KR20240123826A - 원형 rna를 사용한 세포 재프로그래밍을 위한 조성물 및 방법 - Google Patents

원형 rna를 사용한 세포 재프로그래밍을 위한 조성물 및 방법 Download PDF

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KR20240123826A
KR20240123826A KR1020247023505A KR20247023505A KR20240123826A KR 20240123826 A KR20240123826 A KR 20240123826A KR 1020247023505 A KR1020247023505 A KR 1020247023505A KR 20247023505 A KR20247023505 A KR 20247023505A KR 20240123826 A KR20240123826 A KR 20240123826A
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cell
cells
circular
reprogramming
rna
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멜리사 카펜터
산토시 나라얀
오스틴 티엘
인 미란다 양
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엘레바테바이오 테크놀로지스, 인코포레이티드
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KR1020247023505A 2021-12-20 2022-12-19 원형 rna를 사용한 세포 재프로그래밍을 위한 조성물 및 방법 Pending KR20240123826A (ko)

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US202163291645P 2021-12-20 2021-12-20
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PCT/US2022/081898 WO2023122517A2 (en) 2021-12-20 2022-12-19 Compositions and methods for cellular reprogramming using circular rna

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US (1) US20240327799A1 (https=)
EP (1) EP4453205A4 (https=)
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KR (1) KR20240123826A (https=)
CN (1) CN118574930A (https=)
AU (1) AU2022420484A1 (https=)
CA (1) CA3240645A1 (https=)
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WO2025106897A1 (en) * 2023-11-16 2025-05-22 Colossal Biosciences Inc. Methods and compositions for creating induced pluripotent stem cells
WO2025240429A1 (en) * 2024-05-13 2025-11-20 Colossal Biosciences Inc. Methods and compositions for creating elephant induced pluripotent stem cells
CN118638781B (zh) * 2024-05-27 2025-05-16 毕昇(北京)生物科技有限公司 使用环状RNA和microRNA进行细胞重编程的组合物和方法

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US8496941B2 (en) * 2009-06-03 2013-07-30 National Institute Of Advanced Industrial Science And Technology Vectors for generating pluripotent stem cells and methods of producing pluripotent stem cells using the same
US20120301438A1 (en) * 2009-09-14 2012-11-29 The Johns Hopkins University Reprogramming Blood Cells to Pluripotent and Multipotent Stem Cells
PL2852671T3 (pl) * 2012-05-21 2019-06-28 The Regents Of The University Of California Wytwarzanie ludzkich komórek ips z użyciem syntetycznego samoreplikującego się rna
BR112021023411A2 (pt) * 2019-05-22 2022-02-01 Massachusetts Inst Technology Composições e métodos de rna circular
CA3217001A1 (en) * 2021-04-27 2022-11-03 Matthew Angel Circular rna
US20230042860A1 (en) * 2021-07-15 2023-02-09 Turn Biotechnologies, Inc. Polycistronic expression vectors

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