KR20240077489A - Specimen container in which cap and container body interfere during rotation of the cap - Google Patents

Abstract

A specimen container is provided that reduces the risk of worker exposure to hazardous substances and reduces the force required to cut the sealing sheet. The sample container includes a container body including a main body bottom, a main body side wall extending upward from the main body bottom, and a locking part protruding inward from the main body side wall; a container cap configured to form a connection with at least a side wall portion of the container body at an upper portion of the container body; and a filter bottom having an opening providing a filter function, and a locking protrusion protruding downward from the bottom of the filter, wherein the main body and the cap are coupled, and when the cap and the filter are coupled, the locking protrusion The lower surface is located lower than the upper surface of the locking portion.

Description

Specimen container in which the cap and the container body interfere during rotation of the cap {SPECIMEN CONTAINER IN WHICH CAP AND CONTAINER BODY INTERFERE DURING ROTATION OF THE CAP}

The present invention relates to a sample container, and more specifically, to a sample container containing physiological saline solution therein. More specifically, it relates to a specimen container that contains physiological saline solution inside, allows the specimen to be preserved with only a small amount of preservative solution, and maintains the preservative solution in a completely sealed state.

A smear test is a method of bacterial testing that involves smearing a sample on a glass slide, then staining the sample and observing it under a microscope. For example, a bacterial smear test can be performed by applying a thin layer of a sample such as cells or tissue collected from a human to a glass slide, selectively staining the sample, and then observing it under a microscope. Liquid-based cytology is a technique that replaces the conventional cytology test (Papanicolaou) by storing collected cell samples in a preservation solution and then placing the cell sample with a monolayer of preserved cells evenly dispersed on a slide and examining it. It was also developed. Liquid cytology can improve the specificity and sensitivity of the test by improving fixation of cells and minimizing unclear factors compared to conventional cytology tests.

This bacterial smear test has the advantage of quickly detecting and starting treatment for patients with active tuberculosis compared to other methods because it can quickly confirm infection with a simple stain for bacteriological diagnosis.

Meanwhile, cell tissue collected from the patient's lesion is transported to a location with testing equipment for accurate diagnosis of the disease. In this case, the movement of the collected cell tissue needs to be preserved for a fairly long time, so it is stored in a preservation solution (or fixative solution). The preservative solution maintains and preserves the state at the time of collection so that the cell tissue shed from the human body does not die, decompose, or change shape, and maintains a state suitable for observing or analyzing the structure of the cell tissue, or making specimens, etc. do. A mixed solution containing formaldehyde is usually used as a preservative solution.

Formaldehyde has a coagulating and polymerizing effect on proteins and is most commonly used as a preservative. However, formalin (formaldehyde solution) is not only highly toxic to the human body but is also volatile, so caution is required when handling it. Additionally, if volatilized formalin is left in the air for a long time, grade 1 carcinogens may be generated. If a person is exposed to gaseous formalin, formaldehyde can penetrate through the skin or mucous membranes. In particular, a high degree of caution is required when handling formalin because continuous inhalation of formaldehyde can cause central nervous system disorders or heart failure.

However, during the cell preservation process, testers such as clinicians or pathologists who perform tests are always exposed to formalin. Therefore, when handling formalin, the inspector wears a gas mask to minimize the hazard caused by formalin, but the worker's health is still exposed to harmful elements. For this reason, various attempts are being made to seal the preservation solution.

For example, Patent Document 1 (Korean Patent No. 10-1880052) discloses a sample storage container including a container body, a cutter member, a preservative solution receiving portion, a lid, and a temporary member. Additionally, Patent Document 2 (Korean Patent No. 10-1934819) discloses a sample container including a sample receiving portion and a cap portion. Patent Document 1 and Patent Document 2 disclose a structure in which the sealing film that seals the preservation liquid is cut by rotating the container body and lid.

For another example, Patent Document 3 (Korean Patent No. 10-1874304) discloses a human tissue preservation device including a storage housing, a screen net, a sorting member, a push rod, and an aluminum film. In addition, Patent Document 4 (Korean Patent No. 10-1736745) discloses a specimen container for tissue preservation including a cap portion, a housing, a slit portion, and a support jaw. Patent Document 3 and Patent Document 4 disclose a structure in which pressure is applied to the lid to cut the sealing film that seals the preservation liquid.

KR 10-1880052 B1, (Patent Document 2) KR 10-1934819 B1, (Patent Document 3) KR 10-1874304 B1 (Patent Document 4) KR 10-1736745 B1 (Patent Document 5) JP 2011-519587 A

The sample containers of the above-mentioned Patent Documents 1 to 4 are configured to seal the preservative solution using a sealing membrane, and to cut the sealing membrane through manipulation during use. However, there is a problem that significant force is required to cut the airtight membrane in Patent Documents 1 to 4. Some of the sample containers disclosed in the patent documents are on sale, but require considerable force, for example, about 3 atm, to cut the sealing membrane. In particular, many of the workers who perform this work in actual medical settings are female, and they are unable to incise the occlusive membrane with one hand, so they apply pressure with both hands, or drop the sample container while applying pressure, causing foaming in the preservative solution and air bubbles. Problems such as overflow are occurring.

Meanwhile, the process in which the examiner is exposed to formalin can occur not only in the process of putting a cell sample into a specimen container, but also in the process of manufacturing formalin suitable for cell fixation. In other words, workers may be exposed to formalin during the process of manufacturing or preparing a preservation solution with an appropriate formaldehyde concentration and containing other additional ingredients.

Accordingly, the problem to be solved by the present invention is to provide a sample container that can easily cut the sealing sheet and mix the preservative solution and the sample even if the operator applies relatively little force. At the same time, it provides a sample container that can further reduce the time workers are exposed to harmful substances such as formalin.

The problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the description below.

A sample container according to an embodiment of the present invention to solve the above problem includes a container body containing physiological saline solution in an internal space; A cap coupled to the top of the container body and containing a preservative solution sealed therein; and a cutting unit coupled to the lower part of the cap and including a cutting part protruding upward.

The preservative solution may be formalin containing 35.0% to 40.0% formaldehyde.

Here, the physiological saline solution and the preservative solution may be mixed to form a concentration of 8% to 12%.

In addition, when the container body and the cap are coupled, the physiological saline solution is configured to be completely sealed, and the coupling between the cap and the cutting unit may be a rotation-free coupling.

A sample container according to another embodiment of the present invention for solving the above problem includes a container body containing a first liquid in an internal space; A cap coupled to the top of the container body and containing a second liquid sealed therein, the cap further comprising a sealing sheet for sealing the second liquid; and a cutting unit coupled to the lower part of the cap and including a cutting part, wherein when an external force is applied to the cap, the cutting part cuts the sealing sheet from the bottom to the top, and the first liquid and the second It is configured to mix liquids.

A sample container according to another embodiment of the present invention for solving the above problem includes a cap including a sealing sheet and having a sealed first space therein; A container body reversibly coupled to a lower portion of the cap and having a second space, wherein the second space is closed when coupled to the cap and the second space is open when separated from the cap; A blocking ring disposed to be inserted between the cap and the container body when the cap and the container body are coupled; and a cutting unit that is reversibly coupled to the lower part of the cap and inserted into the container body while the cap and the container body are coupled.

The cap includes a ceiling portion, a fastening body portion protruding downward from the ceiling portion and having a first thread formed on an inner surface, a first side wall portion protruding downward from the ceiling portion and forming the first space, and the first side wall portion. It may include the sealing sheet disposed at the bottom to seal the first space.

In addition, the container body includes a first bottom, a second side wall portion that protrudes upward from the first bottom, has a second screw thread formed on an outer surface, and forms the second space, and an upper side from the first bottom. It may include a plurality of support parts that protrude from the outer surface of the second side wall and form a lower height than the second side wall part, and a ring support part that protrudes from the outer surface of the second side wall part and is located lower than the second screw thread.

The cutting unit includes a second bottom portion having a plurality of openings, a third side wall portion protruding upward from the second bottom portion, and a third side wall portion protruding upward from the second bottom portion and having a height lower than that of the third side wall portion. It may include an incision to form.

Furthermore, the cap and the container body may be coupled by screwing through the first screw thread and the second screw thread.

The cap and the cutting unit may be press-fitted through an outer surface of the first side wall portion and an inner surface of the third side wall portion.

Additionally, the blocking ring may be disposed between the lower surface of the fastening body portion and the upper surface of the ring support portion.

The cutting unit may further include a locking protrusion protruding downward from the second bottom portion.

Here, when the cap and the container body and the cap and the cutting unit are combined, the lower surface of the second bottom is in contact with the upper surface of the support part, and the lower surface of the locking protrusion is located lower than the upper surface of the support part. can do.

Additionally, the coupling between the cap and the cutting unit may be a rotation-free coupling.

The incision is a plurality of first incisions arranged in a circular manner, the first incision having a curved side and an upper end forming a tip, and a second incision surrounding the first incision from a plan view. The portion may include a second cutout portion that has a flat side and divides the space surrounded by the first cutout portion into a plurality of regions.

Additionally, the maximum height of the first incision may be greater than the maximum height of the second incision.

In some embodiments, when the cap is rotated by more than 90 degrees relative to the container body, the cap and the cutting unit may be configured to rotate together in a first angle range of less than 90 degrees.

Here, in a second angle range exceeding the first angle, the cap rotates, but the cutting unit may be configured to have rotation limited due to interference between the support portion and the locking jaw.

When the cap is rotated relative to the container body in a state in which the blocking ring is removed, the cap is screwed through the first screw thread and the second screw thread and is configured to move downward, and is press-fitted with the cap. The cutting unit is supported by the support portion and may be configured to be restricted from downward movement.

Here, as the cap moves downward, the separation distance between the sealing sheet and the cutout portion may be configured to gradually become closer.

Specific details of other embodiments are included in the detailed description. In addition, the effects according to the embodiments of the present invention are not limited to the contents exemplified above, and further various effects are included in the present specification.

Figure 1 is a perspective view of a sample container according to an embodiment of the present invention.
Figure 2 is an exploded perspective view of the sample container of Figure 1.
Figure 3 is a cross-sectional perspective view of the sample container of Figure 1.
Figure 4 is a perspective view of the cutting unit of Figure 1;
Figure 5 is a rear perspective view of the cutting unit of Figure 4.
Figures 6 to 11 are diagrams showing the process of using the sample container of Figure 1.

The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms, and only the embodiments serve to ensure that the disclosure of the present invention is complete, and those skilled in the art It is provided to fully inform the person of the scope of the invention, and the present invention is only defined by the scope of the claims.

That is, various changes may be made to the embodiments presented by the present invention. The embodiments described below are not intended to limit the embodiments, but should be understood to include all changes, equivalents, and substitutes therefor.

The terminology used herein is for describing embodiments and is not intended to limit the invention. As used herein, 'and/or' includes each and every combination of one or more of the mentioned items. Additionally, the singular form also includes the plural form unless specifically stated in the phrase.

The spatially relative terms 'below', 'beneath', 'lower', 'above', 'upper', etc. used in this specification are shown in the drawings. As such, it can be used to easily describe the correlation between one component or element and other components or elements. Spatially relative terms should be understood as terms that include not only the directions shown in the drawings, but also the different directions of the components when used. For example, when a component shown in a drawing is turned over, a component described as 'below or beneath' another component may be placed 'above' the other component. Accordingly, the exemplary term 'down' can be used to include both downward and upward directions.

As used herein, 'comprises' and/or 'comprising' do not exclude the presence or addition of one or more other components in addition to the mentioned components. The numerical range expressed using 'to' indicates a numerical range that includes the values written before and after it as the lower limit and upper limit, respectively. ‘About’ or ‘approximately’ means a value or numerical range within 20% of the value or numerical range stated thereafter.

For convenience of explanation, the first direction (X) refers to an arbitrary direction in a plane, and the second direction (Y) refers to a direction that intersects the first direction (X) in the plane. Additionally, the third direction (Z) refers to a direction perpendicular to the plane. However, it goes without saying that the matters stated in the claims are not limited thereto. Unless otherwise defined, 'plane' means the plane to which the first direction (X) and the second direction (Y) belong. In addition, 'plane perspective' refers to a viewpoint where the component represented in the drawing is viewed from one side in the third direction (Z) and the component is placed on the plane.

Hereinafter, the present invention will be described in detail with reference to the attached drawings.

Figure 1 is a perspective view of a sample container 10 according to an embodiment of the present invention. Figure 2 is an exploded perspective view of the sample container 10 of Figure 1. Figure 3 is a cross-sectional perspective view of the sample container 10 of Figure 1. Figure 4 is a perspective view of the cutting unit 301 of Figure 1. Figure 5 is a rear perspective view of the cutting unit 301 of Figure 4.

1 to 5, the sample container 10 according to this embodiment includes a cap 101 having a sealed first space (S1) and a container body 201 having an open second space (S2). Includes. Additionally, the sample container 10 may further include a blocking ring 401 and a cutting unit 301. The sample container 10 may be a container for performing biopsy or liquid based cytology, but the present invention is not limited thereto.

First, the cap 101 will be described. The cap 101 may have a sealed first space S1. A liquid substance (eg, first liquid F) may be accommodated in the first space S1. For example, the liquid material may include a preservative solution (F) containing harmful ingredients. The preservative solution (F) may be a solution containing a high concentration of formaldehyde.

In some embodiments, the lower limit of the formaldehyde concentration of the preservative solution (F) is about 25.0% (v/v), or about 26.0% (v/v), or about 27.0% (v/v), or about 28.0% ( v/v), or about 29.0% (v/v), or about 30.0% (v/v), or about 31.0% (v/v), or about 32.0% (v/v), or about 33.0% ( v/v), or about 34.0% (v/v), or about 35.0% (v/v).

The upper limit of the formaldehyde concentration of the preservative solution (F) is not particularly limited, but is, for example, about 50.0% (v/v), or about 49.0% (v/v), or about 48.0% (v/v), or about 47.0% (v/v), or about 46.0% (v/v), or about 45.0% (v/v), or about 44.0% (v/v), or about 43.0% (v/v), or about It may be 42.0% (v/v), or about 41.0% (v/v), or about 40.0% (v/v).

Specifically, the cap 101 protrudes downward from the upper ceiling portion 111 and the ceiling portion 111, and protrudes downward from the fastening body portion 121 and the ceiling portion 111 on which a first screw thread 121s is formed on the inner surface. It may include a first side wall portion 131 forming the first space S1, and may further include a sealing sheet 150 disposed at the bottom of the first side wall portion 131. The ceiling portion 111, the fastening body portion 121, and/or the first side wall portion 131 may be formed integrally with each other without physical boundaries.

The ceiling portion 111, the fastening body portion 121, and the first side wall portion 131 of the cap 101 may be made of synthetic resin or the like. For example, normal human grip strength, such as less than about 100 kgf, or less than about 90 kgf, or less than about 80 kgf, or less than about 70 kgf, or less than about 60 kgf, or less than about 50 kgf. It can have strength and rigidity without causing changes in appearance. In other words, the cap 101 is made of a material with high strength and rigidity that does not cause external deformation such as breaking, crushing, compressing, expanding, bending, bending, folding, or tearing due to normal external forces. It can be done with Examples of the material include polycarbonate-based resin, but the present invention is not limited thereto.

The ceiling portion 111 may be approximately circular when viewed from a plan view. The ceiling portion 111 may be a portion forming the outer upper surface of the cap 101. Additionally, the lower surface of the ceiling portion 111 may contribute to forming the first space S1.

The fastening body portion 121 may protrude downward from the outer peripheral edge of the ceiling portion 111. The fastening body portion 121 may have a substantially ring shape. In an exemplary embodiment, the fastening body portion 121 may be provided with a means for reversibly coupling to the container body 201, which will be described later, on its inner peripheral surface. For example, the coupling means may be a first screw thread (121s). The term 'reversible bond' used in this specification refers to a bond that can be repeatedly fastened and released between components without destruction or deformation of the components. When the cap 101 and the container body 201 are partially or completely coupled, the fastening body portion 121 may be located outside the container body 201. That is, the inner diameter of the fastening body portion 121 may be larger than the outer diameter of the container body 201, which will be described later.

The first side wall portion 131 may protrude downward from approximately the center side of the ceiling portion 111 . The first side wall portion 131 may have a substantially cylindrical shape with an open bottom. Additionally, from a plan view, the first side wall portion 131 may be surrounded by the fastening body portion 121. The protruding length of the first side wall portion 131 may be greater than the protruding length of the fastening body portion 121. When the cap 101 and the container body 201 are partially or completely coupled, the first side wall portion 131 may be inserted into the container body 201. That is, the outer diameter of the first side wall portion 131 may be smaller than the inner diameter of the container body 201, which will be described later. The first side wall portion 131 and the fastening body portion 121 are spaced apart from each other, and the lower surface of the ceiling portion 111 may be partially exposed between them.

In some embodiments, the outer surface of the first side wall portion 131 may be plain without threads, etc. Through this, it is possible to form a rotationally unrestricted coupling with the cutting unit 301, as will be described later. The term 'unrestricted rotation coupling' used in this specification refers to a coupling in which fastening and disengagement between components does not change through relative rotation between components, and at the same time, other components can freely rotate relative to one component. Examples of rotationally unrestricted couplings include clip couplings, interference fitting couplings (or fitting couplings), and the like. In addition, the first side wall portion 131 may form a coupling with the cutting unit 301, which will be described later, that can be freely moved in the vertical direction (i.e., in the third direction (Z)).

The sealing sheet 150 may have a substantially circular shape. The sealing sheet 150 may be placed or adhered to the bottom of the first side wall portion 131 to form a sealed first space S1. That is, the lower surface of the ceiling part 111, the inner surface of the first side wall part 131, and the upper surface of the sealing sheet 150 may form the first space S1 together.

The sealing sheet 150 may be made of a material that does not allow liquid substances to pass through, but may be made of a material that has weaker strength and rigidity than the ceiling portion 111 of the cap 101. For example, the sealing sheet 150 may be made of a metal thin film such as silver foil or a synthetic resin thin film that can be partially cut, torn, damaged, or opened by a typical external force.

Next, the container body 201 will be described. The container body 201 may have an open second space S2. A liquid substance (eg, second liquid W) may be accommodated in the second space S2. For example, the liquid material has a water content of about 95.0% (v/v) or more, or about 96.0% (v/v) or more, or about 97.0% (v/v) or more, or about 98.0% (v/v) or more. /v) or more, or about 99.0% (v/v) or more of liquid substances. Alternatively, it may contain pure water.

In an exemplary embodiment, the second liquid W may include physiological saline solution with a sodium chloride (NaCl) concentration of 1.0% or less, or about 0.9%. When physiological saline solution is used as the second liquid (W), tissue deformation, such as moisture flowing into the sample, can be minimized during the time after the initial sample is introduced and before it is mixed with the preservation solution (F) (first liquid). However, the present invention is not limited to this, and in another embodiment, the second liquid W may further contain additional components to the physiological saline solution, such as other components advantageous for preserving or fixing cells. That is, the second liquid (W) may be a solution in which 0.1% (v/v) to 1.0% (v/v) of sodium chloride is dissolved. Additionally, the volume of the second liquid (W) may be selected in consideration of the concentration and volume of the first liquid (F), but may be, for example, about 3 ml to 10 ml, or about 4 ml to 6 ml.

Additionally, the second space S2 may provide a space to accommodate samples such as cells, tissues, and cell tissues collected from a subject when the sample container 10 is used. That is, the second liquid W may be located in a space where the sample is accommodated. In general, during the cell collection process, an examiner such as a pathologist or clinician can grasp the specimen using a tool such as biopsy forceps or biopsy tweezers and insert it into the container body 201. At this time, specimens such as tissue collected immediately are generally viscous, so it is not easy to insert the specimen held by the tool into the container body 201. In particular, since the color of the tissue is usually light, white, or transparent, it is not easy for the examiner to confirm whether the tissue cells have been correctly introduced.

The sample container 10 according to this embodiment accommodates a liquid substance, that is, the second liquid (W), in the container body 201, and places the biopsy forceps holding the tissue cells in the second liquid (W) and stirs the tissue. It has the advantage of being able to easily introduce samples such as cells. Accordingly, there is no need to apply physical force in the process of separating the sample from the tool, and thus problems such as crushing or damage to the sample can be minimized.

When the container body 201 and the cap 101 are separated, the second space (S2) is an open space, but when the container body 201 and the cap 101 are combined, the second space (S2) can be completely sealed from the outside.

Specifically, the container body 201 has a first bottom 211 at the bottom, protrudes upward from the first bottom 211, has a second screw thread 221s formed on the outer surface, and forms a second space S2. It includes a second side wall portion 221 and one or more supports 251 protruding upward from the first bottom portion 211, and a ring support portion 221k disposed on the outer peripheral surface of the second side wall portion 221. More may be included. The first bottom portion 211, the second side wall portion 221, the support portion 251, and/or the ring support portion 221k may be formed integrally with each other without physical boundaries.

The first bottom part 211, the second side wall part 221, the support part 251, and the ring support part 221k of the container body 201 may be made of synthetic resin or the like. For example, a change in appearance occurs when applying a normal human grip strength, such as less than about 100 kgf, or less than about 90 kgf, or less than about 80 kgf, or less than about 70 kgf, or less than about 60 kgf, or less than about 50 kgf. It can have strength and rigidity that do not exist. That is, the container body 201 is made of a material with high strength and rigidity that does not cause external deformation such as breaking, crushing, compressing, expanding, bending, folding, or tearing due to normal external force. You can. The container body 201 may be made of the same or different material as the ceiling portion 111 of the cap 101 described above.

The first bottom portion 211 may be approximately circular in plan view. The first bottom 211 may be a portion that forms the bottom of the exterior of the container body 201. Additionally, the upper surface of the first bottom 211 may contribute to forming the second space S2.

The second side wall portion 221 may protrude upward from the first bottom portion 211 . The second side wall portion 221 may have a substantially cylindrical shape with an open top. The outer diameter of the second side wall portion 221 may be smaller than the inner diameter of the fastening body portion 121, and the inner diameter of the second side wall portion 221 may be larger than the outer diameter of the first side wall portion 131. Accordingly, the second side wall portion 221 may be partially inserted into the space between the above-described fastening body portion 121 and the first side wall portion 131. In some embodiments, when the cap 101 and the container body 201 are completely coupled, for example, fully screwed, the upper end of the second side wall portion 221 may contact the lower surface of the ceiling portion 111. .

In an exemplary embodiment, the container body 201 may be provided with a means for reversibly engaging the cap 101 described above on its outer peripheral surface. For example, the coupling means may be a second screw thread 221s. In order to use the sample container 10 according to this embodiment for liquid cell testing, the cap 101 and the container body 201 must be coupled and released multiple times. For example, one release and combination are required to introduce the sample into the second space (S2) of the container body 201, and another release and combination are required to take out the sample to smear the preserved sample. You can. The sample container 10 according to this embodiment adopts a screw coupling as a coupling between the cap 101 and the container body 201 to enable reversible coupling and easy coupling and disassembly.

Additionally, the first screw thread 121s and the second screw thread 221s may be configured to be screwed together at a predetermined angle. In order to form a complete connection between the cap 101 and the container body 201 and prevent leakage of the liquid contained therein, it is preferable that the screw tightening angle of the first screw thread 121s and the second screw thread 221s be large.

The sample container 10 according to this embodiment has a screw tightening angle of 270 when the first screw thread 121s and the second screw thread 221s are fully screwed with the blocking ring 401, which will be described later, removed. degrees or more, or about 280 degrees or more, or about 290 degrees or more, or about 300 degrees or more, or about 310 degrees or more, or about 320 degrees or more, or about 330 degrees or more, or about 340 degrees or more, or about 350 degrees or more , or may be combined at an angle of about 360 degrees or more. That is, when the separated cap 101 is placed on the top of the container body 201 and screwed together, it can be rotated at least three-quarters of a turn, and preferably at least one turn.

In addition, the upper limit of the screwing angle when the first screw thread (121s) and the second screw thread (221s) are completely screwed is about 400 degrees, or about 390 degrees, or about 380 degrees, or about 370 degrees, or about 360 degrees. degrees, or about 350 degrees. The screw tightening angle between the first screw thread 121s and the second screw thread 221s may be in a predetermined relationship with the locking protrusion 371 of the cutting unit 301, which will be described later. This will be described in detail later.

In some embodiments, in a state in which the blocking ring 401, which will be described later, is not removed, that is, in a state in which the second screw thread 221s and the blocking ring 401 are arranged to overlap, the first screw thread 121s and the second screw thread 121s When the screw thread 221s is fully screwed, the screw tightening angle may be about 60 degrees or more, or about 70 degrees or more, or about 80 degrees or more, or about 90 degrees or more.

The support portion 251 may protrude upward from the first bottom portion 211 . The support portion 251 may have a bar shape that protrudes upward from the first bottom portion 211. Additionally, the support portion 251 may be surrounded by the second side wall portion 221 when viewed from a plan view. The protruding length of the support portion 251 may be smaller than the protruding length of the second side wall portion 221. That is, the top of the support portion 251 may have a lower height than the top of the second side wall portion 221.

One or more support units 251 may be provided. For example, a total of four may be placed at 90-degree intervals. Three supports 251 are shown in the cross-sectional perspective view of FIG. 3, and the drawing illustrates a case where a total of four supports 251 (including parts not shown in the cross-sectional perspective view) are arranged. However, the present invention is not limited to this. no. In other embodiments, there may be only one support portion 251. However, at least one of the support portion 251 and the locking protrusion 371, which will be described later, may be plural.

The support portion 251 may function to form a step relative to the inner surface of the second side wall portion 221. During the coupling process between the cap 101 and the container body 201, it may function to support the lower surface of the cutting unit 301 in the direction of gravity (eg, the third direction (Z)). This will be described in detail later.

The ring support portion 221k may be located on the outer peripheral surface of the container body 201. The ring support portion 221k may have a substantially ring shape in plan view. The ring support portion 221k is located on the outer peripheral surface of the container body 201 like the second screw thread 221s, but may be located lower than the second screw thread 221s. The ring supporter 221k may function to support the blocking ring 401, which will be described later, in the direction of gravity (eg, the third direction (Z)).

The protruding length of the ring support portion 221k in the horizontal direction (e.g., the first direction (X), the second direction (Y), or the plane direction to which the first direction (X) and the second direction (Y) belong) is It may be greater than the degree of protrusion of the second screw thread 221s in the horizontal direction. That is, when the container body 201 is viewed from the bottom in the third direction (Z), the second screw thread 221s is not visible because it is hidden by the ring support portion 221k, and when viewed from the top in the third direction (Z), the second screw thread 221s is not visible. Both the screw thread 221s and the ring support 221k can be seen.

Next, the blocking ring 401 will be described. The blocking ring 401 may be a ring-shaped ring member. The blocking ring 401 may have a predetermined length in the third direction (Z). The blocking ring 401 may be configured to be inserted and placed between the cap 101 and the ring support portion 221k of the container body 201 in the initial state and then removed when the sample container 10 is used. The blocking ring 401 prevents coupling between the cap 101 and the container body 201 above a certain standard and prevents the cutting portion 351 of the cutting unit 301, which will be described later, from cutting the sealing sheet 150. It can perform the function. Specifically, the blocking ring 401 can prevent the cap 101 from moving downward beyond a predetermined standard based on the container body 201.

The inner diameter of the blocking ring 401 may be larger than the outer diameter of the second side wall portion 221 of the container body 201. More specifically, the inner diameter of the blocking ring 401 may be larger than the sum of twice the outer diameter of the second side wall portion 221 and the protruding length of the second screw thread 221s. In the initial state, the blocking ring 401 may be placed on the ring supporter 221k, and the container body 201 may be inserted into the blocking ring 401. For example, the upper end of the blocking ring 401 is in contact with the lower end of the fastening body portion 121 of the cap 101, and the lower end of the blocking ring 401 is the upper surface of the ring support portion 221k of the container body 201. It may be in contact with and disposed between the ring support portion 221k of the container body 201 and the fastening body portion 121 of the cap 101. In addition, the container body 201 and the cap 101 may be partially screwed together so that the distance between them in the third direction (Z) is fixed. Additionally, in the initial state, the blocking ring 401 may overlap the second screw thread 221s in the first direction (X) and the second direction (Y).

In an exemplary embodiment, the length of the blocking ring 401 in the third direction (Z) may be smaller than the length from the ring support portion 221k to the top of the second side wall portion 221. That is, when the blocking ring 401 is placed on the ring supporter 221k, the upper portion of the second screw thread 221s may be partially exposed. The blocking ring 401 may be made of the same or different materials as the cap 101 and/or the container body 201 described above.

Next, the cutting unit 301 will be described. The cutting unit 301 may be reversibly coupled to the lower portion of the cap 101, specifically, the lower portion of the first side wall portion 131. In a state where the cap 101 and the cutting unit 301 are coupled, and the cap 101 and the container body 201 are partially or completely coupled, the cutting unit 301 is inserted and placed into the container body 201. You can. The cutting unit 301 is between the cap 101 and the container body 201, specifically, the first liquid (F) contained in the first space (S1) is directed to the second liquid (W) in the second space (S2). It may be located in a flowing fluid path. More specifically, after the sealing sheet 150 is cut, the first liquid (F) may be located on a path where the first liquid (F) flows toward the second liquid (W) due to gravity.

The cutting unit 301 may function to cut the sealing sheet 150 by including a plurality of cutting parts 351 protruding upward. That is, the sample container 10 according to this embodiment may be configured so that the cutting portion 351 of the cutting unit 301 cuts the sealing sheet 150 from the bottom to the top. Accordingly, for example, the first liquid (F) may be poured into the second space (S2) by gravity, and the first liquid (F) and the second liquid (W) may be mixed. In an exemplary embodiment, when the first liquid (F) is formalin and the second liquid (W) is physiological saline, the concentration of formaldehyde in the mixture of the first liquid (F) and the second liquid (W) is about 6.0. % (v/v) to 15.0% (v/v), or about 7.0% (v/v) to 14.0% (v/v), or about 8.0% (v/v) to 13.0% (v/v) , or about 8.0% (v/v) to 12.0% (v/v).

As described above, the volume of formalin sealed within the cap 101 can be reduced by preparing formalin with a relatively high concentration as the first liquid (F). The present invention is not limited thereto, but for example, when the second liquid (W) is prepared by being injected by an operator, the weight of the sample container 10 containing only the first liquid (F) can be significantly reduced. there is. Therefore, there is an advantage in that a larger amount of sample containers 10 can be transported and transported at one time. In particular, if it is classified as a dangerous good such as formalin, there may be restrictions on its handling, transportation amount, storage amount, etc., and minimizing the volume and weight of formalin can serve as an advantage.

In addition, during the process of manufacturing or preparing a preservation solution with appropriate ingredients and concentration suitable for cell fixation, examiners such as clinicians and pathologists may be continuously exposed to formalin. The sample container 10 according to this embodiment maintains the first liquid (F) having a high concentration of formaldehyde containing harmful components in a sealed state, and the second liquid (W) for mixing additional components or diluting the concentration. ) has the effect of significantly reducing the frequency and time that workers are exposed to formaldehyde by having workers handle only them.

Additionally, the cutting unit 301 may have a cutting function and a filter function at the same time. The cutting unit 301 may allow liquid substances to pass through, but may not allow specimens such as cell tissue having a size larger than a predetermined size to pass through. For example, cell tissue accommodated in the container body 201 may not be able to move toward the cap 101. On the other hand, the first liquid (F) and the second liquid (W) may pass through the cutting unit 301 and move in opposite directions.

Specifically, the cutting unit 301 includes a second bottom portion 311 having a plurality of openings 311h, a third side wall portion 331 protruding upward from the second bottom portion 311, and a second bottom portion 311. ) and may further include a cutout portion 351 protruding upward from the second bottom portion 311, and a locking protrusion 371 protruding from the lower surface of the second bottom portion 311. The second bottom portion 311, the third side wall portion 331, the cutout portion 351, and/or the stopping protrusion 371 may be formed integrally with each other without physical boundaries.

The second bottom part 311, the third side wall part 331, the cut part 351, and the locking protrusion 371 at the bottom of the cutting unit 301 may be made of synthetic resin or the like. For example, the cutting unit 301 may be made of a material with lower rigidity than the cap 101 and/or the container body 201. For a specific example, the cutting unit 301 may be made of a material that is more elastic than the cap 101 and/or the container body 201.

The second bottom portion 311 may be approximately circular in plan view. The second bottom portion 311 may have a plurality of openings 311h. The openings 311h may have a regular matrix arrangement, a circular arrangement, or an irregular arrangement along the first direction (X) and the second direction (Y). In another embodiment, the opening 311h may be implemented in the form of radially arranged slits. In some embodiments, the collection of the plurality of openings 311h may be approximately circular. The second bottom portion 311 with the opening 311h may function as a filter plate. The size of the opening 311h can be appropriately selected considering the size of the specimen. For example, the length of the opening 311h in the first direction (X) (or the second direction (Y)) is about 10 mm or less, or about 5 mm or less, or about 4 mm or less, or about 3 mm or less, or about 2 mm. It may be less than or equal to about 1 mm, or less than or equal to about 0.5 mm.

The third side wall portion 331 may protrude upward from the second bottom portion 311. The third side wall portion 331 may have a substantially cylindrical shape with an open top. The outer diameter of the third side wall portion 331 is smaller than the inner diameter of the fastening body portion 121, and the inner diameter of the third side wall portion 331 is slightly smaller than the outer diameter of the first side wall portion 131, or is substantially the same. Or, it can be slightly larger. That is, the inner surface of the third side wall portion 331 may engage with the flat outer surface of the first side wall portion 131 to form an interference fit that maintains the coupled state through frictional force.

In some embodiments, the inner surface of the third side wall portion 331 may be plain without threads, etc. Through this, an interference fit (or fit) can be formed between the first side wall portion 131 of the cap 101 and the third side wall portion 331 of the cutting unit 301. In addition, through the combination of the cap 101 and the cutting unit 301, when the user rotates the cap 101, if a separate external force is not applied, the cutting unit 301 also remains coupled to the cap 101. and may be integrated so that they can rotate at least partially together.

Meanwhile, the coupling between the cap 101 and the cutting unit 301 may be rotationally unrestricted. That is, for example, in a state in which only the cap 101 and the cutting unit 301 are coupled without other components, the cutting unit 301 is based on the cap 101 (or the cap 101 is based on the cutting unit 301). E) Even if the cap 101 and the cutting unit 301 are rotated by more than about 90 degrees, more than about 180 degrees, or more than about 270 degrees in the plane, the cap 101 and the cutting unit 301 may not be separated from each other. In addition, when the cap 101 and the cutting unit 301 are coupled, the angle of rotation of the cap 101 and the cutting unit 301 on the plane can be unlimited, at an angle of 360 degrees or more.

On the other hand, the cap 101 and the cutting unit 301 may form a combination that allows insertion in the third direction (Z). For example, the cap 101 and the cutting unit 301 may be configured to be inserted in the third direction (Z) without rotation. That is, the degree of insertion of the cap 101 and the cutting unit 301 can be adjusted. In other words, even when the cap 101 is slightly inserted into the cutting unit 301, the cap 101 and the cutting unit 301 form a coupling relationship, and the cap 101 is inserted relatively into the cutting unit 301. Even when a lot of the cap 101 and the cutting unit 301 are inserted, a coupling relationship can be formed.

Through this, the insertion depth of the cap 101 into the cutting unit 301 can be adjusted simply by applying an external force in the third direction (Z), and further, the spacing between the cutting part 351 and the sealing sheet 150, which will be described later, can be adjusted. Distance can be adjusted. In some embodiments, the protrusion length of the third side wall portion 331 in the third direction (Z) is smaller than the protrusion length of the first side wall portion 131 in the third direction (Z), and the first side wall portion ( When 131 is completely inserted into the cutting unit 301, the lower end of the first side wall portion 131 may contact the upper surface of the second bottom portion 311.

The incision 351 may include a first incision 351a and a second incision 351b. From a plan view, the first cutout 351a may be arranged to at least partially surround the second cutout 351b. The protrusion height of the first cut portion 351a and the second cut portion 351b in the third direction (Z) may be smaller than the protrusion height of the third side wall portion 331 in the third direction (Z). That is, the height formed by the cutout portion 351 may be smaller than the height formed by the third side wall portion 331.

Specifically, the first cut portion 351a may form one or more cutting edges (or tips). 4 and the like illustrate a case where four first cut portions 351a are provided, but the present invention is not limited thereto, and in other embodiments, only one first cut portion 351a may be provided. The first cut portion 351a may cut the sealing sheet 150 using the tip. Unlike what is shown in the drawing, one first cut portion 351a may have a plurality of tips in the form of teeth or sawtooths rather than a single tip.

Additionally, each of the plurality of first cut portions 351a having a predetermined height may function like a partition. As will be described later, the plurality of first cut portions 351a are arranged in a substantially circular shape, but may be spaced apart from each other. A liquid substance, for example, the first liquid (F), may move to the outside or inside of the circularly arranged first cut parts 351a through the space between the first cut parts 351a.

The plurality of first cut portions 351a each have an outer surface and an inner surface, and the outer surface and the inner surface may each form a curved surface. In addition, the plurality of first cut parts 351a may have substantially the same shape, and the plurality of first cut parts 351a may be arranged in a circle with respect to the virtual center. That is, the plurality of first cut portions 351a may be arranged along the edge of the virtual circle. The virtual center may substantially coincide with the center of the circular second bottom portion 311. As a non-limiting example, one of the first incisions 351a may have a partial shape of a circular arc when viewed from a plan view.

Unlike what is illustrated in the drawing, in other embodiments, the number of first cut parts 351a may be two, three, or five, but even in this case, the circular arrangement angle between the plurality of first cut parts 351a It may be preferable that is substantially the same. In another embodiment, the plurality of first cut portions 351a are arranged in a circular manner based on the virtual center, but at least some of the first cut portions 351a may have different distances from the virtual center. .

The first cutting part 351a of the cutting unit 301 of the sample container 10 according to this embodiment has a curved outer surface and an inner surface, and is arranged in a circle with a virtual center to cut the sealing sheet 150. may be easier. As will be described later, when the sealing sheet 150 is cut by rotating the cap 101, the center of the circular arrangement of the first cut portion 351a and the center of rotation may coincide, and accordingly, the sealing sheet ( 150) can lead to uniform incision. Accordingly, from a plan view, the first liquid F can be poured approximately uniformly, thereby minimizing the increase in internal pressure of the sample container 10, and allowing the user to cut the sealing sheet 150 with minimal force. can do.

In some embodiments, from a plan view, a plurality of first cutouts 351a may be arranged to surround the opening 311h. That is, the first incisions 351a arranged in a circle can be divided into an outer area and an inner area, and the opening 311h can be located only in the inner area. At the moment the sealing sheet 150 is cut, the first liquid F in the first space S1 may move toward the second space S2 through the opening 311h.

In this case, since the volume inside the sample container 10, that is, the volume of the first space (S1) and the second space (S2), is not large, the first space (S1) and the second space (S2) are in instantaneous fluid communication. As this happens, the internal pressure inside the sample container 10 (that is, the area formed by fluid communication between the first space S1 and the second space S2) may increase. This may be because the first liquid F is poured to the lower side (second space S2 side), while the air in the lower side (second space S2 side) moves upward. If the increase in internal pressure is severe, liquid containing formaldehyde may leak in the form of bubbles through the joint portion of the cap 101 and the container body 201. This problem may not be a problem because complete sealing is possible if the bond between the cap 101 and the container body 201 is an irreversible bond, but if the bond between the cap 101 and the container body 201 is a reversible bond. It could be a problem. Therefore, it is necessary to suppress the increase in internal pressure of the sample container 10 as much as possible.

In this case, the inventors of the present invention confirmed that the increase in internal pressure of the container is influenced by the uniform penetration of the first liquid (F) and completed the present invention. As described above, the first cut portion 351a may be configured to cut the sealing sheet 150 from the bottom to the top to allow the first liquid F to pour out. That is, the cut or opening formed in the sealing sheet 150 is a position corresponding to the first cut part 351a on the plan, or is a position inside the cut by the second cut part 351b, which will be described later. Accordingly, from a plan view, the first liquid F is mainly poured through the inner area of the virtual circle where the first cut portion 351a is arranged.

Therefore, the increase in internal pressure of the container can be minimized by forming the opening 311h only in the inner area of the first cut part 351a, that is, in the area where liquid is expected to mainly pour. In other words, based on the first incision 351a, which functions as a partition, an opening is not formed on the outside through which only a relatively small amount of liquid material is poured, but an opening 311h is formed only on the inside through which the main liquid material is poured, The air movement path and the liquid movement path can be concentrated toward the center, and the increase in internal pressure of the container due to unexpected air flow can be minimized.

In addition, the second liquid W located in the first second space S2 may also contribute to suppressing the increase in internal pressure of the container. The second space S2 may have a sufficient volume to accommodate the mixture of the first liquid F and the second liquid W. In this case, as described above, when the first liquid F moves from the upper side to the lower side due to gravity, the air may move from the lower side to the upper side. At this time, if the volume of the second space S2 before the sealing sheet 150 is cut, specifically, the volume of air in the second space S2 is large, the degree of increase in the internal pressure of the container may increase. Therefore, this problem can be minimized by having the second liquid W occupy a predetermined volume in the initial state.

Meanwhile, the second cutout 351b may have an approximately '+' shape when viewed from a plan view. The second cutout 351b may also form one or more tips. For example, the second cutout 351b includes a first part extending in one direction and a second part extending in a direction perpendicular thereto, and the intersection of the first part and the second part is the tip (tip). ) can be formed. The second cutting part 351b can cut the sealing sheet 150 using the tip.

Additionally, the '+' shaped second cut part 351b may contact the circularly arranged first cut part 351a. Accordingly, the circular area where the opening 311h is located (the inner area of the first cutout 351a) can be divided into approximately four areas. By forming a '+' shaped partition using the second cut portion 351b, the amount of liquid material permeating between each of the four areas can be approximately uniform and the increase in internal pressure can be minimized. In some embodiments, the second cutout 351b may not overlap the opening 311h in the third direction (Z). That is, an opening may not be formed at the location where the second cutout 351b is formed.

The cutting unit 301 of the sample container 10 according to this embodiment includes not only a circularly arranged first cutting part 351a but also a second cutting part 351b having a flat side to further seal the sealing sheet 150. It can be easily cut. As will be described later, the sealing sheet 150 may be cut by relative rotation between the cutting unit 301 and the cap 101. In this process, the first cut part 351a is arranged in a circle about the center coinciding with the axis of rotation to facilitate easy cutting, and the second cut part 351b, which has a flat side, uses its tip to form a sealing sheet 150. ) and then use the side to push and rotate the cut sealing sheet 150. Accordingly, there is an effect of further reducing the force required to cut the sealing sheet 150.

In some embodiments, the maximum height of the second cut part 351b is smaller than the maximum height of the first cut part 351a, and at the moment the sealing sheet 150 and the cut part 351 approach, the second cut part 351b ) may be configured to first contact the sealing sheet 150 with the first cut portion 351a other than the first cut portion 351a. It is required that the first cut part 351a, which cuts the sealing sheet 150 using the tip, penetrates the sealing sheet 150 first, rather than the second cut part 351b, which cuts the sealing sheet 150 by pushing it open. This can be advantageous in terms of reducing the force.

Meanwhile, a plurality of locking protrusions 371 may be disposed on the lower surface of the second bottom portion 311. The locking protrusion 371 may protrude from the lower surface of the second bottom portion 311. Figure 5 illustrates a case in which four locking protrusions 371 are arranged in a circular arrangement at approximately 90 degrees. As described above, only one locking protrusion 371 may be provided, but one of the support portion 251 and the locking protrusion 371 may be provided in plural numbers. The number of locking protrusions 371 will be described in detail later.

The locking protrusion 371 may be configured to interfere with the support portion 251. That is, when the cutting unit 301 is placed on the support part 251, the upper surface of the support part 251 is in contact with the lower surface of the second bottom part 311, and the upper surface of the support part 251 is in contact with the locking protrusion 371. It can be located on the upper side of the bottom. In other words, the locking protrusion 371 and the support portion 251 together can prevent the cutting unit 301 from rotating beyond a predetermined angle with respect to the container body 201.

In an exemplary embodiment, when four locking protrusions 371 are disposed, rotation of the cutting unit 301 by more than 90 degrees with respect to the container body 201 may be restricted. That is, the cap 101 and the cutting unit 301 are already coupled, and in the process of screwing the cap 101 and the container body 201, the cap 101 is separated from the container body 201 as described above. Even if rotated by about 360 degrees, the cutting unit 301 can be rotated in a range of about 270 degrees or less based on the container body 201. In other words, when the cap 101 is rotated, the cutting unit 301 may be configured to rotate with the cap 101 for an initial predetermined angle, but not to rotate for a predetermined angle thereafter. The lower limit of the first predetermined angle may be about 20 degrees or more, about 30 degrees or more, or about 40 degrees or more. Additionally, the upper limit of the first predetermined angle may be less than about 270 degrees, or less than about 180 degrees, and preferably less than about 90 degrees.

In other words, when the cap 101 is rotated 360 degrees based on the container body 201, the cap 101 and the cutting unit 301 are coupled for an initial predetermined angle (e.g., about 89 degrees). The cutting unit 301 may be rotated in conjunction with the cap 101 while maintaining its state. Accordingly, the cutting unit 301 can also rotate about 89 degrees.

On the other hand, for a predetermined angle (e.g., about 271 degrees) thereafter, only the cap 101 rotates 271 degrees in its relative position with the container body 201, and the cutting unit 301 has the locking protrusion 371 and the support portion ( 251), further rotation may not occur due to interference.

Expressed another way, when the cap 101 is rotated 360 degrees based on the cap 101, the cutting unit 301 is interlocked with the cap 101 for the first predetermined angle (about 89 degrees). Thus, the position of the cutting unit 301 may not change in the relative position between the cap 101 and the cutting unit 301.

On the other hand, during the subsequent predetermined angle (about 271 degrees), the cutting unit 301 may rotate about 271 degrees with respect to the cap 101 in its relative position with the cap 101.

The sample container 10 according to this embodiment may be configured to rotate the cap 101 sufficiently for complete sealing, but the cutting unit 301 may rotate less than that. In an exemplary embodiment, the difference between the maximum rotation angle of the cap 101 relative to the container body 201 and the allowable rotation angle of the cutting unit 301 may be less than 360 degrees.

The maximum rotation angle of the cap 101 can be adjusted through the extension length of the first screw thread 121s and the second screw thread 221s. As described above, the maximum rotation angle of the cap 101 refers to the rotation angle when the first screw thread 121s and the second screw thread 221s are completely screwed.

Additionally, the allowable rotation angle range of the cutting unit 301 may be adjusted through the arrangement angle and/or number of the locking protrusion 371 and the support portion 251. Specifically, when both the locking protrusions 371 and the supports 251 are arranged circularly at a uniform angle, the allowable rotation angle range of the cutting unit 301 is one of the number of locking protrusions 371 and the number of supports 251. It may be less than the numerical value of 360 degrees for large numbers. If there are four locking jaws and four supports, four locking jaws and one support part, or one locking jaw and four support parts, the allowable rotation angle range of the cutting unit 301 may be less than 90 degrees. . In another embodiment, when there are three locking protrusions and one support part, the allowable rotation angle range of the cutting unit 301 may be less than 120 degrees. In another embodiment, when there are two locking protrusions and one support part, the allowable rotation angle range of the cutting unit 301 may be less than 180 degrees.

Accordingly, the sample container 10 according to this embodiment can reduce the force required to cut the sealing sheet 150. As described above, when the user holds the container body 201 and rotates the cap 101, the cutting unit 301 also rotates the cap 101 for an initial predetermined angle (permissible rotation angle range of the cutting unit 301). ) (i.e., the cutting unit 301 does not rotate with respect to the cap 101), but when the rotation angle is exceeded, the cutting unit 301 rotates with respect to the cap 101. do. Therefore, compared to the case where the cutting unit 301 rotates based on the cap 101 from the beginning, rotational inertia can be formed in the cap 101 as in the present invention, enabling easier cutting of the sealing sheet 150. It becomes.

In addition, it is possible to prevent the sealing sheet 150 from being completely cut beyond being cut by the cut portion 351. That is, the difference between the maximum rotation angle of the cap 101 with respect to the container body 201 and the allowable rotation angle of the cutting unit 301 is formed to be less than 360 degrees, so that the sealing sheet 150 is completely separated into two or more parts. Instead, it may be configured to be only partially incised so that the first liquid (F) can flow out. If the sealing sheet 150 is partially cut, problems such as the cut portion free from the bottom of the first side wall 131 blocking the opening 311h may occur, which may lead to an increase in the internal pressure of the container. On the other hand, the present invention, although not limited thereto, has the effect of preventing the sealing sheet 150 from being completely cut through the rotation angle control along with the number, shape, and arrangement of the cut portions 351.

Hereinafter, the structure, connection relationship, arrangement relationship, and function of the sample container 10 according to this embodiment will be described in more detail with further reference to FIGS. 6 to 11. Figures 6 to 11 are diagrams showing the process of using the sample container 10 of Figure 1.

Specifically, FIG. 6 is a cross-sectional view showing the initial state of the sample container 10, and FIG. 7 is a cross-sectional view showing the step of injecting the sample (SP) into the sample container 10. In addition, FIG. 8 is a cross-sectional view showing the sample SP in a sealed state, and FIG. 9 is a rear perspective view showing the arrangement relationship between the container body 201 and the cutting unit 301 in FIG. 8. In addition, Figure 10 is a cross-sectional view showing the sealing sheet 150 in a cut state, and Figure 11 is a rear perspective view showing the arrangement relationship between the container body 201 and the cutting unit 301 in Figure 10.

First, referring further to FIG. 6, the blocking ring 401 is placed on the ring support portion 221k of the container body 201, the cutting unit 301 is coupled to the bottom of the cap 101, and the cap 101 The cap 101 and the container body 201 are coupled using the first screw thread 121s of the container body 201 and the second screw thread 221s of the container body 201.

As described above, the blocking ring 401 is inserted and coupled between the fastening body portion 121 and the ring support portion 221k of the cap 101, and the first side wall portion 131 and the cutting unit 301 of the cap 101. ) of the third side wall portion 331 is engaged with each other and press-fitted, and the cap 101 and the container body 201 may be coupled by screwing. Additionally, the first screw thread 121s and the second screw thread 221s may be only partially coupled by the blocking ring 401.

In this embodiment, the lower surface of the second bottom portion 311 of the cutting unit 301 may be in contact with the upper surface of the support portion 251 of the container body 201. In addition, the first liquid F (i.e., preservation liquid) containing formaldehyde is accommodated in the first space S1, and the second space S2 of the container body 201 is sealed by the cap 101. may be in a state in which a second liquid W containing sodium chloride (eg, physiological saline) is accommodated. In this step, the cut portion 351 of the cut unit 301 (only the second cut portion 351b is shown in the cross-sectional view) may be spaced apart from the sealing sheet 150 in the third direction (Z).

In another embodiment, the cutting unit 301 may not be coupled to the bottom of the cap 101 but may be placed on the support portion 251. In this case, as will be described later, the cap 101 and the cutting unit 301 may be fitted during the process of rotating the cap 101 and moving it downward. In another embodiment, the cutting unit 301 may be coupled to the lower end of the cap 101 and may be spaced apart from the support portion 251 in the third direction (Z). In this case, as will be described later, in the process of rotating the cap 101 and moving the cap 101 and the cutting unit 301 downward, the cutting unit 301 may come into contact with the support portion 251.

Next, further referring to FIG. 7, the screw fastening of the cap 101 and the container body 201 is loosened, and the sample (SP) is introduced into the open second space (S2) of the container body 201. The specimen (SP) may be human cells, tissue, sputum, oral wash, etc., but the present invention is not limited thereto. As described above, since the second liquid W is already accommodated in the second space S2, it may be easy to inject the sample SP using biopsy forceps or the like.

At this stage, the cutting unit 301 may still be coupled to the cap 101, and the blocking ring 401 may be placed on the ring support 221k.

Next, further referring to FIGS. 8 and 9, the blocking ring 401 is removed and the cap 101 and the container body 201 are partially coupled. In this step, the coupling may be a screw tightening coupling caused by rotation of the first screw thread (121s) and the second screw thread (221s). In addition, the sample (SP) is accommodated and stored in the second space (S2) of the container body (201) sealed by the cap (101) and can be completely isolated from the outside.

Specifically, in the process of partially combining the first screw thread 121s and the second screw thread 221s, that is, in the process of rotating the cap 101 with respect to the container body 201, the cutting unit 301 is the cap ( 101) can be integrated and moved together. In other words, the separation distance between the sealing sheet 150 and the cutting unit 351 does not substantially change, the cap 101 and the cutting unit 301 are moved downward together, and the distance between the cap 101 and the cutting unit 301 is moved downward. There may be no relative rotation. In addition, as shown in FIG. 9, the locking protrusion 371 of the cutting unit 301 and the support portion 251 of the container body 201 are initially spaced apart, and then the cap 101 and the cutting unit 301 are rotated. Accordingly, the locking protrusion 371 and the support portion 251 may gradually become closer.

Next, further referring to FIGS. 10 and 11, the cap 101 and the container body 201 are completely coupled. In this step, the coupling may be a screw tightening coupling caused by rotation of the first screw thread (121s) and the second screw thread (221s).

Specifically, in the process of completely combining the first screw thread 121s and the second screw thread 221s, that is, in the process of further rotating the cap 101 with respect to the container body 201, the cutting unit 301 and the cap ( The relative position and rotation state of 101) may vary. In other words, while the cap 101 gradually moves downward by tightening the screw, the lower surface of the cutting unit 301 may be prevented from moving downward as it comes into contact with the support portion 251. Accordingly, the distance between the sealing sheet 150 and the cut portion 351 gradually becomes closer, and the cut portion 351 may penetrate the sealing sheet 150 and partially cut it. In addition, as shown in FIG. 11, the cutting unit 301 does not rotate with respect to the container body 201 due to interference between the locking protrusion 371 of the cutting unit 301 and the support portion 251 of the container body 201. It may not be possible. In other words, the cap 101 and the cutting unit 301 rotate relative to each other, thereby reducing the force required to cut the sealing sheet 150.

As the sealing sheet 150 is cut, the first space (S1) and the second space (S2) are in fluid communication, and the first liquid (F) contained in the first space (S1) of the cutting unit (301) It may pour into the second space (S2) through the opening (311h). The first liquid (F) and the second liquid (W) are mixed with each other to form a mixed liquid (FW), and the sample (SP) can be fixed or preserved by the mixed liquid (FW). The concentration of formaldehyde in the mixed solution (FW) is about 6.0% (v/v) to 15.0% (v/v), or about 7.0% (v/v) to 14.0% (v/v), or about 8.0% ( v/v) to 13.0% (v/v), or about 8.0% (v/v) to 12.0% (v/v).

The sample container 10 according to this embodiment accommodates different liquids in the first space (S1) and the second space (S2), which are separated from each other, to reduce the frequency and time of exposure of workers to harmful substances such as formaldehyde. It can be minimized. In addition, by configuring the rotational relationship between the cap 101, the cutting unit 301, and the container body 201 to be within a specific range, the force required to cut the sealing sheet 150 can be minimized, and the internal pressure inside the container increases. It has the effect of suppressing.

Although the above description focuses on the embodiments of the present invention, this is only an example and does not limit the present invention, and those skilled in the art will understand the present invention without departing from the essential characteristics of the embodiments of the present invention. It will be apparent that various modifications and applications not exemplified above are possible.

Therefore, the scope of the present invention should be understood to include changes, equivalents, or substitutes of the technical ideas exemplified above. For example, each component specifically shown in the embodiments of the present invention can be modified and implemented. And these variations and differences related to application should be construed as being included in the scope of the present invention as defined in the appended claims.

101: cap
111: ceiling part
121: fastening body part
121s: first thread
131: first side wall portion
150: sealing sheet
201: container body
211: first bottom portion
221: second side wall portion
221k: ring support
221s: second thread
251: support part
301: cutting unit
311: second bottom portion
311h: opening
331: Third side wall portion
351: incision
401: blocking ring
S1: first space
S2: Second space
F: first liquid
W: second liquid

Claims (2)

a main body including a main body bottom portion and a main body side wall portion extending upward from the main body bottom portion; a cap configured to form a screw-fastening connection with the main body; and a filter having a filtering aperture,
When the cap and the filter are combined, during the process of screwing the cap and the main body,
A sample container configured to prevent rotation of the filter with respect to the main body due to interference between the filter and the main body. According to paragraph 1,
During the process of further screwing the cap and body after the rotation of the filter is stopped,
(a) The cap further rotates relative to the main body,
(b) the vertical separation distance between the bottom of the body and the cap is further reduced,
(c) A sample container in which the vertical separation distance between the bottom of the main body and the filter is substantially maintained.

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