 1019880015387 A KR1019880015387 A KR 1019880015387A KR 880015387 A KR880015387 A KR 880015387A KR 900008034 A KR900008034 A KR 900008034A
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South Korea
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varidamycin
streptomyces
culture medium
kccb
purifying
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KR1019880015387A
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Korean (ko)
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KR910000454B1 (en
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이진우
한창훈
김보연
오영준
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김충세
고려화학 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Engineering & Computer Science (AREA)
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  • Biochemistry (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract

내용 없음No content

Description

스트렘토마이세스의 변이주 KCCB2및 이를 이용한 항생물질의 제조방법Variants of Streptomyces KCCB2 and Preparation of Antibiotics Using the Same

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (6)

스트렙토마이세스 하이그로스코피커스 변종 리모네우스 ATCC 21432의 변이주로서 당분 이용도가 높은 것을 특징으로 하는 스트렙토마이세스 하이그로스코피커스 변종 리모네우스 KCCB2(수탁번호 : KFCC - 10650)Streptomyces hygroscophys strain Limoneus KCCB 2 (Accession No .: KFCC-10650), characterized by high sugar utilization as a variant of the Streptomyces hygroscopic spp. 옥수수 전분 3중량%, 포도당 3 중량%, 설탕 3중량%, 옥수수침지액 3중량%, 코글루텐 3중량%, 면실분 3 중량%, 소금 1.5 중량%, 염화암모늄 0.5 중량%, 탄산칼슘 1.5 중량%를 함유하고 있으면서 pH 6.5 - 7.5인 배지에 스트렙토마이세스 하이그로스코커스 변종 리모네우스 KCCB2(수탁번호 : KFCC - 10650)의 종배양액을 5%부피 비율로 접종한후 교반속도 200rpmm 으로 28 - 38。C 에서 7일간 배양하여 배양액내에 바리다마이신A 를 축적시킨 다음, 이를 분리 정제하는 것을 특징으로 하는 바리다이마신A 의 제조방법.Corn starch 3%, glucose 3%, sugar 3%, corn steep 3%, gluten 3%, cottonseed flour 3%, salt 1.5%, ammonium chloride 0.5%, calcium carbonate 1.5% % Inoculated with a 5% volume ratio of a seed culture solution of Streptomyces hygroscocus strain Limoneus KCCB 2 (Accession No .: KFCC-10650) in a medium containing pH 6.5-7.5 and then stirred at 200 rpm with a stirring speed of 28-38 A method for producing varidamycin A, characterized by culturing at ° C for 7 days, accumulating varidamycin A in the culture medium, and then separating and purifying it. 제2항에 있어서, 배양시프로피온산, 유산, 능금산과 초산등의 유기산을 첨가하여 배양중의 배지 pH를 6.5 - 7.5로 유지하면서 배양액내에 바리다마이신A 를 축적시키는 것을 특징으로 하는 바리다마이신 의 제조방법.The method for producing varidamycin according to claim 2, wherein organic acids such as propionic acid, lactic acid, nitric acid and acetic acid are added to the culture medium to accumulate varidamycin A in the culture medium while maintaining the pH of the culture medium at 6.5 to 7.5. . 제2항에 있어서, 세포배양액의 균체분리시에는 한외과여과후에 생긴 농축액에 물을 가하고 이를 다시 여과하여 회수율 높이는 것을 특징으로 하는 바리다마이신 A의 여과방법.The method for filtration of varidamycin A according to claim 2, wherein the cell culture liquid is added with water to the concentrate obtained after ultrafiltration and filtered again to increase the recovery rate. 제2항에 있어서, 균체배양액의 정제분리시에는 양이온 교환수지에흡착후 용출시 증류수와 암모니아수의 농도기울기를 만들어 바리다마이신 A를 용출하는 것을 특징으로 하는 크로마토그라피법.The chromatographic method according to claim 2, wherein in purifying and separating the cell culture solution, varidamycin A is eluted by adsorbing to a cation exchange resin and eluting a concentration gradient between distilled water and ammonia water. 제5항에 있어서, 바리다마이신 A를 용출후 흡착 수지를 사용한 조색소 제거를 특징으로 하는 바리다마이신 A의 정제방법.The method for purifying varidamycin A according to claim 5, characterized in that crude pigment is removed using an adsorptive resin after eluting varidamycin A. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880015387A 1988-11-22 1988-11-22 Novel microorganism streptomyces sp kccb2 KR910000454B1 (en)

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KR1019880015387A KR910000454B1 (en) 1988-11-22 1988-11-22 Novel microorganism streptomyces sp kccb2

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Application Number Priority Date Filing Date Title
KR1019880015387A KR910000454B1 (en) 1988-11-22 1988-11-22 Novel microorganism streptomyces sp kccb2

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KR900008034A true KR900008034A (en) 1990-06-02
KR910000454B1 KR910000454B1 (en) 1991-01-25

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150071555A (en) * 2013-12-18 2015-06-26 동국제약 주식회사 A novel modified streptomyces filamentosus strain and the method for producing daptomycin using the same

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150071555A (en) * 2013-12-18 2015-06-26 동국제약 주식회사 A novel modified streptomyces filamentosus strain and the method for producing daptomycin using the same
US10301690B2 (en) 2013-12-18 2019-05-28 Dong Kook Pharm Co., Ltd. Streptomyces filamentosus variant and method for producing daptomycin using same

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KR910000454B1 (en) 1991-01-25

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