CN118679248A - 环状dna载体的合成产生 - Google Patents

环状dna载体的合成产生 Download PDF

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Publication number
CN118679248A
CN118679248A CN202280078582.8A CN202280078582A CN118679248A CN 118679248 A CN118679248 A CN 118679248A CN 202280078582 A CN202280078582 A CN 202280078582A CN 118679248 A CN118679248 A CN 118679248A
Authority
CN
China
Prior art keywords
therapeutic
dna
linear
sequence
dna vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202280078582.8A
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English (en)
Chinese (zh)
Inventor
J·许
E·海格姆
J·劳拉
J·肯尼迪
A·马奎尔
M·巴赫沙耶什
E·多恩布什
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aldfron LLC
Original Assignee
Aldfron LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aldfron LLC filed Critical Aldfron LLC
Publication of CN118679248A publication Critical patent/CN118679248A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CN202280078582.8A 2021-09-27 2022-09-27 环状dna载体的合成产生 Pending CN118679248A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163248801P 2021-09-27 2021-09-27
US63/248,801 2021-09-27
PCT/US2022/077108 WO2023049937A2 (en) 2021-09-27 2022-09-27 Synthetic production of circular dna vectors

Publications (1)

Publication Number Publication Date
CN118679248A true CN118679248A (zh) 2024-09-20

Family

ID=85721330

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202280078582.8A Pending CN118679248A (zh) 2021-09-27 2022-09-27 环状dna载体的合成产生

Country Status (10)

Country Link
US (1) US20240409975A1 (enExample)
EP (1) EP4408994A4 (enExample)
JP (1) JP2024535917A (enExample)
KR (1) KR20240126447A (enExample)
CN (1) CN118679248A (enExample)
AU (1) AU2022349697A1 (enExample)
CA (1) CA3233230A1 (enExample)
IL (1) IL311778A (enExample)
MX (1) MX2024003870A (enExample)
WO (1) WO2023049937A2 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2026515898A (ja) * 2023-04-28 2026-05-19 ユニバーシティ オブ メリーランド、バルティモア カウンティ インビトロでスーパーコイル状の環状ｄｎａを作製する方法
WO2025007021A2 (en) * 2023-06-30 2025-01-02 Novel Biotechnology Inc. Cell-free replication of a nucleic acid construct
WO2025175112A1 (en) * 2024-02-16 2025-08-21 University Of Maryland, Baltimore County Method for generating circular dna using heteroduplex thermostable ligation assembly of precursors produced by rolling circle amplification

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE340264T1 (de) * 2003-06-10 2006-10-15 Mologen Ag Zirkuläres expressionskonstrukt für gentherapeutische anwendungen
WO2006063355A2 (en) * 2004-12-11 2006-06-15 Cytogenix , Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US8153598B2 (en) * 2005-10-19 2012-04-10 Intrexon Corporation PKD ligands and polynucleotides encoding PKD ligands
GB201321123D0 (en) * 2013-11-29 2014-01-15 Linea Ab Q Amplification of circular molecules
JP7058502B2 (ja) * 2014-04-11 2022-04-22 レッドボールト バイオサイエンシズ エルピー ゲノム適用および治療適用のための、核酸分子のクローン複製および増幅のためのシステムおよび方法
FR3032447B1 (fr) * 2015-02-10 2020-02-21 Universite Des Sciences Et Technologies De Lille-Lille 1 Procede de fabrication de vecteurs d'adn a partir de briques moleculaires contenant des sequences d'interet
JP7335224B2 (ja) * 2017-07-18 2023-08-29 ジェノヴィー エービー 全長ｔ細胞受容体オープンリーディングフレームの迅速な組立ておよび多様化のための二成分ベクターライブラリシステム
EP3987043A4 (en) * 2019-06-21 2023-07-19 Asklepios Biopharmaceutical, Inc. PRODUCTION OF VECTORS USING THE PHAGEN REPLICATION ORIGIN
WO2021055760A1 (en) * 2019-09-18 2021-03-25 Intergalactic Therapeutics, Inc. Synthetic dna vectors and methods of use

Also Published As

Publication number Publication date
US20240409975A1 (en) 2024-12-12
CA3233230A1 (en) 2023-03-30
JP2024535917A (ja) 2024-10-02
WO2023049937A3 (en) 2023-08-10
MX2024003870A (es) 2024-09-11
IL311778A (en) 2024-05-01
AU2022349697A1 (en) 2024-05-16
KR20240126447A (ko) 2024-08-20
WO2023049937A2 (en) 2023-03-30
EP4408994A4 (en) 2025-04-09
EP4408994A2 (en) 2024-08-07

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