KR20240055195A - Panaxana ginsan, ginseng extract obtained by extracting and purifying ginseng comprising ginseng saponin having effect of immune enhancement, fatigue recovery and blood curculation improvement, and ginseng polyssaccharides having effect of anti-cancer, immune enhancement, hematopoieses improvement, and radiation protection as standard compound together and method for manufacturing the same - Google Patents

Abstract

The present invention uses as indicator ingredients the active ingredients of Korean ginseng, especially ginseng saponin, which is effective in improving immunity, fatigue recovery, and improving blood circulation, and ginseng polysaccharide, which has anticancer, immune enhancement, hematopoiesis promotion, and radiation protection effects. It relates to panaxena ginic acid, a ginseng extract containing high content, and its manufacturing method. Ginseng extract, which is one aspect of the present invention, contains a high content of active ingredients and can exhibit excellent effects when applied to the human body. In addition, the method for producing ginseng extract, which is an aspect of the present invention, is efficient because it can extract the active ingredient from ginseng with high yield in a simple method.

Description

Panaxena, a ginseng extract obtained by extracting and purifying ginseng saponin derived from Korean ginseng, which has the effect of enhancing immunity, relieving fatigue, and improving blood circulation, and ginseng polysaccharide, which has anti-cancer, immune-boosting, hematopoiesis-promoting, and radiation-protective effects, as an indicator ingredient. Jinsan and its manufacturing method ES IMPROVEMENT, AND RADIATION PROTECTION AS STANDARD COMPOUND TOGETHER AND METHOD FOR MANUFACTURING THE SAME}

The present invention uses as indicator ingredients the active ingredients of Korean ginseng, especially ginseng saponin, which is effective in improving immunity, fatigue recovery, and improving blood circulation, and ginseng polysaccharide, which has anticancer, immune enhancement, hematopoiesis promotion, and radiation protection effects. It relates to a ginseng extract containing high content and a method for producing the same.

Korean ginseng (Panax ginseng C.A. Meyer) is a specialty medicinal plant belonging to the Papaceae family and is a perennial herb that grows about 50 to 80 cm in height. Korean ginseng has been widely used as a nutritional tonic for a long time, and with the recent development of analytical science, research on the types and functions of ingredients contained in Korean ginseng is actively underway. Representative physiologically active substances of Korean ginseng include ginsenoside, a ginseng saponin of the triteropenoid family, polyacetylene with anticancer activity, phenolic compounds with antioxidant activity, and radiation protection. It contains ginseng proteins, acidic polysaccharides with immunomodulating properties, and other nutrients such as vitamins, sugars, and minerals, and is known to have excellent rejuvenation, immunity enhancement, nutritional tonic, and anti-cancer effects. .

Meanwhile, since ancient times, major consumers of Korean ginseng have been highly interested in the function (efficacy) of Korean ginseng, and thus products containing Korean ginseng currently on the market are promoting excellent efficacy as a marketing point. However, among the products containing Korean ginseng currently on the market, there is no product that can fully convey the excellent efficacy of ginseng to ginseng users. Cases of damage to consumers due to excessive advertising are accumulating, and consumers are turning their attention to health functional foods other than Korean ginseng. As it continues to circulate, it is having a negative impact on the entire Korean ginseng-related industry. Accordingly, the present inventors provided a method for effectively extracting useful ingredients from Korean ginseng and a high-performance Korean ginseng extract obtained thereby, thereby serving as a driving force for new vitality and revival in the domestic Korean ginseng industry, which has been devastated and declining for a long time. By doing so, we aim to instill pride in Korean ginseng farmers and workers in the Korean ginseng industry, and seek to create wealth through this, and once again establish our status as the home country of Korean ginseng in the hearts of people around the world who are suffering from the COVID-19 pandemic. I want to stand up straight.

KR 10-2022-0083404 A

In one aspect, the purpose of the present invention is to provide a ginseng extract with excellent efficacy.

In one aspect, the purpose of the present invention is to provide a ginseng extract containing a high content of active ingredients, especially ginseng saponins and polysaccharides.

In one aspect, the purpose of the present invention is to provide a ginseng extract containing a high content of polysaccharides of excellent quality.

In one aspect, the purpose of the present invention is to provide a ginseng extract with excellent human stability and safety.

In one aspect, the purpose of the present invention is to provide a ginseng extract that can be used in the production of food compositions for humans as well as food compositions for animals.

In order to achieve the above object, the present invention in one aspect is a ginseng extract, wherein the ginseng extract contains 65% by weight or more of polysaccharides, based on the total weight of the extract; and 10% by weight or more of saponin, wherein the polysaccharide of the ginseng extract contains polysaccharides with a molecular weight of 3,500 to 2,000,000 in an amount of 55% or more based on the total weight of the polysaccharide.

In addition, the present invention provides a ginseng extract comprising one or more of the following in the above aspect:

i) Polysaccharides with a molecular weight of 35,000 to 45,000 are included at 15 to 20% by weight relative to the total weight of the polysaccharide, ii) Polysaccharides with a molecular weight of 160,000 to 200,000 are included at 17 to 23% by weight relative to the total weight of the polysaccharide, iii) Molecular weights are 1,500,000 to 2,000,000 phosphorus polysaccharide is included in an amount of 0.5 to 1.5% by weight based on the total weight of the polysaccharide, iv) a polysaccharide with a molecular weight of 35,000 to 45,000 contains more than 70% by weight of glucose or less than 3% by weight of protein compared to the molecular weight of the polysaccharide, v ) Polysaccharides with a molecular weight of 160,000 to 200,000 contain more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide; and vi) the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contains more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide.

In addition, in order to achieve the above object, the present invention provides a ginseng extract in which the total content of ginsenosides Rg1 and Gb1 contained in the ginseng extract is 2.5% by weight or more based on the total weight of the extract.

Additionally, in order to achieve the above object, the present invention provides a food composition containing the ginseng extract.

Additionally, in one aspect, the present invention provides a method for producing the ginseng extract, including the step of extracting ginseng, in order to achieve the above object.

In the above aspect, the present invention provides that the step of extracting ginseng includes: ginseng whole plant extraction step; And it provides a method for producing a ginseng extract, including a ginseng foil extraction step.

In addition, in order to achieve the above object, the present invention includes that the ginseng whole plant extraction step and the ginseng foil extraction step are performed by solvent extraction, and the solvent used in the ginseng whole plant extraction step includes water, and the ginseng foil extraction step includes: The solvent used in the extraction step includes an organic solvent and an aqueous solution thereof, providing a method for producing a ginseng extract.

In addition, the present invention provides a method for producing a ginseng extract, wherein the ginseng whole plant extraction step is performed under one or more of the following conditions: i) extraction temperature: 80 to 95°C, ii) extraction time: 6 to 12 hours, iii) Extraction solvent and volume: water, 3 to 8 times the weight of ginseng whole plant.

In addition, the present invention provides a method for producing a ginseng extract, wherein the ginseng peel extraction step is performed under one or more of the following conditions: i) extraction temperature: 50°C or less, ii) concentration of extraction solvent: 60-90% (v/v) aqueous alcohol solution, and iii) extraction time: 3 to 6 hours.

In addition, the present invention, in the above aspect, the method for producing the ginseng extract further comprises the step of mixing the extract obtained from the ginseng whole plant extraction step and the extract obtained from the ginseng foil extraction step, the ginseng extract Provides a manufacturing method.

The ginseng extract, which is one aspect of the present invention, contains a high content of active ingredients such as ginseng polysaccharide and ginseng saponin, and can exhibit excellent effects when applied to the human body. In addition, the method for producing ginseng extract, which is an aspect of the present invention, is efficient because it can extract the active ingredient from ginseng with high yield in a simple method.

Figure 1 is a diagram showing the measurement results of ginsenoside standard substances of ginseng extract according to the present invention.
Figure 2 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 1,726,284.
Figure 3 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 181,813.
Figure 4 shows the results of HPLC analysis of ginseng polysaccharide with a molecular weight of 40,592.

Hereinafter, the present invention will be described in detail.

The following description is only an example, and the scope of the present invention is not limited by the following.

In addition, the terms or words used in the specification and claims of the present invention are not to be construed as limited to their ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his/her invention in the best way. It must be interpreted with meaning and concept consistent with the technical idea of the present invention based on the principle that it can be done.

Throughout the specification of the present invention, when a part is said to "include" a certain component, this means that it does not exclude other components but may further include other components unless specifically stated to the contrary. .

The present invention relates to ginseng saponin ingredients (ginsenosides), which are notified health functional ingredients recognized as being effective in improving immunity, relieving fatigue, and improving blood circulation among the ingredients contained in Korean ginseng, and non-ginseng saponin-based substances, Ginseng polysaccharide component, which has recently been recognized as an important substance in the field of alternative medicine and has been proven to have excellent efficacy in the field of biological immune response modulators, which is rapidly growing globally, and Korean ginseng extract that controls both components simultaneously as an indicator component. It is named Panaxana Ginsan. Through the present invention, the present inventors have developed a manufacturing technology capable of mass producing Panaxena Jinic Acid with high purity, and have established a range of evaluation criteria that defines the characteristics and range of Panaxena Jinic Acid manufactured through this method, and have developed Panaxena Jinic Acid into an immune system. It is intended to be used as a main material for medicines and foods that can have direct and indirect curative effects on various immune-related diseases, including cancer diseases, through enhancement, fatigue recovery, blood circulation improvement, anti-cancer immunity enhancement, hematopoiesis promotion, radiation protection effect, etc. .

Korean ginseng is a perennial herb belonging to the genus Ginseng of the Papaceae family, and its roots are mainly used in oriental medicine. Among them, Koryo ginseng grown in Korea is well known to have much better medicinal properties than ginseng grown in China, Japan, and the United States. In the case of saponin, which is the main physiologically active component of ginseng, since Shibata et al. isolated 13 types using methanol extracts of ginseng, more than 30 types have been isolated and identified, and have anti-pain, anti-cancer, anti-diabetic, liver function-enhancing effects, anti-thrombosis, and anti-inflammatory properties. It is known to be a major ingredient with various physiological activities such as inflammation. Other non-saponin components also exhibit various physiological activities, so the effects mentioned in oriental medicine are too diverse to list. Recently, many studies have been conducted on various physiological activities such as antioxidant activity, platelet aggregation inhibition ability, cancer cell toxicity, and heavy metal removal ability. there is.

In the case of ginseng saponins, they are called ‘ginsenosides.’ This is a compound word combining 'ginseng', meaning ginseng, and 'glycoside', meaning ginseng glycoside. Unlike common herbal saponins, ginseng saponins have a dammaran skeleton of triterpenoids, so while saponins derived from other plants are polar and have toxicity such as hemolysis, since they are neutral glycosides, they are not toxic and have pharmacological effects. Unlike the saponins of this common herbal medicine, it has a wide variety of physiological activities. Ginseng saponin components are largely divided into the panaxadiol family, which has a sedative effect, and the panaxatriol family, which has a stimulating effect. Korean ginseng contains more types of saponins than foreign ginsengs such as American ginseng, Panax ginseng, and Jukjeol ginseng, and the panaxadiol family. It is known to have an excellent role in regulating the balance of the body, such as blood pressure and body temperature, due to its balanced ratio of panaxatriol and panaxatriol. Saponin is a component widely found in over 750 species of plants, and is named so because it produces fine bubbles like soap in an aqueous solution. Ginseng saponin is a compound in which sugars such as glucose, xylose, arabinose, and rhamnose are ether bonded to certain regions of the non-saccharide portions PPD and PPT, and its molecular weight is approximately between 800 and 1,200. Ginsenosides are divided into dozens of types depending on the sugar bonding structure. Depending on the difference in migration distance in thin layer chromatography (TLC), ginsenoside Ro, ginsenoside Ra, ginsenoside Rb1, ginsenoside Rb2, ginsenoside They are named ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rg2, ginsenoside Rg3 and ginsenoside Rh1.

Known effects of ginseng saponins include enhancing the anti-decomposition effect of insulin, anti-lipolysis action, and improving symptoms associated with diabetes. Triol-based saponins in ginseng have a somewhat positive effect on the occurrence of type 1 diabetes, and diol-based saponins have an anti-inflammatory effect. It delays the onset of type 1 diabetes and significantly reduces the incidence. It not only increases the insulin content in the pancreas and blood, but also suppresses the infiltration of lymphocytes into the pancreas. Ginseng saponin ginsenoside Rb2 suppresses urinary albumin excretion, suppresses kidney weight and enlargement of glomeruli. Regarding the anti-cancer effect, ginseng saponin increases the body's resistance by increasing the immune function of cancer patients, thus suppressing the growth of cancer cells and metastasis to other organs, and showing excellent efficacy in preventing recurrence after surgery. It is known, and according to the research results of Murata et al., ginseng concentrate containing a large amount of saponin was administered to cancer patients such as stomach cancer, pancreas cancer, colon cancer, and breast cancer for a long period of 3 to 6 months, and as a result of clinical observation, 69.7% of the patients showed cancer. It was confirmed that the progression and development of was suppressed. In addition, Gusan et al. administered ginseng saponin to various cancer patients and confirmed the effect of improving subjective symptoms, recovering blood, and suppressing cancer progression. According to the results of investigating the relationship between the frequency of taking ginseng and the probability of developing cancer, long-term use of ginseng was found to be effective in suppressing cancer progression. The research results showing that the probability of developing cancer significantly decreases the more you use it, it can be seen that there is a long-term non-specific cancer prevention effect. In addition, ginseng saponin can be said to be a mysterious elixir because it normalizes blood pressure by raising it in case of low blood pressure and lowers blood pressure in high blood pressure, thus maintaining the blood pressure at a normal level. Among ginseng saponins, it is a component that has the effect of raising blood pressure. (Rg group) and components with blood pressure lowering effects (Rb, Rc group) coexist, which acts to normalize blood pressure overall. Taking Korean ginseng in patients with high blood pressure reduces systolic and diastolic blood pressure, lowers low-density cholesterol in the blood in patients with hyperlipidemia, improves lipid metabolism by raising high-density cholesterol, which plays a good role in cholesterol metabolism, and improves endothelial damage caused by high blood pressure. Improves cellular dysfunction. Since the blood pressure lowering effect of ginseng is mainly due to ginseng saponin, the effect is greater when taken in relatively large quantities rather than in small amounts by selecting ginseng concentrate with a relatively high saponin content. Ginseng saponin has the effect of lowering blood pressure, causing parasympathetic It normalizes the balance of the autonomic nervous system by relieving nerve tension as needed, promoting blood flow and smoothing the supply of blood to the brain, making it effective in treating low blood pressure. In addition, ginseng saponins have a vasodilating effect, improving blood circulation, inhibiting the development of arteriosclerosis and protecting against damage to vascular endothelial cells. Ginseng saponins have enzyme activities related to lipid metabolism such as fat and blood cholesterol. It has the effect of promoting and preventing the formation of atheroma caused by hyperlipidemia in the aortic tissue, and has the effect of improving hyperlipidemia, an important risk factor for arteriosclerosis, by promoting cholesterol turnover. Ginseng saponin components (especially Rb2) are effective in reducing blood cholesterol content and promoting excretion, preventing vascular lesions caused by hypercholesterolemia, and improving arteriosclerosis indicators by reducing LDL-cholesterol and increasing HDL-cholesterol. It has been proven.

Meanwhile, ginseng extract contains various sugars. Polysaccharides, which are monosaccharides including glucose and fructose, disaccharides, trisaccharides, tetrasaccharides, pentasaccharides including maltose and saccharides, and many monosaccharides combined with each other, are also found in ginseng (Ovodov et al. 1966). In early research until the 1960s, little chemical research was conducted on ginseng polysaccharides, which are polymeric substances, and little research was conducted on the physiological functions of ginseng saccharides. However, with the recent development of life sciences, carbohydrates have been used as simple biological structural materials and As it becomes known that it plays an important role not only as an energy source but also as a signal transmitter for biological functions, its importance is being re-recognized and research on it is intensifying. In particular, polysaccharides and their derivatives from plants and microorganisms are being developed as immunomodulatory substances, and oligosaccharides are also being actively developed as medical agents such as anti-inflammatory agents and antibacterial agents and functional food materials.

Research on the polysaccharide components of ginseng and their effectiveness was pursued much later than saponin research due to the structural complexity of these components. In 1984, Hikino and Tomoda of Japan were the first to isolate and report the polysaccharide components panaxan A, B, C, D, and E from Korean Korean ginseng (Tomoda et al. 1984, 1985, Konno et al. 1984). Afterwards, as researchers continued to study, a total of 21 species of panaxan, from panaxan F to panaxan U, were isolated (Konno et al. 1985, 1987, Hikino et al. 1985, Oshima et al. 1985). Meanwhile, these polysaccharide components are combined with the same or different monosaccharides, and the main constituent sugars differ depending on the type of polysaccharide, but include glucose, arabinose, galactose, xylose, and rhamnose, etc. Among them, panaxan A is composed of glucose (92.1%) and a small amount of peptide (1.7%) and has a molecular weight of approximately 14,000, while panaxan B contains 95.9% glucose and 0.7% peptide and is a peptidoglycan with a molecular weight of approximately 1,800,000. The structure is being revealed (Tomoda et al. 1984, 1985). It has been revealed through animal experiments that the physiological activity of these polysaccharide components has a hypoglycemic effect that significantly suppresses hyperglycemia induced by treatment with alloxan, an experimental diabetes-inducing substance (Konno et al. 1984). , Ng et al. 1985). Yang et al. also reported that when polysaccharide components isolated from ginseng were administered to experimental animals (rats) (50-200 mg/kg, intraperitoneal, subcutaneous injection), they lowered blood sugar levels, reduced liver glycogen content, and promoted insulin secretion ( Yang et al. 1990). Additionally, there are reports that polysaccharide components isolated from ginseng improve immune function. It is known that the anti-tumor activity of most polysaccharide components is not directly toxic to cancer cells, but is due to an immune-modulating effect. Currently, in China, considering that the polysaccharide fraction of ginseng is used clinically as an anticancer agent, attempts are being made to isolate polysaccharide components from ginseng roots and leaves and study their pharmacological activity. Wang et al. administered ginseng polysaccharide components orally or intraperitoneally to normal and cancer-causing animals (mice) and found that it had the effect of enhancing the foreign substance phagocytosis ability of the reticuloendothelial system, which is responsible for immune function, and promoting antibody production. It was also reported that these ingredients had an improvement effect in clinical application to cancer patients (Wang et al. 1985). The water-soluble and alkaline (0.5 M NaOH)-soluble polysaccharide fractions isolated from the roots and leaves of ginseng have anti-complementary activity, and among these fractions, the acidic polysaccharide fraction showed the strongest activity ( Gao et al. 1989). The complement system plays an important role in immunity, inflammation, and allergic reactions, and polysaccharides with anti-tumor effects are reported to have anti-complement activity (Okuda et al. 1972). The content of crude acidic polysaccharides is higher in roots than in leaves. Their chemical properties are different; the roots mainly contain pectin and glucan, while the leaves contain pectin and heteroglycan (Gao et al. 1989). However, the chemical structure of these acidic polysaccharides was not revealed, and through further advanced research, two new acidic polysaccharide components, namely ginsenan PA and ginsenan PB, were recently isolated from water-soluble extracts of Korean ginseng, which have remarkable anticomplement activity. It has the effect of enhancing the activity of the intrareticulum system, which is responsible for immune function, by phagocytosing foreign substances coming from the outside (Tomoda et al. 1993). The molecular weights of these components are approximately 16,000 and 5,500, respectively, and their chemical structural forms are α-arabino- It has structural units of -3,6-galactan type and rhamno galacturonan type. Ginsenan PA is composed of L-arabinose, D-galactose, L-rhamnose, D-galacturonic acid (11:22:1:6:1, molar ratio) and 1.3% O-acetyl group, which are decomposition products of ginsenan PA. As a result of examining the phagocytic activity and anti-complement activity of In Korea, research on the efficacy of ginseng polysaccharide components in relation to cancer immunity is being pursued with interest. Regarding immunotoxicity of cyclosphamide (CY), an anticancer drug, ginseng polysaccharide fraction administered to mice was effective in suppressing immunotoxicity (reduction in number of hemolytic plaque forming cells, number of white blood cells, spleen weight, etc.) caused by CY treatment (Kim et al. al, 1990). In addition, administration of ginseng polysaccharide components suppressed the development of cancer in mice transplanted with cancer cells (Sarcom-180) and showed an increase in hemolytic plaque forming cells (PFC) related to antibody production and an enhancement of phagocytic activity in the reticular system. Strong activity was observed in the acidic polysaccharide fraction (Kim et al. 1990, 1991). Meanwhile, Okuda et al. in Japan isolated acidic polysaccharides from water extracts of Korean red ginseng through research on physiologically active substances other than saponin contained in Korean red ginseng over the past 10 years. The chemical structure of this ingredient was found to have an α-1,4-polygalturonan skeleton similar to pectin with several acetoxy groups (Lee et al. 1990, Okuda 1990). In general, cancer causes weight loss, and it is known that the weight loss of cancer patients is due to the decomposition of body fat and the effect of causing loss of appetite by toxohormone-L secreted by cancer cells. The acidic polysaccharide component of ginseng causes this toxohormone. (Okuda et al. 1984, Okuda 1989). In addition, these acidic polysaccharide components inhibit pancreatic lipase (lipolytic enzyme) activity and cholesterol esterase activity and reduce serum neutral lipids. It has been shown to be active. Since this action can inhibit or delay the absorption of sugars or fats from the intestines, it is expected to be effective in preventing so-called obesity or hyperlipidemia (Okuda et al. 1993). Briefly describing the results of research on ginseng polysaccharide at the Korea Atomic Energy Research Institute, ginseng polysaccharide increases the ability of macrophages to produce IL-1 and IL-12 in the biological immune mechanism, and the action of this factor increases IL-2 production in Th cells. As the ability to produce cytokines such as and IFN-γ increases, the responsiveness of various cells to these cytokines increases, and the activity of cytotoxic T cells, LAK cells, NK cells, macrophages, etc. increases. Additionally, by proliferating bone marrow cells, it reduces side effects caused by various anticancer drugs or radiation that cause bone marrow failure. Looking at the anti-cancer immune enhancement effect more specifically, the production of nitric oxide (NO) in the culture medium was increased as a result of culturing mouse intraperitoneal macrophages in FCS-RPMI1640 medium containing ginseng acid, and the production of nitric oxide (NO) in the culture medium was increased when mouse intraperitoneal macrophages were cultured in ginseng acid-added FCS-RPMI1640 medium. When cultured with tumor cells Yac-1 for 48 hours, the growth of Yac-1 cells was inhibited. When 100 mg/kg of ginseng polysaccharide was intravenously administered to Balb/c mice, NK activity increased 2.4 times compared to the non-administered group, and the proliferation ability of T lymphocytes increased when 100 mg/kg of ginseng polysaccharide was intravenously administered to Balb/c mice. Responsiveness of cells to mitogen (Concanavalin A 2.5㎍/ml) increased 2.1-fold. In the case of LAK cell production ability, mouse spleen cells were cultured in vitro in FCS-RPMI1640 medium supplemented with 0.5㎍/㎖ of ginseng polysaccharide. The killing activity against tumor cell YAC-1 was measured and was 10 times stronger than that of the control group. Ginseng polysaccharide not only exhibits anti-cancer immune activity by promoting the secretion of various types of cytokines, but also functions to maintain the normal state when the autonomous balance of various cytokines is abnormal, and Th1 type cytokines (IL-2, (IFNγ) and Th2 type (IL-4, IL-5, IL-10) cytokines are maintained in balance. In diseases such as asthma and allergies, Th2 secretion increases, and in rheumatoid arthritis, Th1 secretion increases. It is caused by the assembly and reaction of cells, and the regulation of these cytokines can be said to be a very important factor. When mouse spleen cells were cultured in FCS-RPMI1640 medium supplemented with 50㎍/㎖ of ginseng polysaccharide, IL-1, IL-12, The mRNA expression of IL-2, IFN, CSF, and TNFα increased, and when 100 mg/kg of ginseng polysaccharide was administered intravenously to Balb/c mice, the production capacity of cytokines such as GM-CSF, IL-12, IFNγ, and TNFα in the serum was increased. This has increased significantly. When B16-F10 melanoma, a type of lung cancer cell, was injected intraperitoneally 3 days later, 10 mg/kg of ginseng polysaccharide was injected intraperitoneally eight times every other day, metastasis of cancer cells to the lung was inhibited by 44%. When the LLC lung cancer cell line was subcutaneously injected into mice and the size of 1cm3 was reached, 100 mg/kg of ginseng polysaccharide was administered intravenously to inhibit the growth of 59.3% of cancer cells. The 30-day survival rate of mice transplanted with the LLC lung cancer cell line was 12.5% in the control group. It increased to 80% in the ginseng polysaccharide administered group. In addition, looking at the number of bone marrow cells on the 5th day of mice injected intravenously with 100 mg/kg of ginseng polysaccharide and then irradiated with a sublethal dose, there was significant recovery of myeloblasts and recovery of differentiated granular-mononuclear cells. Confirmed. Hematopoietic progenitor cells undergo a process of maturation and differentiation into granulocytes, lymphocytes, erythrocytes, platelets, etc. under the influence of various hematopoietic factors and the bone marrow microenvironment. In general, inflammation and infection due to bone marrow failure occur during radiation treatment in cancer patients. This is a common cause of side effects, but when 100 mg/kg of ginseng polysaccharide was injected intravenously, the production capacity of white blood cells, neutrophils, lymphocytes, and platelets in the peripheral blood was increased, and the radiation exposure of mice administered 100 mg/kg of ginseng polysaccharide 24 hours before radiation exposure was increased. The lethal dose (LD50/30) was 7.46 Gy in the control group to 10.76 Gy in the ginseng polysaccharide administered group, increasing the survival rate of bone marrow cells by 1.44 times. Therefore, ginseng polysaccharide can increase the effectiveness of ginseng when used in combination with radiation, both in terms of increasing the radiation therapy dose for cancer treatment and reducing side effects. In addition, the 20-day survival rate of mice administered 100 mg/kg of ginseng polysaccharide intravenously 48 hours before cyclophosphamide (intraperitoneal injection) increased from 20% in the control group to 60% in the ginseng polysaccharide-administered group, indicating the significance of granulocytes in peripheral blood due to ginseng polysaccharide administration. It is thought that this increase improves the side effects of anticancer drugs such as cyclophosphamide.

In one aspect, the present invention relates to a ginseng extract, wherein the ginseng extract contains 65% by weight or more of polysaccharides, based on the total weight of the extract; and 10% by weight or more of saponin, and the polysaccharide of the ginseng extract is a ginseng extract containing polysaccharides with a molecular weight of 3,500 to 2,000,000 in an amount of 55% or more based on the total weight of the polysaccharide.

In one aspect, it is generally known that about 95% of the polysaccharide contained in ginseng is composed of glucose.

In the above aspect, the content of glucose contained in the ginseng extract of the present invention may be 60% or more, based on the total weight of the extract. In one embodiment, the total content of glucose contained in the ginseng extract of the present invention may be 69.3%.

In addition, in one aspect, the content of polysaccharides contained in the ginseng extract of the present invention may be 65 to 75% by weight, or 67 to 75% by weight, or 67 to 73% by weight, or 67 to 71% by weight, or 68% by weight. It may be ~70% by weight.

In addition, in one aspect, the content of saponin contained in the ginseng extract of the present invention is 10 to 20% by weight, or 13 to 20% by weight, or 13 to 18% by weight, or 15 to 18% by weight, based on the total weight of the extract. It may be %.

In addition, in one aspect, the polysaccharide having a molecular weight of 3,500 to 2,000,000 among the polysaccharides included in the ginseng extract of the present invention is 55 to 75% by weight, or 55 to 65% by weight, or 57 to 65% by weight, based on the total weight of the polysaccharide. %, or 59 to 65% by weight, or 59 to 63% by weight, but is not limited thereto.

Additionally, in one aspect, the ginseng extract may include polysaccharides with a molecular weight of 35,000 to 45,000 in an amount of 15 to 20% by weight based on the total weight of the polysaccharides. Specifically, polysaccharides having a molecular weight of 35,000 to 45,000 may be included at 16 to 19% by weight, or 16 to 18% by weight, based on the total weight of the polysaccharide.

In addition, in one aspect, the ginseng extract may contain polysaccharides with a molecular weight of 160,000 to 200,000 in an amount of 17 to 23% by weight relative to the total weight of the polysaccharides. Specifically, polysaccharides with a molecular weight of 160,000 to 200,000 may include 17 to 23% by weight relative to the total weight of the polysaccharides. It may be included in weight%, or 18 to 20 weight%.

In addition, in one aspect, the ginseng extract may contain polysaccharides with a molecular weight of 1,500,000 to 2,000,000 in an amount of 0.7 to 1.2% by weight relative to the total weight of the polysaccharides, specifically, 0.7 to 1.1% by weight, or 0.7 to 1.0% by weight, or It can be included at 0.8 to 1.0% by weight.

In addition, in one aspect, the ginseng extract may include one or more of the following characteristics: i) the polysaccharide having a molecular weight of 35,000 to 45,000 contains 70% by weight or more of glucose or 3% by weight or less of glucose relative to the molecular weight of the polysaccharide. Contains protein, ii) Polysaccharides with a molecular weight of 160,000 to 200,000 contain more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide, iii) Polysaccharides with a molecular weight of 1,500,000 to 2,000,000 contain the corresponding Contains more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide.

Specifically, the polysaccharide having a molecular weight of 35,000 to 45,000 may contain more than 70% by weight of glucose based on the molecular weight of the polysaccharide. Specifically, the polysaccharide having a molecular weight of 35,000 to 45,000 may contain more than 73% by weight of glucose, or more than 75% by weight of glucose based on the molecular weight of the polysaccharide. For example, the content of glucose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 75 to 83% by weight, or 77 to 81% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 35,000 to 45,000 contained in the ginseng extract may further include galactose, araminose, and rhamnose. The content of galactose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 2 to 8% by weight, or 2 to 6% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 5 to 15% by weight, or 7 to 15% by weight, or 9 to 13% by weight, based on the total weight of the polysaccharide. Or it may be 10 to 12% by weight. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 35,000 to 45,000 may be 1 to 6% by weight, or 2 to 6% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 160,000 to 200,000 contained in the ginseng extract may contain 80% by weight or more of glucose relative to the molecular weight of the polysaccharide. Specifically, the content of glucose contained is, It may be 85 to 95% by weight, or 87 to 95% by weight, or 87 to 93% by weight, or 89 to 93% by weight, relative to the molecular weight. In addition, the polysaccharide with a molecular weight of 160,000 to 200,000 contained in the ginseng extract may further include galactose, araminose, and rhamnose. The content of galactose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 0.5 to 5% by weight, or 0.5 to 4% by weight, or 1 to 3% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 0.1 to 2% by weight, or 0.1 to 1.5% by weight, or 0.1 to 1% by weight, based on the total weight of the polysaccharide. Or it may be 0.3 to 0.7 weight%. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 160,000 to 200,000 may be 1 to 10% by weight, or 3 to 9% by weight, or 3 to 5% by weight, based on the total weight of the polysaccharide.

In addition, in the above aspect, the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract may contain more than 80% by weight of glucose relative to the molecular weight of the polysaccharide. Specifically, the content of glucose contained is, It may be 85 to 95% by weight, or 87 to 95% by weight, or 87 to 93% by weight, or 88 to 92% by weight, relative to the molecular weight. In addition, the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract may further include galactose, araminose, rhamnose, and xylose. The content of galactose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 5 to 15% by weight, or 7 to 13% by weight, or 7 to 11% by weight, based on the total weight of the polysaccharide. In addition, the content of araminose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.01 to 0.1% by weight, or 0.01 to 0.1% by weight, or 0.01 to 0.07% by weight, based on the total weight of the polysaccharide. Or it may be 0.01 to 0.05% by weight. In addition, the content of rhamnose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.001 to 0.1% by weight, or 0.005 to 0.015% by weight, or 0.007 to 0.013% by weight, based on the total weight of the polysaccharide. In addition, the content of xylose contained in the polysaccharide having a molecular weight of 1,500,000 to 2,000,000 may be 0.1 to 0.5% by weight, or 0.1 to 0.3% by weight, or 0.1 to 0.2% by weight, based on the total weight of the polysaccharide.

In addition, each of the polysaccharides with a molecular weight of 35,000 to 45,000, the polysaccharide with a molecular weight of 160,000 to 200,000, and the polysaccharide with a molecular weight of 1,500,000 to 2,000,000 contained in the ginseng extract of the present invention may contain proteins. The protein content contained in each of these may be 3% by weight or less based on the total weight of the polysaccharide, and in particular, the protein content contained in the polysaccharide with a molecular weight of 35,000 to 45,000 is 2% by weight based on the total weight of the polysaccharide. It may be below.

Additionally, in one aspect, the ginseng extract of the present invention may contain Rg 1 and Gb 1 in an amount of 2.5% by weight or more based on the total weight of the extract. Specifically, the total amount of Rg 1 and Gb 1 contained in the ginseng extract may be 2.5 to 3% by weight, or 2.5 to 2.8% by weight, or 2.5 to 2.7% by weight, based on the total weight of the extract.

In one embodiment, the polysaccharide having a molecular weight of 1,726 or 284 may be included in an amount of 0.5 to 1% by weight, or 0.7 to 1% by weight, or 0.85 to 0.95% by weight, based on the total weight of the polysaccharide.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 562,356 may be included in an amount of 3.5 to 5% by weight, or 3.5 to 4.7% by weight, or 4 to 4.7% by weight, or 4 to 4.5% by weight, based on the total weight of the polysaccharide. there is.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 181,813 may be included in an amount of 17 to 23 wt%, or 18 to 22 wt%, or 19 to 20 wt%, based on the total weight of the polysaccharide.

Additionally, in one embodiment, the polysaccharide having a molecular weight of 40,592 may be included in an amount of 13 to 21 wt%, or 14 to 20 wt%, or 15 to 19 wt%, or 16 to 18 wt%, based on the total weight of the polysaccharide. there is.

In addition, in one embodiment, the polysaccharide having a molecular weight of 6,038 may be included in an amount of 5 to 11% by weight, or 6 to 11% by weight, or 7 to 10% by weight, or 7 to 9% by weight, based on the total weight of the polysaccharide. there is.

In addition, in one embodiment, the polysaccharide having a molecular weight of 3,555 is 8 to 15% by weight, 9 to 15% by weight, 9 to 13% by weight, or 10 to 13% by weight, or 11 to 13% by weight, based on the total weight of the polysaccharide. It may be included in weight percent.

In one aspect, the ginseng extract of the present invention can exhibit excellent efficacy by containing the above substances as described above.

In one aspect, the present invention is a food composition containing the ginseng extract.

The formulation of the food composition is not particularly limited, but may be formulated into, for example, tablets, granules, pills, powders, liquids such as drinks, caramels, gels, bars, tea bags, etc. In addition to the active ingredients, the food composition of each formulation can be appropriately selected and mixed by a person skilled in the art according to the formulation or purpose of use with ingredients commonly used in the field, and can have a synergistic effect when applied simultaneously with other raw materials. can happen. Additionally, the food may be a health functional food.

The composition can be administered in various ways, such as simple ingestion, drinking, injection, spray administration, or squeeze administration.

In the food composition according to one aspect of the present invention, the determination of the dosage of the active ingredient is within the level of a person skilled in the art, and may vary depending on various factors such as the age of the subject to be administered, health status, complications, etc. .

In addition to the above, the food composition according to one aspect of the present invention contains various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavors, colorants and enhancers (cheese, chocolate, etc.), pectic acid, and the like. It may include salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. In addition, food compositions according to one aspect of the present invention may include pulp for the production of natural fruit juice, fruit juice beverages, and vegetable beverages. These ingredients can be used independently or in combination. The ratio of these additives is not that important, but is generally included in the range of 0 to about 60 parts by weight per 100 parts by weight of the composition according to one aspect of the present invention.

Additionally, in one aspect, the food composition may be for humans, or may be for animals, specifically for livestock, or for pets.

Additionally, in one aspect, the present invention may be a pharmaceutical composition containing the ginseng extract.

The pharmaceutical composition may further contain pharmaceutical auxiliaries such as preservatives, stabilizers, wetting agents or emulsification accelerators, salts and/or buffers for osmotic pressure adjustment, and other therapeutically useful substances, and may be administered orally according to a variety of conventional methods. It can be formulated as a dosage form or parenteral dosage form.

The oral administration agents include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, etc., and these dosage forms contain surfactants in addition to the active ingredients. , may contain diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycol). . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, and in some cases starch, agar, alginic acid or its sodium salt. It may contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavoring agents, and sweeteners. The tablets can be prepared by conventional mixing, granulating or coating methods. In addition, the parenteral administration may be in the form of rectal, topical, subcutaneous, or transdermal administration, for example, injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, and suppositories. It may be in the form of a patch, etc., but is not limited thereto. The pharmaceutical composition according to one embodiment of the present specification may be administered topically, for example, to the scalp.

Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug varies depending on various factors such as the degree of symptom progression of the subject to be administered, time of onset, age, health condition, and complications, but is based on adults. In general, 1 μg/kg to 200 mg/kg of the composition, preferably 50 μg/kg to 50 mg/kg, can be administered in divided doses 1 to 3 times a day, and the dosage can be administered by any method. It does not limit the scope of this specification.

Additionally, the present invention may be a cosmetic composition containing the ginseng extract.

The external form of the cosmetic composition may contain a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, such as solutions, gels, solids, pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and It may be provided in the form of a non-ionic vesicular dispersant, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions can be prepared according to conventional methods in the art. The compositions according to the present disclosure may also be prepared in the form of a foam or in the form of an aerosol further containing a compressed propellant.

The cosmetic composition is not particularly limited in its formulation, and includes, for example, softening lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing foam, cleansing water, It can be formulated into cosmetics such as packs, powders, body lotions, body creams, body oils, or body essences.

When the formulation of the cosmetic composition is paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. It can be.

When the formulation of the cosmetic composition is powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon, May contain propellants such as propane/butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a solvent, solvating agent, or emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene. These include fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tracant may be used.

When the formulation of the cosmetic composition is a cleansing agent containing a surfactant, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid. Amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linoline derivative, or ethoxylated glycerol fatty acid ester can be used.

In one aspect, the pharmaceutical composition and/or food composition may be used for purposes such as improving immunity, enhancing physical vitality, restoring energy, improving bone and joint health, anticancer, promoting hematopoiesis, protecting the body from radiation, anti-inflammatory, etc. .

In one aspect, the present invention is a method of producing the ginseng extract. The method may include extracting ginseng.

In one aspect, the step of extracting ginseng includes a ginseng whole plant extraction step; And it may include a ginseng foil extraction step.

As used herein, “ginseng powder” may refer to the residue remaining after first extracting the active ingredient from ginseng whole plant.

In other words, the ginseng foil extraction step may refer to the step of extracting the remaining ginseng residue after performing the ginseng outgrowth extraction step.

In one aspect, the ginseng whole plant extraction step and the ginseng foil extraction step may include being performed by solvent extraction.

The solvent used in the ginseng whole plant extraction step may include water, and the solvent used in the ginseng peel extraction step may include one or more of an organic solvent and an aqueous solution thereof.

In one aspect, the ginseng whole plant extraction step may be performed including one or more of the following conditions: i) extraction temperature: 80 to 95°C, ii) extraction time: 6 to 12 hours, iii) amount of extraction solvent ( volume): 3 to 8 times the weight of ginseng whole plant.

For example, the ginseng whole plant extraction step can be performed for 6 to 12 hours using water at a temperature of about 85 to 95 ° C as a solvent, and at this time, the extraction solvent is added about 3 to 8 times based on the weight of the ginseng whole plant. It can be done.

In one aspect, the ginseng whole plant extraction step may further include washing the ginseng, and crushing the ginseng. The crushing step may include being performed so that the particle size of the ginseng shredded material is about 2 to 5 mm.

Additionally, in one aspect, after the ginseng whole plant extract step is performed, the step of separating the ginseng whole plant extract and the ginseng powder may be further included. At this time, the step of separating the ginseng foil may include cooling the temperature of the ginseng extract to about 50° C. or lower and then separating the ginseng foil and the ginseng extract using centrifugal dehydration or a filter press.

In addition, the ginseng peel extraction step may be performed under one or more of the following conditions: i) extraction temperature: 50°C or less, ii) concentration of extraction solvent: 60 to 90% (v/v) aqueous alcohol solution and iii) Extraction time: 3 to 6 hours.

In one aspect, the organic solvent used in the ginseng peel extraction step is not particularly limited, and is a low C ₁ to C ₅ solvent such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, n-butanol, and isobutanol. Alcohol, polyhydric alcohols such as glycerol, ethylene glycol, propylene glycol, and 1,3-butylene glycol, hydrocarbon solvents such as methyl acetate, ethyl acetate, benzene, n-hexane, diethyl ether, dichloromethane, and chloroform, and petroleum It may be a non-polar organic solvent such as ether, methyl acetate, benzene, hexane, chloroform, methylene chloride, dimethyl ether, and ethyl acetate.

In one embodiment, the solvent used in the ginseng peel extraction step of the present invention may be an aqueous ethanol solution.

The concentration of the ethanol aqueous solution may be 50 to 90% (v/v), 60 to 90% (v/v), or 70 to 80% (v/v).

Additionally, in one aspect, the ginseng foil extraction step of the present invention may be performed using a low-temperature extraction, for example, using a solvent of about 50° C. or lower, and may be performed for about 3 to 6 hours.

In one aspect, the method may further include removing the extraction solvent contained in the extract obtained in the ginseng foil extraction step. For example, the solvent (ethanol) can be removed by distillation at 50-70°C in a reduced pressure environment.

Additionally, in one aspect, the method for producing the ginseng extract may further include mixing the extract obtained from the ginseng whole plant extraction step and the extract obtained from the ginseng foil extraction step.

Additionally, in one aspect, the method for producing a ginseng extract of the present invention may further include the step of removing substances other than the target substance. Specifically, heavy metal substances that may be present in the extract can be removed by passing the extract through a filtration device (e.g., activated carbon filtration device), and the temperature of the extract can be adjusted to remove proteins, carbohydrates, and fibers floating in the extract. After cooling to 5°C or lower, centrifugation can be performed at a speed of 15,000rpm or higher.

In addition, a filtration process can be further performed to remove microorganisms (bacteria, etc.) and inactive polysaccharides with a molecular weight of 2,000,000 or more in the ginseng extract. The filtration process can be repeated several times by passing through a system equipped with a TFF (tangential flow filtration) membrane with a size of 0.2 micrometers.

Additionally, in one aspect, the method may include concentrating the extract. The concentration step can be performed in a reduced pressure concentration device, and the concentration is performed at a temperature of about 75 to 85 ° C until the sugar content reaches about 8 to 10 Brix.

Meanwhile, during the concentration process, proteins dissolved in the extract may be leached, which can be removed through centrifugation, etc. as described above.

In one aspect, the ginseng extract prepared by the above method may be formulated in powder form or liquid form. For example, the ginseng extract can be frozen at a temperature of about -60°C, then heated and maintained several times in a vacuum for 48 hours, and then powdered while raising the temperature to 40°C. In the case of liquid, it can be formulated as a concentrate to have a sugar content of 10 to 65 Brix using a reduced pressure concentration device at a concentration temperature that does not exceed 95°C.

In one aspect, when following the production method of the present invention as described above, a large amount of active ingredients can be extracted from ginseng in an easy way, making it economical.

Hereinafter, the present invention will be described in detail through preparation examples and examples. These examples are merely for illustrating the present invention, and the scope of the present invention is not limited by these examples.

[Preparation example] Preparation of ginseng extract

① Wash the phosphorus ginseng in water to remove any dirt or foreign substances, put it in a crusher, crush it into coarse particles with a particle size of about 2 to 5 mm, place it in an extraction bag, and compare it to the phosphorus ginseng weight. Hot water extraction was performed by adding 3 to 8 times the weight of water, setting the extraction temperature to 85 to 95°C, and setting the extraction time to 6 to 12 hours from the time the extraction temperature was reached.

② After extraction was completed, the temperature of the ginseng hot water extract was cooled to 50°C or lower, and then the extraction bag containing the ginseng foil was pressed to separate the ginseng hot water extract and the ginseng foil.

③ In order to extract as much of the ginseng saponin remaining in the ginseng peel as possible, the ginseng peel separated in step 2 is placed back in the extraction bag, then placed in a low-temperature reflux extraction device, and the solvent is mixed at a volume ratio of ethanol: water of 3:1. After that, the extraction bag containing the ginseng gourd was placed in a low-temperature reflux extraction device to the extent that it was submerged. Low-temperature reflux extraction was performed for about 3 to 6 hours by controlling the extraction temperature of the solvent below 50°C at room temperature and continuously circulating the solvent in a shower-type spray form within the low-temperature reflux extraction device to maximize extraction efficiency.

④ After step 3 is completed, the extraction bag containing the ginseng gourd is removed, the ginseng reflux extract is put into a vacuum distiller, and the ethanol is distilled at a distillation temperature of 50-70°C under reduced pressure, cooled, and recovered to produce the ginseng reflux extract. The alcohol ethanol used as a solvent was removed.

⑤ The refluxed ginseng extract from which the ethanol was removed was mixed with the ginseng hot water extract from step 4 to complete the extraction of the ginseng extract desired in the present invention.

⑥ In order to remove heavy metal substances present in the ginseng extract, the heavy metal substances were adsorbed and removed through an activated carbon filtration device. In addition, in order to remove particulates such as proteins, carbohydrates, and fiber floating in the ginseng extract, the ginseng extract is cooled to below 5℃, and when the rotation speed of the ultra-high-speed centrifuge reaches over 15,000 rpm, the cooled ginseng extract is ultra-high-speed centrifuged. The particulates to be removed by putting them into the separator were accumulated in the rotating body, and the ginseng extract was separated and purified while being discharged through the discharge port at the top of the rotating body.

⑦ In order to remove bacterial microorganisms and inactive polysaccharide substances with a molecular weight of 2,000,000 or more present in the ginseng extract, the product is permeated after passing through an Ultra-filtering system equipped with a TFF (Tangential Flow Filtration) 0.2㎛ membrane (Millipore). The ginseng extract is collected as the target ginseng extract and passed through the ultra-filtering system. The ginseng extract that is not filtered and retentated is diluted with water several times and filtered through the ultra-filtering system repeatedly several times to recover as much of the target ginseng extract as possible and then discarded. did.

[Experimental example]

[Experimental Example 1] Glucose content analysis

Since about 95% of polysaccharide components, including acidic polysaccharides derived from ginseng, are composed of glucose, the glucose content was analyzed to determine the polysaccharide content of ginseng extract (Panaxana Ginsan).

1. Weigh exactly 2g of ginseng extract powder sample, place it in an Erlenmeyer flask, and add 50ml of distilled water to completely dissolve.

2. Add 4 ml of concentrated Hcl (36%) to the completely dissolved sample.

3. For acid decomposition, place an Erlenmeyer flask in a reflux condenser with a sealed top and heat in a water bath at 80°C for 3 hours.

4. When acid decomposition is completed, leave to cool with the mouth of the Erlenmeyer flask blocked, then add 4 ml of 36% NaOH and add a small amount of 1N-NaOH to neutralize it to around pH 7.

5. Put the sample in a 100 ㎖ mass flask and fill it with distilled water up to the 100 ㎖ mark. (Extraction solvent dilution factor in the calculation formula: 100 ㎖)

6. Insert a folded filter paper into a triangular funnel and filter the sample to remove insoluble substances.

7. Dilute the filtered sample according to the dilution factor (x2, x4, x8, x16, x32, x64, x128) specified in the test tube.

8. Add 1 ml of each diluted sample to another test tube and add 1 ml of D.N.S (Dinitro salicylic acid) reagent.

9. Heat the test tubes containing the D.N.S reagent in boiling water for 5 minutes, then leave to cool. Add 8 ml of distilled water to each test tube and mix well with a Voltex mixer.

10. Set the measurement wavelength of the spectrophotometer to 540 nm, measure the measurement by inserting a quartz cell containing distilled water, adjust the set reference value (O.D = 0), then place the sample in the quartz cell and measure the absorbance.

11. The O.D value according to the dilution factor of the sample is shown in Table 1 below.

dilution factor X 2 X 4 X 8 X 16 X 32 X 64 X 128 O.D value 2.342 1.617 0.905 0.653 0.338 0.184 0.089

12. In order to convert the measured O.D value into a sample concentration value, the above operation is repeated using a glucose standard product to measure the absorbance value for each glucose concentration to obtain a glucose standard sample concentration value. Confirm the calculation formula.

13. The O.D value according to the dilution factor of the sample is calculated as the sample concentration value based on glucose, as shown in the table below.

(Calculation formula) Sample concentration value = (O.D value + 0.0141)/0.0007

Dilution factor X 2 X 4 X 8 X 16 X 32 X 64 X 128 Sample concentration value 3365.9 2330.1 1313.0 953.0 503.0 283.0 147.3

14. Using the sample concentration value, dilution factor, and extraction solvent dilution factor, calculate the glucose content of the analysis sample using the formula below.

<Equation>

Dilution factor X 2 X 4 X 8 X 16 X 32 X 64 X 128 Glucose content (%) 33.7 46.6 52.5 76.2 80.5 90.6 94.3 Glucose content average value (5); Excluding dilution factor x2 and x128 69.3

As a result, it was confirmed that the ginseng extract of the present invention contains about 69.3% glucose.

[Experimental Example 2] Molecular weight distribution analysis of polysaccharide

1. Accurately weigh 50 mg of ginseng extract powder sample, place in a test tube, add 5 ml of distilled water, and completely dissolve using a Voltex mixer.

2. Put the dissolved sample in the syringe and pass it through a 0.45㎛ nylon syringe filter to complete the preparation of the sample for GPC analysis.

<Configuration of analysis equipment>

1. Pump: Shimadzu LC-20AD

2. Injector: Shimadzu SIL-20A

3. Column Oven: Shimadzu CTO-20A

4. Column: PSS Suprema 10000Å + 3000Å + 30Å

5.Detector: ELSD

<Analysis conditions>

1. Flow Rate: 1.0 ㎖/min

2. Injection Volume: 50㎕

3. Oven Temperature: 40℃

4. Analysis Time: 40 ~ 50min

<Finding Calibration Curve and Function>

1. Use PSS Dextrane 1,400,000 333,000 164,000 35,600 8,100 908 as a standard sample and prepare it in the same way as the analysis sample above.

2. Calibration Curve

[Figure 1]

3.Function

f(x)=7.655291e ^-004 *X^3-3.181748e ^-002 *X^2+0.1386024*X+6.373336(X=x ^-T.LIMIT ) R^2=0.9999212 Dispersion=0.0093897 T.LIMIT=15min

4. Calibration Table

# Time(min) Molecular Weight Error(%) Active One 21.452 1400000 -0.6085 Enabled 2 24.375 333000 3.4946 Enabled 3 25.431 164000 -2.5961 Enabled 4 27.732 35600 -2.0459 Enabled 5 29.925 8100 2.0257 Enabled 6 33.246 908 -0.4096 Enabled

The results are as shown in Table 5 below.

Peak# Ret. Time Weight Average Molecular Weight Area(%) One 19.98 2,243,426 1.68 2 20.35 1,726,284 0.87 3 24.87 562,356 4.25 4 25.52 181,813 19.43 5 27.64 40,592 17.08 6 30.15 6,308 8.54 7 32.07 3,555 12.36 8 34.96 536 35.34 9 37.88 107 0.45 Area(%) Total 100.00 Peak number (Mw 2,000,000 ~ Mw3,500) 6 Area(%) 62.53

[Experimental Example 3] Analysis of sugar composition of ginseng polysaccharide

1. From the above GPC analysis results, 30 ml of each ginseng polysaccharide component present at retention times of 20 minutes, 25 minutes, and 27 minutes was collected in a test tube and powdered in a freeze dryer.

2. 1 g of ginseng polysaccharide powder sample was dissolved in 10 ml of distilled water, 12 ml of 60% perchloric acid was added, and the sample was extracted and hydrolyzed in boiling water for 4 hours.

3. The hydrolyzed ginseng polysaccharide solution was neutralized with NaOH solution, 40 ml of ethanol was added to 10 ml of the decomposition solution, centrifuged at 5,000 r.p.m for 20 minutes, and the supernatant was collected.

4. The supernatant was concentrated at 60°C to remove ethanol, dissolved in distilled water, filtered through a Millipore filter (pore size 0.45㎛), and analyzed using HPLC (High Performance Liquid Chromatography).

5. D-glucose, L-arabinose, D-galactose, xylose, and L-ramrose from Merck were used as sugar standards, and the HPLC analysis conditions are as shown in the table below.

Instrument Waters HPLC/ALC-244 Flow rate 1.0㎖/min Packing material Lichrosorb NH ₂ (Merck) Chart speed 0.5㎝/min Column 4.6(i.d.) Detector RI, 8X Mobile phase Acetonitrile/H ₂ O (80/20)

The results are shown in Tables 7 to 9 below.

[HPLC analysis results of ginseng polysaccharide with molecular weight of 1,726,284]

Type per composition Retention Time (minutes) content(%) glucose 2.7 90.52 galactose 3.5 9.15 Arabinose 3.8 0.04 rhamnose 4.3 0.01 xylose 4.9 0.16

[HPLC analysis results of ginseng polysaccharide with molecular weight of 181,813]

Type per composition Retention Time (minutes) content(%) glucose 3.1 91.82 galactose 3.3 2.48 Arabinose 3.8 0.52 rhamnose 4.6 4.60 xylose - 0

Type per composition Retention Time (minutes) content(%) glucose 3.1 79.51 galactose 3.4 4.08 Arabinose 3.8 11.42 rhamnose 4.8 3.81 xylose - 0

6. In addition, in order to verify the results of HPLC analysis of the three components of the separated ginseng polysaccharides, the glucose content, a representative sugar, was measured using the above glucose content analysis method.

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 1,726,284]

Dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 5.565 2.915 1.505 0.767 0.395 0.196 0.094

* Number of grams of sample: 2g

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 7970.0 4185.0 2170.0 1116.3 584.4 300.6 154.1

* Calculation formula: Sample concentration value = (O.D value +0.0141)/0.0007

Dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 79.7 83.7 86.8 89.3 93.5 96.2 98.6 Excluding dilution factor x2 and x128 89.9

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 181,813]

Dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 5.719 2.982 1.529 0.780 0.403 0.199 0.095

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 8190.0 4280.0 2205.0 1133.8 596.3 304.1 155.5

Dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 81.9 85.6 88.2 90.7 95.4 97.3 99.5 Excluding dilution factor x2 and x128 91.4

[Glucose content analysis results of ginseng polysaccharide with a molecular weight of 40,592]

Dilution factor X2 X4 X8 X16 X32 X64 X128 O.D value 4.907 2.604 1.344 0.679 0.342 0.171 0.082

Dilution factor X2 X4 X8 X16 X32 X64 X128 Sample concentration value 7030.0 3740.0 1940.0 990.0 509.4 264.7 137.2

dilution factor X2 X4 X8 X16 X32 X64 X128 Glucose content (%) 70.3 74.8 77.6 79.2 81.5 84.7 87.8 Excluding dilution factor x2 and x128 79.6

As a result, the results of the glucose content analysis method and the HPLC analysis method showed similar results, ensuring the reliability of the sugar content analysis value by the HPLC analysis method.

[Experimental Example 4] Analysis and evaluation of ginseng saponin content of ginseng extract (Panaxana Ginsan)

1. Weigh exactly 1g of ginseng extract powder sample and place it in an Erlenmeyer flask, then add 60ml of distilled water and completely dissolve.

2. Put 60 ml sample into 250 ml separator reed, add 60 ml ether, shake several times to extract lipids, then separate layers.

3. When the separation of the ether layer and the sample layer in the separatory funnel is completed, the ether layer is removed, then 60 ml of saturated butanol is added and the saponin is extracted by shaking several times.

4. When the separation of the saturated butanol layer and the sample layer in the separatory funnel is completed, the upper saturated butanol layer is recovered and placed in a 250 ml Erlenmeyer flask, and the sample is placed back into the separatory funnel.

5. Repeat experimental operations 3 and 4 a total of 3 times and collect 180 ml of saturated butanol layer in a 250 ml Erlenmeyer flask.

6. Discard the sample for which saponin extraction has been completed, add 180 mL of saturated butanol layer back into the separation reed, and then add 50 mL of distilled water to wash the saturated butanol layer.

7. Place 180 ml of the washed saturated butanol layer into a weighed Erlenmeyer flask, attach the Erlenmeyer flask to an evaporator, immerse it in a water bath at 80°C, and concentrate under reduced pressure.

8. Place the Erlenmeyer flask with completed reduced pressure concentration in a dry oven at 105°C, dry it for about 15 minutes, cool it in the desiccator, and then measure the weight of the Erlenmeyer flask containing crude ponin again.

<Equation>

Erlenmeyer flask weight after concentration (g) 126.6877 Empty Erlenmeyer flask weight (g) 126.5230 Ginseng saponin (%) 16.47 Ginseng saponin (mg/g) 164.7

[Experimental Example 5] Analysis and evaluation of ginseng components (Ginsenosides) of ginseng extract (Panaxana Ginsan)

1. Ginsenoside standard substances (Rb1, Rg1, Re, Rd, Rc, Rb2) were weighed at 50 mg each in a 50 mL volumetric flask, and then diluted with 70% methanol to prepare a standard stock solution (1 mg/mL). Make a standard solution by appropriately diluting the stock solution with 70% methanol.

2. Take 5g of ginseng extract powder sample, dilute with water to 50ml, centrifuge, pass 5ml of methanol and 10ml of distilled water continuously through purification C18 cartridge to activate, and then adsorb 5ml of centrifuged sample solution. I order it.

3. Wash the adsorbed C18 Cartridge with 25 ml of distilled water and 10 ml of 25% methanol (2~3 ml per minute), elute with 4 ml of methanol, collect in a 5 ml flask, and dilute to the indicated line with methanol, which is used as a test solution. .

4. In addition, in order to compare the ginseng ingredient content of Panaxena Jinsan, a ginseng extract of the present invention, with the ginseng ingredient content of a red ginseng concentrate from another company already sold as a product, 5 ml of a sample of a red ginseng concentrate from another company was taken and diluted with water to make 20 ml. A comparative test solution was prepared using the same method described in paragraphs 2 and 3.

5. The detection wavelength of the UV absorbance detector was set to 203㎚ and using HPLC equipped with an octadecylsilica column, the mixing ratio of the mobile phase water and Acetonitrile was varied for each time period to determine the Ginsenosides (Rb1, Rg1, Re, The contents of each type (Rd, Rc, Rb2) were analyzed, and the result table and comparison graph are shown in Figure 1. Since the Ministry of Food and Drug Safety Notification standard for ginseng notification-type health functional food ingredient is that the sum of the contents of Ginsenoside Rg1 and Rb1 is 3 to 80 mg, looking at the result table below, the sum of the contents of Ginsenoside Rg1 and Rb1 of Panaxena Jinsan is 26.96 mg. It is judged to meet the quality standards for ginseng notification type health functional food ingredients.

Ginsenoside types Content (mg/g) Ginsenoside types Content (mg/g) Ginsenoside Rg1 8.33 Ginsenoside Re 13.32 Ginsenoside Rb1 18.63 Ginsenoside Rc 14.94 Ginsenoside Rb2 13.97 Ginsenoside Road 6.01

[Experimental Example 6] Protein content analysis evaluation of ginseng extract (Panaxana Ginsan) (crude protein analysis method - Semi micro Kjeldahl method)

1. Measure 2g of ginseng polysaccharide extract powder sample and place it in a Kjeldahl flask. Wrap the sample in parchment paper and place it in a Kjeldahl flask.

2. Add 3g of decomposition accelerator, 0.25g of titanium dioxide, and 25ml of H2SO4 (sulfuric acid) and heat.

Decomposition accelerator: 50g K2SO4 (potassium sulfate), 5g CuSO4 (copper sulfate) -> Grind -> Mix

3. Due to heating, the color of the sample solution changes from black brown to dark brown, and finally changes from blue to yellow green to transparent color. From this point, heat for 1 more hour to complete decomposition and then cool for 30 to 40 minutes.

4. Transfer the sample to a 500 ml Erlenmeyer flask, rinse the Kjeldahl flask with distilled water, and add it to the Erlenmeyer flask to make the final volume 200 ml.

5. Take 40 ml of the 200 ml of diluted sample solution, place it in a Kjeldahl flask, and add 15 ml of NaOH for neutralization.

6. Take 25 ml of 4% H3BO3 in a 300 ml beaker and add about 3 drops of mixing indicator.

The end of the cooling pipe should be submerged in 4% H3BO3 to prevent NH4 (ammonia) from escaping due to heating.

7. Turn on the coolant and heat it for a long time. The color of H3BO3 with the addition of indicator changes from pink to light green.

8. Stop heating when the total amount of NH4 coming through the cooling pipe and the total amount of H3BO3 in the beaker reaches 50 ml.

9. Titrate with 0.05-N HCl and calculate according to the formula.

<Equation>

V1: 0.05N-HCl ml consumed in the sample, V0: 0.05N-HCl ml consumed in the blank test,

F: 0.05N-HCl Factor (1.033), 14: Nitrogen conversion factor, D: Dilution factor of sample and distilled water (constant volume/collection amount) -> 200/40 = 5, S: Sample collection amount (g)

Types of Ginseng Polysaccharides Protein content (%) Ginseng polysaccharide with a molecular weight of 1,726,284 1.5 Ginseng polysaccharide with a molecular weight of 181,813 1.2 Ginseng polysaccharide with a molecular weight of 40,592 0.8

Below is a table summarizing the results of the above experiments.

Analysis items Analysis method Result value Glucose content D.N.S. Law 69.3% Molecular weight distribution G P C method Peak number 6 (Mw 2,000,000 ~ Mw3,500) area 62.53% (Mw 2,000,000 ~ Mw3,500) Constituent sugars H P L C method Mw(1,726,284) glucose 90.52% galactose 9.15% Arabinose 0.04% rhamnose 0.01% xylose 0.16% Mw(181,813) glucose 91.82% galactose 2.48% Arabinose 0.52% rhamnose 4.60% xylose - Mw(40,592) glucose 79.51% galactose 4.08% Arabinose 11.42% rhamnose 3.81% xylose - Ginseng Saponins Saturated butanol method 164.7(mg/g) Ginseng ingredients H P L C method Ginsenoside Rg1+Rb1 26.96㎎ protein Semi micro Kjeldahl method Mw(1,726,284) 1.5% Mw(181,813) 1.2% Mw(40,592) 0.8%

Claims (10)

As a ginseng extract,
The ginseng extract is based on the total weight of the extract,
At least 65% by weight of polysaccharide; And 10% by weight or more of saponin,
The polysaccharide of the ginseng extract is a ginseng extract containing polysaccharides with a molecular weight of 3,500 to 2,000,000 in an amount of 55% or more based on the total weight of the polysaccharide. According to paragraph 1,
The ginseng extract includes one or more of the following:
i) Containing polysaccharides with a molecular weight of 35,000 to 45,000 at 15 to 20% by weight based on the total weight of the polysaccharides;
ii) containing polysaccharides with a molecular weight of 160,000 to 200,000 in an amount of 17 to 23% by weight relative to the total weight of the polysaccharides;
iii) containing a polysaccharide with a molecular weight of 1,500,000 to 2,000,000 at 0.5 to 1.5% by weight based on the total weight of the polysaccharide;
iv) Polysaccharides with a molecular weight of 35,000 to 45,000 contain more than 70% by weight of glucose or less than 3% by weight of protein relative to the molecular weight of the polysaccharide;
v) Polysaccharides with a molecular weight of 160,000 to 200,000 contain more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide; and
vi) Polysaccharides with a molecular weight of 1,500,000 to 2,000,000 contain more than 80% by weight of glucose or less than 2% by weight of protein relative to the molecular weight of the polysaccharide. According to paragraph 1,
Included in the ginseng extract,
A ginseng extract in which the total content of ginsenosides Rg1 and Gb1 is 2.5% by weight or more based on the total weight of the extract. A food composition comprising the ginseng extract of any one of claims 1 to 3. A method for producing the ginseng extract of any one of claims 1 to 3,
The method is a method of producing a ginseng extract, comprising the step of extracting ginseng. According to clause 5,
The step of extracting the ginseng is,
Ginseng whole plant extraction step; And a method for producing a ginseng extract, comprising a ginseng foil extraction step. According to clause 6,
The ginseng whole plant extraction step and the ginseng foil extraction step include being performed by solvent extraction,
The solvent used in the ginseng whole plant extraction step includes water,
The solvent used in the ginseng foil extraction step includes one or more of an organic solvent and an aqueous solution thereof. According to clause 6,
The ginseng whole plant extraction step is performed under one or more of the following conditions, a method for producing a ginseng extract:
i) Extraction temperature: 80~95℃;
ii) Extraction time: 6 to 12 hours; and
iii) Extraction solvent volume: 3 to 8 times the weight of ginseng whole plant. According to clause 6,
The ginseng foil extraction step is a method for producing ginseng extract, which is performed under one or more of the following conditions:
i) Extraction temperature: 50℃ or less;
ii) Concentration of extraction solvent: 60~90% (v/v); and
iii) Extraction time: 3 to 6 hours. According to clause 6,
The ginseng foil extraction step includes extracting the ginseng foil primarily extracted through the ginseng whole plant extraction step,
The method for producing the ginseng extract further comprises the step of mixing the extract obtained from the ginseng whole plant extraction step and the extract obtained from the ginseng foil extraction step.