KR20240126447A - 원형 dna 벡터의 합성 생산 - Google Patents

원형 dna 벡터의 합성 생산 Download PDF

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Publication number
KR20240126447A
KR20240126447A KR1020247014254A KR20247014254A KR20240126447A KR 20240126447 A KR20240126447 A KR 20240126447A KR 1020247014254 A KR1020247014254 A KR 1020247014254A KR 20247014254 A KR20247014254 A KR 20247014254A KR 20240126447 A KR20240126447 A KR 20240126447A
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South Korea
Prior art keywords
therapeutic
dna
linear
sequence
dna vector
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KR1020247014254A
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English (en)
Korean (ko)
Inventor
진 허
아일린 히그햄
조세 로라
조디 케네디
앤 매과이어
메이삼 바흐샤이쉬
일라이자 돈부쉬
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인터갈락틱 테라퓨틱스, 인크.
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Publication of KR20240126447A publication Critical patent/KR20240126447A/ko
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
KR1020247014254A 2021-09-27 2022-09-27 원형 dna 벡터의 합성 생산 Pending KR20240126447A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163248801P 2021-09-27 2021-09-27
US63/248,801 2021-09-27
PCT/US2022/077108 WO2023049937A2 (en) 2021-09-27 2022-09-27 Synthetic production of circular dna vectors

Publications (1)

Publication Number Publication Date
KR20240126447A true KR20240126447A (ko) 2024-08-20

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Application Number Title Priority Date Filing Date
KR1020247014254A Pending KR20240126447A (ko) 2021-09-27 2022-09-27 원형 dna 벡터의 합성 생산

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Country Link
US (1) US20240409975A1 (enExample)
EP (1) EP4408994A4 (enExample)
JP (1) JP2024535917A (enExample)
KR (1) KR20240126447A (enExample)
CN (1) CN118679248A (enExample)
AU (1) AU2022349697A1 (enExample)
CA (1) CA3233230A1 (enExample)
IL (1) IL311778A (enExample)
MX (1) MX2024003870A (enExample)
WO (1) WO2023049937A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026515898A (ja) * 2023-04-28 2026-05-19 ユニバーシティ オブ メリーランド、バルティモア カウンティ インビトロでスーパーコイル状の環状ｄｎａを作製する方法
WO2025007021A2 (en) * 2023-06-30 2025-01-02 Novel Biotechnology Inc. Cell-free replication of a nucleic acid construct
WO2025175112A1 (en) * 2024-02-16 2025-08-21 University Of Maryland, Baltimore County Method for generating circular dna using heteroduplex thermostable ligation assembly of precursors produced by rolling circle amplification

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE340264T1 (de) * 2003-06-10 2006-10-15 Mologen Ag Zirkuläres expressionskonstrukt für gentherapeutische anwendungen
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
GB201321123D0 (en) * 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
JP7058502B2 (ja) * 2014-04-11 2022-04-22 レッドボールト バイオサイエンシズ エルピー ゲノム適用および治療適用のための、核酸分子のクローン複製および増幅のためのシステムおよび方法
FR3032447B1 (fr) * 2015-02-10 2020-02-21 Universite Des Sciences Et Technologies De Lille-Lille 1 Procede de fabrication de vecteurs d'adn a partir de briques moleculaires contenant des sequences d'interet
JP7335224B2 (ja) * 2017-07-18 2023-08-29 ジェノヴィー エービー 全長ｔ細胞受容体オープンリーディングフレームの迅速な組立ておよび多様化のための二成分ベクターライブラリシステム
EP3987043A4 (en) * 2019-06-21 2023-07-19 Asklepios Biopharmaceutical, Inc. PRODUCTION OF VECTORS USING THE PHAGEN REPLICATION ORIGIN
WO2021055760A1 (en) * 2019-09-18 2021-03-25 Intergalactic Therapeutics, Inc. Synthetic dna vectors and methods of use

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Publication number Publication date
US20240409975A1 (en) 2024-12-12
CA3233230A1 (en) 2023-03-30
JP2024535917A (ja) 2024-10-02
WO2023049937A3 (en) 2023-08-10
MX2024003870A (es) 2024-09-11
IL311778A (en) 2024-05-01
CN118679248A (zh) 2024-09-20
AU2022349697A1 (en) 2024-05-16
WO2023049937A2 (en) 2023-03-30
EP4408994A4 (en) 2025-04-09
EP4408994A2 (en) 2024-08-07

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