KR20240048595A - Feed for companion animals with enhanced antibacterial properties and method for manufacturing the same - Google Patents

Abstract

The present invention provides a companion animal feed that has an excellent balance of animal and plant proteins, an excellent balance of unsaturated and saturated fatty acids, excellent anti-inflammatory and antibacterial properties, excellent immunity-enhancing effects and antioxidant effects, and excellent storage stability, and its production. It is a method-related technology.
In the method of manufacturing pet food, the first step is to manufacture a low-protein mixture fermented mainly from rice flour, and the second step is to manufacture animal and plant complex proteins that also involve a fermentation process by adding salted seafood to wheat bran. And the animal and plant complex proteins obtained in the second step are mixed with coconut oil, turmeric, and a low-protein mixture to obtain an antibacterial complex mixture, to which astragalus powder, eucalyptus leaf extract, and arctic stork grass extract are added, and process water is mixed to produce feed. After obtaining a paste of the composition, steaming the feed raw material composition, extrusion molding at 140 to 170 ° C. at 3 to 10 kg / ㎤ to pelletize, and performing a drying process so that the moisture content of the pellets is less than 10% by weight. This is a technology for pet food with enhanced antibacterial properties.

Description

Feed for companion animals with enhanced antibacterial properties and method for manufacturing the same}

The present invention relates to pet food with increased antibacterial properties and a method for manufacturing the same. More specifically, it has an excellent balance of animal and plant proteins, an excellent balance of unsaturated and saturated fatty acids, excellent anti-inflammatory and antibacterial properties, and excellent immunity. This invention was started with the task of providing pet food with enhancing and antioxidant effects and excellent storage stability.

Recently, as the number of companion animals such as dogs and cats has increased worldwide, diseases similar to human adult diseases such as obesity, diabetes, and liver disease have increased due to aging, lack of exercise, and overnutrition of companion animals.

In this regard, many feeds have been developed to prevent obesity and weight gain in companion animals. For example, feeds containing a large amount of oil containing a large amount of unsaturated fatty acids that exhibit various physiological activities have been proposed.

For example, in Registered Patent No. 10-1834524, the method for manufacturing nutritional feed for companion animals includes the steps of (a) ripening a dough of wild greens powder mixed with purified water at a low temperature and then manufacturing a round-shaped aged wild greens pill; b) Mix 80 to 120 parts by weight of salmon and 30 to 45 parts by weight of sardines with 100 parts by weight of salmon, stir for 60 to 75 minutes, and then extrude and cut into a cylindrical shape with a diameter of 5 to 10 mm and a height of 5 to 10 mm at 100 to 120 ° C. A method of manufacturing nutritional feed for companion animals comprising the steps of manufacturing processed fish meat pieces by rapidly cooling them to 40 to 43°C; and (c) press-injecting 2 to 5 ripened wild greens rings into the outer periphery of the processed fish meat pieces. This is presented.

Another patented technology, registered patent number 10-2029027, is a feed additive composed of a basic composition containing crude protein containing insect powder made from insect larvae, crude fiber, calcium and phosphorus, and nutrients that are not synthesized in the body. The insect powder is composed of 7 to 12% of the basic composition weight of oil obtained by defatting 36% of the larvae, the crude fiber is 5% by weight, and the calcium is 0.7% by weight, A pet food using insect powder mixed with phosphorus at a weight ratio of 0.5% is proposed.

However, feed containing a large amount of the above-mentioned unsaturated fatty acids is easily oxidized depending on the storage environment, affecting odor and palatability, and when nutrients or mineral components are mixed with oil containing a large amount of unsaturated fatty acids, the oxidation reaction of the oil is promoted. The problem of further deterioration in preservation stability has been raised.

In addition, recently, conventionally provided pet food is provided as a high-protein food centered on meat or fish, and pets that eat high-protein food cannot stay in the intestines for long and pass down quickly, resulting in moist stools, if not diarrhea, and as a result, digestion problems. Because it happens too quickly, the pet is always hungry, and if provided with a large amount of high-protein feed, it develops into diarrhea, so there has been a recurring problem that the amount of feed cannot be increased much.

As a result of research to solve these problems, the inventors of the present invention produced a complex of omega-3 fatty acid oil and protein containing a large amount of unsaturated fatty acids, chicken milk containing a large amount of saturated fatty acids, and a complex containing an appropriate amount of animal and vegetable protein. This is mixed with vegetable oil while stirring slowly to produce a composite with an appropriate fat and low protein structure, and then mixed with grains, beet pulp, etc. and pelletized to produce feed, which increases the pet's immunity and improves preservation stability. By confirming the embodiment, the present invention was completed.

(Patent Document 1) Republic of Korea Patent No. 10-2029027

The present invention provides a companion animal feed that has an excellent balance of animal and plant proteins, an excellent balance of unsaturated and saturated fatty acids, excellent anti-inflammatory and antibacterial properties, excellent immunity-enhancing effects and antioxidant effects, and excellent storage stability, and its production. The purpose is to provide a method

Humans have been using fermentation as a method of storing food and making food for thousands of years. At our center, we are also using rice powder as a manufacturing method for pet food to increase antibacterial properties and palatability. Safe storage of finished feed through the first step of introducing a fermentation method that adds malt to the low-protein mixture manufacturing process and the second step of manufacturing animal and plant complex proteins that also involve a fermentation process by adding salted seafood to wheat bran. It is applied to increase the antibacterial complex mixture by mixing the animal and plant complex proteins obtained in the second step with coconut oil, turmeric, and a low-protein mixture, and to this, astragalus powder, eucalyptus leaf extract, and arctic stork grass extract are added and processed. Pet food with enhanced antibacterial properties obtained by mixing water to obtain a paste of the feed composition, steaming the feed raw material composition, extrusion molding into pellets, and performing a drying process so that the moisture content of the pellets is less than 10% by weight. It is an invention of technological thought that aims to achieve.

Protein ingredients are perishable, so in order to store them for a long time, they are fermented by the food's own enzymes, lactic acid bacteria, Bacillus, yeast, etc., and a large amount of ammonia is produced due to protein fermentation. This occurs, the concentration increases, halophilic bacteria proliferate, and lactic acid produced by lactic acid bacteria inhibits the growth of other harmful bacteria, preventing spoilage and enabling long-term preservation, in order to apply this principle to pet food. did.

More specifically, based on 100 parts by weight of rice powder, 1 to 3 parts by weight of malt, 5 to 15 parts by weight of krill oil, 5 to 20 parts by weight of Spirulina, 10 to 30 parts by weight of sardine oil, and 120 to 240 parts by weight of process water. A first step process of producing a low-protein mixture by mixing the parts and fermenting them at 30 ± 5 ℃ for more than 72 hours, and 50 to 100 parts by weight of meat powder, 5 to 10 parts by weight of salted seafood, and 10 parts by weight of asparagus for 100 parts by weight of wheat bran. 30 parts by weight, 10 to 30 parts by weight of sea buckthorn leaf powder, 20 to 30 parts by weight of fish protein hydrolyzate, 30 to 50 parts by weight of chicken oil, and 120 to 240 parts by weight of process water are mixed and fermented for more than 72 hours to produce an animal-plant complex. The second step process of producing protein, 5 to 15 parts by weight of coconut oil, 5 to 15 parts by weight of turmeric, and 40 parts by weight of the low protein mixture obtained in the first step, based on 100 parts by weight of animal and plant complex protein obtained in the second step. A third step process of obtaining an antibacterial complex mixture by mixing 60 to 60 parts by weight, and 20 to 30 parts by weight of astragalus powder, eucalyptus leaf extract, and Arctic stork herb extract 5:5 per 100 parts by weight of the composite mixture obtained in the third step. 10 to 20 parts by weight of the mixed extract mixed in a weight ratio is added and 60 to 90 parts by weight of process water is mixed to obtain a feed composition in the form of a dough in the fourth step, and the feed raw material composition in the form of a dough obtained in the fourth step is mixed with 90 to 130 parts by weight. The fifth step is to steam at ℃ for 10 to 30 minutes, extrude at 140 to 170℃ at 3 to 10 kg/cm3 to form pellets, and perform a drying process so that the moisture content of the pellets is less than 10% by weight. It is characterized by a manufacturing method of pet food comprising:

In the method of manufacturing pet food herein, the salted seafood can be selected from salted shrimp, salted clams, salted clams, salted anchovies, salted pollack roe, and salted pollack roe (pollock intestines), and can be used in the fourth step. Process water can be applied to use a water-dispersed nano-sulfur solution to increase antibacterial activity.

In addition, in the manufacturing method of pet food of the present invention, 0.5 to 5 parts by weight of a functional material selected from beet pulp, sugar, mineral mix, and vitamin mix is added in the fourth step process to provide a technical idea that can be applied to be used as a palatable feed. Includes.

In addition, the present invention provides pet food with enhanced antibacterial properties prepared by the above method.

The pet food of the present invention provides excellent animal and plant protein balance, excellent unsaturated and saturated fatty acid balance, excellent anti-inflammatory and antibacterial effects, excellent immunity enhancing effect and antioxidant effect, and provides excellent storage stability.

The present invention will be described in detail below.

The present invention relates to a method for manufacturing pet food that secures antibacterial properties, and includes 1 to 3 parts by weight of malt, 5 to 15 parts by weight of krill oil, 5 to 20 parts by weight of Spirulina, per 100 parts by weight of rice powder, The first step of producing a low-protein mixture by mixing 10 to 30 parts by weight of sardine oil and 120 to 240 parts by weight of process water and fermenting at 35 ± 5 ℃ for more than 72 hours, and 50 to 100 parts by weight of meat powder per 100 parts by weight of wheat bran. parts by weight, 5 to 10 parts by weight of salted seafood, 10 to 30 parts by weight of asparagus, 10 to 30 parts by weight of buckthorn leaf powder, 20 to 30 parts by weight of fish protein hydrolyzate, 30 to 50 parts by weight of chicken oil, and 120 to 240 parts by weight of process water. A second step process of producing animal and plant complex proteins by mixing parts by weight and fermenting for more than 72 hours, and 5 to 15 parts by weight of coconut oil and 5 to 15 parts by weight of turmeric for 100 parts by weight of animal and plant complex proteins obtained in the second step. A third step process of obtaining an antibacterial complex mixture by mixing 40 to 60 parts by weight of the low-protein mixture obtained in the first step and 20 to 30 parts by weight of astragalus powder and eucalyptus based on 100 parts by weight of the complex mixture obtained in the third step. 10 to 20 parts by weight of a mixed extract of leaf extract and Arctic stork grass extract mixed at a weight ratio of 5:5 is added and 60 to 90 parts by weight of process water is mixed to obtain a feed composition in a dough state, and in the fourth step. The obtained dough-like feed raw material composition is steamed at 90 to 130°C for 10 to 30 minutes, then extruded at 140 to 170°C at 3 to 10 kg/cm3 to form pellets, and the moisture content of the pellets is less than 10% by weight. It is characterized by a manufacturing method of pet food that includes a fifth step of performing a drying process to achieve this.

The rice used in the process to obtain a low-protein mixture in our pet food manufacturing method has a lower protein content than other grains, but the content of lysine, an essential amino acid in amino acid distribution, is about twice that of corn, millet, and wheat flour. More than 75% of the fatty acids in rice are unsaturated fatty acids, with very little saturated fatty acids, and are rich in vitamin B complexes such as vitamins B1 (thiamin) and B2 (riboflavin), as well as vitamins such as vitamin E, folic acid, and niacin. It contains several important vitamins.

In addition, wheat bran, which is used to obtain complex animal and plant proteins, refers to the residue left after wheat has been sucked to remove the powder. Compared to other grains, wheat has a soft endosperm and a hard outer husk, so it is in powder form rather than grain form like rice or barley. Since it is advantageous to mill it, when the outer husk is removed, it breaks easily and becomes flour. Gluten protein, the main component of wheat protein, forms gluten when in contact with water and has viscoelasticity, so it is desirable to use wheat ingredients as feed. It is preferable to use wheat bran rather than wheat flour. Wheat bran has a large volume and low calories, but contains many minerals, so it is highly palatable as feed and is easily digested.

However, in the present invention, rice flour and wheat bran are used as basic raw materials to achieve a balance of animal and plant proteins, but it was confirmed that partial spoilage occurs during distribution and storage of feed, and as a means to prevent this, rice flour was added with malt. By adding a small amount of salted fish to the bran and using fermented raw materials that had undergone a fermentation process for a certain period of time, we wanted to extend the storage period of the finished product and achieve the effect of providing it as a favorite food for pets.

In addition, the krill oil used in the process to obtain our low-protein mixture not only has a bioabsorption rate that is 1.6 to 4 times higher than that of regular fish oil, but can also increase HDL by 43.5% and reduce LDL by 34%. It was applied to be included in pet food, and Spirulina contains 65-70% protein, 6-9% lipid, 8-14% carbohydrate, 4-8% dietary fiber, 13 types of minerals, 12 types of vitamins, It has various effective nutrients necessary for the human body, including three types of pigments, and when viewed in terms of its composition ratio, it can be said to be close to a complete food. In particular, the protein is composed of more than 18 types of amino acids.

The spirulina is a high-protein food that contains more protein (69.5%) than chlorella (50%), which is famous for its high protein content, and contains all essential amino acids in a balanced manner.

In addition, sardines have been an excellent food for many marine life since ancient times due to the protein and fat content in their bodies compared to their small size, and have been called the rice of the sea. Sardine oil made by pressing them contains a balanced amount of excellent proteins and essential amino acids. It was intended to be used as a protein source.

The meat used to obtain animal complex protein in our clinic can be one or more types of meat selected from chicken, duck, venison, beef, and pork, frozen, powdered, or cut into pieces. In particular, chicken breast has a high Since it is not popular as a protein source or because it is too poor for human consumption, it was intended to be used as an animal protein source for animal feed. There are two types of asparagus (Asparagus officinalis) used to obtain plant protein, green asparagus and white asparagus, which have nutritional value. There is a slight difference in that asparagus contains a large amount of alkaloids such as asparagin and asparaginic acid as special ingredients, as well as minerals such as calcium, phosphorus, and potassium, and vitamins A, B1, B2, C, etc. Asparagine, the main ingredient of asparagus, is known to be effective in treating neuralgia and rheumatism caused by uric acid accumulation by helping kidney function, promoting uric acid excretion, and destroying oxalic acid crystals in the kidneys and entire muscle system. Asparagus is edible and medicinal. It is a vegetable species whose roots and shoots have been used since ancient times as a diuretic and sedative.

In addition, the sea buckthorn tree grows in the Himalayas, China, Russia, North America, India, and cold desert regions, but it is a plant that is highly adaptable and can grow at altitudes and high temperatures. It is rich in vitamin C and tocopherol, and is used at our center to provide antioxidant effects. Use linden tree leaf powder.

The coconut oil used to obtain the antibacterial complex mixture in our clinic is extracted from copra, the inner bark of the coco palm fruit. 90% of the fat is saturated fatty acid, and the main components are lauric acid (44-52%) and decanoic acid (6-10%). %), linoleic acid (2.5-12%), and myristic acid (4-16%), and contains components such as stearic acid, arachnic acid, octanoic acid, oleic acid, palmitic acid, hexadecenoic acid, nucleic acid, iron, and phosphorus. It contains a substance that forms an oil film on the pet's hair, maintaining the protective layer for a long time, and has a high affinity for hair lipids, providing nutrients to the hair. It is an oil with high antibacterial and antioxidant properties among natural oils, and has high molecular weight. Due to the short-structured medium-chain fatty acid properties, it is easily absorbed into the skin and is used to provide a therapeutic effect for skin wounds and troubles.

Turmeric is also a traditional medicinal plant used for food and medicinal purposes in tropical countries such as Indonesia and Malaysia to treat hepatitis, liver disease, stomach disease, rheumatism and skin inflammation. Turmeric contains bioactive compounds of curcuminoids, camphor, geranyl acetate, zerumbone, β-curcumene, zingiberene, ar-curcumene, and xanthorrhizol, of which xanthorrhizol has antibacterial, antifungal, anticancer, phytoestrogen, and neuroprotective properties. It is reported to have various effects, including activity.

In addition, Astragalus used in the fourth step of the process herein is the peeled root of Astragalus membranaceus Bunge and contains saponins, flavones, and polysaccharides as bioactive ingredients. The polysaccharides include Astragalan I and II, which are beta-glucan substances. , and Ⅲ, and astragalus is known to have immune-enhancing and antibacterial effects due to the presence of beta-glucan substances (Zhu, YouPing, Chinese Materia Medica, Harwood Academic Publisher, p 560, 1988).

Astragalus was intended to be used in conjunction with the antibacterial active ingredients contained in the feed ingredients of our institution to greatly reduce the possibility of spoilage of the feed composition and to provide benefits to the health of companion animals. In addition, eucalyptus leaf extract and arctic stork grass extract are used together. When used, eucalyptus leaf extract is extracted with ethanol after chopping the leaves. The extraction process can be performed in the range of 60 ± 5℃. After obtaining the extract from eucalyptus leaves, it is filtered, concentrated, and dried. Bar, eucalyptus leaf extract has excellent antibacterial effect and has been confirmed to increase antioxidant effect.

In addition, Eriophorum scheuchzeri ssp. arcticum is a grass that is often seen in Arctic tundra wetlands. The extract of Arctic stork, which has seeds that look like cotton, can also be used as an extract extracted from the white cotton seeds. When extracting the grass extract from white cotton, which is a dried seed, with ethanol, the extraction can be performed in the range of 55 ± 5℃. After obtaining the extract from Arctic Storkweed, filtering, concentrating, and drying it to obtain Arctic Storkweed extract. When used, it was confirmed that the extract of Arctic stork grass has excellent antibacterial effect and also increases antioxidant effect.

In particular, it was confirmed that a synergistic effect in antibacterial activity was obtained when a mixed extract of the eucalyptus leaf extract and the Arctic stork grass extract mixed half and half at a weight ratio of 5:5 was used together rather than each component.

In addition, in the fourth step process of the present application, functional substances selected from saccharides, mineral mixes, and vitamin mixes can be added in the range of 0.5 to 5 parts by weight, and the sugars include fructooligosaccharides, isomaltooligosaccharides, galactooligosaccharides, and maltooligosaccharides. , one or more types selected from xylooligosaccharides, gentio-oligosaccharides, and agave syrup may be used, and the mineral mix is one type selected from calcium, phosphorus, sodium, potassium, chlorine, sulfur, and magnesium, which are required in large quantities in the body. It can be manufactured by mixing the above microminerals with trace amounts of one or more microminerals selected from iron, zinc, iodine, copper, selenium, manganese, cobalt, chromium, fluorine, and molybdenum. The minerals are stored in bone tissue, It forms the framework of soft tissues, maintains acid-base balance, activates enzymes, and is involved in maintaining osmotic pressure in the body.

In addition, the vitamin mix may be manufactured by mixing vitamin C, vitamin K, and niacin (vitamin B group), which can be synthesized in the body, as well as the remaining 13 types of vitamins that must be consumed through food. Vitamins are consumed as a non-energy source. It can be selectively added and used to help enzymatic reactions that allow food to be absorbed as nutrients, or to help perform various physiological functions such as DNA synthesis, bone development, cell membrane formation, blood coagulation, and nerve signal transmission. .

Hereinafter, the present invention will be described in more detail through manufacturing examples and examples based on the technical ideas of the present application. However, the following examples are intended to explain the present invention in more detail, and the scope of the present invention is limited to the following examples. It is to be understood that the examples are not limited and that the following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.

Raw material preparation example 1: Preparation of eucalyptus leaf extract

1 kg of eucalyptus leaves were cut into small pieces, added to 2 L of ethanol (concentration 40% (v/v)), and extracted while stirring at 55 to 60°C for 4 hours. The extract was filtered under reduced pressure using a pressure reduction pump and Whatman No. 2 filter paper, and the filtered extract was concentrated using a vacuum rotary concentrator and then spray-dried to obtain a eucalyptus leaf extract in powder form.

Raw material preparation example 2: Preparation of Arctic stork grass extract

1 kg of dried Arctic stork grass cotton was cut into small pieces, added to 2 L of ethanol (concentration 40% (v/v)), and extracted while stirring at 50 to 55°C for 4 hours. The extract was filtered under reduced pressure using a pressure reduction pump and Whatman No. 2 filter paper, and the filtered extract was concentrated using a vacuum rotary concentrator and then spray-dried to obtain a powdered extract of Arctic dorado.

Raw material preparation example 3: Water-dispersed nano sulfur production example

Weigh and add 650 g of water (general tap water), 10 g of surfactant for dispersion, and 20 g of water-soluble polymer in order into a stirring tank to make a homogeneous solution. Legal sulfur powder 30 is provided as a fine powder in the 320 mesh range. After adding and dispersing g, the dispersion mixed solution was transferred to a dyno-mill device and milled for 10 hours at room temperature to pulverize the sulfur particles into 200 nm (nano) particle size to obtain 710 g of water-dispersed nano sulfur. The water-dispersed nano-sulfur can be obtained as an emulsion-like water-dispersed nano-sulfur solution with a solid content of 34%, a pH of 6.5, and a white appearance. This allows the water-dispersed nano-sulfur solution to be used as process water in the fourth step of the process. The aqueous nano-sulfur solution in an emulsion state was diluted with 20 times the amount of water and used as process water in the fourth step of the process.

Example 1: Preparation of pet food

In the first step, add 3g of malt, 10g of krill oil, 15g of spirulina, and 15g of sardine oil to 100g of rice powder, mix by injecting 200 cc of process water while stirring at 2000-3000 rpm, and leave for more than 72 hours at 35±5℃. The first step was to produce a low-protein mixture by fermentation.

In the second step process, to obtain animal and plant complex proteins, 100g of wheat bran, 80g of chicken breast powder, 5g of salted clams, 20 parts by weight of asparagus powder, 20g of buckthorn leaf powder, 25g of fish protein hydrolyzate, and 40g of chicken oil were added and 2000g was added. The second step process of producing complex animal and plant proteins was performed by injecting/mixing 200 cc of process water and fermenting for more than 72 hours while stirring at -3000 rpm.

Looking at the second step process in more detail, wheat bran has a very high digestibility and absorption rate of about 90-95%, has a very low allergy rate, and is rich in fiber, minerals, and vitamin C, and chicken breast is a high protein source but good for human consumption. Since it was not popular because it was too harsh, it was intended to be used as an animal protein source for animal feed.

In addition, if animals consume little fat, they may experience dry skin, hair loss, and poor coat quality, and may also experience problems with their immune system. It is also bad for the heart and joints, so fat must be included in animal feed, so chicken milk is essential. and fish protein hydrolyzate are preferably included, and the fish protein hydrolyzate is hydrolyzed to a size of less than 5,000 daltons (the size of one oxygen molecule is about 16 daltons, and one carbon molecule is about 12 daltons) so that the immune system does not recognize it. We wanted to obtain the effect of suppressing dietary allergies as a protein.

In addition, animals that are too young or at an advanced age have relatively low digestibility, so feeding them finely decomposed protein in advance can increase digestion and absorption. Lastly, when enzymatic digestion is used among various hydrolysis methods, the flavor of the raw materials is reduced. The goal was to achieve the effect of enhancing the quality and preserving it for a long period of time.

In the present invention, among the fish protein hydrolyzates, salmon protein hydrolyzate is preferred. Salmon protein hydrolyzate is a functional raw material with proven effectiveness, commonly referred to as "hydrolyzed salmon protein," and vitamin A contained in salmon reduces allergic reactions. It is effective in preventing or treating eye diseases and is also desirable because it provides nutrients to the skin and hair and makes rough skin shiny.

The salmon protein hydrolyzate or chicken oil contains a large amount of unsaturated fatty acids such as omega-3 fatty acids (hereinafter referred to as 'omega-3 fatty acid oil') and is included in feed because it has a significant impact on the healthy skin and coat of animals. However, if it is contained in more than the necessary amount, it is likely to become rancid during storage or distribution, and when the unsaturated fatty acid oil becomes rancid, an unpleasant odor is generated and the palatability of pets is reduced. Therefore, for 100 parts by weight of wheat bran, the fish protein hydrolyzate should be 20%. It was preferable to use chicken oil in the range of 40 to 60 parts by weight based on 100 parts by weight of wheat bran.

In the third stage of the process, in order to increase the anti-inflammatory and antibacterial properties of 100g of the animal and plant complex protein prepared in the second stage, 10g of coconut oil, 10g of turmeric, and 60g of the low protein mixture obtained in the first stage were mixed at 100-1000 rpm. The third step process was performed by stirring.

In order to increase immunity to the animal and plant complex protein prepared in the second step as described above, coconut oil and turmeric are added and mixed with the low-protein mixture prepared in the first step while stirring at a low speed of 100-1000 rpm. , it not only blocks odors that reduce animal palatability, such as the fishy smell of sardine oil contained in the low-protein mixture prepared in step 1, but also prevents unsaturated fatty acids from easily becoming rancid and has the effect of increasing immunity. will be provided.

In the fourth step process herein, 25 g of astragalus powder, eucalyptus leaf extract, and arctic stork grass extract prepared from the above raw materials are mixed in equal amounts with respect to 100 g of the complex mixture obtained in the third step, and 80 parts by weight of process water is mixed/stirred with 40 g. A feed composition in the form of dough was obtained.

In the 5th step process herein, the dough-like feed composition obtained in step 4 is steamed at 110°C for 20 minutes, then extruded at 160±5°C and 5 kg/cm3 to pelletize into 0.8 cm size. After drying for 60 minutes in a drying furnace maintained at 110°C, pet food with a pellet moisture content of 8.20% by weight was obtained.

Example 2: Preparation of pet food

The process was carried out in accordance with Example 1, but in the fourth step, the process water was used as ordinary tap water, but in Example 2, the water-dispersed nano-sulfur solution in the emulsion state obtained in the water-dispersed nano-sulfur preparation example obtained in Raw Material Preparation Example 3 was used. An aqueous nano-sulfur solution diluted 20 times with water is used as process water, and 2g of sugars, 2g of mineral mix, and 2g of vitamin mix are added and applied in the first, second, third, and fifth steps. Feed was prepared in the same way.

Comparative Example 1: Preparation of pet food (conventional manufacturing method)

(1) 10g salmon oil, 4g krill oil, 50g krill powder, 60g pea protein, 150g corn gluten, 100g organic chicken powder, 40g salmon protein hydrolyzate, 72.5g chicken oil, 30g sunflower seed oil, 490g grain powder, beet walnuts Dough was prepared by mixing 60g, 3g of yucca extract, and 200g of water.

(2) The dough was steamed at 100°C for 20 minutes and then extruded at 150°C at 5 kg/cm3 to pelletize. At this time, the size of the pellets produced was set to an average diameter of 0.8 cm.

(3) Then, pet food with a moisture content of 8% by weight was prepared by drying the pellets at 110°C for 60 minutes.

Test Example 1: Safety confirmation

The experimental animals were 4-week-old Sprague-Dawley male rats (Central Lab animal, Seoul, Korea) fed pellet-type solid feed (Jeil animal feed) in an environment-controlled breeding room (temperature 20°C, humidity 55%, 12-hour dark/light cycle). Co., Daejeon, Korea) for two weeks and grew to an average weight of about 200 g. Toxicity tests were conducted on the eucalyptus leaf ethanol extract (Preparation Example 1) and the Arctic stork grass ethanol extract (Preparation Example 2). It was carried out.

Specifically, 5 mg of the eucalyptus ethanol extract or Arctic stork grass ethanol extract was administered to 5 each of the 4-week-old rats (2 groups, 10 rats in total) as pellet-type solid feed (Jeil animal feed Co., Daejeon, Korea). ) was fed for 4 weeks and toxicity was evaluated.

As a result of the toxicity evaluation, it was confirmed that none of the mice participating in the experiment died, and all 10 mice remained healthy for 4 weeks.

Test Example 2: Measurement of antibacterial activity

The antibacterial activity of eucalyptus ethanol extract (prepared in Comparative Example 1) or Arctic stork grass ethanol extract (prepared in Comparative Example 2) and their mixtures (1:1 weight ratio) were evaluated by the following method.

Representative food poisoning bacteria (Bacillus cereus, Bacillus subtilis, Campylobacter jejuni, Escherichia coli, Escherichia coli O-157, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, Salmonella thypimurium, Vibrio parahaemolyticus, Yersinia enterocolitica) were used.

The above bacteria were inoculated with 50 μl of the culture in 10 ml of sterilized TSB medium and subcultured at 30°C for 24 ± 1 hours. The concentration of this culture was maintained at Abs 0.6 ± 0.1 at the microplate reader absorption wavelength of 655 nm. The subcultured experimental bacteria were diluted 10-3 times with sterilized distilled water and subjected to disk diffusion method.

That is, the diluted strain was placed on a sterilized cotton swab, spread and smeared on a 4 mm thick plate medium, placed on a sterilized 8 mm (1 mm thick) filter paper, and the eucalyptus ethanol extract (prepared in Preparation Example 1) and the ethanol extract of Arctic stork (prepared in Preparation Example 2) were added. and their mixture (1:1 weight ratio) were each dissolved in 50 ㎍ water and injected. After sufficiently inhaling for about 20 minutes, the culture was incubated for 30 to 24 ± 1 hours and growth rings were confirmed.

The antibacterial activity was evaluated by measuring the size of the transparent zone including the filter paper, and the results are shown in Table 1 below.

Strain name strain number ^a Antibacterial activity (mm) ^b Eucalyptus ethanol extract (Preparation Example 1) Arctic stork grass ethanol extract (preparation example 2) Eucalyptus and arctic storkgrass mixture (1:1 weight ratio)
bacteria Bacillum cereus KCTC 1012 15 13 16 Bacillus subtilis KCCM 1021 14 15 17 Campylobacter jejuni KCCM 41772 14 15 16 Escherichia coli ATCC 25922 15 16 17 Escherichia coli 0-157 ATCC 43888 15 14 16 Listeria monocytogenes ATCC 15313 15 14 16 Pseudomons aeruginosa KCTC 1750 16 14 17 Staphylococcus aureus ATCC 25923 14 15 16 Salmonella enteritidis KCCM 11862 14 16 16 Salmonella thypimurium ATCC 13076 14 16 17 Vibrio parahaemolyticus ATCC 17802 16 15 17 Yersinia enterocolitica ATCC 23715 14 16 17

Co., Ltd. a: ATCC American Type Culture Collection, KCTC Korea Research Institute of Bioscience and Biotechnology, Biological Resources Center; KCCM Korea Seed Culture Association

b: Size of transparent band (diameter mm)

As can be seen in Table 1, eucalyptus ethanol extract (prepared in Preparation Example 1), Arctic stork grass ethanol extract (prepared in Preparation Example 2), and their mixtures (1:1 weight ratio) all have excellent antibacterial activity against all bacteria. indicated.

In particular, the mixture showed better antibacterial activity.

Test Example 3. Antioxidant activity of feed

The results of the analysis of antioxidant efficacy of pet food according to Examples and Comparative Examples are shown in Table 2 below. After storing the food at room temperature for 6 months, the antioxidant activity over time was analyzed and shown in Table 2. It was.

The method for measuring the antioxidant activity is as follows.

To determine the antioxidant effect of the above feed, 5 g of ground pet food was mixed with 20 ml of 70% ethanol, stirred using a shaker for 10 seconds, left for 30 minutes, centrifuged at 3600 rpm for 30 minutes, and filtered using filter paper. After filtering, 0.4 ml of the filtrate and 1 ml of a solution diluted with DPPH 1.5 Х 10-4M were mixed at room temperature using a shaker for 10 seconds, reacted in the dark for 30 minutes, and the absorbance was measured at 517 nm and calculated as the ratio of absorbance. did.

DPPH radical scavenging ability (%) = ((Absorbance of the no-added group - Absorbance of the sample-added group)/Absorbance of the no-added group) Х 100

division DPPH radical scavenging ability (%)
(immediately after manufacturing) DPPH radical scavenging ability (%)
(6 months after manufacturing) Example 1 18.13 19.05 Example 2 19.20 20.56 Comparative Example 1 18.02 12.25

Test Example 4. Inhibition of bacterial growth during storage period

We attempted to evaluate microbial stability by measuring the change in total number of bacteria during storage at room temperature, which is shown in Table 3 below.

division 1 day after manufacturing 1 month after manufacturing 3 months after manufacturing 6 months after manufacturing Example 1 Not detected Not detected Not detected 0.7×10 ² Example 2 Not detected Not detected Not detected Not detected Comparative Example 1 Not detected 0.6×10 ³ 066×10 ⁴ 26.8×10 ⁴

Palatability evaluation test.

70 g of each pet food of Example 2 and Comparative Example was simultaneously supplied to 20 healthy pet dogs aged 2 to 10 years, and a preference evaluation was conducted.

The palatability of the snack was rated on a 9-point scale (9 = I like it a lot; 8 = I like it; 7 = I like it; 6 = I like it a little; 5 = neither like it nor dislike it; 4 = I dislike it a little; 3 = I dislike it; A preference test was conducted according to the criteria (2=dislike; 1=dislike very much), and statistical processing was performed using the SPSS 12.0 statistical program at a significance level of p<0.05 using a multiple range test (DMR-test; Duncan multiple range test). Significance was verified.

The evaluation of palatability was evaluated after direct observation by the owner of the pet dog and is shown in Table 4 below.

At this time, the palatability evaluation value is calculated by dividing the total score given by each pet dog's owner by the number of owners and rounding it to two decimal places. The higher the value, the better the palatability.

division Example 2
(After manufacturing) Comparative example
(After manufacturing) Example 2
(After 6 months storage) Comparative example
(After 6 months storage) preference 8.8 8.4 8.4 5.3

As shown in Table 4, it was confirmed that the preference of the pet food according to the present invention was higher and the storage stability was improved compared to the feed of the comparative example.

Through the above description, the inventor of the present application attempted to explain the features of the technical idea of the present application in the preferred embodiments mentioned above, but the description was centered on the fact that the inventor of the present application confirmed the desired result through numerous trial and error processes to achieve the present invention. Of course, various modifications and variations will be possible by using similar functional raw materials as the gist of .

In addition, through the above experimental results, the present invention, when applied as pet food, maintains an excellent balance of animal and plant proteins, promotes an excellent balance of unsaturated and saturated fatty acids, and secures excellent antibacterial activity, thereby increasing storage stability. was confirmed and a patent application was filed.

Claims (5)

In the manufacturing method of pet food
35 parts by weight by mixing 1 to 3 parts by weight of malt, 5 to 15 parts by weight of krill oil, 5 to 20 parts by weight of Spirulina, 10 to 30 parts by weight of sardine oil, and 120 to 240 parts by weight of process water with respect to 100 parts by weight of rice powder. The first step of producing a low-protein mixture by fermenting at ±5℃ for more than 72 hours;
Based on 100 parts by weight of wheat bran, 50 to 100 parts by weight of meat powder, 5 to 10 parts by weight of salted seafood, 10 to 30 parts by weight of asparagus, 10 to 30 parts by weight of sea buckthorn leaf powder, 20 to 30 parts by weight of fish protein hydrolyzate, and chicken oil. A second step of producing complex animal and plant proteins by mixing 30 to 50 parts by weight with 120 to 240 parts by weight of process water and fermenting for more than 72 hours;
An antibacterial complex mixture is prepared by mixing 5 to 15 parts by weight of coconut oil, 5 to 15 parts by weight of turmeric, and 40 to 60 parts by weight of the low-protein mixture obtained in the first step with 100 parts by weight of the animal and plant complex protein obtained in the second step. The third stage of obtaining;
For 100 parts by weight of the complex mixture obtained in the third step, 20 to 30 parts by weight of Astragalus powder, 10 to 20 parts by weight of a mixed extract in which eucalyptus leaf extract and Arctic stork grass extract are mixed in a 5:5 weight ratio are added, and 60 to 60 parts by weight of process water are added. A fourth step where 90 parts by weight are mixed to obtain a dough-like feed composition; and
The dough-like feed raw material composition obtained in the fourth step is steamed at 90 to 130°C for 10 to 30 minutes, then extruded into pellets at 140 to 170°C at 3 to 10 kg/cm3, and the moisture content of the pellets is adjusted. A fifth step of performing a drying process to reduce the concentration to less than 10% by weight; A method of manufacturing pet food comprising the process. According to paragraph 1,
A method of manufacturing pet food, characterized in that the salted seafood is selected from salted shrimp, salted clams, salted clams, salted anchovies, salted pollack roe, and salted pollack roe (pollock intestines). According to paragraph 1,
A method for producing pet food, characterized in that the process water in the fourth step is applied so that an aqueous nano-sulfur solution is used. According to paragraph 1,
A method of manufacturing pet food, characterized in that 0.5 to 5 parts by weight of a functional substance selected from sugars, mineral mix, and vitamin mix is added in the fourth step process. Pet food manufactured according to any one of claims 1 to 4.