KR890000659A - Plants resistant to stress and methods of mass production thereof - Google Patents

Abstract

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Description

Plants resistant to stress and methods of mass production thereof

Since this is an open matter, no full text was included.

1 is a flowchart of a method of making a plant resistant to stress in accordance with the principles of the present invention.

2 is a flowchart of a method of making a hormone suitable for inducing mononuclear cell proliferation from aberrant mononuclear cells in the proliferation of callus.

Claims (19)

(A) taking flakes from the plant selected as starting material, (B) exposing the starting material to the culture by the method of callus proliferation with aberrant mononuclear cells; (C) inducing mononuclear cell proliferation from aberrant mononuclear cells; (D) exposing the mononuclear cell proliferator to a certain level of a given stress condition until the death of most but not mononuclear cell proliferators; (E) inducing proliferation of the surviving single cell material obtained in step (D); (F) repeating steps (D) and (E) successively until the desired level of stress is reached; (G) Survival single species at each stage, starting with the viable single cell material obtained in step (F), until virtually all test chromosomes survive, repeatedly exposing the living single cell material to the desired level of stress. Inducing proliferation of cellular material; (H) inducing aberrant callus from the viable mononuclear cell proliferator obtained in step (G); And (I) inducing a callus proliferation from the abnormal callus obtained in step (H) as a method of producing a normal seedling, producing a strain of a selected plant that is resistant to a given stress condition. How to. The method according to claim 1, comprising a subsequent step of growing a plant resistant to stress from the seedlings. The method of claim 1, wherein the culture medium used in step (B) comprises growth hormone extracted from algae. The method according to claim 1, wherein a culture solution containing willow neoplasms is used during step (I) to promote callus lunch. The method of claim 3, wherein the culture used in step (B) comprises a mixture of banana and banana peel. The method of claim 4, wherein the culture used in step (I) comprises a mixture of banana and banana peel. The method of claim 3, wherein the growth hormone extracted from the algae, (B1) collecting algae, (B2) mechanically separating the collected algae along the stripes of the culture medium, (B3) Maintaining the culture and algae at a time and temperature that resists the stripes of species and mold, (B4) placing the single-celled algae in a dish containing sterile cultures that multiply single-species algae, and single-celled algae Method according to the method characterized in that consisting of mechanically fly to release the growth hormone. 8. The method according to claim 7, wherein the culture used contains agar. Plant obtained by the method of claim 1. Descendants of plants obtained by the method of claim 1. Fruit of the plant obtained by the method of claim 1. Seeds of plants obtained by the method of claim 1. The flower of the plant obtained by the method of claim 1. The method of claim 1, wherein the starting material is selected from the group comprising uncontaminated flowers, cultured tissue cultures, or the end of the selected plant. The method of any one of 1,2,3,4,5,6,7,8,9,10,11,12,13 or 14, wherein the given stress condition is cold. The method of any one of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14, wherein the given stress condition is heat. The method of any one of 1,2,3,4,5,6,7,8,9,10,11,12,13 or 14, wherein the given stress condition is salinity. The method of any one of 1,2,3,4,5,6,7,8,9,10,11,12,13 or 14, wherein the given stress condition is a pH level. The method of any one of 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14, wherein the given stress condition is a disease. ※ Note: The disclosure is based on the initial application.