KR860001826A - Polysaccharide RON substance, its manufacturing method and its usage - Google Patents

Polysaccharide RON substance, its manufacturing method and its usage Download PDF

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KR860001826A
KR860001826A KR1019850005958A KR850005958A KR860001826A KR 860001826 A KR860001826 A KR 860001826A KR 1019850005958 A KR1019850005958 A KR 1019850005958A KR 850005958 A KR850005958 A KR 850005958A KR 860001826 A KR860001826 A KR 860001826A
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ron
substance
solution containing
anion exchanger
treated
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KR1019850005958A
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KR870001930B1 (en
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스기루 다께오
히사오 야마모또
히사오 가또
나베 노부히로 와다
미노루 가미우라
기이찌 우찌다
야시따다 모리
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다까구와 요시다까
샷뽀로 비루 가부시기가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
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  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Molecular Biology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Epidemiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Materials Engineering (AREA)
  • Polymers & Plastics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

내용 없음No content

Description

다당체 RON물질, 그 제조법 및 그 사용법Polysaccharide RON substance, its manufacturing method and its usage

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 다당체 RON 물질의 자외부 흡수스펙트럼이다.1 is the ultraviolet absorption spectrum of the polysaccharide RON material.

제2도는 다당체 RON 물질의 적외부 흡수스펙트럼이다.2 is the infrared absorption spectrum of the polysaccharide RON material.

제3도는 다당체 RON 물질의 13 C-NMR 스펙트럼이다.3 is the 13 C-NMR spectrum of the polysaccharide RON material.

Claims (3)

쌀겨를 열수추출하여 얻어지는 추출랙에 극성유기용매 또는 염석제를 첨가하고, 생성한 침전물을 채취하여 필요에 따라서 정제처리를 실시함을 특징으로 한다. 글루코오스를 유일한 당구성 성분으로 하고, 직쇄상의1-6결합만으로 구성되고, 하기의 반복단위 및 제성질을 가지는 다당체 RON물질의 제조법.A polar organic solvent or a salting agent is added to an extraction rack obtained by hot water extraction of rice bran, and the resulting precipitate is collected and subjected to purification as necessary. With glucose as the only sugar component, linear A method for producing a polysaccharide RON material composed of only 1-6 bonds and having the following repeating units and properties. (1) 반복단위(1) repeat unit 6G 6G (단 G는 α-D-글루코피라노실기를 표시한다.)(Wherein G represents an α-D-glucopyranosyl group) (2) 투석막을 통과하지 않는다.(2) Do not pass through the dialysis membrane. (3) 알코올, 아세톤, 헥산, 벤젠, 초산에틸, 4염화탄소, 클로로포름 및 에테르에 불용이고, 물, 포름아미드 및 디메틸슬휙시드에 가용이다.(3) Insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, carbon tetrachloride, chloroform and ether and soluble in water, formamide and dimethylsulfide. (4) 1% 수용액은 중성이다.(4) 1% aqueous solution is neutral. (5) 하기의 원소분석치를 가진다.(5) It has the following elemental analysis value. C : 40.4-42.4%, H : 5.8-6.4% 회분 : 3.1-3.1%C: 40.4-42.4%, H: 5.8-6.4% Ash: 3.1-3.1% (6) 소량의 무기질(Si,P,K,Na,Ca,Mg,Cl 등)을 결합상태에서 함유한다.(6) It contains a small amount of inorganic matter (Si, P, K, Na, Ca, Mg, Cl, etc.) in the bonded state. (7) 하기의 정색반응을 나타낸다.(7) The following color reaction is shown. 안드론 유산반응, 훼노올 유산반응 및 크로모트로오프유산반응이 양성이고, 뷰레트반응, 로오리. 훠어링반응, 엘슨. 모으간 반응 및 요오드 반응이 각각음성이다.Andron lactic acid reactions, phenool lactic acid reactions and chromotrooff lactic acid reactions are positive, biuret reaction, loori. Coiling reaction, Elson. The pooled reaction and the iodine reaction are negative respectively. (8) 비선광도 := +142°-+145°(H2O)(8) Specific Radiance: = + 142 °-+ 145 ° (H 2 O) (9) 융점 : 명확한 융점을 표시하지 않고 220℃에서 갈변하고 230℃에서 흑변하여 탄화한다.(9) Melting point: Browning at 220 ℃ and blacking at 230 ℃ without marking clear melting point. (10) 제1도에 표시하는 자외부 흡수스펙트럼을 나타낸다.(10) The ultraviolet absorption spectrum shown in FIG. 1 is shown. (11) 제2도에 표시하는 적외부 흡수스펙트럼을 표시한다.(11) The infrared absorption spectrum shown in FIG. 2 is displayed. (12) 제3도에 표시하는13C-NMR 스펙트럼을 표시하는데 하기사항을 포함한다.(12) The following items are included to display the 13 C-NMR spectrum shown in FIG. 쌀겨를 상술한 다당체 RON물질을 추출하기 위하여 열수처리하고,α-아밀라아제외/또는 프로티나아제와 함께 전분과/혹은 단백질을 제거하기 위하여 RON물질을 함유하는 추출물이 처리되고 극성유기용매 또는 염석제를 침전물로서의 RON물질을 다시 찾기 위하여 RON물질을 함유하는 추출물에 첨가하고, 침전물을 물에서 용해하고 이와같이 얻은 RON물질을 포함하는 용액을 울트라여과 또는 겔여과 또는 유수에 대한 투석에 의하여 처리하고, 상술한 RON물질을 함유하는 용액은 상술한 음이온 교환체에 흡착되어 있지 않는 획분(fraction)을 얻기위하여 음이온교환체로 처리되고, 상술한 음이온교환체로 처리된 RON물질을 함유하는 용액으로부터 RON물질의 백색분말을 다시 찾는 과정을 포함한다.Rice bran is hydrothermally treated to extract the above-mentioned polysaccharide RON substance, and an extract containing RON substance is treated to remove starch and / or protein together with α-amylase and / or proteinase, and is a polar organic solvent or salting agent. Is added to the extract containing RON material to find RON material as a precipitate again, the precipitate is dissolved in water and the solution containing the RON material thus obtained is treated by ultrafiltration or gel filtration or dialysis against running water, A solution containing one RON substance is treated with an anion exchanger to obtain a fraction that is not adsorbed to the anion exchanger described above, and a white powder of the RON substance from a solution containing the RON substance treated with the anion exchanger described above. It involves the process of finding it again. 청구범위 제1항에 있어서 상술한 RON물질을 함유하는 용액에 상술한 음이온 교환체를 첨가하고, 상술한 음이온 교환체가 흡착되어 있지 않는 용액을 얻기 위하여 1~2시간 교반한 후의 혼합물로부터 RON 물질을 다시 찾아냄으로서 실시되는 음이온 교환체에 의한 처리를 포함하는 다당체 RON의 제조방법.The RON substance is added from the mixture after stirring for 1 to 2 hours to add the above-mentioned anion exchanger to the solution containing the above-mentioned RON substance, and to obtain the solution which the above-mentioned anion exchanger does not adsorb | suck. A method for producing a polysaccharide RON comprising a treatment with an anion exchanger carried out by rediscovering. 청구범위 제1항에 있어서 RON물질을 함유하는 용액이 RON 물질을 침전시키기 위하여 RON물질을 함유하는 용액을 동결건조 또는 분무건조하거나 혹은 극성유기용매에 의하여 실시되는 RON물질의 재회수 과정을 포함하는 다당체 RON의제조방법.The method according to claim 1, wherein the solution containing the RON material comprises a process for re-recovering the RON material carried out by lyophilization or spray drying of the solution containing the RON material or by polar organic solvent to precipitate the RON material. Method of producing polysaccharide RON. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019850005958A 1959-08-22 1985-08-19 Preparation method of polysaccharides,ron KR870001930B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP??87-173168 1959-08-22
JP84.173168 1984-08-22
JP59173168A JPS6153220A (en) 1984-08-22 1984-08-22 Novel polysaccharide ron substance, its preparation and antitumor agent, immuno-regulating agent and preventive and remedy for infectious disease, containing said substance as active component

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KR860001826A true KR860001826A (en) 1986-03-22
KR870001930B1 KR870001930B1 (en) 1987-10-22

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US (1) US4762825A (en)
EP (1) EP0172559B1 (en)
JP (1) JPS6153220A (en)
KR (1) KR870001930B1 (en)
CA (1) CA1282779C (en)
DE (1) DE3581981D1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332667A (en) * 1989-02-08 1994-07-26 Sapporo Breweries Limited Method for producing biologically active polysaccharide RON substance
CA2009194A1 (en) * 1989-02-08 1990-08-08 Hideo Kado Method for producing biologically active polysaccharide ron substance
JPH0740953B2 (en) * 1989-02-08 1995-05-10 サッポロビール株式会社 Method for producing bioactive polysaccharide RON
JP2772813B2 (en) * 1989-03-03 1998-07-09 サッポロビール株式会社 Composition having antiviral action and immunostimulatory action
JPH0678367B2 (en) * 1990-05-15 1994-10-05 呉羽化学工業株式会社 Dietary fiber, method for producing the same, and bioactive agent containing the dietary fiber
US5466680A (en) * 1992-03-26 1995-11-14 Cytologics, Inc. Method and compositions for enhancing white blood cell functioning on a mucosal or cutaneous surface
JP2583183B2 (en) * 1993-02-12 1997-02-19 青森県 Process for producing bioactive glycogen and bioactivity
US20040166248A1 (en) * 2000-12-15 2004-08-26 Sheng-Hsin Hu Coated activated carbon
US6740406B2 (en) 2000-12-15 2004-05-25 Kimberly-Clark Worldwide, Inc. Coated activated carbon
CN100435805C (en) * 2003-05-26 2008-11-26 福州大学 Use of polysaccharose sulfate for preparing medicine against grippal virus

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5943929B2 (en) * 1979-08-13 1984-10-25 サッポロビール株式会社 Polysaccharide RBS substance, its production method, and antitumor agent containing it as an active ingredient
JPS5936964B2 (en) * 1980-11-04 1984-09-06 サッポロビール株式会社 immunostimulant
JPS59170017A (en) * 1983-03-18 1984-09-26 Sapporo Breweries Ltd Novel polysaccharide rdp substance, its preparation, antitumor agent, immune adjusting agent, preventive and remedy for infectious disease containing it as active ingredient

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CA1282779C (en) 1991-04-09
EP0172559A2 (en) 1986-02-26
US4762825A (en) 1988-08-09
KR870001930B1 (en) 1987-10-22
DE3581981D1 (en) 1991-04-11
JPS627173B2 (en) 1987-02-16
EP0172559B1 (en) 1991-03-06
JPS6153220A (en) 1986-03-17
EP0172559A3 (en) 1987-06-24

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