KR20240107875A - Composition for reducing methane emission from ruminants containing Creatinolfosfate - Google Patents
Composition for reducing methane emission from ruminants containing Creatinolfosfate Download PDFInfo
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- KR20240107875A KR20240107875A KR1020220190921A KR20220190921A KR20240107875A KR 20240107875 A KR20240107875 A KR 20240107875A KR 1020220190921 A KR1020220190921 A KR 1020220190921A KR 20220190921 A KR20220190921 A KR 20220190921A KR 20240107875 A KR20240107875 A KR 20240107875A
- Authority
- KR
- South Korea
- Prior art keywords
- feed
- methane
- ruminants
- feed additive
- additive composition
- Prior art date
Links
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 title claims abstract description 154
- 229960002956 creatinolfosfate Drugs 0.000 title claims abstract description 50
- FOIPWTMKYXWFGC-UHFFFAOYSA-N creatinolfosfate Chemical compound NC(=N)N(C)CCOP(O)(O)=O FOIPWTMKYXWFGC-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 241000282849 Ruminantia Species 0.000 title claims abstract description 42
- 239000000203 mixture Substances 0.000 title claims abstract description 39
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- 238000000034 method Methods 0.000 claims abstract description 14
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- 239000007789 gas Substances 0.000 description 25
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 12
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/22—Methane [CH4], e.g. from rice paddies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S426/00—Food or edible material: processes, compositions, and products
- Y10S426/80—Geriatric
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
본 발명은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 사료 첨가제 조성물을 반추동물에게 급여함으로써 반추동물의 메탄 발생량을 효과적으로 감소시킬 수 있으며, 반추동물의 소화율, 에너지 효율 및 반추위 발효성상에는 영향을 미치지 않는 반추동물의 메탄 저감용 사료 첨가제 조성물 및 반추동물의 메탄 저감 방법에 관한 것이다. The present invention can effectively reduce the amount of methane produced by ruminants by feeding a feed additive composition containing creatinolfosfate to ruminants, and does not affect the digestibility, energy efficiency, and rumen fermentation characteristics of ruminants. It relates to a feed additive composition for reducing methane in animals and a method for reducing methane in ruminants.
Description
본 발명은 메탄 생성 저감용 조성물에 관한 것으로, 구체적으로는 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 반추동물의 메탄 생성 저감용 사료첨가제 조성물 및 이를 이용한 반추동물의 메탄 생성 억제 방법에 관한 것이다.The present invention relates to a composition for reducing methane production, and specifically to a feed additive composition for reducing methane production in ruminant animals containing creatinol phosphate (Creatinolfosfate), and a method for suppressing methane production in ruminant animals using the same.
지구온난화가스의 농도 증가로 인하여 지구온난화가 가속되어 기상이변, 작물의 생산량 및 식량부족 등의 사태가 발생하고 있다. 지구 온난화는 지구 표면의 평균온도가 상승하는 현상으로서, 여러 온실가스들이 대기로 들어가 잔류하면서 적외선 복사열을 흡수하거나 재방출하여 유발되는 것으로 알려져 있으며, 현재 IPCC(Intergovernmental Panel on Climatic Change)는 이산화탄소(CO2), 메탄(CH4), 아산화질소(N2O), 수소불화탄소(HFCs), 과불화탄소(PFCs) 및 육불화황(SF6)을 6대 온실가스로 선정하였다. 산업화 이후 화석연료의 과다 사용 등으로 인해 온실가스 농도의 증가로 인하여 지구의 기온이 지속적으로 상승하는 추세에 있다.Due to the increase in the concentration of global warming gases, global warming is accelerating, resulting in abnormal weather conditions, crop production and food shortages. Global warming is a phenomenon in which the average temperature of the Earth's surface increases. It is known to be caused by various greenhouse gases entering the atmosphere and remaining there, absorbing or re-emitting infrared radiation. Currently, the Intergovernmental Panel on Climatic Change (IPCC) estimates that carbon dioxide (CO2) ), methane (CH4), nitrous oxide (N2O), hydrofluorocarbons (HFCs), perfluorocarbons (PFCs), and sulfur hexafluoride (SF6) were selected as the six major greenhouse gases. After industrialization, the Earth's temperature continues to rise due to an increase in greenhouse gas concentration due to excessive use of fossil fuels.
이중 메탄(CH4)가스는 이산화탄소에 비해 생산되는 양은 적지만 지구온난화에 미치는 영향력이 21배 높기 때문에, 이산화탄소 다음으로 지구 온난화에 큰 영향을 미치는 원인으로 알려져 있다. 이러한 메탄가스는 연못, 습지, 논밭, 반추가축의 장내발효 및 분뇨 분해와 같은 미생물 발효에 의해 생성되거나 석탄, 석유, 천연가스, 배기가스 등 자연과 산업 활동에 의해서도 생성된다. 특히 메탄은 반추동물의 위에서 정상적인 소화과정(장내발효)중 발생되는데, 재 축산 분야에 의해 방출되는 메탄가스는 연간 최대 115 Tg으로 전체 메탄발생량의 13~19%를 차지하고 있다.Of these, methane (CH4) gas is produced in a small amount compared to carbon dioxide, but its impact on global warming is 21 times higher, so it is known to be the second largest cause of global warming after carbon dioxide. This methane gas is produced by microbial fermentation such as intestinal fermentation and decomposition of manure in ponds, wetlands, rice fields, and ruminant livestock, or is also produced by natural and industrial activities such as coal, oil, natural gas, and exhaust gas. In particular, methane is generated during the normal digestion process (intestinal fermentation) in the stomach of ruminants, and methane gas emitted by livestock farming accounts for up to 115 Tg per year, accounting for 13-19% of the total methane production.
반추동물은 식물계 식품을 반추위(rumen)로 공지된 동물의 첫번째 위내에서 초기에 연화시키고 이어서 되새김질 거리(cud)로 공지된 반(semi)-소화된 덩어리를 역류시키고 이를 다시 씹음으로써 소화시키는 우제류(Artiodactyla) 목의 포유동물이다. 식물 물질을 추가로 파쇄하고 소화를 자극하기 위해 되새김질 거리를 다시 씹는 과정은 "되새김질(ruminating)"이라고 지칭한다.Ruminants are artiodactyls (artiodactyls) that digest plant-based foods by initially softening them in the animal's first stomach, known as the rumen, and then regurgitating the semi-digested mass, known as the cud, and chewing it again. It is a mammal of the order Artiodactyla. The process of chewing the cud again to further break up the plant material and stimulate digestion is referred to as "ruminating."
소, 버팔로, 양, 및 염소를 포함하는 반추동물은 메탄 생성 발효가 발생하는 큰 전위를 가진다. 반추위 소화관은 네 개의 위 격실, 반추위, 벌집위, 주름위 및 중판위로 구성된다. 이들 중 가장 크고 가장 중요한 것은 반추위이다. 반추위는 발효 격실로서 기능한다. 그것은 식물 물질을 파괴하는 메탄 생성 고세균을 포함하는 높은 군집의 미생물을 포함한다. 언급된 미생물은 흔히 메탄 생성 미생물로 언급된다. 고세균 군집은 성장을 위한 에너지를 발생시키도록 수소 및 이산화탄소, 혐기성 미생물 발효의 생성물을 사용하여, 최종 생성물로서 메탄을 생성한다. 최종으로, 메탄은 트림을 통해 반추위로부터 배출된다.Ruminants, including cattle, buffalo, sheep, and goats, have a large potential for methanogenic fermentation to occur. The ruminal digestive tract consists of four gastric compartments, the rumen, the mesoplasty, the rumen stomach, and the mesoplasty. The largest and most important of these is the rumen. The rumen functions as a fermentation compartment. It contains a high community of microorganisms, including methanogenic archaea, which destroy plant material. The microorganisms mentioned are commonly referred to as methanogenic microorganisms. Archaeal communities use hydrogen and carbon dioxide, products of anaerobic microbial fermentation, to generate energy for growth, producing methane as the end product. Finally, methane is expelled from the rumen through belching.
메탄생성반응은 반추 위 발효(rumen fermentation) 과정 동안에 수소(H2) 처리(hydrogen disposal)의 주요 경로(main route)이다(Beauchemin et al, 2008) 반추 위(rumen) 환경(milieu)으로부터 H2 의 제거는 반추 위 발효의 효율적인 지속을 위해 필수적인 반면에, 메탄생성반응으로부터의 메탄은 지구 온난화(global warming)에 기여하는 중요한 온실가스(greenhouse gas)(Steinfeld et al, 2006) 뿐만 아니라 동물에 대한 식이 에너지의 손실(Johnson and Johnson, 1995)로서의 원인임을 보여준다. 상기와 같은 문제(subjects)로 인하여 반추 동물로부터의 메탄 생산을 완화시키기 위한 사료 첨가물에 대한 세계적인 연구(global search)로 이어지고 있다.Methanogenesis is the main route of hydrogen disposal during rumen fermentation (Beauchemin et al, 2008) and removal of H2 from the rumen milieu. While is essential for the efficient continuation of rumen fermentation, methane from methanogenic reactions is an important greenhouse gas contributing to global warming (Steinfeld et al, 2006) as well as dietary energy for animals. It is shown that this is the cause of loss (Johnson and Johnson, 1995). The above-mentioned subjects have led to a global search for feed additives to mitigate methane production from ruminants.
반추동물의 메탄 생성을 감소시키는 화합물에 관한 선행기술로는 한국등록특허 제 10-1808957호 "시니그린을 포함하는 반추동물의 메탄 생성 저감용 사료 첨가제 조성물"에는 시니그린을 포함하는 반추동물의 메탄 생성 저감용 사료 첨가제 조성물 및 이를 이용한 반추동물의 메탄 생성억제 방법이 개시되어 있고, 한국등록특허 제 10-1911465호 "반추동물의 반추위 내 메탄가스 저감을 위한 질산염 코팅 조성물 및 이의 제조방법"에는 대두단백질 분말 100 중량부, 질산염 300 내지 600 중량부, 오일류 200 내지 550 중량부, 안정제 80 내지 200 중량부 및 증류수 400 내지 600 중량부를 포함하는 반추동물의 반추위 내 메탄가스 생성을 억제하며 순수 질산염을 사용함으로써 발생되는 독성물질인 아질산염을 생성시키지 않는 질산염 코팅 조성물이 개시되어 있다.Prior art regarding compounds that reduce methane production in ruminant animals includes Korean Patent No. 10-1808957, “Feed additive composition for reducing methane production in ruminant animals containing cinigreen,” which describes methane in ruminant animals containing cinigreen. A feed additive composition for reducing methane production and a method for suppressing methane production in ruminants using the same are disclosed, and Korean Patent No. 10-1911465, “Nitrate coating composition for reducing methane gas in the rumen of ruminants and method for manufacturing the same,” discloses soybeans. It contains 100 parts by weight of protein powder, 300 to 600 parts by weight of nitrate, 200 to 550 parts by weight of oil, 80 to 200 parts by weight of stabilizer, and 400 to 600 parts by weight of distilled water. It suppresses the production of methane gas in the rumen of ruminants and uses pure nitrate. A nitrate coating composition that does not generate nitrite, a toxic substance generated by nitrite, is disclosed.
메탄 생성 저감을 위해 종래에는 할로겐 화합물이나 아이노포오계(ionophore) 항생제, 2-bromoethanesul- phonate, lumazine 등과 같은 화학물질을 사용하는 방법이 주로 사용되었다. 하지만, 현재까지 제시된 방법들은 메탄가스 발생의 저감 효과가 미미할 뿐만 아니라, 사료의 조성에 따라 메탄 억제 효과가 변하는 문제가 있었다. 또한, 장시간 처리하였을 때 반추 미생물이 그 물질에 대해 적응하거나 그 물질을 분해하여 그 효과가 지속적이지 못하는 문제점도 있었다.To reduce methane production, methods using chemicals such as halogen compounds, ionophore antibiotics, 2-bromoethane sulphonate, and lumazine were mainly used in the past. However, the methods presented to date not only have a minimal effect on reducing methane gas generation, but also have a problem in that the methane suppression effect varies depending on the composition of the feed. In addition, when treated for a long time, there was a problem that the effect was not sustainable because the ruminant microorganisms adapted to the material or decomposed the material.
따라서, 반추동물의 생산성을 증대시킴과 동시에 반추동물에서 메탄 발생을 감소시키는데 효과적인 물질의 개발이 시급한 실정이다.Therefore, there is an urgent need to develop substances effective in increasing the productivity of ruminants and at the same time reducing methane production in ruminants.
본 발명자들은 반추위내 영양소 소화율과 기타 반추위 발효특성에는 영향을 미치지 않으면서도 발효 가스 생성을 억제시키는 조성물을 개발하기 위해 예의 연구 노력한 결과, 크레아티놀포스페이트를 포함하는 사료첨가용 조성물을 급여하는 경우, 반추위 소화율에는 영향을 미치지 않으면서 반추위 발효 가스 생산을 절감시키는 효과가 있음을 확인함으로써 본 발명을 완성하였다.The present inventors have made extensive research efforts to develop a composition that suppresses fermentation gas production without affecting rumen nutrient digestibility and other rumen fermentation characteristics. As a result, when feeding a feed additive composition containing creatinol phosphate, rumen The present invention was completed by confirming that it has the effect of reducing rumen fermentation gas production without affecting digestibility.
본 발명의 목적은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 반추동물의 메탄 생성 저감용 사료첨가제 조성물 및 이를 이용한 반추동물의 메탄 생성 억제 방법을 제공하는 것이다.The purpose of the present invention is to provide a feed additive composition for reducing methane production in ruminant animals containing creatinol phosphate and a method for suppressing methane production in ruminant animals using the same.
상기 목적을 달성하기 위하여,In order to achieve the above purpose,
본 발명은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 반추동물의 메탄 생성 저감용 사료 첨가제 조성물을 제공한다.The present invention provides a feed additive composition for reducing methane production in ruminants containing creatinol phosphate (Creatinolfosfate).
또한, 본 발명은 상기 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 사료 첨가제 조성물을 배합한 반추동물의 메탄 생성 저감용 사료 조성물을 제공한다.Additionally, the present invention provides a feed composition for reducing methane production in ruminants containing the feed additive composition containing creatinol phosphate.
또한, 본 발명은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 사료 첨가제 조성물을 동물에게 급여하는 단계를 포함하는, 반추동물의 메탄 생성 억제 방법을 제공한다.Additionally, the present invention provides a method for inhibiting methane production in ruminants, comprising feeding a feed additive composition containing creatinol phosphate to the animal.
본 발명에 따른 사료 첨가제 조성물을 반추동물에게 급여하면 반추위 장내 발효에 의한 메탄 발생을 감소시킴으로써 지구 온난화의 원인이 되는 온실가스의 발생을 감소시킬 수 있는 효과가 있다. 또한, 본 발명의 사료 첨가제 조성물을 급여하면 가축의 메탄 발생으로 인한 에너지 손실을 줄이고 실질적으로 사용할 수 있는 에너지를 증가시켜 생산성에 도움을 줄 수 있다.Feeding the feed additive composition according to the present invention to ruminants has the effect of reducing the generation of greenhouse gases that cause global warming by reducing methane generation due to fermentation in the rumen. Additionally, feeding the feed additive composition of the present invention can help productivity by reducing energy loss due to methane production in livestock and increasing practically usable energy.
도 1은 본 발명의 크레아티놀포스페이트(Creatinolfosfate)의 pharmacophore를 나타낸 도이다.
도 2는 본 발명의 크레아티놀포스페이트(Creatinolfosfate)의 Fit score를 나타낸 도이다.
도 3은 본 발명의 크레아티놀포스페이트(Creatinolfosfate)의 bind 를 나타낸 도이다.Figure 1 is a diagram showing the pharmacophore of creatinol phosphate (Creatinolfosfate) of the present invention.
Figure 2 is a diagram showing the Fit score of creatinol phosphate (Creatinolfosfate) of the present invention.
Figure 3 shows the creatinol phosphate of the present invention. This is a diagram showing bind .
이하, 본 발명을 상세히 설명한다.Hereinafter, the present invention will be described in detail.
본 발명은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 반추동물의 메탄 생성 저감용 사료 첨가제 조성물을 제공한다.The present invention provides a feed additive composition for reducing methane production in ruminants containing creatinol phosphate (Creatinolfosfate).
본 발명의 크레아티놀포스페이트 (Creatinolfosfate)는 IUPAC으로 표기한다면 2-[carbamimidoyl(methyl)amino]ethyl dihydrogen phosphate이고, 심장 치료제의 유효성분이며, 류마티스 관절염, 성숙한 B-세포 림프종 및 퇴행성 관절 질환의 치료, 당뇨병성 망막병증의 치료를 위해 연구되는 물질이다.Creatinolfosfate of the present invention is 2-[carbamimidoyl(methyl)amino]ethyl dihydrogen phosphate when expressed in IUPAC, and is an active ingredient in heart treatment, treatment of rheumatoid arthritis, mature B-cell lymphoma and degenerative joint disease, This is a substance being studied for the treatment of diabetic retinopathy.
본 발명의 메탄저감 화합물 크레아티놀포스페이트(Creatinolfosfate)은 하기 화학식 1로 정의될 수 있다.Creatinol phosphate, a methane reduction compound of the present invention, can be defined by the following formula (1).
[화학식 1][Formula 1]
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 분자식은 C4H12N3O4P 이다.The molecular formula of creatinol phosphate, a methane reduction compound of the present invention, is C 4 H 12 N 3 O 4 P.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 CAS number는 6903-79-3이다.The CAS number of creatinol phosphate, a methane reducing compound of the present invention, is 6903-79-3.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 분자량(molecular weight)은 197.13이다.The molecular weight of creatinol phosphate, a methane reduction compound of the present invention, is 197.13.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 XLop3은 -2.5이다.XLop3 of the methane reducing compound creatinol phosphate of the present invention is -2.5.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 수소결합 주개는 4이다.The hydrogen bond donor of the methane reduction compound creatinol phosphate of the present invention is 4.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 수소결합 받개는 5이다.The hydrogen bond acceptor of the methane reduction compound creatinol phosphate of the present invention is 5.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 회전결합수는 5이다.The number of rotational bonds of the methane reduction compound creatinol phosphate of the present invention is 5.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 분자의 극성표면적은 120Å2 이다.The polar surface area of the molecule of creatinol phosphate, a methane reduction compound of the present invention, is 120 Å 2 .
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 Fit score는 2.76이다.The Fit score of the methane reducing compound creatinol phosphate of the present invention is 2.76.
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 △G 결합 에너지는 -88.07kcal/mol이다.The ΔG binding energy of creatinol phosphate, a methane reduction compound of the present invention, is -88.07 kcal/mol.
본 발명의 용어 "반추동물"은 척추동물문 포유강 우제목(偶蹄目, Artiodactyla-발굽이 2개인 초식동물)의 일부로 반추특성 즉, 되새김질(chewing the cud)을 할 수 있는 특수한 소화기관(위) 때문에 붙여진 이름이다. 반추류는 우제류(artiodactyls) 중에서도 가장 진화된 동물로 유제류(有蹄, ungulate) 중에서도 숫자도 가장 많고 전 세계적으로 가장 널리 퍼져있는 동물이다. 반추라는 매우 독특한 소화기관을 가지고 있기 때문에 열악한 식생조건에서도 살아남을 수 있어 크게 번성할 수 있었을 것으로 보고 있다. 모든 반추동물은 혹위·벌집위·겹주름위·주름위 등 4개의 방으로 분화되어 있으며 이 중 l위와 2위가 되새김에 관여하므로 반추위[반추위(反芻胃), ruminant stomach]라 한다. 사료를 섭취하고 반추하는데 하루 12시간 이상 시간을 보낸다The term "ruminant" in the present invention is a part of the vertebrate phylum Artiodactyla (herbivores with two hooves) and has ruminating characteristics, that is, a special digestive organ (stomach) capable of chewing the cud. That's why it was given the name. Ruminants are the most evolved animals among artiodactyls, the most numerous among ungulates, and the most widespread worldwide. Because rumination has a very unique digestive system, it is believed that it was able to survive even in poor vegetation conditions and thus thrive. All ruminants are divided into four chambers, including the hump stomach, honeycomb stomach, fold stomach, and fold stomach. Among these, the first and second stomachs are involved in rumination, so they are called the ruminant stomach. Spend more than 12 hours a day eating and ruminating
반추동물의 위는 보통 4개의 위로 구성되며, 반추위 내에는 여러 종류의 미생물이 살고 있으며 이 미생물들이 섬유질이 많은 풀사료들을 효과적으로 분해, 발효, 소화 시켜 필요한 영양분을 동물에게 공급한다.The stomach of a ruminant animal usually consists of four stomachs, and various types of microorganisms live within the rumen. These microorganisms effectively decompose, ferment, and digest high-fiber grass feed to supply necessary nutrients to the animal.
제1위(혹위, rumen) : 4개의 위 중 가장 크다(소의 경우 150∼200ℓ). 섭취된 모든 사료는 먼저 이곳에 모이고 거칠기, 크기, 비중, 형태에 따라 바로 2~4위로 보낸다. 2위를 거쳐 되돌아온 거친 사료는 뭉쳐진 다음 토출되어 40∼60회 정도 씹은 다음 다시 삼키는 되새김이 이뤄어 지며, 씹어서 되돌아 온 첫째 위의 내용물은 바로 둘째 위로 간다. 소는 40∼50분 동안 이 동작을 반복하고 완전히 소화될 때까지 하루 종일 몇 번이고 되풀이한다. 첫째위 속에는 많은 세균이 있어서 셀룰로오스를 분해시킨다. 발효산물 중 이산화탄소와 메탄가스는 호기에 의해 체외로 배출되며 휘발성지방산(VFA)과 암모니아는 위벽의 유두(papillae)에 의해 혈관으로 흡수된다. 여기서 흡수된 암모니아는 간장에서 요소로 변화된 후 타액과 전위벽을 통해 위내로 순환, 유입되어 미생물 증식에 사용된다.1st stomach (rumen): The largest of the four stomachs (150-200 liters in cows). All ingested feed is first collected here and sent directly to the 2nd to 4th chambers depending on roughness, size, specific gravity, and shape. The rough feed returned through the second stomach is lumped together and then expelled, chewed 40 to 60 times, and then swallowed again. The contents of the first stomach returned after chewing go directly to the second stomach. The cow repeats this action for 40 to 50 minutes and several times throughout the day until it is completely digested. First, there are many bacteria in the stomach that break down cellulose. Among fermentation products, carbon dioxide and methane gas are discharged out of the body through exhalation, and volatile fatty acids (VFA) and ammonia are absorbed into the blood vessels through papillae of the stomach wall. Here, the absorbed ammonia is converted into urea in the liver and then circulates and flows into the stomach through saliva and the gastric wall, where it is used to propagate microorganisms.
제2위(벌집위, reticulum) : 내벽이 벌집같이 구획되어 벌집위(아래 그림 참조)라고도 한다. 첫째 위로부터 들어온 사료 중 일정한 크기 이상의 사료는 수축이완 운동을 통하여 다시 첫째 위로 보내고 일정한 크기 이하의 사료는 계속해서 발효된 다음 제3위로 보내진다.2nd stomach (reticulum): The inner wall is divided like a honeycomb, so it is also called a honeycomb stomach (see picture below). Among the feeds that come in from the first stomach, those of a certain size or larger are sent back to the first stomach through contraction and relaxation movements, and those of a certain size or less are continuously fermented and then sent to the third stomach.
제3위 - 겹주름위(omasum, 重瓣胃) : 점막 근육이 수많은 주름으로 되어 있어 겹주름위, 중판위라는 이름이 붙었다. 제2위로부터 넘어온 소화물은 4위로 들어가기 전에 이곳에서는 소화물 입자 크기가 감소되고 여분의 물이 흡수된다.3rd stomach - fold fold stomach (omasum, heavy stomach): The mucosal muscles are made up of numerous wrinkles, so they are named fold fold stomach and middle plate stomach. Before the digested matter from the second stomach enters the fourth stomach, the particle size of the digested matter is reduced and excess water is absorbed.
제4위 - 주름위(abomasum, 皺胃) : 제3위에서 가늘게 부서진 사료와 미생물의 일부는 제4위로 이동하여 위선으로부터 분비되는 위액에 의해 소화된다. 제4위는 단위 동물의 위와 같은 기능을 하므로 진위라고도 하며 위산, 펩신, 레닌 등의 소화 효소가 분비된다.4th stomach - Abomasum: Some of the finely broken feed and microorganisms in the 3rd stomach move to the 4th stomach and are digested by gastric juice secreted from the stomach glands. The fourth stomach is also called the true stomach because it has the same function as the stomach of monogastric animals. It secretes digestive enzymes such as gastric acid, pepsin, and renin.
본 발명의 반추동물은 소, 염소, 황소, 물소, 들소, 사슴, 말코손바닥사슴, 낙타 또는 양일 수 있으나, 이에 제한되지 않는다.The ruminant animal of the present invention may be a cow, goat, bull, buffalo, bison, deer, moose, camel, or sheep, but is not limited thereto.
본 발명의 반추동물은 한우일 수 있다.The ruminant animal of the present invention may be Korean beef.
본 발명의 용어 "한우"는 종래부터 한반도에서 운반용이나 농경용으로 사육해오던 재래종의 소를 의미한다.The term "Korean beef" in the present invention refers to a native breed of cattle that has been traditionally raised on the Korean Peninsula for transportation or agricultural purposes.
본 발명의 용어 "사료 첨가제"는 생산성 개선이나 성장 촉진, 영양소 보충 및 체중감소 예방, 사료내 섬유소의 소화 이용성 증진, 유질 개선, 번식장애 예방 및 수태율 향상, 하절기 고온 스트레스 예방 등 다양한 효과를 목적으로 사료에 소량 배합하는 비 영양소 보조물질로 정의할 수 있으며, 아미노산제, 비타민제, 항생제, 생균제, 효소제, 유기산제, 향미제, 감미제, 항산화제, 각종 천연물질 및 기능성 물질 등이 사료첨가제로 분류될 수 있다. The term "feed additive" of the present invention is used for various effects such as improving productivity or promoting growth, supplementing nutrients and preventing weight loss, improving digestibility of fiber in feed, improving oil quality, preventing reproductive disorders and improving conception rate, and preventing high temperature stress in the summer. It can be defined as a non-nutrient auxiliary material mixed in small amounts in feed. Amino acids, vitamins, antibiotics, probiotics, enzymes, organic acids, flavoring agents, sweeteners, antioxidants, various natural substances and functional substances can be classified as feed additives. You can.
본 발명의 사료 첨가제 조성물은 크레아티놀포스페이트을 50 내지 150 ppm의 농도로 포함할 수 있고, 바람직하게는 60 내지 120 ppm의 농도로 포함할 수 있다.The feed additive composition of the present invention may contain creatinol phosphate at a concentration of 50 to 150 ppm, preferably at a concentration of 60 to 120 ppm.
본 발명의 사료 첨가제 조성물은 아황산나트륨, 파라옥시벤조산메틸, 파라옥시벤조산프로필, 프로필렌글리콜, 폴리에틸렌글리콜, 에탄올, 폴리소르베이트, 피마자유, 벤질알콜, 트리에탄올아민, 글리세린, 젖산, 포비돈, 디메틸설폭시드, N-메틸피롤리돈 및 소디움벤조에이트로 이루어진 군에서 선택되는 1종 이상의 부형제를 더 포함할 수 있다.The feed additive composition of the present invention includes sodium sulfite, methyl paraoxybenzoate, propyl paraoxybenzoate, propylene glycol, polyethylene glycol, ethanol, polysorbate, castor oil, benzyl alcohol, triethanolamine, glycerin, lactic acid, povidone, and dimethyl sulfoxide. , it may further include one or more excipients selected from the group consisting of N-methylpyrrolidone and sodium benzoate.
본 발명의 사료 첨가제 조성물은 정제수를 더 포함할 수 있다.The feed additive composition of the present invention may further include purified water.
본 발명의 사료 첨가제 조성물은 액제 및 정제로 이루어진 군에서 선택되는 제형일 수 있으나, 이에 한정되는 것은 아니다.The feed additive composition of the present invention may be a formulation selected from the group consisting of liquid and tablets, but is not limited thereto.
본 발명의 사료 첨가제는 당업계에 공지된 다양한 사료 제조방법에 따라 적절한 유효 농도 범위에서 유기태 구리를 첨가하여 제조 가능하다.The feed additive of the present invention can be manufactured by adding organic copper in an appropriate effective concentration range according to various feed manufacturing methods known in the art.
본 발명의 실시예에서, 크레아티놀포스페이트은 건물 소화율에 영향을 주지 않으면서 메탄 가스 생성을 저해한다.In embodiments of the invention, creatinol phosphate inhibits methane gas production without affecting dry matter digestibility.
본 발명에서 총 가스량(mL)은 사료 소화 중 발생하는 총 가스량을 나타낸다. In the present invention, the total gas amount (mL) represents the total amount of gas generated during feed digestion.
본 발명에서 메탄 발생량의 측정에 있어서 총 가스량 중 메탄(%)은 한우에서 발생한 총 가스량 중 메탄의 함량을 나타내며, mL은 상기 메탄함량을 mL단위로 환산한 것이다. 또한, mL/건물량(g)은 한우에서 발생한 메탄(mL)을 사료(건물기준) 당 발생량으로 환산한 것이고, mL/소화 건물량(g)은 한우에서 발생한 메탄(mL)을 사료(건물기준) 소화 당 발생량으로 환산한 것이다. In the present invention, in measuring the amount of methane generated, methane (%) of the total gas amount represents the content of methane in the total amount of gas generated from Korean beef, and mL is the methane content converted to mL. In addition, mL/dry matter (g) is the conversion of methane (mL) generated from Korean beef into the amount generated per feed (dry matter basis), and mL/digested dry matter (g) is the methane (mL) generated from Korean beef converted to the amount generated per feed (dry matter basis). Standard) Converted to the amount generated per digested sugar.
본 발명에서 건물소화율(%)은 한우의 반추위내 사료(건물기준) 소화율을 나타낸다. In the present invention, dry matter digestibility (%) represents the digestibility of feed (dry matter basis) in the rumen of Korean cattle.
또한, 본 발명은 상기 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 사료 첨가제 조성물을 배합한 반추동물의 메탄 생성 저감용 사료 조성물을 제공한다.Additionally, the present invention provides a feed composition for reducing methane production in ruminants containing the feed additive composition containing creatinol phosphate.
본 발명의 용어 "사료"는 가축이나 동물, 어류 등에 영양이 되거나 건강 유지 또는 성장에 필요한 것으로 단미사료, 배합사료, 보조사료, 농후사료 등을 의미한다. The term "feed" in the present invention refers to something that is nutritious to livestock, animals, fish, etc. or necessary for health maintenance or growth, such as sweet feed, compound feed, supplementary feed, and concentrated feed.
상기 "배합사료"란 단미사료 보조사료 등을 적정 비율로 배합 또는 가공한 것을 의미한다.The term “compounded feed” means a product in which single feed, supplementary feed, etc. are mixed or processed in an appropriate ratio.
상기 "단미사료"는 식물성 동물성 또는 광물성 물질로서 사료로 직접 사용되거나 배합사료의 원료로 사용되는 것을 말한다.The above “single feed” refers to a vegetable, animal or mineral substance that is used directly as feed or as a raw material for compound feed.
상기 "보조사료"는 사료의 품질저하 방지 또는 사료의 효용을 높이기 위하여 사료에 첨가하는 것을 말한다.The above “supplementary feed” refers to what is added to feed to prevent deterioration of feed quality or increase the utility of feed.
본 발명의 배합 사료는 옥수수, 대두 껍질, 밀기울, 옥수수 글루텐 피드, 대두박, 루핀 씨앗, 비타민, 소금, 석회암, 탄산수소나트륨, 및 볏짚 사일리지를 포함할 수 있지만, 이에 제한되지 않는다.The combined feed of the present invention may include, but is not limited to, corn, soybean hulls, wheat bran, corn gluten feed, soybean meal, lupine seeds, vitamins, salt, limestone, sodium bicarbonate, and rice straw silage.
또한, 본 발명은 크레아티놀포스페이트(Creatinolfosfate)를 포함하는 사료 첨가제 조성물을 동물에게 급여하는 단계를 포함하는, 반추동물의 메탄 생성 억제 방법을 제공한다.Additionally, the present invention provides a method for inhibiting methane production in ruminants, comprising feeding a feed additive composition containing creatinol phosphate to the animal.
상기 크레아티놀포스페이트은 50 내지 150 ppm의 농도로 급여될 수 있고, 바람직하게는 60 내지 120 ppm의 농도로 급여될 수 있다.The creatinol phosphate can be fed at a concentration of 50 to 150 ppm, preferably at a concentration of 60 to 120 ppm.
상기 소는 한우일 수 있지만 이에 제한되지 않는다.The cow may be Korean beef, but is not limited thereto.
구체적인 실시예에서, 본 발명자들은 크레아티놀포스페이트를 60 또는 120 ppm의 농도로 포함하는 사료 첨가제를 첨가하여 in vitro 분석에서 반추위액을 24시간 배양하고, 반추위액 배양 내 총 가스와 총 가스량 중 메탄 발생량, 및 건물소화율의 영향을 측정하였고, 그 결과 60 또는 120 ppm의 크레아티놀포스페이트 처리구에서 대조구 대비 건물 소화율 저감 없이 메탄 가스 발생량이 감소함을 확인함으로써 본 발명을 완성하였다.In a specific example, the present inventors cultured rumen fluid for 24 hours in an in vitro analysis by adding a feed additive containing creatinol phosphate at a concentration of 60 or 120 ppm, and measured the total gas in the rumen fluid culture and the amount of methane generated in the total amount of gas. , and the effect of dry matter digestibility was measured, and as a result, the present invention was completed by confirming that the amount of methane gas generated was reduced in the 60 or 120 ppm creatinol phosphate treatment group without reducing dry matter digestibility compared to the control group.
이하, 본 발명을 실시예에 의하여 상세히 설명한다. Hereinafter, the present invention will be described in detail through examples.
단, 하기 실시예는 본 발명을 예시하는 것이며, 본 발명의 권리 범위는 이에 한정되는 것은 아니고 청구 범위에서 정의하고 있는 본 발명의 기본 개념을 이용한 당업자의 여러 변형 및 개량 형태 또한 본 발명의 권리 범위에 속하는 것이다.However, the following examples illustrate the present invention, and the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the claims are also included in the scope of the present invention. belongs to
<실시예 1> <Example 1>
기존에 메탄저감 효과를 보인 기 보고된 3-NOP(3-Nitrooxypropanol, C3H7NO4)와 유사한 화합물을 탐색하여, 메탄생성 과정을 방해하는 화합물 후보로, By searching for compounds similar to 3-NOP (3-Nitrooxypropanol, C 3 H 7 NO 4 ), which has previously been reported to have a methane reduction effect, we searched for compounds that interfere with the methane production process.
분자식은 C4H12N3O4P이고, 분자량(molecular weight)은 197.13이며, CAS number는 6903-79-3이고, XLop3은 -2.5이고, 수소결합 주개는 4, 수소결합 받개는 5, 회전결합수는 5, 분자의 극성표면적은 120Å2 인 크레아티놀포스페이트(Creatinolfosfate)를 발굴하였다(도 1).The molecular formula is C 4 H 12 N 3 O 4 P, the molecular weight is 197.13, the CAS number is 6903-79-3, XLop3 is -2.5, the hydrogen bond donor is 4, the hydrogen bond acceptor is 5, Creatinol phosphate was discovered with a rotational bond number of 5 and a molecular polar surface area of 120Å 2 (Figure 1).
본 발명의 메탄저감 화합물 크레아티놀포스페이트의 Fit score, 및 △G 결합 에너지는 도 2 및 3에 나타내었다.The fit score and ΔG binding energy of the methane reducing compound creatinol phosphate of the present invention are shown in Figures 2 and 3.
<실험예 1> 반추위액 채취 및 시료배양<Experimental Example 1> Ruminal fluid collection and sample culture
캐뉼라(cannula)가 장착된 한우 3두 (볏짚·농후사료 비율 = 2:8, 생체중 736 kg)에서 반추위액을 거즈 8겹으로 사료입자 제거 후 채취하였다. 상기 채취한 반추위액은 멸균한 McDougall buffer(표 1)를 1:4 비율로 혼합한 후 39℃로 교반 및 혐기적 상태를 유지하고, in vitro 실험용 serum bottle에 0.5 g의 기질(볏짚·농후사료 비율 = 2:8)과 실시예 1의 크레아티놀포스페이트 (60 ppm 또는 120 ppm)가 포함된 filter bag을 넣고 buffer와 혼합된 반추위액(배양액)을 50 mL 분주 후 24시간 배양하였다. Rumen fluid was collected from 3 Korean cattle (rice straw/concentrated feed ratio = 2:8, live weight 736 kg) equipped with a cannula after removing feed particles with 8 layers of gauze. The collected rumen fluid was mixed with sterilized McDougall buffer (Table 1) at a ratio of 1:4, stirred at 39°C and maintained in an anaerobic state, and 0.5 g of substrate (rice straw/concentrated feed) was added to a serum bottle for in vitro experiments. Ratio = 2:8) and a filter bag containing creatinol phosphate (60 ppm or 120 ppm) of Example 1 was placed, 50 mL of rumen fluid (culture fluid) mixed with buffer was dispensed, and cultured for 24 hours.
McDougall buffer 혼합 비율(McDougall, 1948)McDougall buffer mixing ratio (McDougall, 1948)
상기 배양 후, 가스 발생량을 측정하고 filter bag을 꺼내 소화율 분석에 이용하고 배양액은 발효성상 분석에 이용하였다. 실험에 사용된 조사료는 볏짚이고, 농후사료는 제조하여 사용하였다. 배합사료 배합비는 하기 표 2에 나타내었고, 농후사료, 볏짚 영양성분은 표 3에 나타내었다.After the culture, the amount of gas generated was measured, the filter bag was taken out and used for digestibility analysis, and the culture medium was used for fermentation characteristics analysis. The roughage used in the experiment was rice straw, and the concentrated feed was manufactured and used. The mixing ratio of the mixed feed is shown in Table 2 below, and the nutritional components of the concentrated feed and rice straw are shown in Table 3.
<실험예 2> 반추위 가스발생 분석<Experimental Example 2> Rumen gas generation analysis
배양이 종료된 serum bottle에 50 mL 주사기(H13.SYS050,DH, South Korea)를 이용하여 가스발생량(mL)을 측정하였다. 상기 주사기에 포집된 가스는 진공튜브(BD 367953, 8.5 mL)에 옮겨 담아 GC (gas chromatography, NL/450 GC, Bruker, USA)로 분석하였다. Gas-tight syringe를 이용하여 GC에 gas를 1.0 mL씩 주입하였으며 22.4 μM, 63.5 μM, 242.2 μM의 메탄을 표준가스로 사용하였다.The amount of gas generated (mL) was measured using a 50 mL syringe (H13.SYS050, DH, South Korea) in the serum bottle where the culture was completed. The gas collected in the syringe was transferred to a vacuum tube (BD 367953, 8.5 mL) and analyzed by GC (gas chromatography, NL/450 GC, Bruker, USA). 1.0 mL of gas was injected into the GC using a gas-tight syringe, and 22.4 μM, 63.5 μM, and 242.2 μM methane were used as standard gases.
메탄 발생량 분석을 위한 GC의 분석 조건은 오븐 온도는 90℃, injecter 온도는 260℃, split ratio는 10, total flow는 55.8ml/min, column flow는 5ml/min으로 설정하고 이동상으로 헬륨을 사용하였으며, 칼럼은 GS-GasPro 113-4332(Agilent, 30 m×0.32 mm, -80~260℃)를 이용하여 분석하였다. The GC analysis conditions for methane production analysis were oven temperature at 90℃, injector temperature at 260℃, split ratio at 10, total flow at 55.8ml/min, column flow at 5ml/min, and helium was used as the mobile phase. , the column was analyzed using GS-GasPro 113-4332 (Agilent, 30 m×0.32 mm, -80~260°C).
실험 결과, 실시예 1의 크레아티놀포스페이트 (60ppm 및 120ppm) 24시간 in vitro 배양 시, 메탄 발생량(mL/소화 건물량)이 유의적으로 감소하였다(표 4). As a result of the experiment, when creatinol phosphate (60ppm and 120ppm) of Example 1 was cultured in vitro for 24 hours, the amount of methane generated (mL/digested dry matter) was significantly reduced (Table 4).
상기 결과를 통해 크레아티놀포스페이트을 메탄발생량 감소 후보 물질(약용작물)로 판단할 수 있었다.Through the above results, creatinol phosphate could be determined as a candidate substance (medicinal crop) for reducing methane production.
<실험예 3> 건물 소화율 분석<Experimental Example 3> Dry matter digestibility analysis
Serum bottle에서 꺼낸 filter bag은 불순물이 제거될 때까지 세척하고, 세척이 끝난 filter bag은 60℃의 건조오븐에서 24시간 건조 후 무게를 측정하였다. 이 때, 건물 소화율은 기존 샘플 무게와 배양 후 건조 무게를 비교하여 산정하였다. The filter bag removed from the serum bottle was washed until impurities were removed, and the washed filter bag was dried in a drying oven at 60°C for 24 hours and then weighed. At this time, dry matter digestibility was calculated by comparing the existing sample weight and the dry weight after culture.
실험 결과, 건물 소화율은 실시예 1의 크레아티놀포스페이트 (60ppm 및 120ppm) 처리구와 대조구를 비교하였을 때 유의적인 차이가 없었다.As a result of the experiment, there was no significant difference in dry matter digestibility when comparing the creatinol phosphate (60 ppm and 120 ppm) treatment group of Example 1 and the control group.
상기 결과는 크레아티놀포스페이트가 건물소화율에는 부정적인 영향이 없으면서 반추위 발효에 따른 메탄 발생량을 감소시키는 효과가 있음을 나타낸다. The above results indicate that creatinol phosphate has the effect of reducing methane production due to rumen fermentation without having a negative effect on dry matter digestibility.
(건물소화량 기준, %)Methane production reduction rate 1)
(Based on building fire extinguishment volume, %)
감소율 유의성Methane generation
Decline rate significance
메탄 발생 감소율=(대조구-처리구)/대조구*100, Methane generation reduction rate = (control - treatment) / control * 100,
건물 소화율 감소=(대조구-처리구)/대조구*100Reduction in dry matter digestibility = (control group - treatment group) / control group * 100
(-): 메탄발생량·건물소화율 증가(-): Increased methane generation and building digestibility
Claims (11)
A feed additive composition for reducing methane production in ruminants containing creatinol phosphate.
상기 크레아티놀포스페이트는 하기 화학식 1로 정의되는 것을 특징으로 하는, 사료 첨가제 조성물.
[화학식 1]
According to paragraph 1,
The creatinol phosphate is a feed additive composition, characterized in that defined by the following formula (1).
[Formula 1]
상기 반추동물은 소, 염소, 황소, 물소, 들소, 사슴, 말코손바닥사슴, 낙타 또는 양인 것을 특징으로 하는, 사료 첨가제 조성물.
According to paragraph 1,
A feed additive composition, wherein the ruminant animal is a cow, goat, bull, water buffalo, bison, deer, moose, camel or sheep.
상기 반추동물은 한우인 것을 특징으로 하는, 사료 첨가제 조성물.
According to paragraph 3,
A feed additive composition, characterized in that the ruminant animal is Korean beef.
상기 크레아티놀포스페이트은 50 내지 150 ppm의 농도로 첨가되는 것을 특징으로 하는, 사료 첨가제 조성물.
According to paragraph 1,
The feed additive composition, characterized in that the creatinol phosphate is added at a concentration of 50 to 150 ppm.
상기 조성물은 반추동물의 건물 소화율에 영향을 주지 않는 것을 특징으로 하는, 사료 첨가제 조성물.
According to paragraph 1,
A feed additive composition, characterized in that the composition does not affect the dry matter digestibility of ruminants.
A feed composition for reducing methane production in ruminants containing the feed additive composition of claim 1.
상기 사료 조성물은 건물, 조단백, 조지방, 비섬유성탄수화물, 중성지방불용섬유소, 산성지방불용섬유소 및 조회분을 더 포함하는 것을 특징으로 하는, 사료 조성물.
In clause 7,
The feed composition further comprises dry matter, crude protein, crude fat, non-fiber carbohydrates, neutral fat-insoluble fiber, acidic fat-insoluble fiber, and ash.
A method for inhibiting methane production in ruminants, comprising feeding the feed additive composition containing the creatinol phosphate of claim 1 to the animal.
상기 크레아티놀포스페이트은 50 내지 150 ppm의 농도로 급여되는 것을 특징으로 하는, 방법.
According to clause 9,
The method is characterized in that the creatinol phosphate is fed at a concentration of 50 to 150 ppm.
상기 소는 한우인 것을 특징으로 하는, 방법.
According to clause 9,
A method, characterized in that the cow is Korean beef.
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