KR890006824A - Method for preparing L (-)-carnitine - Google Patents

Abstract

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Description

Method for preparing L (-)-carnitine

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Claims (13)

(a) culturing the strain under basic conditions in a culture solution containing (iii) D, L-cartinine and / or crotonobine, (ii) culturing the cultured enterobacteriaceae cells to obtain a crude protein extract, (Iii) chromatographically treat the crude protein extract with a column with a hydrophobic anion exchange resin as a support to separate and fix the cartinine hydrolase on the support, elute protein fractions and effect generators, and Filtration to obtain an effector-containing filtrate to separate the protein and the carotenoid hydrolase, and (b) a crotonobbetaine or crotonobbetaine derivative to react with a cartinine hydrolase in the presence of the effector, Produces L (-)-cartinine and (c) separates L (-)-cartinine from unreacted crotonobbetaine and effector, thereby producing crotonobbetaine or crotono By the three-dimensional characteristics Hydration the other derivatives, L process for preparing a non-Cartier - (-). The method according to claim 1, wherein the strain to be used is a strain of the genus Escherichia Proteus Citroacter and Salmonella. The strain according to claim 2, wherein the strain is Escherichia coli, Proteus vulgaris, Proteus mirabilis, Citrobacter freundii Salmonella typhimurium, Salmonella anatum (Salmonella anatum) and Salmonella cottbus (Salmonella cottbus) species of production method selected from. The method according to claim 3, wherein the strain of E. coli species is E. coli 044K74, E. coli 055K79, E. coli 0111K58 or E. coli 0114K90. The method according to claim 1, wherein the support of (a) (iii) is phenylsepharose, hydroxyapatite, DEAE-sepharose or DEAE-cellulose. The method according to claim 1, wherein the cartinine hydrolyase is fortified prior to the process (b). The method according to claim 1, wherein the carotenoid hydrolase to which the support is immobilized is activated by contact with a solution containing the effector. The method according to claim 1, wherein the carotenoid hydrolase immobilized on the support is activated by contact with a solution containing an effect generator and crotonobine or a derivative thereof. The method according to claim 1, wherein the solution containing 1 to 10 mmol of crotonobibetaine or a derivative thereof is contacted with a cartinine hydrolase at 24 ° C to 40 ° C, pH 6-9 in step (b). 10. The method according to claim 9, wherein a 5 mmol / 1 crotonovebetaine solution is introduced into a fixed cartinine hydrolase-containing column in the presence of an effector at a flow rate of 37 DEG C, 6 mL / hour, and pH 7.5. Manufacturing method. The method according to claim 1 or 9, wherein the process is carried out continuously. The method according to claim 1 or 9, wherein the unreacted crotonobine is separated from L (-)-cartinine and reused. 10. The method according to claim 1 or 9, wherein the crotonobine derivative is a crotonobine salt, crotonobbetanitrile, an ester of crotonobtaine or an amide of crotonobbetaine. ※ Note: The disclosure is based on the initial application.