JP2025508627A - 大量細胞溶解に耐性のある遺伝子改変された細菌 - Google Patents

大量細胞溶解に耐性のある遺伝子改変された細菌 Download PDF

Info

Publication number
JP2025508627A
JP2025508627A JP2024542265A JP2024542265A JP2025508627A JP 2025508627 A JP2025508627 A JP 2025508627A JP 2024542265 A JP2024542265 A JP 2024542265A JP 2024542265 A JP2024542265 A JP 2024542265A JP 2025508627 A JP2025508627 A JP 2025508627A
Authority
JP
Japan
Prior art keywords
polypeptide
seq
amino acid
bacterium
sequence identity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2024542265A
Other languages
English (en)
Japanese (ja)
Other versions
JP2025508627A5 (https=
Inventor
ヴィラント,デイヴィッド
ヨハネス クルイス,アレクサンダー
ナゴデ,トニ
コーセック,グレゴール
Original Assignee
アキエス バイオ ディー.オー.オー.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by アキエス バイオ ディー.オー.オー. filed Critical アキエス バイオ ディー.オー.オー.
Publication of JP2025508627A publication Critical patent/JP2025508627A/ja
Publication of JP2025508627A5 publication Critical patent/JP2025508627A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1229Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/005Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01184Acyl-homoserine-lactone synthase (2.3.1.184)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001Polyphosphate kinase (2.7.4.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2024542265A 2022-01-21 2023-01-23 大量細胞溶解に耐性のある遺伝子改変された細菌 Pending JP2025508627A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP22152702.1A EP4215601A1 (en) 2022-01-21 2022-01-21 Genetically modified bacteria resistant to mass cell lysis
EP22152702.1 2022-01-21
PCT/EP2023/051537 WO2023139255A1 (en) 2022-01-21 2023-01-23 Genetically modified bacteria resistant to mass cell lysis

Publications (2)

Publication Number Publication Date
JP2025508627A true JP2025508627A (ja) 2025-04-02
JP2025508627A5 JP2025508627A5 (https=) 2026-01-30

Family

ID=80447330

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024542265A Pending JP2025508627A (ja) 2022-01-21 2023-01-23 大量細胞溶解に耐性のある遺伝子改変された細菌

Country Status (7)

Country Link
US (1) US20250270603A1 (https=)
EP (2) EP4215601A1 (https=)
JP (1) JP2025508627A (https=)
KR (1) KR20240134152A (https=)
CN (1) CN118679240A (https=)
CA (1) CA3241975A1 (https=)
WO (1) WO2023139255A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4552507A1 (en) 2023-11-10 2025-05-14 Acies Bio d.o.o. Methylotroph bacteria as food and feed ingredients
WO2026003194A1 (en) 2024-06-27 2026-01-02 Basf Se Production of c6-c26 fatty alcohols from methanol
WO2026003192A2 (en) 2024-06-27 2026-01-02 Basf Se Production of c6-c26 fatty acids from methanol

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
JP2001120269A (ja) * 1999-10-22 2001-05-08 Ajinomoto Co Inc 発酵法によるl−リジンの製造法

Also Published As

Publication number Publication date
US20250270603A1 (en) 2025-08-28
CA3241975A1 (en) 2023-07-27
EP4215601A1 (en) 2023-07-26
CN118679240A (zh) 2024-09-20
KR20240134152A (ko) 2024-09-06
WO2023139255A1 (en) 2023-07-27
EP4466341A1 (en) 2024-11-27

Similar Documents

Publication Publication Date Title
Liang et al. Synechocystis PCC 6803 overexpressing RuBisCO grow faster with increased photosynthesis
JP2025508627A (ja) 大量細胞溶解に耐性のある遺伝子改変された細菌
Kim et al. Down‐regulation of acetate pathway through antisense strategy in Escherichia coli: Improved foreign protein production
CN108291231B (zh) 由3-羟基丙酸诱导表达的启动子系统及用其生物生产3-羟基丙酸的方法
CN107406864B (zh) 用于使用缺乏丝氨酸降解途径的基因工程微生物生产l-丝氨酸的方法
EP3158065B1 (en) Nucleic acids and vectors for use with methanotrophic bacteria
WO2011140342A1 (en) Mutations and genetic targets for enhanced l-tyrosine production
US20130210097A1 (en) Glycolic acid fermentative production with a modified microorganism
JP7753355B2 (ja) 改善特性を有する遺伝子改変メチロバチルス細菌
KR20210015881A (ko) 메타크릴레이트 제조 방법
EP3068887A1 (en) Bacteria with improved metabolic capacity
Rodríguez-Moya et al. Involvement of EupR, a response regulator of the NarL/FixJ family, in the control of the uptake of the compatible solutes ectoines by the halophilic bacterium Chromohalobacter salexigens
CN115605602A (zh) 用于生产肝素原的方法和具有肝素原生产能力的埃希氏杆菌属细菌
KR101541034B1 (ko) D―갈락토네이트 고생산 대장균 균주 및 이의 용도
KR101863239B1 (ko) 아세트산을 유일 탄소원으로 이용할 수 있는 미생물
KR101785150B1 (ko) 모듈식 스캐폴드를 이용한 감마 아미노부티르산의 생산방법
WO2022013663A1 (en) 5-methylfolate producing microorganism
KR101533290B1 (ko) D―갈락토네이트 고생산 대장균 균주 및 이의 용도
RU2823674C1 (ru) Способ продукции гепаросана и бактерия из рода escherichia, имеющая способность к продукции гепаросана
KR102857796B1 (ko) 헤파로산의 제조 방법 및 헤파로산 생산능을 갖는 에스케리키아 속의 세균
US20250263731A1 (en) Genetically modified methylotrophic bacteria producing lactate
CN113677795B (zh) 新型dahp合成酶
Zhang et al. High-level prodigiosin production in Pseudomonas putida enabled by combinatorial metabolic engineering
San et al. Galactose utilization
Hernandez Investigation of the Effects of Stress Related Genes on Escherichia coli Fermentation

Legal Events

Date Code Title Description
A529 Written submission of copy of amendment under article 34 pct

Free format text: JAPANESE INTERMEDIATE CODE: A529

Effective date: 20240912

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250318

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20260122

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20260122