KR20240134152A - 대량 세포 용해에 내성이 있는 유전자 변형 박테리아 - Google Patents

대량 세포 용해에 내성이 있는 유전자 변형 박테리아 Download PDF

Info

Publication number
KR20240134152A
KR20240134152A KR1020247025904A KR20247025904A KR20240134152A KR 20240134152 A KR20240134152 A KR 20240134152A KR 1020247025904 A KR1020247025904 A KR 1020247025904A KR 20247025904 A KR20247025904 A KR 20247025904A KR 20240134152 A KR20240134152 A KR 20240134152A
Authority
KR
South Korea
Prior art keywords
polypeptide
seq
amino acid
gene
bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247025904A
Other languages
English (en)
Korean (ko)
Inventor
데이비드 바이런트
알렉산더 요하네스 크루이스
토니 나고데
그레고르 코세츠
Original Assignee
에이시에스 바이오 디.오.오.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 에이시에스 바이오 디.오.오. filed Critical 에이시에스 바이오 디.오.오.
Publication of KR20240134152A publication Critical patent/KR20240134152A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01184—Acyl-homoserine-lactone synthase (2.3.1.184)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020247025904A 2022-01-21 2023-01-23 대량 세포 용해에 내성이 있는 유전자 변형 박테리아 Pending KR20240134152A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP22152702.1A EP4215601A1 (en) 2022-01-21 2022-01-21 Genetically modified bacteria resistant to mass cell lysis
EP22152702.1 2022-01-21
PCT/EP2023/051537 WO2023139255A1 (en) 2022-01-21 2023-01-23 Genetically modified bacteria resistant to mass cell lysis

Publications (1)

Publication Number Publication Date
KR20240134152A true KR20240134152A (ko) 2024-09-06

Family

ID=80447330

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247025904A Pending KR20240134152A (ko) 2022-01-21 2023-01-23 대량 세포 용해에 내성이 있는 유전자 변형 박테리아

Country Status (7)

Country Link
US (1) US20250270603A1 (https=)
EP (2) EP4215601A1 (https=)
JP (1) JP2025508627A (https=)
KR (1) KR20240134152A (https=)
CN (1) CN118679240A (https=)
CA (1) CA3241975A1 (https=)
WO (1) WO2023139255A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4552507A1 (en) 2023-11-10 2025-05-14 Acies Bio d.o.o. Methylotroph bacteria as food and feed ingredients
WO2026003194A1 (en) 2024-06-27 2026-01-02 Basf Se Production of c6-c26 fatty alcohols from methanol
WO2026003192A2 (en) 2024-06-27 2026-01-02 Basf Se Production of c6-c26 fatty acids from methanol

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JP2001120269A (ja) * 1999-10-22 2001-05-08 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法

Also Published As

Publication number Publication date
JP2025508627A (ja) 2025-04-02
US20250270603A1 (en) 2025-08-28
CA3241975A1 (en) 2023-07-27
EP4215601A1 (en) 2023-07-26
CN118679240A (zh) 2024-09-20
WO2023139255A1 (en) 2023-07-27
EP4466341A1 (en) 2024-11-27

Similar Documents

Publication Publication Date Title
KR20240134152A (ko) 대량 세포 용해에 내성이 있는 유전자 변형 박테리아
Feng et al. Improved poly-γ-glutamic acid production in Bacillus amyloliquefaciens by modular pathway engineering
CA2700510A1 (en) Mutant microorganisms having high ability to produce putrescine and method for producing putrescine using the same
JP7753355B2 (ja) 改善特性を有する遺伝子改変メチロバチルス細菌
US20250163478A1 (en) Bioconversion of levulinic acid in genetically engineered hosts
JP5140848B2 (ja) 没食子酸の製造法
JP7464921B2 (ja) ヘパロサンの製造方法及びヘパロサン生産能を有するエシェリヒア属細菌
Perlova et al. Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2
WO2022013663A1 (en) 5-methylfolate producing microorganism
KR102857796B1 (ko) 헤파로산의 제조 방법 및 헤파로산 생산능을 갖는 에스케리키아 속의 세균
RU2823674C1 (ru) Способ продукции гепаросана и бактерия из рода escherichia, имеющая способность к продукции гепаросана
US20250263731A1 (en) Genetically modified methylotrophic bacteria producing lactate
KR101533290B1 (ko) Ｄ―갈락토네이트 고생산 대장균 균주 및 이의 용도
KR101533150B1 (ko) 모듈식 스캐폴드를 이용한 감마 아미노부틸산 생산방법
KR101541590B1 (ko) 모듈식 스캐폴드를 이용한 감마 아미노부틸산 생산방법
CN112673016B (zh) 用于改善的木糖利用或改善的葡萄糖和木糖共利用的xylr突变体
Hernandez Investigation of the Effects of Stress Related Genes on Escherichia coli Fermentation
CN113677795A (zh) 新型dahp合成酶
Wang Optimization of Fermentation Conditions for the Production of Legionaminic Acid in Recombinant Escherichia coli

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20240731

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application