KR20240169697A - 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법 - Google Patents

올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법 Download PDF

Info

Publication number
KR20240169697A
KR20240169697A KR1020247036719A KR20247036719A KR20240169697A KR 20240169697 A KR20240169697 A KR 20240169697A KR 1020247036719 A KR1020247036719 A KR 1020247036719A KR 20247036719 A KR20247036719 A KR 20247036719A KR 20240169697 A KR20240169697 A KR 20240169697A
Authority
KR
South Korea
Prior art keywords
polynucleotide
fragment
product
oligonucleotide
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247036719A
Other languages
English (en)
Korean (ko)
Inventor
안드레아스 크라메리
더글라스 퓌르스트
조셉 호스포드
데이비드 튜
Original Assignee
글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드 filed Critical 글락소스미스클라인 인털렉츄얼 프로퍼티 디벨로프먼트 리미티드
Publication of KR20240169697A publication Critical patent/KR20240169697A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020247036719A 2022-04-08 2023-04-06 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법 Pending KR20240169697A (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263328912P 2022-04-08 2022-04-08
US63/328,912 2022-04-08
US202263432466P 2022-12-14 2022-12-14
US63/432,466 2022-12-14
PCT/EP2023/059142 WO2023194537A1 (en) 2022-04-08 2023-04-06 Novel processes for the production of polynucleotides including oligonucleotides

Publications (1)

Publication Number Publication Date
KR20240169697A true KR20240169697A (ko) 2024-12-03

Family

ID=85985237

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247036719A Pending KR20240169697A (ko) 2022-04-08 2023-04-06 올리고뉴클레오티드를 포함한 폴리뉴클레오티드의 신규 생산 방법

Country Status (8)

Country Link
US (1) US20250223620A1 (https=)
EP (1) EP4504932A1 (https=)
JP (1) JP2025511938A (https=)
KR (1) KR20240169697A (https=)
CN (1) CN119013398A (https=)
IL (1) IL315519A (https=)
TW (1) TW202405168A (https=)
WO (1) WO2023194537A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202340468A (zh) 2022-01-10 2023-10-16 中國大陸商杭州浩博醫藥有限公司 B型肝炎病毒(hbv)表現之調節
CN118460651B (zh) * 2024-07-10 2025-03-18 凯莱英医药集团（天津）股份有限公司 制备Cemdisiran的方法
CN118460654B (zh) * 2024-07-10 2025-02-14 凯莱英医药集团（天津）股份有限公司 一种Givosiran的制备方法
CN118460653B (zh) * 2024-07-10 2024-09-24 凯莱英医药集团（天津）股份有限公司 一种治疗α1-抗胰蛋白酶缺乏症的siRNA的制备方法
CN119876080B (zh) * 2025-01-15 2025-11-07 北京擎科生物科技股份有限公司 一种kod dna聚合酶突变体及其生产方法和应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6683173B2 (en) 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US6586586B1 (en) 2000-01-31 2003-07-01 Isis Pharmaceuticals, Inc. Purification of oligonucleotides
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
GB201115218D0 (en) 2011-09-02 2011-10-19 Glaxo Group Ltd Novel process
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
AU2016341309A1 (en) * 2015-10-22 2018-06-07 Modernatx, Inc. Cancer vaccines
EP3393525A4 (en) * 2015-12-21 2019-08-21 Gen9, Inc. METHODS AND COMPOSITIONS FOR ASSEMBLING NUCLEIC ACIDS
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
JP2020507312A (ja) * 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
GB201721307D0 (en) 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides

Also Published As

Publication number Publication date
TW202405168A (zh) 2024-02-01
JP2025511938A (ja) 2025-04-16
EP4504932A1 (en) 2025-02-12
US20250223620A1 (en) 2025-07-10
CN119013398A (zh) 2024-11-22
WO2023194537A1 (en) 2023-10-12
IL315519A (en) 2024-11-01

Similar Documents

Publication Publication Date Title
US20250223620A1 (en) Novel processes for the production of polynucleotides including oligonucleotides
JP7438259B2 (ja) オリゴヌクレオチドの産生のための新規プロセス
KR102205974B1 (ko) 올리고뉴클레오티드의 생성을 위한 신규한 방법
JP2024534945A (ja) 化学修飾を有するプライム編集のためのガイドｒｎａ
US20090298708A1 (en) 2'-deoxy-2'-fluoro-beta-d-arabinonucleoside 5'-triphosphates and their use in enzymatic nucleic acid synthesis
WO2025061911A1 (en) Methods of producing nucleotide triphosphates (ntps)
CN121986172A (en) Method for producing Nucleoside Triphosphates (NTPs)
BR112018076725B1 (pt) Processos para produzir um produto de oligonucleotídeo de fita simples e de fita dupla

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000