KR20240086953A - Composition for improving blood vessel health, blood flow, sexual function, exercise ablility or anti-fatigue comprising panaxana ginsan, ginseng extract obtained by extracting/purifying ginseng comprising ginseng saponin as standard compound together, and rhodiola rosea, elm root, butterbur, saururus chinensis - Google Patents

Abstract

The present invention uses as indicator ingredients the active ingredients of Korean ginseng, especially ginseng saponin, which is effective in improving immunity, fatigue recovery, and improving blood circulation, and ginseng polysaccharide, which has anti-cancer immunity regulation, hematopoiesis promotion, and radiation protection effects. It relates to a composition for improving vascular health, improving blood circulation, improving sexual function, improving exercise ability, or relieving fatigue, including ginseng extract Panaxena ginsangwa, rhodiola extract, Yu root bark extract, and butterbur and trillium extract complex extract. The composition, which is one aspect of the present invention, has excellent effects on improving blood vessel health and blood circulation. The composition can be expected to improve sexual function, exercise function, and fatigue recovery by improving vascular health and blood circulation, as well as improving overall physical function. In addition, the composition according to the present invention is harmless to the body and has no side effects, so it can be taken for a long period of time.

Description

Ginseng saponin derived from Korean ginseng, which has the effect of enhancing immunity, recovering from fatigue and improving blood circulation, and ginseng polysaccharide, which has the effect of regulating anti-cancer immunity, promoting hematopoiesis and protecting against radiation, together with panaxena ginsan and Rhodiola rosea, which are ginseng extracts obtained by extracting and purifying them as indicator ingredients. A composition for improving vascular health, blood circulation, sexual function, exercise ability, or relieving fatigue, containing extracts of radicle root bark, butterbur, and trifolium prickly pear as active ingredients GINSAN, GINSENG EXTRACT OBTAINED BY EXTRACTING/PURIFYING GINSENG COMPRISING GINSENG SAPONIN AS STANDARD COMPOUND TOGETHER, AND RHODIOLA ROSEA, ELM ROOT, BUTTERBUR, SAURURUS CHINENSIS}

The present invention uses as indicator ingredients the active ingredients of Korean ginseng, especially ginseng saponin, which is effective in enhancing immunity, recovering from fatigue, and improving blood circulation, and ginseng polysaccharide, which has anti-cancer, immune-boosting, hematopoiesis-promoting, and radiation-protective effects. It relates to a composition for improving vascular health, improving blood circulation, improving sexual function, improving exercise ability, or relieving fatigue, containing a high content of ginseng extract, rhodiola extract, rhodiola root extract, butterbur extract, and cypress extract.

Currently living in a super-aging society, the incidence of patients suffering from geriatric diseases that occur after menopause such as dementia, hypertension, diabetes, stroke, myocardial infarction, cardiac arrhythmia, and various types of cancer is increasing exponentially. Cardiovascular diseases caused by blood circulation disorders such as arteriosclerosis and stroke are a major cause of death in aging societies around the world, including in the United States, Europe, and Asia. As Korea also entered an aging society at the end of the 20th century, it was found to be the first or second cause of mortality.

Blood is composed of plasma and blood cells and plays an important role in maintaining homeostasis in each tissue by supplying oxygen and nutrients to each tissue of the body and removing waste products generated by intracellular metabolism. In order to perform this role, the functions of constituent cells and tissues must be maintained normally, and blood circulation must also be maintained smoothly. If blood circulation (blood circulation) is not smooth, the speed of fatigue recovery slows down due to waste accumulated in each part of the body, causing easy fatigue or decreased exercise ability. In men, erectile dysfunction and They also suffer from the same sexual dysfunction. In addition, if blood circulation decreases, cardiovascular diseases that directly affect life, such as arteriosclerosis and stroke, may develop.

Plasma is composed of coagulation factors related to blood coagulation, exchanges various interactions with blood cells such as platelets, red blood cells, and immune cells, and maintains the equilibrium state of each cell function. Among these, platelets are small cells with a diameter of 2 to 4㎛ that are released into the blood when the cytoplasm of megakaryocytes, large cells of bone marrow, are shed and are cells with a short life span of about 5 to 7 days. Platelets are cells that are activated when blood vessels are damaged and are responsible for hemostasis by aggregating at the damaged area. When blood vessels are damaged, collagen, von Willebrand factor, and fibronectin in the inner walls of blood vessels are exposed and promote platelet adhesion. As platelets are activated, they release serotonin, Ca2+, thromboxane A2, etc. to amplify the aggregation of other surrounding platelets and react with coagulation factors present in plasma to form clots. Allows hemostasis to occur more quickly and effectively. Hemostasis can be said to be a defense mechanism to minimize blood loss from damaged areas and maintain normal circulation of blood. In normal blood vessels, homeostasis is maintained by balancing the activation reaction of the hemostatic mechanism with the inhibitory reaction and clot decomposition reaction. However, excessive hemostasis and the formation of blood clots impede blood flow, cause blood circulation abnormalities, and cause lesions such as thrombuses. When a blood clot is formed, it causes blood circulation problems in the veins, causing edema or inflammation, and causes ischemia or infarction in the arteries, resulting in diseases such as myocardial infarction, stroke, and pulmonary artery infarction. In addition, when platelets are activated, various vascular regulatory factors (serotonin, thromboxaneA2, PAF [platelet activating factor], etc.) are released and can constrict blood vessels. When blood vessels constrict, blood flow is blocked, blood flow speed changes, platelet activation is amplified, and vascular endothelial damage progresses, impeding normal cardiovascular function. It is known that such blood circulation abnormalities cause the development of vascular diseases such as arteriosclerosis, myocardial infarction, cardiac ischemic disease, and cerebrovascular disease. Vascular endothelial cells, which make up blood vessels, exist as a single layer on the intima surface of the blood vessel layer and serve as a diffusion barrier and are responsible for various physiological functions. It plays a role in blocking blood clots by producing anti-thrombotic substances such as heparin sulfate, antithrombin and protein, and at the same time, von willebrand factor, fibronectin and thromboplastin ( It also promotes coagulation and thrombosis by releasing substances such as thromboplastin, transports macromolecules to the blood vessel wall, and is also involved in the attachment and recruitment of immune cells. In particular, endothelial cells play a role in maintaining blood flow homeostasis within blood vessels by releasing vascular regulators and regulating blood flow volume and speed, while the main role of red blood cells in the blood is oxygen delivery, which accounts for 30% of red blood cells. Hemoglobin is mainly responsible for red blood cells, which have a diameter of about 8 microns and have a biconcave disc shape, and their shape changes significantly when they pass through capillaries, which is called red blood cell deformability. When oxidation of proteins and fats and changes in cell membrane fluidity occur due to sensitive adult diseases, the deformability of red blood cells is significantly reduced, which can lead to various blood circulation disorders such as reduction in blood flow speed and shear stress. As it continues, it can cause malignant hypertension, heart failure, cerebral hemorrhage, nephrosclerosis, and aortic disease, and the latter is also called a complication of high blood pressure, which is caused by an increase in blood pressure that causes various problems in the blood vessels. Because it can occur multiple times in all blood vessels, it can occur in major organs of the body such as heart, aorta, cerebral veins, kidneys, eyes, etc., resulting in cardiac hypertrophy, heart failure, myocardial infarction, aneurysm, arterial dissociation, and cerebral ischemia. It is a very dangerous cause because it can cause blood clots, cerebral hemorrhage, neuropathy, heart failure, and even blindness, and the incidence rate is actually very high in Korea.

In the end, blood health and normal blood circulation can be seen as the most basic to the health of the human body.

Meanwhile, as we enter modern society, there is a high need for improved blood circulation due to westernized eating habits, lack of exercise, and extended life expectancy. Accordingly, various drugs such as aspirin, nicergoline, and aspirin have been developed to improve blood circulation disorders. However, currently distributed drugs improve blood circulation through a mechanism that thins the blood or enlarges the blood vessels. The effects of these drugs are not only temporary, but also cause fatal side effects such as headaches, gastrointestinal diseases, and bleeding. There is a limitation that occurs frequently.

Accordingly, the development of health functional food materials with excellent blood flow improvement effects and no side effects on the human body is absolutely required in the biotechnology industry.

KR 10-2022-0083404 A

In one aspect, the purpose of the present invention is to provide a health functional food using a ginseng extract containing a high content of ginseng saponins and polysaccharides.

In one aspect, the purpose of the present invention is to provide a health functional food with excellent blood circulation improvement effect and excellent human stability and safety.

In one aspect, the purpose of the present invention is to provide a composition that is excellent in improving vascular health and blood circulation.

In one aspect, the purpose of the present invention is to improve human health by improving vascular health and blood circulation.

In one aspect, the purpose of the present invention is to provide a composition with excellent sexual function improvement effect.

In one aspect, the purpose of the present invention is to provide a composition that is excellent in improving exercise capacity or reducing fatigue.

In one aspect, the purpose of the present invention is to improve sexual function, exercise ability, and fatigue recovery by improving vascular health and blood circulation.

In one aspect, the purpose of the present invention is to provide a health functional food that can be administered not only to humans but also to animals.

In order to achieve the above object, the present invention provides, in one aspect, a composition comprising rhodiola extract, root bark extract, and butterbur extract as active ingredients, and comprising one or more of the following uses:

i) improving vascular health or improving blood circulation, ii) improving sexual function, iii) improving exercise ability and iv) relieving fatigue.

In addition, in order to achieve the above object, the present invention provides a composition further comprising at least one of a ginseng extract and a ginseng extract.

In addition, in order to achieve the above object, the present invention provides a composition wherein the extraction solvent for the extract contained in the composition includes water, an organic solvent, or an aqueous solution thereof.

In addition, in order to achieve the above object, the present invention provides a composition in which one or more of the improvement of sexual function, improvement of exercise ability, and recovery from fatigue is achieved by improving vascular health or improving blood circulation.

In addition, in order to achieve the above object, the present invention provides a composition in which the rhodiola extract, root bark extract, and butterbur extract are included in a weight ratio of 1:0.1 to 1:0.5 to 1.5.

In addition, in order to achieve the above object, the present invention provides a composition in which the ginseng extract and the ginseng extract are each included in an amount of 10 to 100 parts by weight based on 100 parts by weight of the rhodiola extract.

In addition, in order to achieve the above object, the present invention provides a composition, which is a food composition.

The composition, which is one aspect of the present invention, has excellent effects on improving blood vessel health and blood circulation. The composition can be expected to improve sexual function, exercise function, and fatigue recovery by improving vascular health and blood circulation, as well as improving overall physical function. In addition, the composition according to the present invention is harmless to the body and has no side effects, so it can be taken for a long period of time.

Figure 1 is a diagram showing the measurement results of ginsenoside standard substances of ginseng extract according to the present invention.
Figure 2 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 1,726,284.
Figure 3 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 181,813.
Figure 4 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 40,592.
Figure 5 shows the vascular relaxation effect of the composition according to the present invention.
Figure 6 shows the effect of reducing maximum blood pressure (6a) and stabilizing heart rate (6b) of the composition according to the present invention.
Figure 7 shows the malondialdeyde control effect (7a) and LDH control effect (7b) of the composition according to the present invention.
Figure 8 shows the results of the IIEF survey (8a: increase of more than 3 points, increase of more than 15 points).

Hereinafter, the present invention will be described in detail.

The following description is only an example, and the scope of the present invention is not limited by the following.

In addition, the terms or words used in the specification and claims of the present invention are not to be construed as limited to their ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his/her invention in the best way. It must be interpreted with meaning and concept consistent with the technical idea of the present invention based on the principle that it can be done.

Throughout the specification of the present invention, when a part is said to "include" a certain component, this means that it does not exclude other components but may further include other components unless specifically stated to the contrary. .

Korean ginseng (Panax ginseng C.A. Meyer) is a specialty medicinal plant belonging to the Papaceae family and is a perennial herb that grows about 50 to 80 cm in height. Korean ginseng has been widely used as a nutritional tonic for a long time, and with the recent development of analytical science, research on the types and functions of ingredients contained in Korean ginseng is actively underway. Representative physiologically active substances of Korean ginseng include ginsenoside, a ginseng saponin of the triteropenoid family, polyacetylene with anticancer activity, phenolic compounds with antioxidant activity, and radiation protection. It contains ginseng proteins, acidic polysaccharides with immunomodulating properties, and other nutrients such as vitamins, sugars, and minerals, and is known to have excellent rejuvenation, immunity enhancement, nutritional tonic, and anti-cancer effects. .

In the case of ginseng saponins, they are called ‘ginsenosides.’ This is a compound word combining 'ginseng', meaning ginseng, and 'glycoside', meaning ginseng glycoside. Unlike common herbal saponins, ginseng saponins have a dammaran skeleton of triterpenoids, so while saponins derived from other plants are polar and have toxicity such as hemolysis, they are non-toxic and have pharmacological effects because they are neutral glycosides. Unlike the saponins of this common herbal medicine, it has a wide variety of physiological activities. Ginseng saponin components are largely divided into the panaxadiol family, which has a sedative effect, and the panaxatriol family, which has a stimulating effect. Korean ginseng contains more types of saponins than foreign ginsengs such as American ginseng, Panax ginseng, and Jukjeol ginseng, and the panaxadiol family. It is known to have an excellent role in regulating the balance of the body, such as blood pressure and body temperature, due to its balanced ratio of panaxatriol and panaxatriol. Saponin is a component widely found in over 750 species of plants, and is named so because it produces fine bubbles like soap in an aqueous solution. Ginseng saponin is a compound in which sugars such as glucose, xylose, arabinose, and rhamnose are ether bonded to certain regions of the non-saccharide parts PPD and PPT, and its molecular weight is approximately between 800 and 1,200. Ginsenosides are divided into dozens of types depending on the sugar bonding structure. Depending on the difference in migration distance in thin layer chromatography (TLC), ginsenoside Ro, ginsenoside Ra, ginsenoside Rb1, ginsenoside Rb2, ginsenoside They are named ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rg2, ginsenoside Rg3 and ginsenoside Rh1.

Known effects of ginseng saponins include enhancing the anti-decomposition effect of insulin, anti-lipolysis action, and improving symptoms associated with diabetes. Triol-based saponins in ginseng have a somewhat positive effect on the development of type 1 diabetes, and diol-based saponins have an anti-inflammatory effect. It delays the onset of type 1 diabetes and significantly reduces the incidence. It not only increases the insulin content in the pancreas and blood, but also suppresses the infiltration of lymphocytes into the pancreas. Ginseng saponin ginsenoside Rb2 suppresses urinary albumin excretion, suppresses kidney weight and enlargement of glomeruli. Regarding the anti-cancer effect, ginseng saponin increases the body's resistance by increasing the immune function of cancer patients, thus inhibiting the growth of cancer cells and metastasis to other organs, and showing excellent efficacy in preventing recurrence after surgery. It is known, and according to the research results of Murata et al., ginseng concentrate containing a large amount of saponin was administered to cancer patients such as stomach cancer, pancreas cancer, colon cancer, and breast cancer for a long period of 3 to 6 months, and as a result of clinical observation, 69.7% of the patients showed cancer. It was confirmed that the progression and development of was suppressed. In addition, Gusan et al. administered ginseng saponin to various cancer patients and confirmed the effect of improving subjective symptoms, recovering blood, and suppressing cancer progression. According to the results of investigating the relationship between the frequency of taking ginseng and the probability of developing cancer, long-term use of ginseng was found to be effective in suppressing cancer progression. The research results showing that the probability of developing cancer significantly decreases the more you use it, it can be seen that there is a long-term non-specific cancer prevention effect. In addition, ginseng saponin can be said to be a mysterious elixir because it normalizes blood pressure by raising it in case of low blood pressure and lowers blood pressure in high blood pressure, thus maintaining the blood pressure at a normal level. Among ginseng saponins, it is a component that has the effect of raising blood pressure. (Rg group) and components with blood pressure lowering effects (Rb, Rc group) coexist, which acts to normalize blood pressure overall. Taking Korean ginseng in patients with high blood pressure reduces systolic and diastolic blood pressure, lowers low-density cholesterol in the blood in patients with hyperlipidemia, improves lipid metabolism by raising high-density cholesterol, which plays a good role in cholesterol metabolism, and improves endothelial damage caused by high blood pressure. Improves cellular dysfunction. Since the blood pressure lowering effect of ginseng is mainly due to ginseng saponin, the effect is greater when taken in relatively large quantities rather than in small amounts by selecting ginseng concentrate with a relatively high saponin content. Ginseng saponin has the effect of lowering blood pressure, causing parasympathetic It normalizes the balance of the autonomic nervous system by relieving nerve tension as needed, promoting blood flow and smoothing the supply of blood to the brain, making it effective in treating low blood pressure. In addition, ginseng saponins have a vasodilating effect, improving blood circulation, inhibiting the development of arteriosclerosis and protecting against damage to vascular endothelial cells. Ginseng saponins have enzyme activities related to lipid metabolism such as fat and blood cholesterol. It has the effect of promoting and preventing the formation of atheroma caused by hyperlipidemia in the aortic tissue, and has the effect of improving hyperlipidemia, an important risk factor for arteriosclerosis, by promoting cholesterol turnover. Ginseng saponin components (especially Rb2) are effective in reducing blood cholesterol content and promoting excretion, preventing vascular lesions caused by hypercholesterolemia, and improving arteriosclerosis indicators by reducing LDL-cholesterol and increasing HDL-cholesterol. It has been proven.

Meanwhile, ginseng extract contains various sugars. Polysaccharides, which are monosaccharides including glucose and fructose, disaccharides, trisaccharides, tetrasaccharides, pentasaccharides including maltose and saccharides, and many monosaccharides combined with each other, are also found in ginseng (Ovodov et al. 1966). In early research until the 1960s, little chemical research was conducted on ginseng polysaccharides, which are polymeric substances, and little research was conducted on the physiological functions of ginseng saccharides. However, with the recent development of life sciences, carbohydrates have been used as simple biological structural materials and As it becomes known that it plays an important role not only as an energy source but also as a signal transmitter for biological functions, its importance is being re-recognized and research on it is intensifying. In particular, polysaccharides and their derivatives from plants and microorganisms are being developed as immunomodulatory substances, and oligosaccharides are also being actively developed as medical agents such as anti-inflammatory agents and antibacterial agents and functional food materials.

Research on the polysaccharide components of ginseng and their effectiveness was pursued much later than saponin research due to the structural complexity of these components. In 1984, Hikino and Tomoda of Japan were the first to isolate and report the polysaccharide components panaxan A, B, C, D, and E from Korean Korean ginseng (Tomoda et al. 1984, 1985, Konno et al. 1984). Afterwards, as researchers continued to study, a total of 21 species of panaxan, from panaxan F to panaxan U, were isolated (Konno et al. 1985, 1987, Hikino et al. 1985, Oshima et al. 1985). Meanwhile, these polysaccharide components are combined with the same or different monosaccharides, and the main constituent sugars differ depending on the type of polysaccharide, but include glucose, arabinose, galactose, xylose, and rhamnose, etc. Among them, panaxan A is composed of glucose (92.1%) and a small amount of peptide (1.7%) and has a molecular weight of approximately 14,000, while panaxan B contains 95.9% glucose and 0.7% peptide and is a peptidoglycan with a molecular weight of approximately 1,800,000. The structure is being revealed (Tomoda et al. 1984, 1985). It has been revealed through animal experiments that the physiological activity of these polysaccharide components has a hypoglycemic effect that significantly suppresses hyperglycemia induced by treatment with alloxan, an experimental diabetes-inducing substance (Konno et al. 1984). , Ng et al. 1985). Yang et al. also reported that when polysaccharide components isolated from ginseng were administered to experimental animals (rats) (50-200 mg/kg, intraperitoneal, subcutaneous injection), they lowered blood sugar levels, reduced liver glycogen content, and promoted insulin secretion ( Yang et al. 1990). Additionally, there are reports that polysaccharide components isolated from ginseng improve immune function. It is known that the anti-tumor activity of most polysaccharide components is not directly toxic to cancer cells, but is due to an immune-modulating effect. Currently, in China, considering that the polysaccharide fraction of ginseng is used clinically as an anticancer agent, attempts are being made to isolate polysaccharide components from ginseng roots and leaves and study their pharmacological activity. Wang et al. administered ginseng polysaccharide components orally or intraperitoneally to normal and cancer-causing animals (mice) and found that it had the effect of enhancing the foreign substance phagocytosis ability of the reticuloendothelial system, which is responsible for immune function, and promoting antibody production. It was also reported that these ingredients had an improvement effect in clinical application to cancer patients (Wang et al. 1985). The water-soluble and alkaline (0.5 M NaOH)-soluble polysaccharide fractions isolated from the roots and leaves of ginseng have anti-complementary activity, and among these fractions, the acidic polysaccharide fraction showed the strongest activity ( Gao et al. 1989). The complement system plays an important role in immunity, inflammation, and allergic reactions, and polysaccharides with anti-tumor effects are reported to have anti-complement activity (Okuda et al. 1972). The content of crude acidic polysaccharides is higher in roots than in leaves. Their chemical properties are different; the roots mainly contain pectin and glucan, while the leaves contain pectin and heteroglycan (Gao et al. 1989). However, the chemical structure of these acidic polysaccharides was not revealed, and through further advanced research, two new acidic polysaccharide components, namely ginsenan PA and ginsenan PB, were recently isolated from water-soluble extracts of Korean ginseng, which have remarkable anticomplement activity. It has the effect of enhancing the activity of the intrareticulum system, which is responsible for immune function, by phagocytosing foreign substances coming from the outside (Tomoda et al. 1993). The molecular weights of these ingredients are approximately 16,000 and 5,500, respectively, and their chemical structural forms have structural units of the α-arabino-*?*-3,6-galactan type and rhamno galacturonan type. Ginsenan PA is composed of L-arabinose, D-galactose, L-rhamnose, D-galacturonic acid (11:22:1:6:1, molar ratio) and 1.3% O-acetyl group, which are decomposition products of ginsenan PA. As a result of examining the phagocytic activity and anti-complement activity of In Korea, research on the efficacy of ginseng polysaccharide components in relation to cancer immunity is being pursued with interest. Regarding immunotoxicity of cyclosphamide (CY), an anticancer drug, ginseng polysaccharide fraction administered to mice was effective in suppressing immunotoxicity (reduction in number of hemolytic plaque forming cells, number of white blood cells, spleen weight, etc.) caused by CY treatment (Kim et al. al, 1990). In addition, administration of ginseng polysaccharide components suppressed the development of cancer in mice transplanted with cancer cells (Sarcom-180) and showed an increase in hemolytic plaque forming cells (PFC) related to antibody production and an enhancement of phagocytic activity in the reticular system. Strong activity was observed in the acidic polysaccharide fraction (Kim et al. 1990, 1991). Meanwhile, Okuda et al. in Japan isolated acidic polysaccharides from water extracts of Korean red ginseng through research on physiologically active substances other than saponin contained in Korean red ginseng over the past 10 years. The chemical structure of this ingredient was found to have an α-1,4-polygalturonan skeleton similar to pectin with several acetoxy groups (Lee et al. 1990, Okuda 1990). In general, cancer causes weight loss, and it is known that the weight loss of cancer patients is due to the decomposition of body fat and the effect of causing loss of appetite by toxohormone-L secreted by cancer cells. The acidic polysaccharide component of ginseng causes this toxohormone. (Okuda et al. 1984, Okuda 1989). In addition, these acidic polysaccharide components inhibit pancreatic lipase (lipolytic enzyme) activity and cholesterol esterase activity and reduce serum neutral lipids. It has been shown to be active. Since this action can inhibit or delay the absorption of sugars or fats from the intestines, it is expected to be effective in preventing so-called obesity or hyperlipidemia (Okuda et al. 1993). Briefly describing the results of research on ginseng polysaccharide at the Korea Atomic Energy Research Institute, ginseng polysaccharide increases the ability of macrophages to produce IL-1 and IL-12 in the biological immune mechanism, and the action of this factor increases IL-2 production in Th cells. As the ability to produce cytokines such as and IFN-γ increases, the responsiveness of various cells to these cytokines increases, and the activity of cytotoxic T cells, LAK cells, NK cells, macrophages, etc. increases. Additionally, by proliferating bone marrow cells, it reduces side effects caused by various anticancer drugs or radiation that cause bone marrow failure. Looking at the anti-cancer immune enhancement effect more specifically, the production of nitric oxide (NO) in the culture medium was increased when mouse intraperitoneal macrophages were cultured in dianic acid-added FCS-RPMI1640 medium, and mouse intraperitoneal macrophages were cultured in dianic acid-added FCS-RPMI1640 medium. When cultured with tumor cells Yac-1 for 48 hours, the growth of Yac-1 cells was inhibited. When 100 mg/kg of ginseng polysaccharide was intravenously administered to Balb/c mice, NK activity increased 2.4 times compared to the non-administered group, and the proliferation ability of T lymphocytes increased when 100 mg/kg of ginseng polysaccharide was intravenously administered to Balb/c mice. Responsiveness of cells to mitogen (Concanavalin A 2.5㎍/ml) increased 2.1-fold. In the case of LAK cell production ability, mouse spleen cells were cultured in vitro in FCS-RPMI1640 medium supplemented with 0.5㎍/㎖ of ginseng polysaccharide. The killing activity against tumor cell YAC-1 was measured and was 10 times stronger than that of the control group. Ginseng polysaccharide not only exhibits anti-cancer immune activity by promoting the secretion of various types of cytokines, but also functions to maintain the normal state when the autonomous balance of various cytokines is abnormal, and Th1 type cytokines (IL-2, (IFNγ) and Th2 type (IL-4, IL-5, IL-10) cytokines are maintained in balance. In diseases such as asthma and allergies, Th2 secretion increases, and in rheumatoid arthritis, Th1 secretion increases. It is caused by the assembly and reaction of cells, and the regulation of these cytokines can be said to be a very important factor. When mouse spleen cells were cultured in FCS-RPMI1640 medium supplemented with 50㎍/㎖ of ginseng polysaccharide, IL-1, IL-12, The mRNA expression of IL-2, IFN, CSF, and TNFα increased, and when 100 mg/kg of ginseng polysaccharide was administered intravenously to Balb/c mice, the production capacity of cytokines such as GM-CSF, IL-12, IFNγ, and TNFα in the serum was increased. This has increased significantly. When B16-F10 melanoma, a type of lung cancer cell, was injected intraperitoneally 3 days later, 10 mg/kg of ginseng polysaccharide was injected intraperitoneally eight times every other day, metastasis of cancer cells to the lung was inhibited by 44%. When the LLC lung cancer cell line was subcutaneously injected into mice and the size of 1cm3 was reached, 100 mg/kg of ginseng polysaccharide was administered intravenously to inhibit the growth of 59.3% of cancer cells. The 30-day survival rate of mice transplanted with the LLC lung cancer cell line was 12.5% in the control group. It increased to 80% in the ginseng polysaccharide administered group. In addition, looking at the number of bone marrow cells on the 5th day of mice injected intravenously with 100 mg/kg of ginseng polysaccharide and then irradiated with a sublethal dose, there was significant recovery of myeloblasts and recovery of differentiated granular-mononuclear cells. Confirmed. Hematopoietic progenitor cells undergo a process of maturation and differentiation into granulocytes, lymphocytes, erythrocytes, platelets, etc. under the influence of various hematopoietic factors and the bone marrow microenvironment. In general, inflammation and infection due to bone marrow failure occur during radiation treatment in cancer patients. This is a common cause of side effects, but when 100 mg/kg of ginseng polysaccharide was injected intravenously, the production capacity of white blood cells, neutrophils, lymphocytes, and platelets in the peripheral blood was increased, and the radiation exposure of mice administered 100 mg/kg of ginseng polysaccharide 24 hours before radiation exposure was increased. The lethal dose (LD50/30) was 7.46 Gy in the control group to 10.76 Gy in the ginseng polysaccharide administered group, increasing the survival rate of bone marrow cells by 1.44 times. Therefore, ginseng polysaccharide can increase the effectiveness of ginseng when used in combination with radiation, both in terms of increasing the radiation therapy dose for cancer treatment and reducing side effects. In addition, the 20-day survival rate of mice administered 100 mg/kg of ginseng polysaccharide intravenously 48 hours before cyclophosphamide (intraperitoneal injection) increased from 20% in the control group to 60% in the ginseng polysaccharide-administered group, indicating the significance of granulocytes in peripheral blood due to ginseng polysaccharide administration. It is thought that this increase improves the side effects of anticancer drugs such as cyclophosphamide.

The present invention relates to ginseng saponin ingredients (ginsenosides), which are notified health functional ingredients recognized as being effective in improving immunity, relieving fatigue, and improving blood circulation among the ingredients contained in Korean ginseng, and non-ginseng saponin-based substances, Ginseng polysaccharide component, which has recently been recognized as an important substance in the field of alternative medicine and has been proven to have excellent efficacy in the field of biological immune response modulators, which is rapidly growing globally, and Korean ginseng extract that controls both components simultaneously as an indicator component. It is named Panaxana Ginsan.

"Rhodiola Linne" is a perennial plant belonging to the Rhodiola Linne genus of the Crassulaceae family. The main active ingredients contained in Rhodiola rosea are known to include ketones, agricone, and tyrosol, which have excellent nourishing and tonic effects. In Japan, basic research at Kyoto University of Pharmacy has revealed that the glycoside glycosides contained in Rhodiola rosea are effective against atopy and inhibit the production of melanin, which causes spots and freckles.

Approximately 40 types of active ingredients contained in Rhodiola rosea have been identified in research so far. Research has shown that basic ingredients such as lipids, proteins, essential amino acids, various vitamins, organic acids, and minerals, as well as 'Rhodiola persimmon' discovered by Russia's Chausk Medical University, have the effect of normalizing muscles and brain under long-term tension and enhancing immune function. The results are out. In this way, Rhodiola rosea has the effect of regulating the central nervous system and endocrine system and increasing immunity, and its known effects so far are anti-aging, recovery from fatigue, high blood pressure, cancer, viral hepatitis, liver disease, leukemia, and recovery of exercise ability in a low-oxygen environment. (recovery from fatigue), etc. Additionally, rhodiola is rich in zinc. Zinc is known to promote the production, development, and motility of sperm and eggs. In particular, arginine (which increases the number of sperm) and lysine (which increases the number of sperm), which are essential amino acids of Rhodiola rosea (an amino acid that is not produced in the body and must be supplied from outside) The abundance of (increasing the revision rate) is also an additional factor. Therefore, when we take Rhodiola rosea, it normalizes our body's endocrine (hormonal) activities, helps us find stability in mind and body through its strong anti-stress effect, and improves sexual function through a nutritious and tonic effect that is not difficult at all, contributing sufficiently to overcoming infertility. You can.

“Ulmus davidiana var. japonica” is the root bark of the elm tree ( Ulmus davidiana var. japonica ). Elm tree is a deciduous broad-leaved tree of the elm family of the dicotyledonous plant Nettle family. It is also called Chunyu (春楡) or Gayu (家楡). The height of the elm tree is 20 m, the diameter is 60 cm, the bark is gray-brown, and the small branches have short red-brown hairs. Young leaves are used as food in spring, and the bark is used as a medicine called dermoid, and is known to be used as a toothache, diuretic, and venom poison.

In Donguibogam, Yugeunpi is mentioned to help with diuresis and reduce swelling. In addition, it has excellent anti-inflammatory and antibacterial effects, and is known to be especially effective in treating nose-related diseases such as rhinitis and sinusitis.

" Saururus chinensis (Lour.) Baill.)" is a perennial herb that grows in parts of Jeju Island and Jiri Mountain. The growing environment is that it grows in a humid valley with good ventilation, high air humidity, and partial shade. The height is 50 to 100 cm, and the leaves are 5 to 15 cm long and 0.3 to 0.8 cm wide, have a long oval shape and grow alternately. The surface of the leaf is light green and the back is light white. By the time the flower blooms, 2-3 upper leaves turn white, have 5-7 veins, have a sharp tip, and have a plain edge. The roots are white and burrow into the soil and grow sideways. The flowers are white, drooping down, and the tip goes up, facing the leaves, and are 10 to 15 cm long and have curly hairs. Fruits hang in a circle from September to October, one at a time, on each flower bud. It is used for ornamental purposes, and the leaves, stems, and roots, including the flowers, are used as medicine.

By facilitating the excretion of sodium from the body, ginseng prevents vascular diseases such as arteriosclerosis and high blood pressure. It purifies the blood by expelling toxins accumulated in the body, improving the constitution of skin problems such as acne and atopy, and reducing inflammation in the bronchial tubes. Helps relieve phlegm and cough by removing it.

In addition, ginseng helps in the synthesis of collagen, suppresses aging of blood vessels and strengthens them, and contains a lot of potassium, which has the effect of smoothly discharging urine. In addition, trichomes are effective in suppressing inflammation by treating inflammation or wounds of the stomach lining weakened by a body exhausted from stress and bad eating habits, and are also excellent for improving immunity and detoxification. In oriental medicine, it is used for vascular diseases, body swelling, and difficulty urinating, and is also prescribed for beriberi, jaundice, and hepatitis.

"Butterbur" is a perennial herb plant belonging to the dicotyledonous plant Campanula Asteraceae. Its leaves, stems, and roots are all rich in vitamins and minerals that are beneficial to the human body. Butterbur contains an ingredient called choline, which has the effect of detoxifying toxins. Therefore, eating it with fish that has a high risk of food poisoning can prevent food poisoning and help relieve intestinal toxins. Additionally, drinking water steeped in butterbur helps improve and prevent bronchial diseases such as tonsillitis, asthma, and cough. Butterbur has the effect of discharging bad cholesterol in the blood vessels, which helps prevent and improve vascular diseases such as hyperlipidemia and arteriosclerosis, and because it is very rich in calcium, it has the effect of strengthening bones, making it prone to osteoporosis. It is good for menopausal women and the elderly and helps build strong bones in growing children. In addition, according to research results published in Switzerland, butterbur is known to be effective in relieving pain in cancer patients and treating inflammation, and is rich in ingredients that are helpful in anti-cancer, such as polyphenols. In addition, butterbur is known to be excellent for neutralizing acid constitution, relieving headaches, and improving diuresis in addition to the above effects.

In one aspect, the present invention is a composition comprising rhodiola extract, root bark extract, and butterbur complex extract as active ingredients.

In one aspect, the composition of the present invention may include one or more of the following uses: i) improving vascular health or improving blood circulation, ii) improving sexual function, iii) improving exercise capacity, and iv) relieving fatigue.

In this specification, “blood vessel” is a passage through which blood circulates throughout the body, and is used in a broad sense to include arteries, veins, and capillaries.

In this specification, “extract” includes all substances obtained by extracting components of natural products, regardless of the extraction method, extraction solvent, extracted component, or form of the extract. In addition, it is a broad concept that includes all substances that can be obtained by extracting the components of natural products and processing or treating them by other methods. Specifically, the processing or treatment refers to additional fermentation or enzyme treatment of the extract. You can. Therefore, in this specification, extract is a broad concept including fermented product, concentrate, and dried product.

In one aspect, the improvement of sexual function may include improvement of female or male sexual function, but in particular, it may include improvement of male sexual function, such as improving erectile dysfunction.

In one aspect, the rhodiola extract, root bark extract, and butterbur extract may be included in a weight ratio of 1: 0.1 to 1: 0.5 to 1.5.

Specifically, the rhodiola extract, root bark extract, and butterbur extract are 1:0.1~0.8:0.6~1.4, or 1:0.1~0.7:0.6~1.3, or 1:0.1~0.5:0.7~1.3, or 1:0.1. It may be included in a weight ratio of ~0.4:0.8~1.3, or 1:0.1~0.3:0.8~1.2, or 1:0.1~0.3:0.9~1.2, or 1:0.1~0.3:0.9~1.1.

In one aspect, the radicle bark extract is 10 to 100 parts by weight, or 10 to 90 parts by weight, or 20 to 90 parts by weight, or 30 to 90 parts by weight, or 30 to 80 parts by weight, based on 100 parts by weight of the rhodiola extract. Alternatively, it may be included in 30 to 70 parts by weight, or 40 to 60 parts by weight.

In addition, the butterbur extract is 10 to 100 parts by weight, or 10 to 90 parts by weight, or 10 to 80 parts by weight, or 10 to 70 parts by weight, or 10 to 60 parts by weight, or 10 to 100 parts by weight, based on 100 parts by weight of the rhodiola extract. It may be included in ~50 parts by weight, or 10-40 parts by weight, or 10-30 parts by weight.

In one aspect, the composition may further include one or more of a ginseng extract and a ginseng extract.

In one aspect, the ginseng extract included in the present invention contains 65% by weight or more of polysaccharides, based on the total weight of the extract; and 10% by weight or more of saponin, and the polysaccharide of the ginseng extract is a ginseng extract containing polysaccharides with a molecular weight of 3,500 to 2,000,000 in an amount of 55% or more based on the total weight of the polysaccharide.

In one aspect, it is generally known that about 95% of the polysaccharide contained in ginseng is composed of glucose.

In the above aspect, the content of glucose contained in the ginseng extract of the present invention may be 60% or more, based on the total weight of the extract. In one embodiment, the total content of glucose contained in the ginseng extract of the present invention may be 69.3%.

In addition, in one aspect, the content of polysaccharides contained in the ginseng extract of the present invention may be 65 to 75% by weight, or 67 to 75% by weight, or 67 to 73% by weight, or 67 to 71% by weight, or 68% by weight. It may be ~70% by weight.

In addition, in one aspect, the content of saponin contained in the ginseng extract of the present invention is 10 to 20% by weight, or 13 to 20% by weight, or 13 to 18% by weight, or 15 to 18% by weight, based on the total weight of the extract. It may be %.

In addition, in one aspect, the polysaccharide having a molecular weight of 3,500 to 2,000,000 among the polysaccharides included in the ginseng extract of the present invention is 55 to 75% by weight, or 55 to 65% by weight, or 57 to 65% by weight, based on the total weight of the polysaccharide. %, or 59 to 65% by weight, or 59 to 63% by weight, but is not limited thereto.

Additionally, in one aspect, the ginseng extract may include polysaccharides with a molecular weight of 35,000 to 45,000 in an amount of 15 to 20% by weight based on the total weight of the polysaccharides. Specifically, polysaccharides having a molecular weight of 35,000 to 45,000 may be included at 16 to 19% by weight, or 16 to 18% by weight, based on the total weight of the polysaccharide.

In addition, in one aspect, the ginseng extract may contain polysaccharides with a molecular weight of 160,000 to 200,000 in an amount of 17 to 23% by weight relative to the total weight of the polysaccharides. Specifically, polysaccharides with a molecular weight of 160,000 to 200,000 may include 17 to 23% by weight relative to the total weight of the polysaccharides. It may be included in weight%, or 18 to 20 weight%.

In addition, in one aspect, the ginseng extract may contain polysaccharides with a molecular weight of 1,500,000 to 2,000,000 in an amount of 0.7 to 1.2% by weight relative to the total weight of the polysaccharides, specifically, 0.7 to 1.1% by weight, or 0.7 to 1.0% by weight, or It can be included at 0.8 to 1.0% by weight.

In addition, in one aspect, the ginseng extract may include one or more of the following characteristics: i) the polysaccharide having a molecular weight of 35,000 to 45,000 contains 70% by weight or more of glucose or 3% by weight or less of glucose relative to the molecular weight of the polysaccharide. Contains protein, ii) Polysaccharides with a molecular weight of 160,000 to 200,000 contain more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide, iii) Polysaccharides with a molecular weight of 1,500,000 to 2,000,000 contain the corresponding Contains more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide.

Specifically, the polysaccharide having a molecular weight of 35,000 to 45,000 may contain more than 70% by weight of glucose based on the molecular weight of the polysaccharide. Specifically, the polysaccharide having a molecular weight of 35,000 to 45,000 may contain more than 73% by weight of glucose, or more than 75% by weight of glucose based on the molecular weight of the polysaccharide. For example, the content of glucose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 75 to 83% by weight, or 77 to 81% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 35,000 to 45,000 contained in the ginseng extract may further include galactose, araminose, and rhamnose. The content of galactose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 2 to 8% by weight, or 2 to 6% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 5 to 15% by weight, or 7 to 15% by weight, or 9 to 13% by weight, based on the total weight of the polysaccharide. Or it may be 10 to 12% by weight. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 1 to 6% by weight, or 2 to 6% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 160,000 to 200,000 contained in the ginseng extract may contain 80% by weight or more of glucose relative to the molecular weight of the polysaccharide. Specifically, the content of glucose contained is, It may be 85 to 95% by weight, or 87 to 95% by weight, or 87 to 93% by weight, or 89 to 93% by weight, relative to the molecular weight. In addition, the polysaccharide with a molecular weight of 160,000 to 200,000 contained in the ginseng extract may further include galactose, araminose, and rhamnose. The content of galactose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 0.5 to 5% by weight, or 0.5 to 4% by weight, or 1 to 3% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 0.1 to 2% by weight, or 0.1 to 1.5% by weight, or 0.1 to 1% by weight, based on the total weight of the polysaccharide. Or it may be 0.3 to 0.7 weight%. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 1 to 10% by weight, or 3 to 9% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract may contain more than 80% by weight of glucose relative to the molecular weight of the polysaccharide. Specifically, the content of glucose contained is, It may be 85 to 95% by weight, or 87 to 95% by weight, or 87 to 93% by weight, or 88 to 92% by weight, relative to the molecular weight. In addition, the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract may further include galactose, araminose, rhamnose, and xylose. The content of galactose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 5 to 15% by weight, or 7 to 13% by weight, or 7 to 11% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.01 to 0.1% by weight, or 0.01 to 0.1% by weight, or 0.01 to 0.07% by weight, based on the total weight of the polysaccharide. Or it may be 0.01 to 0.05% by weight. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.001 to 0.1% by weight, or 0.005 to 0.015% by weight, or 0.007 to 0.013% by weight, based on the total weight of the polysaccharide. In addition, the content of xylose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.1 to 0.5% by weight, or 0.1 to 0.3% by weight, or 0.1 to 0.2% by weight, based on the total weight of the polysaccharide.

In addition, each of the polysaccharides with a molecular weight of 35,000 to 45,000, the polysaccharide with a molecular weight of 160,000 to 200,000, and the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract of the present invention may contain proteins. The protein content contained in each of these may be 3% by weight or less based on the total weight of the polysaccharide, and in particular, the protein content contained in the polysaccharide with a molecular weight of 35,000 to 45,000 is 2% by weight based on the total weight of the polysaccharide. It may be below.

Additionally, in one aspect, the ginseng extract of the present invention may contain Rg 1 and Gb 1 in an amount of 2.5% by weight or more based on the total weight of the extract. Specifically, the total amount of Rg 1 and Gb 1 contained in the ginseng extract may be 2.5 to 3% by weight, or 2.5 to 2.8% by weight, or 2.5 to 2.7% by weight, based on the total weight of the extract.

In one embodiment, the polysaccharide having a molecular weight of 1,726 or 284 may be included in an amount of 0.5 to 1% by weight, or 0.7 to 1% by weight, or 0.85 to 0.95% by weight, based on the total weight of the polysaccharide.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 562,356 may be included in an amount of 3.5 to 5% by weight, or 3.5 to 4.7% by weight, or 4 to 4.7% by weight, or 4 to 4.5% by weight, based on the total weight of the polysaccharide. there is.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 181,813 may be included in an amount of 17 to 23 wt%, or 18 to 22 wt%, or 19 to 20 wt%, based on the total weight of the polysaccharide.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 40,592 may be included in an amount of 13 to 21% by weight, or 14 to 20% by weight, or 15 to 19% by weight, or 16 to 18% by weight, based on the total weight of the polysaccharide. there is.

In addition, in one embodiment, the polysaccharide having a molecular weight of 6,038 may be included in an amount of 5 to 11% by weight, or 6 to 11% by weight, or 7 to 10% by weight, or 7 to 9% by weight, based on the total weight of the polysaccharide. there is.

In addition, in one embodiment, the polysaccharide having a molecular weight of 3,555 is 8 to 15% by weight, 9 to 15% by weight, 9 to 13% by weight, or 10 to 13% by weight, or 11 to 13% by weight, based on the total weight of the polysaccharide. It may be included in weight percent.

In one aspect, the ginseng extract of the present invention can exhibit excellent efficacy by containing the above substances as described above.

In one aspect, the method for producing a ginseng extract included in the present invention includes the step of extracting ginseng; And it may include a ginseng foil extraction step.

As used herein, “ginseng powder” may refer to the residue remaining after first extracting the active ingredient from ginseng whole plant.

In other words, the ginseng foil extraction step may refer to the step of extracting the remaining ginseng residue after performing the ginseng outgrowth extraction step.

In one aspect, the ginseng whole plant extraction step and the ginseng foil extraction step may include being performed by solvent extraction.

The solvent used in the ginseng whole plant extraction step may include water, and the solvent used in the ginseng foil extraction step may include one or more of an organic solvent and an aqueous solution thereof.

In one aspect, the ginseng whole plant extraction step may be performed including one or more of the following conditions: i) extraction temperature: 80 to 95°C, ii) extraction time: 6 to 12 hours, iii) amount of extraction solvent ( volume): 3 to 8 times the weight of ginseng whole plant.

For example, the ginseng whole plant extraction step can be performed for 6 to 12 hours using water at a temperature of about 85 to 95 ° C as a solvent, and at this time, the extraction solvent is added about 3 to 8 times based on the weight of the ginseng whole plant. It can be done.

In one aspect, the ginseng whole plant extraction step may further include washing the ginseng, and crushing the ginseng. The crushing step may include being performed so that the particle size of the ginseng shredded material is about 2 to 5 mm.

Additionally, in one aspect, after the ginseng whole plant extract step is performed, the step of separating the ginseng whole plant extract and the ginseng powder may be further included. At this time, the step of separating the ginseng foil may include cooling the temperature of the ginseng extract to about 50° C. or lower and then separating the ginseng foil and the ginseng extract using centrifugal dehydration or a filter press.

In addition, the ginseng foil extraction step may be performed under one or more of the following conditions: i) extraction temperature: 50°C or less, ii) concentration of extraction solvent: 60-90% (v/v) aqueous alcohol solution and iii) Extraction time: 3 to 6 hours.

In one aspect, the organic solvent used in the ginseng peel extraction step is not particularly limited, and is a low C ₁ to C ₅ solvent such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol, and isobutanol. Alcohol, polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol, hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, and chloroform, and petroleum It may be a non-polar organic solvent such as ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether, and ethyl acetate.

In one embodiment, the solvent used in the ginseng peel extraction step of the present invention may be an aqueous ethanol solution.

The concentration of the ethanol aqueous solution may be 50 to 90% (v/v), 60 to 90% (v/v), or 70 to 80% (v/v).

Additionally, in one aspect, the ginseng foil extraction step of the present invention may be performed using low-temperature extraction, for example, using a solvent below about 50°C, and may be performed for about 3 to 6 hours.

In one aspect, the method may further include removing the extraction solvent contained in the extract obtained in the ginseng foil extraction step. For example, the solvent (ethanol) can be removed by distillation at 50-70°C in a reduced pressure environment.

Additionally, in one aspect, the method for producing the ginseng extract may further include mixing the extract obtained from the ginseng whole plant extraction step and the extract obtained from the ginseng foil extraction step.

Additionally, in one aspect, the method for producing a ginseng extract of the present invention may further include the step of removing substances other than the target substance. Specifically, heavy metal substances that may be present in the extract can be removed by passing the extract through a filtration device (e.g., activated carbon filtration device), and the temperature of the extract can be adjusted to remove proteins, carbohydrates, and fibers floating in the extract. After cooling to 5°C or lower, centrifugation can be performed at a speed of 15,000rpm or higher.

In addition, a filtration process can be further performed to remove microorganisms (bacteria, etc.) and inactive polysaccharides with a molecular weight of 2,000,000 or more in the ginseng extract. The filtration process can be repeated several times by passing through a system equipped with a TFF (tangential flow filtration) membrane with a size of 0.2 micrometers.

Additionally, in one aspect, the method may include concentrating the extract. The concentration step can be performed in a reduced pressure concentration device, and the concentration is performed at a temperature of about 75 to 85 ° C until the sugar content reaches about 8 to 10 Brix.

Meanwhile, during the concentration process, proteins dissolved in the extract may be leached, which can be removed through centrifugation, etc. as described above.

In one aspect, the ginseng extract prepared by the above method may be formulated in powder form or liquid form. For example, the ginseng extract can be frozen at a temperature of about -60°C, then heated and maintained several times in a vacuum for 48 hours, and then powdered while raising the temperature to 40°C. In the case of liquid, it can be formulated as a concentrate to have a sugar content of 10 to 65 Brix using a reduced pressure concentration device at a concentration temperature that does not exceed 95°C.

In one aspect, when the manufacturing method of the present invention is followed as described above, a large amount of active ingredients can be extracted from ginseng in a non-difficult manner, making it economical.

The ginseng extract and the ginseng extract may each be included in an amount of 10 to 100 parts by weight, based on 100 parts by weight of the rhodiola extract.

Specifically, the ginseng extract is 50 to 150 parts by weight, or 60 to 140 parts by weight, or 70 to 140 parts by weight, or 70 to 130 parts by weight, or 70 to 120 parts by weight, when the rhodiola extract is 100 parts by weight. parts, or 80 to 120 parts by weight, or 90 to 120 parts by weight, or 90 to 110 parts by weight.

In addition, the above-mentioned Chrysanthemum sinensis extract is 10 to 100 parts by weight, or 10 to 80 parts by weight, or 10 to 70 parts by weight, or 10 to 50 parts by weight, or 10 to 40 parts by weight, based on 100 parts by weight of the Chonggyeongcheon extract. , or it may be included in 10 to 30 parts by weight.

In one aspect, the extraction solvent for the extract may include water, an organic solvent, or a mixture thereof.

For example, the organic solvent may be hexane, methylene chloride, alcohol, etc., but is not limited thereto. In the above, water includes distilled water or purified water, and the organic solvent is a group consisting of alcohol including lower alcohols of C ₁ to C ₅ , acetone, ether, ethyl acetate, diethyl ether, methanol, ethyl methyl ketone, and chloroform. Includes, but is not limited to, one or more selected from.

In one aspect, the extraction solvent of the extract may include alcohol, and in this case, the alcohol may include ethanol.

The concentration of the alcohol may be 30 to 99% (w/w), or 30 to 80% (w/w), or 40 to 80% (w/w), or 40 to 70% (w/w), , preferably 40 to 60% (w/w).

In one aspect, the extract is refluxed by soaking in an aqueous solution of about 30 to 80% ethanol, and then distilled in a reduced pressure distillation device under conditions of about 30 to 90 degrees Celsius or 50 to 70 degrees Celsius to obtain an organic extract. can do.

In one aspect, the extract can be obtained by performing both organic solvent aqueous solution extraction and water extraction.

For example, after primary extraction using an organic solvent or aqueous organic solvent solution, secondary extraction using hot or cold water extraction can be performed using water, and after primary extraction with water, extraction with an organic solvent or aqueous organic solvent solution can be performed. You can perform secondary extraction using .

In one aspect, one or more of the improvement in sexual function, improvement in exercise ability, and recovery from fatigue may be due to improvement in vascular health or improvement in blood circulation.

Additionally, in one aspect, the composition may include a food composition, and the food composition may include a health functional food.

Health functional foods are manufactured using nutrients that are easily deficient in daily diet or raw materials or ingredients (functional ingredients) with useful functions for the human body. They maintain and improve health by maintaining the normal function of the human body or activating physiological functions. It may mean food that does, but is not limited to this.

The food composition may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc., but is not limited thereto and may be manufactured and processed in any form in accordance with the law.

The mixing amount of the active ingredient can be appropriately determined depending on the purpose of use (prevention, health, or therapeutic treatment). Generally, when manufacturing food or beverages, each of the extracts of the present invention is added in an amount of 15% by weight or less, preferably 10% by weight or less, based on the raw materials. However, in the case of long-term intake for the purpose of health and hygiene or health control, the amount may be below the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There are no special restrictions on the types of foods above. Examples of foods to which the extract of the present invention can be added include meat, sausages, bread, chocolate, candies, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, There are drinks, alcoholic beverages and vitamin complexes, and it includes all health foods in the conventional sense.

In addition to the above, the extract of the present invention contains various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid. It may contain carbonating agents used in beverages.

The food composition may be administered orally, and for humans, a typical daily dosage of the composition may range from 1 to 100 mg/kg body weight, preferably 5 to 70 mg/kg body weight, once Alternatively, it can be administered in several divided doses. However, it should be understood that the actual dosage of the composition should be determined in light of several relevant factors such as the disease to be treated, the route of administration, the patient's age, gender and weight, and the severity of the disease, and therefore, the dosage is determined by any method. It does not limit the scope of the present invention.

Additionally, in one aspect, the food composition may be for humans, or may be for animals, specifically for livestock, or for pets.

Hereinafter, the present invention will be described in detail through preparation examples and examples. These examples are merely for illustrating the present invention, and the scope of the present invention is not limited by these examples.

[Manufacturing example]

[Preparation Example 1] Preparation of ginseng extract

① Wash the phosphorus ginseng in water to remove any dirt or foreign substances, put it in a crusher, crush it into coarse particles with a particle size of about 2 to 5 mm, place it in an extraction bag, and compare it to the phosphorus ginseng weight. Hot water extraction was performed by adding 3 to 8 times the weight of water, setting the extraction temperature to 85 to 95°C, and setting the extraction time to 6 to 12 hours from the time the extraction temperature was reached.

② After extraction was completed, the temperature of the ginseng hot water extract was cooled to 50°C or lower, and then the extraction bag containing the ginseng foil was pressed to separate the ginseng hot water extract and the ginseng foil.

③ In order to extract as much of the ginseng saponin remaining in the ginseng cucumber as possible, the ginseng cucumber separated in step ② was placed back into the extraction bag, then placed in a low-temperature reflux extraction device, and the solvent was mixed at a volume ratio of ethanol: water of 3:1. After that, the extraction bag containing the ginseng gourd was placed in a low-temperature reflux extraction device to the extent that it was submerged. Low-temperature reflux extraction was performed for about 3 to 6 hours by controlling the extraction temperature of the solvent below 50°C at room temperature and continuously circulating the solvent in a shower-type spray form within the low-temperature reflux extraction device to maximize extraction efficiency.

④ After step ③ is completed, the extraction bag containing the ginseng gourd is removed, the ginseng reflux extract is put into a vacuum distiller, and the ethanol is distilled at a distillation temperature of 50-70°C under reduced pressure, cooled, and recovered to produce the ginseng reflux extract. The alcohol ethanol used as a solvent was removed.

⑤ The refluxed ginseng extract from which the ethanol was removed was mixed with the ginseng hot water extract from step ① to complete the extraction of the ginseng extract desired in the present invention.

⑥ In order to remove heavy metal substances present in the ginseng extract, the heavy metal substances were adsorbed and removed through an activated carbon filtration device. In addition, in order to remove particulates such as proteins, carbohydrates, and fiber floating in the ginseng extract, the ginseng extract is cooled to below 5℃, and when the rotation speed of the ultra-high-speed centrifuge reaches over 15,000 rpm, the cooled ginseng extract is ultra-high-speed centrifuged. The particulates to be removed by putting them into the separator were accumulated in the rotating body, and the ginseng extract was separated and purified while being discharged through the discharge port at the top of the rotating body.

⑦ In order to remove bacterial microorganisms and inactive polysaccharide substances with a molecular weight of 2,000,000 or more present in the ginseng extract, it is permeated after passing through an Ultra-filtering system equipped with a TFF (Tangential Flow Filtration) 0.2㎛ membrane (Millipore). The ginseng extract is collected as the target ginseng extract and passed through the ultra-filtering system. The ginseng extract that is not filtered and retentated is diluted with water several times and filtered through the ultra-filtering system repeatedly several times to recover as much of the target ginseng extract as possible and then discarded. did.

[Preparation Example 2] Preparation of Rhodiola extract

① Cut the dried rhodiola root into powder or pieces of about 2 to 5 mm, put the rhodiola root (piece) into a non-woven bag, and then put it into a circulation-type reflux extraction tank.

② Add 30 to 70% alcohol as an extraction solvent to the circulatory reflux extraction tank to submerge the non-woven bag containing rhodiola, and extract for 12 hours while refluxing the solvent at an extraction temperature not exceeding 40°C.

③ The alcohol extract of Rhodiola rhodiola after reflux extraction is transferred to a reduced-pressure distillation device, the distillation temperature is maintained at about 60℃, and the alcohol is volatilized and removed from the alcohol extract of Rhodiola chrysanthemum under reduced pressure.

④ Place the non-woven bag containing the extract from which the alcohol has been removed and the rhodiola powder that has undergone reflux extraction back into the hot water extraction tank, and add water at a volume ratio of 3 to 6 times the dry weight (l/kg) of the rhodiola as the extraction solvent. A hot water extract of rhodiola is obtained by performing hot water extraction under conditions of an extraction temperature between 85°C and 95°C and an extraction time within the range of 6 to 12 hours.

⑤ In order to remove the Rhodiola defolia from the Rhodiola extract after the hot water extraction process, the temperature in the extraction tank is cooled to below 60°C and then transferred to a storage tank for purification of the Rhodiola extract. Prepare a Rhodiola rosea complex extract for purification by mixing with .

⑥ In order to remove heavy metals and residual pesticide components in the rhodiola extract obtained in ⑤, the rhodiola extract is passed through an activated carbon filter device.

⑦ In order to remove insoluble particles and carbohydrates remaining in the rhodiola extract that has passed through the activated carbon filter, the complex extract is cooled to 5℃ and passed through an ultra-high-speed centrifuge (rotating speed; 15,000-20,000 R.P.M.) to remove insoluble particles due to centrifugal force. Obtain a colored, transparent, purified Rhodiola rosea complex extract from which particulate matter and insoluble carbohydrate components have been removed.

⑧ Food that is permeated by passing through a TFF-type ultra-filtering system equipped with a 0.45㎛ sterilizing membrane to remove bacterial microorganisms and inert substances existing in a colloidal state in the rhodiola extract complex that has passed through an ultra-high-speed centrifuge. Obtain the final purified Rhodiola rosea complex extract that ensures stability.

⑨ The final purified Rhodiola rosea complex extract is put into a vacuum concentrator and concentrated under reduced pressure conditions with a concentration temperature of 80°C or lower until the sugar meter reading reaches 65 brix to obtain a rhodiola complex extract concentrate, or the reduced pressure concentration is concentrated to about 10 brix as measured by the sugar meter. Afterwards, the Rhodiola extract complex is placed in a freeze dryer, frozen at ultra-low temperature to -60℃, and then dried in vacuum while slowly raising the temperature to 35℃ for about 48 hours to obtain a dried powder of Rhodiola extract complex.

[Preparation Example 3] Preparation of root bark extract

① Cut the dried root bark into powder or pieces of about 2 to 5 mm, put the rhodiola root powder (pieces) into a non-woven bag, and then put it in a hot water extraction tank to obtain a ratio of 3 to 6, which is the dry weight (ℓ/kg) of the root bark. A hot water extract of Rhizoma root bark is obtained by adding about twice the volume ratio of water as an extraction solvent and performing hot water extraction under conditions of an extraction temperature of 85°C to 95°C and an extraction time within the range of 6 to 12 hours.

② In order to remove the radicle bark from the radicle bark hot water extract after the hot water extraction process, the temperature in the extraction tank is cooled to below 60℃ and then transferred to a storage tank for purification of the radicle root bark hot water extract. Heavy metals and residual pesticide components in the radicle bark hot water extract are removed. In order to remove the root bark, the hot water extract is passed through an activated carbon filter device.

③ In order to remove insoluble particulates and carbohydrates remaining in the hydrothermal extract of Radix root bark that has passed through the activated carbon filter, the complex extract is cooled to 5℃ and passed through an ultra-high-speed centrifuge (rotating speed; 15,000-20,000 R.P.M.) to remove insolubility due to centrifugal force. Obtain a colored, transparent, purified hot water extract of Euderm bark with particulate matter and insoluble carbohydrate components removed.

④ Food that is permeated by passing through a TFF-type ultra-filtering system equipped with a 0.45㎛ sterilizing membrane to remove bacterial microorganisms and inert substances existing in a colloidal state in the Rhodiola rosea complex extract that has passed through an ultra-high-speed centrifuge. Obtain a final purified hydrothermal extract of Radix root bark that ensures stability.

⑤ The final purified radicle bark hot water extract is put into a reduced pressure concentrator and concentrated until the dry weight is about 10% under reduced pressure conditions with a concentration temperature of 80℃ or less. Then, the radicle bark hot water extract is put into a freeze dryer and ultra low temperature down to -60℃. After freezing, the temperature is slowly raised to 35°C for about 48 hours and dried in a vacuum to obtain a dry powder derived from hot water extraction of the root bark.

[Preparation Example 4] Preparation of trillium and coltsfoot complex extract

① Mix the dried leaves of Sambaekcho and the leaves, stems, and roots of butterbur with a weight ratio of 1:1, then cut them into powders or pieces of about 2~5mm, and then mix the powders of Sambaekcho and butterbur with them. Put it in a non-woven bag and put it into a circulating reflux extraction tank.

② Add 30 to 70% alcohol as an extraction solvent to the circulation-type reflux extraction tank to submerge the non-woven bag containing the butterbur and butterbur, and then extract for 12 hours while refluxing the solvent at an extraction temperature not exceeding 40°C. .

③ The reflux-extracted alcoholic extracts of Trisodium vulgaris and butterbur are transferred to a reduced-pressure distillation device, maintaining the distillation temperature at about 60°C, and the alcohol is volatilized and removed from the mixed alcohol-alcohol extracts of Trisodium vulgaris and butterbur under reduced pressure.

④ Place the extract from which the alcohol has been removed and the non-woven bag containing the reflux extract and butterbur powder back into the hot water extraction tank and add water at a volume ratio of 3 to 6 times the dry weight (ℓ/kg) of the ginseng root and butterbur. It is added as an extraction solvent and subjected to hot water extraction under conditions of an extraction temperature of 85°C to 95°C and an extraction time within the range of 6 to 12 hours to obtain a mixed hot water extract of Trifolium primrose and butterbur.

⑤ In order to remove the butterbur and butterbur extract from the mixed hot water extract of Trifolium vulgaris and butterbur after the hot water extraction process, the temperature in the extraction tank is cooled to below 60℃ and then transferred to a storage tank for purification of the mixed hot water extract of Tribal celery and butterbur. By mixing the mixed alcohol extract of Trifolium vulgaris and Butterbur in item ③ above with the hot water extract, a composite extract of Tribal secundum and Butterbur for purification is prepared.

⑥ In order to remove heavy metals and residual pesticide components in the Trifolium vulgaris and butterbur complex extracts obtained in paragraph ⑤, pass the Trillium vulgaris and butterbur complex extracts through an activated carbon filter device.

⑦ In order to remove insoluble particulates and carbohydrates remaining in the trifoliate and butterbur complex extract that passed through the activated carbon filter, the complex extract is cooled to 5℃ and then passed through an ultra-high-speed centrifuge (rotating speed; 15,000~20,000 R.P.M.). Obtain a colored, transparent and purified complex extract of Trifolium prickly pear and butterbur, from which insoluble particulate matter and insoluble carbohydrate components are removed.

⑧ In order to remove bacterial microorganisms and inert substances existing in a colloidal state in the complex extract of Trifolium prickly pear and butterbur that has passed through an ultra-high-speed centrifuge, it is permeated by passing it through a TFF-type ultra-filtering system equipped with a 0.45㎛ sterilizing membrane. Obtain the final purified trifoliate and butterbur complex extract that ensures food safety.

⑨ The final purified trifolia and butterbur complex extracts are placed in a reduced pressure concentrator and concentrated until the dry weight is about 10% under reduced pressure conditions with a concentration temperature of 80°C or lower. Then, the trillium and butterbur complex extracts are placed in a freeze dryer and stored at -60°C. After freezing at very low temperatures, slowly raising the temperature to 35℃ for about 48 hours and drying in a vacuum, you can obtain a dried powder of a complex extract of trifoliate and butterbur.

[Experimental example]

[Experimental Example 1] Analysis of glucose content in ginseng extract

Since approximately 95% of polysaccharide components, including acidic polysaccharides derived from ginseng, are composed of glucose, the glucose content was analyzed to determine the polysaccharide content of ginseng extract (Panaxana Ginsan).

1. Weigh exactly 2g of ginseng extract powder sample, place it in an Erlenmeyer flask, and add 50ml of distilled water to completely dissolve.

2. Add 4 ml of concentrated Hcl (36%) to the completely dissolved sample.

3. For acid decomposition, place an Erlenmeyer flask in a reflux condenser with a sealed top and heat in a water bath at 80°C for 3 hours.

4. When acid decomposition is completed, leave to cool with the mouth of the Erlenmeyer flask blocked, then add 4 ml of 36% NaOH and add a small amount of 1N-NaOH to neutralize it to around pH 7.

5. Put the sample in a 100 ㎖ mass flask and fill it with distilled water up to the 100 ㎖ mark. (Extraction solvent dilution factor in the calculation formula: 100 ㎖)

6. Insert a folded filter paper into a triangular funnel and filter the sample to remove insoluble substances.

7. Dilute the filtered sample according to the dilution factor (x2, x4, x8, x16, x32, x64, x128) specified in the test tube.

8. Add 1 ml of each diluted sample to another test tube and add 1 ml of D.N.S (Dinitro salicylic acid) reagent.

9. Heat the test tubes containing the D.N.S reagent in boiling water for 5 minutes, then leave to cool. Add 8 ml of distilled water to each test tube and mix well with a Voltex mixer.

10. Set the measurement wavelength of the spectrophotometer to 540 nm, measure the measurement by inserting a quartz cell containing distilled water, adjust the set reference value (O.D = 0), then place the sample in the quartz cell and measure the absorbance.

11. The O.D value according to the dilution factor of the sample is shown in Table 1 below.

Dilution factor X 2 X 4 X 8 X 16 X 32 X 64 X 128 O.D value 2.342 1.617 0.905 0.653 0.338 0.184 0.089

12. In order to convert the measured O.D value into a sample concentration value, the above operation is repeated using a glucose standard product to measure the absorbance value for each glucose concentration to obtain a glucose standard sample concentration value. Confirm the calculation formula.

13. The O.D value according to the dilution factor of the sample is calculated as the sample concentration value based on glucose, as shown in the table below.

(Calculation formula) Sample concentration value = (O.D value + 0.0141)/0.0007

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 3365.9 2330.1 1313.0 953.0 503.0 283.0 147.3

14. Calculate the glucose content of the analyzed sample using the formula below using the sample concentration value, dilution factor, and extraction solvent dilution factor value.

<Equation>

Dilution factor X 2 X 4 X 8 X 16 X 32 X 64 X 128 Glucose content (%) 33.7 46.6 52.5 76.2 80.5 90.6 94.3 Glucose content average value (5); Excluding dilution factor x2 and x128 69.3

As a result, it was confirmed that the ginseng extract of the present invention contains about 69.3% glucose.

[Experimental Example 2] Analysis of molecular weight distribution of polysaccharides in ginseng extract

1. Accurately weigh 50 mg of ginseng extract powder sample, place in a test tube, add 5 ml of distilled water, and completely dissolve using a Voltex mixer.

2. Put the dissolved sample into the syringe and pass it through a 0.45㎛ nylon syringe filter to complete the preparation of the sample for GPC analysis.

<Configuration of analysis equipment>

1. Pump: Shimadzu LC-20AD

2. Injector: Shimadzu SIL-20A

3. Column Oven: Shimadzu CTO-20A

4. Column: PSS Suprema 10000Å + 3000Å + 30Å

5.Detector: ELSD

<Analysis conditions>

1. Flow Rate: 1.0 ㎖/min

2. Injection Volume: 50㎕

3. Oven Temperature: 40℃

4. Analysis Time: 40 ~ 50min

<Finding Calibration Curve and Function>

1. Use PSS Dextrane 1,400,000 333,000 164,000 35,600 8,100 908 as a standard sample and prepare it in the same way as the analysis sample above.

2. Calibration Curve

[Figure 1]

3.Function

f(x)=7.655291e-004*X^3-3.181748e-002*X^2+0.1386024*X+6.373336(X=x-T.LIMIT) R^2=0.9999212 Dispersion=0.0093897 T.LIMIT=15min

4. Calibration Table

The results are as shown in Table 5 below.

# Time(min) Molecular Weight Error(%) Active One 21.452 1400000 -0.6085 Enabled 2 24.375 333000 3.4946 Enabled 3 25.431 164000 -2.5961 Enabled 4 27.732 35600 -2.0459 Enabled 5 29.925 8100 2.0257 Enabled 6 33.246 908 -0.4096 Enabled

Peak# Ret. Time Weight Average Molecular Weight Area(%) One 19.98 2,243,426 1.68 2 20.35 1,726,284 0.87 3 24.87 562,356 4.25 4 25.52 181,813 19.43 5 27.64 40,592 17.08 6 30.15 6,308 8.54 7 32.07 3,555 12.36 8 34.96 536 35.34 9 37.88 107 0.45 Area(%) Total 100.00 Peak number (Mw 2,000,000 ~ Mw3,500) 6 Area(%) 62.53

[Experimental Example 3] Analysis of sugar composition of ginseng polysaccharide in ginseng extract

1. From the above GPC analysis results, 30 ml of each ginseng polysaccharide component present at retention times of 20 minutes, 25 minutes, and 27 minutes was collected in a test tube and powdered in a freeze dryer.

2. 1 g of ginseng polysaccharide powder sample was dissolved in 10 ml of distilled water, 12 ml of 60% perchloric acid was added, and the sample was extracted and hydrolyzed in boiling water for 4 hours.

3. The hydrolyzed ginseng polysaccharide solution was neutralized with NaOH solution, 40 ml of ethanol was added to 10 ml of the decomposition solution, centrifuged at 5,000 r.p.m for 20 minutes, and the supernatant was collected.

4. The supernatant was concentrated at 60°C to remove ethanol, dissolved in distilled water, filtered through a Millipore filter (pore size 0.45㎛), and analyzed using HPLC (High Performance Liquid Chromatography).

5. D-glucose, L-arabinose, D-galactose, xylose, and L-ramrose from Merck were used as sugar standards, and the HPLC analysis conditions are as shown in the table below.

Instrument Waters HPLC/ALC-244 Flow rate 1.0㎖/min Packing material Lichrosorb NH ₂ (Merck) Chart speed 0.5㎝/min Column 4.6(i.d.) Detector RI, 8X Mobile phase Acetonitrile/H ₂ O (80/20)

The results are shown in Tables 7 to 9 below.

[HPLC analysis results of ginseng polysaccharide with molecular weight of 1,726,284]

Type per composition Retention Time (minutes) content(%) glucose 2.7 90.52 galactose 3.5 9.15 Arabinose 3.8 0.04 rhamnose 4.3 0.01 xylose 4.9 0.16

[HPLC analysis results of ginseng polysaccharide with molecular weight of 181,813]

Type per composition Retention Time (minutes) content(%) glucose 3.1 91.82 galactose 3.3 2.48 Arabinose 3.8 0.52 rhamnose 4.6 4.60 xylose - 0

[HPLC analysis results of ginseng polysaccharide with molecular weight of 40,592]

Type per composition Retention Time (minutes) content(%) glucose 3.1 79.51 galactose 3.4 4.08 Arabinose 3.8 11.42 rhamnose 4.8 3.81 xylose - 0

6. In addition, in order to verify the results of HPLC analysis of the three components of the separated ginseng polysaccharides, the glucose content, a representative sugar, was measured using the above glucose content analysis method.

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 1,726,284]

dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 5.565 2.915 1.505 0.767 0.395 0.196 0.094

* Number of grams of sample: 2g

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 7970.0 4185.0 2170.0 1116.3 584.4 300.6 154.1

* Calculation formula: Sample concentration value = (O.D value +0.0141)/0.0007

Dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 79.7 83.7 86.8 89.3 93.5 96.2 98.6 Excluding dilution factor x2 and x128 89.9

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 181,813]

Dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 5.719 2.982 1.529 0.780 0.403 0.199 0.095

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 8190.0 4280.0 2205.0 1133.8 596.3 304.1 155.5

dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 81.9 85.6 88.2 90.7 95.4 97.3 99.5 Excluding dilution factor x2 and x128 91.4

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 40,592]

dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 4.907 2.604 1.344 0.679 0.342 0.171 0.082

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 7030.0 3740.0 1940.0 990.0 509.4 264.7 137.2

dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 70.3 74.8 77.6 79.2 81.5 84.7 87.8 Excluding dilution factor x2 and x128 79.6

As a result, the results of the glucose content analysis method and the HPLC analysis method showed similar results, ensuring the reliability of the sugar content analysis value by the HPLC analysis method.

[Experimental Example 4] Analysis and evaluation of ginseng saponin content of ginseng extract (Panaxana Ginsan)

1. Weigh exactly 1g of ginseng extract powder sample and place it in an Erlenmeyer flask, then add 60ml of distilled water and completely dissolve.

2. Put 60 ml sample into 250 ml separator reed, add 60 ml ether, shake several times to extract lipids, then separate layers.

3. When the separation of the ether layer and the sample layer in the separatory funnel is completed, the ether layer is removed, then 60 ml of saturated butanol is added and the saponin is extracted by shaking several times.

4. When the separation of the saturated butanol layer and the sample layer in the separatory funnel is completed, the upper saturated butanol layer is recovered and placed in a 250 ml Erlenmeyer flask, and the sample is placed back into the separatory funnel.

5. Repeat experimental operations 3 and 4 a total of 3 times and collect 180 ml of saturated butanol layer in a 250 ml Erlenmeyer flask.

6. Discard the sample for which saponin extraction has been completed, add 180 mL of saturated butanol layer back into the separation reed, and then add 50 mL of distilled water to wash the saturated butanol layer.

7. Place 180 ml of the washed saturated butanol layer into a weighed Erlenmeyer flask, attach the Erlenmeyer flask to an evaporator, immerse it in a water bath at 80°C, and concentrate under reduced pressure.

8. Place the Erlenmeyer flask with completed reduced pressure concentration in a dry oven at 105°C, dry for about 15 minutes, cool in the desiccator, and then re-measure the weight of the Erlenmeyer flask containing roughasponin.

<Equation>

Erlenmeyer flask weight after concentration (g) 126.6877 Empty Erlenmeyer flask weight (g) 126.5230 Ginseng saponin (%) 16.47 Ginseng saponin (mg/g) 164.7

[Experimental Example 5] Analysis and evaluation of ginseng components (Ginsenosides) of ginseng extract (Panaxana Ginsan)

1. Ginsenoside standard substances (Rb1, Rg1, Re, Rd, Rc, Rb2) were weighed at 50 mg each in a 50 mL volumetric flask, and then diluted with 70% methanol to prepare a standard stock solution (1 mg/mL). Make a standard solution by appropriately diluting the stock solution with 70% methanol.

2. Take 5g of ginseng extract powder sample, dilute with water to 50ml, centrifuge, activate by successively passing 5ml of methanol and 10ml of distilled water through C18 Cartridge for purification, and adsorb 5ml of centrifuged sample solution. I order it.

3. Wash the adsorbed C18 Cartridge with 25 ml of distilled water and 10 ml of 25% methanol (2~3 ml per minute), elute with 4 ml of methanol, collect in a 5 ml flask, and dilute to the indicated line with methanol, which is used as a test solution. .

4. In addition, in order to compare the ginseng ingredient content of Panaxena Jinsan, a ginseng extract of the present invention, with the ginseng ingredient content of a red ginseng concentrate from another company already sold as a product, 5 ml of a sample of a red ginseng concentrate from another company was taken and diluted with water to make 20 ml. A comparative test solution was prepared using the same method described in paragraphs 2 and 3.

5. The detection wavelength of the UV absorbance detector is set to 203㎚ and the octadecylsilica column is

Using the equipped HPLC, the mixing ratio of the mobile phase water and Acetonitrile was varied for each time period to analyze the content of each type of Ginsenoside (Rb1, Rg1, Re, Rd, Rc, Rb2) in the ginseng extract Panaxena Jinsan. The result table and comparison graph are shown. Same as Figure 1. Since the Ministry of Food and Drug Safety Notification standard for ginseng notification-type health functional food ingredient is that the sum of the contents of Ginsenoside Rg1 and Rb1 is 3 to 80 mg, looking at the result table below, the sum of the contents of Ginsenoside Rg1 and Rb1 of Panaxena Jinsan is 26.96 mg. It is judged to meet the quality standards for ginseng notification type health functional food ingredients.

Ginsenoside types Content (mg/g) Ginsenoside types Content (mg/g) Ginsenoside Rg1 8.33 Ginsenoside Re 13.32 Ginsenoside Rb1 18.63 Ginsenoside Rc 14.94 Ginsenoside Rb2 13.97 Ginsenoside Road 6.01

[Experimental Example 6] Protein content analysis evaluation of ginseng extract (Panaxana Ginsan) (crude protein analysis method - Semi micro Kjeldahl method)

1. Measure 2g of ginseng polysaccharide extract powder sample and place it in a Kjeldahl flask. Wrap the sample in parchment paper and place it in a Kjeldahl flask.

2. Add 3g of decomposition accelerator, 0.25g of titanium dioxide, and 25ml of H2SO4 (sulfuric acid) and heat.

Decomposition accelerator: 50g K2SO4 (potassium sulfate), 5g CuSO4 (copper sulfate) => Grinding => Mixing

3. Due to heating, the color of the sample solution changes from black brown to dark brown, and finally changes from blue to yellow green to transparent color. From this point, heat for another hour to complete decomposition and then cool for 30 to 40 minutes.

4. Transfer the sample to a 500 ml Erlenmeyer flask, rinse the Kjeldahl flask with distilled water, and add it to the Erlenmeyer flask to make the final volume 200 ml.

5. Take 40 ml of the 200 ml of diluted sample solution, place it in a Kjeldahl flask, and add 15 ml of NaOH for neutralization.

6. Take 25 ml of 4% H3BO3 in a 300 ml beaker and add about 3 drops of mixing indicator.

The end of the cooling pipe should be submerged in 4% H3BO3 to prevent NH4 (ammonia) from escaping due to heating.

7. Turn on the coolant and heat it for a long time. The color of H3BO3 with the addition of indicator changes from pink to light green.

8. Stop heating when the total amount of NH4 that passed through the cooling pipe and the total amount of H3BO3 in the beaker reaches 50 ml.

9. Titrate with 0.05-N HCl and calculate according to the formula.

<Equation>

V1: 0.05N-HCl ㎖ consumed in the sample, V0: 0.05N-HCl ㎖ consumed in the blank test,

F: 0.05N-HCl Factor (1.033), 14: Nitrogen conversion factor, D: Dilution factor of sample and distilled water (constant volume/collection amount) = 5, S: Sample collection amount (g)

Types of Ginseng Polysaccharides Protein content (%) Ginseng polysaccharide with a molecular weight of 1,726,284 1.5 Ginseng polysaccharide with a molecular weight of 181,813 1.2 Ginseng polysaccharide with a molecular weight of 40,592 0.8

Below is a table summarizing the results of the above experiments.

Analysis items Analysis method Result value Glucose content D.N.S. Law 69.3% Molecular weight distribution G P C method Peak number 6 (Mw 2,000,000 ~ Mw3,500) area 62.53% (Mw 2,000,000 ~ Mw3,500) Constituent sugars H P L C method Mw(1,726,284) glucose 90.52% galactose 9.15% Arabinose 0.04% rhamnose 0.01% xylose 0.16% Mw(181,813) glucose 91.82% galactose 2.48% Arabinose 0.52% rhamnose 4.60% xylose - Mw(40,592) glucose 79.51% galactose 4.08% Arabinose 11.42% rhamnose 3.81% xylose - Ginseng Saponins Saturated butanol method 164.7(mg/g) Ginseng ingredients H P L C method Ginsenoside Rg1+Rb1 26.96㎎ protein Semi micro Kjeldahl method Mw(1,726,284) 1.5% Mw(181,813) 1.2% Mw(40,592) 0.8%

[Experimental Example 7] Vascular relaxation, blood pressure lowering, and antioxidant test of Panaxena ginsan, Rhodiola rosea, Rhodiola root bark, Triacium vulgaris, and butterbur complex extracts

Preparation Example 1, Preparation Example 2, Preparation Example 3. Panaxena ginsan extract, rhodiola root extract, radix root bark extract, and Triacium vulgaris and coltsfoot complex extract prepared in Preparation Example 4 were mixed and extracted at a weight mixing ratio as shown in the table below. Experiments were conducted on the mixture's vascular relaxation, blood pressure lowering, and antioxidant effects.

Extract name Panaxena Jinsan rhodiola Root skin Three hundred seconds, butterbur Weight mixing ratio 20% 40% 20% 20%

A. Vasorelaxation test

① Forty 15-week-old male rats with hypertensive disease were administered a daily dose of 10, 30, or 100 mg of an extract mixture (30% of dry weight) of Panaxena ginsan extract, Rhodiola rosea extract, Rhythmia root bark extract, Trifolium primrose, and butterbur complex extract. It was administered orally using a syringe at a dose of /kg, and each animal was divided into 10 experimental groups and 10 control groups according to the administered dose and reared for 14 days.

② The endothelial tissue of the thoracic aorta of bred male rats was immersed in Kreb's Ringer bicarbonate buffer solution, and the aorta was tightened by cumulative concentration with 0.3 μM phenylephrine solution as shown in the figure, and then measured while applying a tensile force of 2 g.

③ As can be seen in Figure 5, the experimental group that consumed a daily dose of 100 mg/kg of the extract mixture with a dry weight of 30% showed the greatest vascular relaxant effect and was judged to have the best elasticity of blood vessels. Therefore, the extract mixture is considered to be good for blood vessel health. It has been confirmed to have excellent effects.

B. Blood pressure lowering test

① The daily intake of the extract mixture (30% dry weight), which showed the best vascular relaxation effect, was determined to be 100 mg/kg, and 10 12-week-old male rats with hypertension were treated as an experimental group and administered a daily dose of Captopril, a blood pressure medication. 10 male rats treated with 30 mg/kg were used as control 1, 10 male rats without any treatment were used as control 2, and 10 male rats of the same age with normal blood pressure were used as control 3, and were orally administered via syringe for 42 days. It was bred in this way.

② The experiment was conducted by measuring blood pressure and heart rate once a day using the tail-cuff method.

③ As can be seen in Figure 6, in the experimental group that consumed a daily dose of 100 mg/kg of the extract mixture with a dry weight of 30%, symptoms of high blood pressure were significantly improved, compared to control group 1, who took blood pressure medication, and control group 3, who had normal blood pressure. Similar blood pressure levels were maintained, and there was little significant change in heart rate when compared to the experimental group and control group 1, 2, and 3. Therefore, it was confirmed that these extract mixtures were effective in significantly improving hypertension symptoms.

C. Antioxidant test

In the case of hypertensive disease, free radicals increase and excessive amounts of superoxide, hydrogen peroxide, and lipid peroxide in the plasma can cause damage to blood vessels. Therefore, MDA was removed from the liver tissue of the experimental and control groups 1, 2, and 3 used in the blood pressure lowering experiment. As a result of measuring the degree of antioxidant by measuring the amount of malondialdehyde and LDH (lactate dehydrogenase) in plasma, the experimental group showed results closer to Control 1 and Control 3 than Control 2, confirming the antioxidant effect of these extraction mixtures ( Figure 7).

[Experimental Example 8] Testing the sexual function improvement effect of Panaxena ginsan, Rhodiola rosea, Rhodiola root bark, Triacium vulgaris, and butterbur complex extracts

A. Manufacturing test drugs for human application experiments

① For a certain amount of intake by the experimental group, the extract mixture powder of freeze-dried Panaxena ginsan extract, Rhodiola rosea extract, Yugeun bark extract, Triacium chinensis and coltsfoot complex extract, and starch were mixed in a ratio of 1:1 in the same weight ratio as used in Experimental Example 7. It was mixed and filled into hard capsules with a capacity of 300 mg so that each capsule could equally contain 150 mg of the extract mixture powder.

② In order to ingest a certain amount of the control group, caramel coloring and synthetic flavoring with a similar color to the extract mixture powder were mixed with starch in an appropriate ratio and filled into a 300㎎ hard capsule, so that it had the same color and shape as the capsule containing the extraction mixture powder. A placebo containing was prepared.

B. Method for evaluating sexual function enhancement effects through human application experiments

① The subjects of the human application experiment are men over the age of 20 who are clinically judged to have decreased sexual function through a medical examination and questionnaire by a specialist. They are physically healthy, free from lesions such as high blood pressure, diabetes, or neuropsychiatric disease, and have regular sexual relations. 20 people who could try were selected and divided into two groups, 10 of whom were the experimental group and the remaining 10 were the control group.

② Internationally recognized sexual function test for experimental and control groups before human application experiment

After distributing the IIEF (International Index of Erectile Function) questionnaire, participants were asked to fill out the answers and submit them.

③ The human application experiment period was set at 1 month, and the experimental group was instructed to take 2 capsules containing the extract mixture powder every day, morning and evening. The intake of the extract mixture powder was equalized to 600 mg per day for 30 days. The control group was instructed to take the same type of placebo as the experimental group, taking 2 capsules in the morning and evening for 30 days.

④ At the end of the human application experiment, the IIEF, a sexual function test questionnaire, was distributed to the experimental and control groups in the same manner as before, and they were asked to fill out the answers and submit the questionnaire.

C. Sexual function test evaluation and analysis method using IIEF

① IIEF, an internationally recognized sexual function test questionnaire, consists of five areas: erectile function, orgasm, sexual desire, sexual intercourse satisfaction, and sexual life satisfaction. The answers to a total of 15 questions are scored from 0 to 5, and the total score is It is evaluated as 5~15 (severe), 16~25 (moderate), 26~40 (mild), 41~55 (slight), and 56~75 (normality). .

② Judgment of the effect of improving sexual function through IIEF is that if there is an increase of 3 points or more when evaluating each item, an increase of 15 points or more when totaling all items, and the proportion of patients who improved is statistically significant, the test drug is analyzed to have an effect of improving sexual function. do.

D. Test results for male sexual function enhancement effect of extract mixture

① Table of IIEF questionnaire evaluation results for the experimental group before and after the human application experiment

division Evaluation items and scores total score Judgment erectile function orgasm sexual desire sexual intercourse
satisfaction sex life
satisfaction Experiment 1 Before 6 5 9 7 8 35 Mild After 12 11 13 12 12 60 Normality Experiment 2 Before 10 8 12 9 9 48 Slight After 14 13 15 13 14 69 Normality Experiment 3 Before 7 9 10 8 8 42 Slight After 11 13 13 14 15 66 Normality Experiment 4 Before 8 7 8 7 9 39 Mild After 11 12 11 13 12 59 Normality Experiment 5 Before 5 7 7 6 6 31 Mild After 9 10 12 9 10 50 Slight Experiment 6 Before 12 9 10 10 9 50 Slight After 14 12 12 13 13 64 Normality Experiment 7 Before 9 8 11 9 10 47 Slight After 11 11 13 12 12 59 Normality Experiment 8 Before 11 8 9 8 10 46 Slight After 13 12 12 13 13 63 Normality experiment 9 Before 4 5 8 6 5 28 Mild After 8 8 10 10 9 45 Slight Experiment 10 Before 8 9 9 7 8 41 Slight After 10 12 12 11 10 55 Slight

② Table of IIEF questionnaire evaluation results for the control group before and after the human application experiment

division Evaluation items and scores total score Judgment erectile function orgasm sexual desire sexual intercourse
satisfaction sex life
satisfaction contrast 1 Before 9 9 12 10 11 51 Slight After 11 10 12 11 11 55 Slight contrast 2 Before 7 7 8 10 10 42 Slight After 10 8 10 11 10 49 Slight contrast 3 Before 10 8 12 9 9 48 Slight After 9 9 12 8 8 46 Slight contrast 4 Before 9 9 9 8 8 43 Slight After 10 10 10 9 8 47 Slight contrast 5 Before 11 9 10 10 11 51 Slight After 13 11 12 12 12 60 Normality contrast 6 Before 6 8 8 7 7 36 Mild After 7 9 9 8 7 40 Mild contrast 7 Before 8 7 10 8 9 42 Slight After 9 9 9 8 10 45 Slight contrast 8 Before 5 8 7 7 8 35 Mild After 8 9 9 10 9 45 Slight contrast 9 Before 9 9 10 9 10 47 Slight After 11 10 11 10 10 52 Slight contrast 10 Before 12 10 12 9 9 52 Slight After 10 10 13 10 9 52 Slight

③ Analysis of IIEF survey results showing an increase of more than 3 points for each item

division Evaluation items increase number
(50 total) growth rate
(%) erectile function orgasm sexual desire sexual intercourse
satisfaction sex life
satisfaction control group 2 0 0 0 0 2 4 experimental group 6 10 8 10 8 42 84

As a result of the IIEF survey, 84% of the experimental group who took the extract mixture increased by more than 3 points in each evaluation item, while only 4% of the control group who took the placebo (Figure 8a).

④ As a result of the IIEF survey, the overall combined score increased by more than 15 points.

division Total total score increased by 15 points or more population Increase rate (%) control group 0 10 0 experimental group 7 10 70

As a result of the IIEF survey, the overall combined score of the control group that took the placebo did not increase by more than 15 points after the experiment compared to before the human application experiment, while the experimental group that took the extract mixture showed an increase rate of 70%, showing an effect of improving sexual function. It was found that

In conclusion, the experimental group that took the extract mixture at 600 mg per day for 30 days were people with mild or mild sexual dysfunction according to the IIEF survey before the human application experiment. As a result of taking the extract mixture, sexual function was improved and The enhancement effect increased by 84% in the evaluation of each item, and the final analysis showed that the experimental group that showed improvement in sexual function and enhancement effect compared to the control group increased by 70%. Panaxena ginsan extract, rhodiola extract, root bark extract, and complex extract of trifolium primrose and butterbur. The extract mixture is believed to have the effect of improving and enhancing male sexual function.

Claims (7)

a) Rhodiola extract;
b) butterbur extract; and
c) Contains one or more of ginseng extract and root bark extract as active ingredients,
A composition comprising one or more of the following uses:
i) improving vascular health or improving blood circulation;
ii) improving sexual function;
iii) improving motor skills; and
iv) Fatigue recovery. According to paragraph 1,
The composition further comprises a trifoliate extract. According to claim 1 or 2,
The extraction solvent for the extract contained in the composition is,
A composition comprising water, an organic solvent, or an aqueous solution thereof. According to paragraph 1,
A composition in which one or more of the above-mentioned sexual function improvement, exercise ability improvement, and fatigue recovery is achieved by improving vascular health or improving blood circulation. According to paragraph 1,
One or more of the rhodiola extract, butterbur extract, and ginseng extract and root bark extract,
A composition comprised in a weight ratio of 1:0.1 to 1:0.5 to 1.5. According to paragraph 2,
The trichome extract is,
A composition comprising 10 to 100 parts by weight based on 100 parts by weight of rhodiola extract. According to paragraph 1,
The composition is a food composition.