KR20240123808A - 포유동물 세포에서 이종 폴리펩티드의 효율적인 발현 및 분비를 위한 신호 펩티드 스크리닝 방법 - Google Patents
포유동물 세포에서 이종 폴리펩티드의 효율적인 발현 및 분비를 위한 신호 펩티드 스크리닝 방법 Download PDFInfo
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Images
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
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- C—CHEMISTRY; METALLURGY
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- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
- C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
- C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
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| FI20216346 | 2021-12-23 | ||
| FI20216346 | 2021-12-23 | ||
| PCT/FI2022/050871 WO2023118670A1 (en) | 2021-12-23 | 2022-12-23 | Method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells |
Publications (1)
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| KR20240123808A true KR20240123808A (ko) | 2024-08-14 |
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| IL (1) | IL313740A (https=) |
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| US8673822B2 (en) * | 2004-01-17 | 2014-03-18 | Korea Research Institute Of Bioscience And Biotechnology | Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom |
| AT503474A1 (de) * | 2006-03-29 | 2007-10-15 | Univ Wien Bodenkultur | Peptidbibliothek |
| CN104011080B (zh) * | 2011-12-22 | 2017-10-20 | 弗·哈夫曼-拉罗切有限公司 | 用于真核细胞的全长抗体展示系统及其用途 |
| BR112018012180A2 (pt) | 2015-12-17 | 2018-12-04 | Psioxus Therapeutics Ltd | vírus de codificação de um anticorpo ou fragmento de complexo anti-tcr |
| US20220334124A1 (en) | 2019-09-03 | 2022-10-20 | Sung Sub Kim | Method for ultra-rapidly selecting signal peptide to which individual barcode system for increasing protein productivity is introduced |
| KR102406463B1 (ko) | 2019-09-03 | 2022-06-10 | 재단법인 오송첨단의료산업진흥재단 | 단백질 생산성 향상을 위한 개별 바코딩 시스템 도입한 시그널 펩타이드 초고속 선별방법 |
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| US20250059561A1 (en) | 2025-02-20 |
| IL313740A (en) | 2024-08-01 |
| CA3241498A1 (en) | 2023-06-29 |
| AU2022418344A1 (en) | 2024-08-01 |
| JP2024545273A (ja) | 2024-12-05 |
| WO2023118670A1 (en) | 2023-06-29 |
| CN118696055A (zh) | 2024-09-24 |
| EP4453019A1 (en) | 2024-10-30 |
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