KR20250168544A - 표적 뉴클레오티드 서열의 개변을 위한 비천연형 폴리뉴클레오티드 - Google Patents

표적 뉴클레오티드 서열의 개변을 위한 비천연형 폴리뉴클레오티드

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Publication number
KR20250168544A
KR20250168544A KR1020257036092A KR20257036092A KR20250168544A KR 20250168544 A KR20250168544 A KR 20250168544A KR 1020257036092 A KR1020257036092 A KR 1020257036092A KR 20257036092 A KR20257036092 A KR 20257036092A KR 20250168544 A KR20250168544 A KR 20250168544A
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South Korea
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nucleotide
nucleotides
natural polynucleotide
geo
nucleic acid
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Pending
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KR1020257036092A
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English (en)
Korean (ko)
Inventor
다카토 노우미
게이코 하야미
나오키 이노우에
Original Assignee
유러스 세러퓨틱스 가부시키가이샤
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Publication of KR20250168544A publication Critical patent/KR20250168544A/ko
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
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    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/712—Nucleic acids or oligonucleotides having modified sugars, i.e. other than ribose or 2'-deoxyribose
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7125—Nucleic acids or oligonucleotides having modified internucleoside linkage, i.e. other than 3'-5' phosphodiesters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
    • C12N2310/3525—MOE, methoxyethoxy
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Health & Medical Sciences (AREA)
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  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Organic Chemistry (AREA)
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  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020257036092A 2023-04-26 2024-04-26 표적 뉴클레오티드 서열의 개변을 위한 비천연형 폴리뉴클레오티드 Pending KR20250168544A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2023-071927 2023-04-26
JP2023071927 2023-04-26
PCT/JP2024/016395 WO2024225425A1 (ja) 2023-04-26 2024-04-26 標的ヌクレオチド配列の改変のための非天然型ポリヌクレオチド

Publications (1)

Publication Number Publication Date
KR20250168544A true KR20250168544A (ko) 2025-12-02

Family

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Family Applications (1)

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KR1020257036092A Pending KR20250168544A (ko) 2023-04-26 2024-04-26 표적 뉴클레오티드 서열의 개변을 위한 비천연형 폴리뉴클레오티드

Country Status (7)

Country Link
EP (1) EP4703472A1 (https=)
JP (2) JP7660960B2 (https=)
KR (1) KR20250168544A (https=)
CN (1) CN121443735A (https=)
AU (1) AU2024260090A1 (https=)
IL (1) IL324176A (https=)
WO (1) WO2024225425A1 (https=)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5405121B2 (ja) 2005-12-22 2014-02-05 キージーン ナムローゼ フェンノートシャップ 二重鎖アクセプターｄｎａ塩基配列の標的化改変のための方法
JP6206893B2 (ja) 2014-03-05 2017-10-04 国立大学法人神戸大学 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体
JP6343605B2 (ja) 2012-05-25 2018-06-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物
JP6480647B1 (ja) 2017-06-08 2019-03-13 国立大学法人大阪大学 Ｄｎａが編集された真核細胞を製造する方法、および当該方法に用いられるキット

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006020560A (ja) * 2004-07-07 2006-01-26 National Institute Of Agrobiological Sciences 核酸を配列特異的に標識する方法、およびそれを利用した新規核酸検出法
CN102016035A (zh) * 2006-11-02 2011-04-13 犹他大学研究基金会 可用于等位基因特异pcr的寡聚核苷酸
EP2167660A1 (en) * 2007-06-22 2010-03-31 Keygene N.V. Targeted nucleotide exchange with improved modified oligonucleotides
EP2970979B1 (en) * 2013-03-14 2018-11-21 Somalogic, Inc. Aptamers that bind to il-6 and their use in treating or diagnosing il-6 mediated conditions
EP4545649A3 (en) * 2016-11-11 2025-06-04 Bio-Rad Laboratories, Inc. Methods for processing nucleic acid samples
EP4347818A2 (en) * 2021-06-01 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5405121B2 (ja) 2005-12-22 2014-02-05 キージーン ナムローゼ フェンノートシャップ 二重鎖アクセプターｄｎａ塩基配列の標的化改変のための方法
JP6343605B2 (ja) 2012-05-25 2018-06-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物
JP6206893B2 (ja) 2014-03-05 2017-10-04 国立大学法人神戸大学 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体
JP6480647B1 (ja) 2017-06-08 2019-03-13 国立大学法人大阪大学 Ｄｎａが編集された真核細胞を製造する方法、および当該方法に用いられるキット

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Thomas W. van Ravesteyn, Marcos Arranz Dols, Wietske Pieters, Marleen Dekker, Hein te Riele (2020) Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells. PLoS Genet 16 (10) : e1009041.https://doi.org/10.1371/journal.pgen.1009041
Thomas W. van Ravesteyn, Marleen Dekker, Alexander Fish, Titia K. Sixma, Astrid Wolters, Rob J. Dekker, and Hein P. J. te Riele (2016) LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells, PNAS Vol.113, no.15, 4122-4127

Also Published As

Publication number Publication date
AU2024260090A1 (en) 2025-12-04
IL324176A (en) 2025-12-01
EP4703472A1 (en) 2026-03-04
WO2024225425A1 (ja) 2024-10-31
JPWO2024225425A1 (https=) 2024-10-31
JP7660960B2 (ja) 2025-04-14
JP2025092562A (ja) 2025-06-19
CN121443735A (zh) 2026-01-30

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