KR870008021A - 시안화물 가수분해효소의 제조 - Google Patents

시안화물 가수분해효소의 제조 Download PDF

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KR870008021A
KR870008021A KR870001296A KR870001296A KR870008021A KR 870008021 A KR870008021 A KR 870008021A KR 870001296 A KR870001296 A KR 870001296A KR 870001296 A KR870001296 A KR 870001296A KR 870008021 A KR870008021 A KR 870008021A
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cyanide
strain
hydrolase
culture
hydrogen
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KR870001296A
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레인몬드 리차드슨 켄네쓰
모리스 클라크 피터
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원본미기재
임페리알 케미칼 인더스트리스 피엘씨
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Publication of KR870008021A publication Critical patent/KR870008021A/ko

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    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12R2001/77—Fusarium
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

내용 없음

Description

시안화물 가수분해효소의 제조
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
도면은 전체 활성에 따르는 시안화물 농도를 도시한 것이다.

Claims (10)

  1. (A) 최소한 15mM(배양물에 제공된 전체 배지내의 시안화 이온 및/또는 시안화 수소)과 동등한 농도에서 배양물의 발효조건하에 시안화 이온 및/또는 시안화 수소를 생성하는 화합물 및/또는 시안화 이온 및/또는 시안화 수소를 계속적으로 공급하면서 많아야 0.11hr-1인 희석율, 28-34℃ 온도 및 pH 4.5-7.5에서 적당한 무기영양분 및 탄소원을 포함하는 수성배양액내에 미생물 균주를 호기상태에서 계속적으로 배양하고 (B) 배양액으로부터 시안화물 가수분해효소를 포함하는 세포를 회수하는 단계로 구성되는, 시안화물 가수분해효소를 제조하는 방법.
  2. 제 1 항에 있어서, 미생물 균주가 Fusarium 균주인 방법.
  3. 제 2 항에 있어서, 균주가 Fusarium lateritium Nees 균주 CMI 300533 또는 그것의 돌연변이주 또는 변형주인 방법.
  4. 전기한 항의 어느 한 항에 있어서, 배양단계 (A)에서 탄소 또는 산소 제한 또는 이중의 탄소 및 산소 제한 조건하에 미생물 균주를 배양하는 방법.
  5. 전기한 항의 어느 한 항에 있어서, 탄소원이 포도당인 방법.
  6. 전기한 항의 어느 한 항에 있어서, 시안화 이온 및/또는 시안화 수소원이 2-10m mole/g 건조 세포중량인 전체 배지내의 농도에서 배양물에 제공된 방법.
  7. 제 6 항에 있어서, 시안화 이온 및/또는 시안화 수소원이 4-6m mole/g 건조 세포중량인 전체 배지내의 농도에서 배양물에 제공된 방법.
  8. 전기한 항의 어느 한 항에 있어서, PH 5.0-6.0 인 방법.
  9. 전기한 항의 어느 한 항에 있어서, 온도가 30-32℃인 방법.
  10. 본 발명에 따라 제조된 시안화물 가수분해효소로 구성되는 조성물 또는 시안화물 가수분해효소로 재료를 처리하는 것으로 구성되는, 시안화물을 포함하는 재료를 처리하는 시안화물을 분해하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR870001296A 1986-02-19 1987-02-17 시안화물 가수분해효소의 제조 KR870008021A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB8604068A GB8604068D0 (en) 1986-02-19 1986-02-19 Cyanide hydratase
GB04068 1986-02-19

Publications (1)

Publication Number Publication Date
KR870008021A true KR870008021A (ko) 1987-09-23

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ID=10593311

Family Applications (1)

Application Number Title Priority Date Filing Date
KR870001296A KR870008021A (ko) 1986-02-19 1987-02-17 시안화물 가수분해효소의 제조

Country Status (16)

Country Link
EP (1) EP0234760B1 (ko)
JP (1) JPS62224288A (ko)
KR (1) KR870008021A (ko)
CN (1) CN87100788A (ko)
AT (1) ATE80658T1 (ko)
AU (1) AU604675B2 (ko)
BR (1) BR8700711A (ko)
CA (1) CA1321967C (ko)
DE (1) DE3781701T2 (ko)
DK (1) DK82387A (ko)
FI (1) FI870674A (ko)
GB (2) GB8604068D0 (ko)
MY (1) MY101147A (ko)
NZ (1) NZ219330A (ko)
SU (1) SU1602394A3 (ko)
ZA (1) ZA87777B (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0233719A3 (en) * 1986-02-19 1988-07-06 Zeneca Limited Production of cyanide hydratase
GB9117379D0 (en) * 1991-08-12 1991-09-25 Ici Plc Purification of labile compounds
JPH0742705A (ja) * 1993-07-30 1995-02-10 Yutani Heavy Ind Ltd 作業機械の油圧装置
FR2737485B1 (fr) * 1995-08-02 1997-09-05 Pechiney Aluminium Procede et dispositif de traitement d'effluents contenant des cyanures
GB2314078B (en) * 1996-06-14 2000-06-07 British Gas Plc Biodegradation of iron cyanide complexes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0061249B1 (en) * 1981-03-20 1985-05-08 Imperial Chemical Industries Plc Effluent treatment
EP0233719A3 (en) * 1986-02-19 1988-07-06 Zeneca Limited Production of cyanide hydratase

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MY101147A (en) 1991-07-31
AU604675B2 (en) 1991-01-03
CN87100788A (zh) 1987-09-16
EP0234760A3 (en) 1988-06-29
FI870674A (fi) 1987-08-20
AU6854887A (en) 1987-08-20
BR8700711A (pt) 1987-12-22
DK82387D0 (da) 1987-02-18
ZA87777B (en) 1987-10-28
JPS62224288A (ja) 1987-10-02
CA1321967C (en) 1993-09-07
DE3781701D1 (de) 1992-10-22
DE3781701T2 (de) 1993-03-18
ATE80658T1 (de) 1992-10-15
EP0234760B1 (en) 1992-09-16
DK82387A (da) 1987-08-20
EP0234760A2 (en) 1987-09-02
NZ219330A (en) 1989-01-06
SU1602394A3 (ru) 1990-10-23
FI870674A0 (fi) 1987-02-18
GB8702137D0 (en) 1987-03-04
GB8604068D0 (en) 1986-03-26

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