CN119013398A - 生产包括寡核苷酸的多核苷酸的新方法 - Google Patents

生产包括寡核苷酸的多核苷酸的新方法 Download PDF

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Publication number
CN119013398A
CN119013398A CN202380031791.1A CN202380031791A CN119013398A CN 119013398 A CN119013398 A CN 119013398A CN 202380031791 A CN202380031791 A CN 202380031791A CN 119013398 A CN119013398 A CN 119013398A
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CN
China
Prior art keywords
polynucleotide
fragment
product
oligonucleotide
template
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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CN202380031791.1A
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English (en)
Chinese (zh)
Inventor
A·克拉梅利
D·福耶斯特
J·霍斯福德
D·蒂乌
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GlaxoSmithKline Intellectual Property Development Ltd
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GlaxoSmithKline Intellectual Property Development Ltd
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Publication of CN119013398A publication Critical patent/CN119013398A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202380031791.1A 2022-04-08 2023-04-06 生产包括寡核苷酸的多核苷酸的新方法 Pending CN119013398A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263328912P 2022-04-08 2022-04-08
US63/328,912 2022-04-08
US202263432466P 2022-12-14 2022-12-14
US63/432,466 2022-12-14
PCT/EP2023/059142 WO2023194537A1 (en) 2022-04-08 2023-04-06 Novel processes for the production of polynucleotides including oligonucleotides

Publications (1)

Publication Number Publication Date
CN119013398A true CN119013398A (zh) 2024-11-22

Family

ID=85985237

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202380031791.1A Pending CN119013398A (zh) 2022-04-08 2023-04-06 生产包括寡核苷酸的多核苷酸的新方法

Country Status (8)

Country Link
US (1) US20250223620A1 (https=)
EP (1) EP4504932A1 (https=)
JP (1) JP2025511938A (https=)
KR (1) KR20240169697A (https=)
CN (1) CN119013398A (https=)
IL (1) IL315519A (https=)
TW (1) TW202405168A (https=)
WO (1) WO2023194537A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119876080A (zh) * 2025-01-15 2025-04-25 北京擎科生物科技股份有限公司 一种kod dna聚合酶突变体及其生产方法和应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202340468A (zh) 2022-01-10 2023-10-16 中國大陸商杭州浩博醫藥有限公司 B型肝炎病毒(hbv)表現之調節
CN118460651B (zh) * 2024-07-10 2025-03-18 凯莱英医药集团（天津）股份有限公司 制备Cemdisiran的方法
CN118460654B (zh) * 2024-07-10 2025-02-14 凯莱英医药集团（天津）股份有限公司 一种Givosiran的制备方法
CN118460653B (zh) * 2024-07-10 2024-09-24 凯莱英医药集团（天津）股份有限公司 一种治疗α1-抗胰蛋白酶缺乏症的siRNA的制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6683173B2 (en) 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US6586586B1 (en) 2000-01-31 2003-07-01 Isis Pharmaceuticals, Inc. Purification of oligonucleotides
AU2001241939A1 (en) * 2000-02-28 2001-09-12 Maxygen, Inc. Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation
GB201115218D0 (en) 2011-09-02 2011-10-19 Glaxo Group Ltd Novel process
US8808989B1 (en) 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
GB201502152D0 (en) 2015-02-10 2015-03-25 Nuclera Nucleics Ltd Novel use
GB201503534D0 (en) 2015-03-03 2015-04-15 Nuclera Nucleics Ltd Novel method
AU2016341309A1 (en) * 2015-10-22 2018-06-07 Modernatx, Inc. Cancer vaccines
EP3393525A4 (en) * 2015-12-21 2019-08-21 Gen9, Inc. METHODS AND COMPOSITIONS FOR ASSEMBLING NUCLEIC ACIDS
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
JP2020507312A (ja) * 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
GB201721307D0 (en) 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119876080A (zh) * 2025-01-15 2025-04-25 北京擎科生物科技股份有限公司 一种kod dna聚合酶突变体及其生产方法和应用

Also Published As

Publication number Publication date
TW202405168A (zh) 2024-02-01
JP2025511938A (ja) 2025-04-16
EP4504932A1 (en) 2025-02-12
US20250223620A1 (en) 2025-07-10
WO2023194537A1 (en) 2023-10-12
IL315519A (en) 2024-11-01
KR20240169697A (ko) 2024-12-03

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