KR20240118861A - 재조합 단백질의 발현을 증가시키는 유전 인자 - Google Patents

재조합 단백질의 발현을 증가시키는 유전 인자 Download PDF

Info

Publication number
KR20240118861A
KR20240118861A KR1020247023314A KR20247023314A KR20240118861A KR 20240118861 A KR20240118861 A KR 20240118861A KR 1020247023314 A KR1020247023314 A KR 1020247023314A KR 20247023314 A KR20247023314 A KR 20247023314A KR 20240118861 A KR20240118861 A KR 20240118861A
Authority
KR
South Korea
Prior art keywords
promoter element
nucleic acid
promoter
yeast cell
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247023314A
Other languages
English (en)
Korean (ko)
Inventor
비스바조이 로이 크아우트리
스베틀라나 발라츠카야
마틴 앤드류 호이트
Original Assignee
임파서블 푸즈 인크.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 임파서블 푸즈 인크. filed Critical 임파서블 푸즈 인크.
Publication of KR20240118861A publication Critical patent/KR20240118861A/ko
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/102—Plasmid DNA for yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
KR1020247023314A 2021-12-16 2022-12-15 재조합 단백질의 발현을 증가시키는 유전 인자 Pending KR20240118861A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163290166P 2021-12-16 2021-12-16
US63/290,166 2021-12-16
PCT/US2022/053003 WO2023114395A1 (en) 2021-12-16 2022-12-15 A genetic factor to increase expression of recombinant proteins

Publications (1)

Publication Number Publication Date
KR20240118861A true KR20240118861A (ko) 2024-08-05

Family

ID=86767454

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247023314A Pending KR20240118861A (ko) 2021-12-16 2022-12-15 재조합 단백질의 발현을 증가시키는 유전 인자

Country Status (8)

Country Link
US (1) US20230193338A1 (https=)
EP (1) EP4448719A4 (https=)
JP (1) JP2024546883A (https=)
KR (1) KR20240118861A (https=)
AU (1) AU2022415449A1 (https=)
CA (1) CA3238958A1 (https=)
MX (1) MX2024007198A (https=)
WO (1) WO2023114395A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3294762T (pt) 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
AU2020257270B2 (en) 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
CN116790395B (zh) * 2023-06-28 2024-07-19 江南大学 一种合成高活性血红素类蛋白毕赤酵母底盘菌株的构建及应用
WO2025247966A1 (en) * 2024-05-29 2025-12-04 Mybios Gmbh Process for protein production in methylotrophic yeast host cell.

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT3294762T (pt) * 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
AU2020257270B2 (en) * 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
EP3959311A1 (en) * 2019-04-25 2022-03-02 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
US20210062206A1 (en) * 2019-07-08 2021-03-04 The Regents Of The University Of California Synthetic transcription factors

Also Published As

Publication number Publication date
EP4448719A1 (en) 2024-10-23
AU2022415449A1 (en) 2024-05-23
CA3238958A1 (en) 2023-06-22
US20230193338A1 (en) 2023-06-22
EP4448719A4 (en) 2025-12-10
MX2024007198A (es) 2024-06-26
WO2023114395A1 (en) 2023-06-22
JP2024546883A (ja) 2024-12-26

Similar Documents

Publication Publication Date Title
US12116699B2 (en) Materials and methods for protein production
KR20240118861A (ko) 재조합 단백질의 발현을 증가시키는 유전 인자
CA2598514C (en) Mutant aox 1 promoters
KR101262682B1 (ko) 유전자 발현 기술
KR20220004090A (ko) 헴-함유 단백질의 생산을 위한 균주 및 방법
CA2295190C (en) Improved protein expression strains
US20130273606A1 (en) Secretion yield of a protein of interest by in vivo proteolytic processing of a multimeric precursor
US20190010194A1 (en) Methanol-utilizing yeast-derived novel protein and method for producing protein of interest using same
JP7239205B2 (ja) 新規宿主細胞及びそれを用いた目的タンパク質の製造方法
EP3778865A1 (en) Novel cell and method for producing protein of interest using same
CN118414435A (zh) 酵母细胞中改善的分泌蛋白产生
KR100977446B1 (ko) 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법
WO2018110616A1 (ja) 新規宿主細胞及びそれを用いた目的タンパク質の製造方法
CN120842369A (zh) 促进酵母分泌牛乳铁蛋白的融合蛋白表达系统

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000